We narrowed to 22,191 results for: his

1. pEW4: pET-32a Dot1L(2-386)

   Plasmid

   #124099

   Purpose

   Dot1L(2-386) expression plasmid

   Depositor

   Cynthia Wolberger

   Article

   Worden et al Cell. 2019 Feb 8. pii: S0092-8674(19)301

   Insert

   Dot1L(2-386) (DOT1L Human)
   

   Use

   Tags

   Trx-His-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   April 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pET28a-Tyr-P(11)4

   Plasmid

   #185784

   Purpose

   Encodes enzyme tyrosinase linked to P(11)4 self-assembling peptide via a GS-linker sequence

   Depositor

   Lizhong He

   Article

   Shanbhag et al Adv. Funct. Mater. 2022, 32, 2108039

   Insert

   Tyr-P(11)4
   

   Use

   Tags

   His-tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   July 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. PM18033-PLS-KP1_rpoA-U

   Plasmid

   #190975

   Purpose

   Test the U-to-C editing activity of KP1 on AtrpoA editing site, in human cells

   Depositor

   Bernard Gutmann

   Article

   Ichinose et al Commun Biol. 2022 Sep 15;5(1):968. doi:

   Insert

   PLS-KP1
   

   Use

   Tags

   His

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Oct. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. NpT7-5-ERK1

   Plasmid

   #39229

   Depositor

   Melanie Cobb

   Article

   Robbins et al J Biol Chem. 1993 Mar 5;268(7):5097-106.

   Insert

   ERK1 (MAPK3 Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   July 15, 2013

   Availability

   Academic Institutions and Nonprofits only

5. pCellFree_G03 TFAM

   Plasmid

   #67064

   Purpose

   Standard for cell-free expression benchmarking.

   Depositor

   Kirill Alexandrov

   Article

   Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

   Insert

   TFAM (TFAM Human)
   

   Use

   Cell-free expression

   Tags

   EGFP and His

   Expression

   Mutation

   S115C

   Promoter

   T7

   Available Since

   Nov. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

6. pQTEV-PRKRA

   Plasmid

   #31571

   Depositor

   Konrad Buessow

   Article

   Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

   Insert

   protein kinase, interferon-inducible double stranded RNA dependent activator (PRKRA Human)
   

   Use

   Tags

   His and TEV

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   March 22, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pGEX-EGFAB(H306Y)

   Plasmid

   #25771

   Depositor

   Johann Deisenhofer

   Article

   McNutt et al J Biol Chem. 2009 Apr 17. 284(16):10561-

   Insert

   LDL receptor EGFAB (LDLR Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   His 306 to Tyr

   Promoter

   Available Since

   Dec. 3, 2010

   Availability

   Academic Institutions and Nonprofits only

8. pGP-CMV-jGCaMP8f F366H

   Plasmid

   #204141

   Purpose

   Fluorescent reporter for calcium imaging in mammalian cells - jGCaMP8f with F366H mutation

   Depositor

   Katalin Torok

   Article

   Tran et al Front Cell Neurosci. 2023 May 18;17:1155

   Insert

   jGCaMP8f F366H
   

   Use

   Tags

   N-terminal His tag

   Expression

   Mammalian

   Mutation

   Phenylalanine 366 changed to histidine

   Promoter

   CMV

   Available Since

   Dec. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. bdSUMO-HA2-GFP-HiBit

   Plasmid

   #171017

   Purpose

   For bacterial expression of bdSUMO tagged muGFP with HA2 peptide and NanoLuc complementation peptide variant #86

   Depositor

   Angus Johnston

   Article

   Teo et al Nat Commun. 2021 Jun 17;12(1):3721. doi:

   Insert

   14x-His-bdSUMO-HA2-muGFP-HiBiT
   

   Use

   Luciferase

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Sept. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET-PLS-KP6_rpoA-C

    Plasmid

    #190966

    Purpose

    Test the C-to-U editing activity of KP6 on AtrpoA editing site, in E. coli

    Depositor

    Bernard Gutmann

    Article

    Ichinose et al Commun Biol. 2022 Sep 15;5(1):968. doi:

    Insert

    PLS-KP6
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Oct. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pET22b-T5-FB-E25TAG-F6C

    Plasmid

    #176546

    Purpose

    Expresses IgG affinity protein FB(F6C) with a TAG stop codon at E25 position

    Depositor

    Han Xiao

    Article

    Yu et al Bioconjug Chem. 2018 Nov 21;29(11):3522-

    Insert

    IgG affinity protein
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    change phenylalanine 6 to cysteine; change glutam…

    Promoter

    T5

    Available Since

    Dec. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. SRS-MTBD(22/19), AB(monomer), wt

    Plasmid

    #22380

    Depositor

    Ron Vale

    Article

    Gibbons et al J Biol Chem. 2005 Jun 24. 280(25):23960-

    Insert

    Seryl tRNA synthetase (SRS) – mouse cytoplasmic dynein microtubule binding domain (MTBD) fusion protein
    

    Use

    Tags

    His and myc

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Nov. 24, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCellFree_G03 ATF1

    Plasmid

    #67065

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    ATF1 (ATF1 Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Expression

    Mutation

    Promoter

    T7

    Available Since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

14. pET16b-Trx C35S

    Plasmid

    #36947

    Depositor

    Anna Blom

    Article

    King et al J Immunol. 2012 Apr 15;188(8):4103-12. E

    Insert

    Thioredoxin-1 C35S mutation (TXN Human)
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    cys 35 to serine

    Promoter

    T7

    Available Since

    May 20, 2013

    Availability

    Academic Institutions and Nonprofits only

15. pET14b_tev_PSGU

    Plasmid

    #59738

    Purpose

    Expresses a.a. 417-887 of human ZO-1

    Depositor

    Alan Fanning

    Article

    Nomme et al J Biol Chem. 2011 Dec 16;286(50):43352-6

    Insert

    zonula occludens-1 (TJP1 Human)
    

    Use

    Tags

    HIS tag and thrombin clvg

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Nov. 5, 2014

    Availability

    Academic Institutions and Nonprofits only

16. pET-sCS4-2T

    Plasmid

    #28250

    Depositor

    Yawen Bai

    Article

    Zhou et al Nature. 2011 Apr 14. 472(7342):234-7.

    Insert

    single chain Cse4-Scm3-H4 with 2 Thrombin site
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    A thrombin cutting site between Cse4 and Scm3. An…

    Promoter

    Available Since

    July 12, 2011

    Availability

    Academic Institutions and Nonprofits only

17. Affibody EGFR:1907.BirA*-pET301/NT-DEST

    Plasmid

    #174691

    Purpose

    Plasmid encoding for the bacterial expression of a bispecific coding for the anti-EGFR affibody ZEGFR:1907 fused to the mutated form of BirA enzyme, BirA*

    Depositor

    Jean Gariepy

    Article

    Alwash et al ACS Pharmacol Transl Sci. 2022 Feb 1;5(2

    Insert

    Anti-EGFR affibody ZEGFR:1907 fused to mutated form of BirA, BirA*
    

    Use

    Tags

    HIS tag

    Expression

    Bacterial

    Mutation

    R118G mutation in BirA

    Promoter

    T7 promoter

    Available Since

    Jan. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. human b1 ecto-pIRES

    Plasmid

    #113387

    Purpose

    Express human beta 1 ectodomain with secretion peptide, purification tags, and C-terminal clasp

    Depositor

    Timothy Springer

    Article

    Li et al EMBO J. 2017 Mar 1;36(5):629-645. doi: 1

    Insert

    human beta 1 ectodomain
    

    Use

    Tags

    His tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Jan. 31, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET28a-Hook3 aa 1-160-GCN4

    Plasmid

    #74608

    Purpose

    Expresses artificially dimerized Hook domain of Hook3 in bacteria for purification

    Depositor

    Ron Vale

    Article

    Schroeder et al J Cell Biol. 2016 Aug 1;214(3):309-18. d

    Insert

    human Hook3 amino acids 1-160 (HOOK3 Human)
    

    Use

    Tags

    6x His, GCN4, Strep II, and superfolder GFP

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Aug. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET28a-TmCA-P(11)4

    Plasmid

    #185783

    Purpose

    Encodes thermostable carbonic anhydrase enzyme linked to P(11)4 self-assembling peptide via a GS-linker sequence

    Depositor

    Lizhong He

    Article

    Shanbhag et al Adv. Funct. Mater. 2022, 32, 2108039

    Insert

    TmCA-P(11)4
    

    Use

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    July 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. PM18033-PLS-KP6_rpoA-U

    Plasmid

    #190987

    Purpose

    Test the U-to-C editing activity of KP6 on AtrpoA editing site, in human cells

    Depositor

    Bernard Gutmann

    Article

    Ichinose et al Commun Biol. 2022 Sep 15;5(1):968. doi:

    Insert

    PLS-KP6
    

    Use

    Tags

    His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Oct. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pAD/CMV/V5-DEST-Rac1-2G

    Plasmid

    #66112

    Purpose

    Second generation Rac1 FRET biosensor for adenovirus production

    Depositor

    Olivier Pertz

    Article

    Fritz et al Dev Cell. 2015 Sep 29. pii: S1534-5807(1

    Insert

    Second generation Rac1 FRET biosensor
    

    Use

    Adenoviral

    Tags

    His-Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Oct. 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pET42b-A3A-PBE-ΔUGI

    Plasmid

    #119771

    Purpose

    To express the recombinant APOBEC3A in vitro

    Depositor

    Caixia Gao

    Article

    Zong et al Nat Biotechnol. 2018 Oct 1. pii: nbt.426

    Insert

    APOBEC3A-nCas9-NLS
    

    Use

    Tags

    6*HIS and NLS

    Expression

    Bacterial

    Mutation

    nCas9 has a mutation D10A in wild type SpCas9

    Promoter

    T7 promoter

    Available Since

    June 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pEBG-TFII-I-delta N90

    Plasmid

    #22165

    Depositor

    Ananda Roy

    Article

    Sacristan et al J Biol Chem. 2004 Feb 20. 279(8):7147-58

    Insert

    TFII-I (GTF2I Human)
    

    Use

    Tags

    GST and His

    Expression

    Mammalian

    Mutation

    The first 90 aa of TFII-I are deleted

    Promoter

    Available Since

    Nov. 13, 2009

    Availability

    Academic Institutions and Nonprofits only

25. pEGFPm-DAD M1041A

    Plasmid

    #25411

    Depositor

    Arthur Alberts

    Article

    Alberts et al J Biol Chem. 2001 Jan 26. 276(4):2824-30

    Insert

    Diap3 (Diaph3 Mouse)
    

    Use

    Tags

    EGFP, His, and Myc

    Expression

    Mammalian

    Mutation

    Alanine substitution at critical residue in DAD t…

    Promoter

    Available Since

    Oct. 6, 2014

    Availability

    Academic Institutions and Nonprofits only

26. spa-GCaMP6s

    Plasmid

    #67556

    Purpose

    photoactivatable fluorescent calcium reporter

    Depositor

    Ehud Isacoff

    Article

    Berlin et al Nat Methods. 2015 Jul 13. doi: 10.1038/n

    Insert

    spa-GCaMP6s
    

    Use

    Tags

    HIS-tag

    Expression

    Mammalian

    Mutation

    K65T, I83V, G87S, Y92D, V115H, K118V, S187R, Y196…

    Promoter

    Available Since

    July 28, 2015

    Availability

    Academic Institutions and Nonprofits only

27. pET-GST-TNRC6A-(935-984)

    Plasmid

    #42050

    Depositor

    Kumiko Ui-Tei

    Article

    Nishi et al RNA. 2013 Jan;19(1):17-35. doi: 10.1261/

    Insert

    TNRC6A (TNRC6A Human)
    

    Use

    Tags

    GST and His

    Expression

    Bacterial

    Mutation

    contain aa935-984

    Promoter

    Available Since

    March 6, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pET28a Gx-NL3

    Plasmid

    #169108

    Purpose

    Expresses protein G fused to 3 copies of NanoLuc. The protein G domain contains an amber stop-codon at position 24 for incorporation of the unnatural amino acid para-benzoylphenylalanine.

    Depositor

    Maarten Merkx

    Article

    Wouters et al Bioconjug Chem. 2020 Mar 18;31(3):656-66

    Insert

    protein G - NanoLuc - NanoLuc - NanoLuc
    

    Use

    Tags

    his-tag and strep-tag

    Expression

    Bacterial

    Mutation

    Ala 24 stop

    Promoter

    T7

    Available Since

    June 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pJOE5751.1

    Plasmid

    #135083

    Purpose

    pBR322-based L-rhamnose-inducible vector.

    Depositor

    Josef Altenbuchner

    Article

    Hoffmann et al FEBS J. 2012 Oct;279(20):3911-24. doi: 1

    Insert

    P-rhaP-BAD-His tag-eGFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Feb. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. mRFP-dmBACCS2

    Plasmid

    #67630

    Purpose

    Drosophila expression of mRFP-dmBACCS2, a Drosophila melanogaster blue light-activated Ca2+ channel switch

    Depositor

    Takao Nakata

    Article

    Ishii et al Nat Commun. 2015 Aug 18;6:8021. doi: 10.

    Insert

    mRFP-dmBACCS2
    

    Use

    Drosophila expression

    Tags

    Expression

    Mutation

    Promoter

    Drosophila actin gene 5C gene promoter

    Available Since

    Aug. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pCellFree_G03 GATA1

    Plasmid

    #67068

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    GATA1 (GATA1 Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Expression

    Mutation

    Promoter

    T7

    Available Since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

32. pDUAL_2_T5

    Plasmid

    #155175

    Purpose

    see pDUAL_T5: exchanged origin of replication (pBR322) and introduced bom site

    Depositor

    Bernd Nidetzky

    Article

    Schwaiger et al Biotechnol J. 2020 Jul 15:e2000063. doi:

    Inserts

    Sucrose Phosphorylase from Bifidobacterium adolescentis (BAD_RS00415 Bifidobacterium adolescentis)
    Cellobiose phosphorylase from Cellulomonas uda
    

    Use

    Synthetic Biology

    Tags

    His Tag and Strep - Tag II

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Sept. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pEBTet-SNAP-FOP

    Plasmid

    #136796

    Purpose

    Mammalian expression of the centrosomal protein FGFR N-terminally fused to SNAP-tag

    Depositor

    Kai Johnsson

    Article

    Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

    Insert

    SNAP-FGFR
    

    Use

    Tags

    FLAG-tag / His-tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-TetO2

    Available Since

    March 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pGEX-4T1-Claspin-N

    Plasmid

    #31623

    Depositor

    Aziz Sancar

    Article

    Lindsey-Boltz et al J Biol Chem. 2009 Nov 27. 284(48):33107-

    Insert

    Claspin (CLSPN Human)
    

    Use

    Tags

    GST and His

    Expression

    Bacterial

    Mutation

    aa 1-679 of N-term

    Promoter

    Available Since

    Nov. 9, 2011

    Availability

    Academic Institutions and Nonprofits only

35. pRSETC-PITPbeta (splice variant 1)

    Plasmid

    #58270

    Purpose

    bacterial expression of PITP beta (phosphatidylinositol transfer protein beta)

    Depositor

    Shamshad Cockcroft

    Article

    Shadan et al Traffic. 2008 Sep;9(10):1743-56. doi: 10

    Insert

    PITP beta (PITPNB Human)
    

    Use

    Tags

    HIS

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    July 15, 2014

    Availability

    Academic Institutions and Nonprofits only

36. pCellFree_G03 GTF2E1

    Plasmid

    #67089

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    GTF2E1 (GTF2E1 Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Expression

    Mutation

    Promoter

    T7

    Available Since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

37. pCellFree_G03 EBNA1BP2

    Plasmid

    #67072

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    EBNA1BP2 EBNA1BP2 EBNA1 binding protein 2 (EBNA1BP2 Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Expression

    Mutation

    Promoter

    T7

    Available Since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

38. pCellFree_G03 HDAC1

    Plasmid

    #67101

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    HDAC1 (HDAC1 Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Expression

    Mutation

    Promoter

    T7

    Available Since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

39. D44_Ld_180090

    Plasmid

    #135858

    Purpose

    Expression of Leishmania donovani ectodomain in mammalian cells

    Depositor

    Gavin Wright

    Article

    Roberts et al PLoS Pathog. 2022 Feb 24;18(2):e1010364.

    Insert

    alpha glucosidase II subunit, putative
    

    Use

    Tags

    biotinylation sequence and His tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. bdSUMO-R9-GFP-HiBit

    Plasmid

    #170990

    Purpose

    For bacterial expression of bdSUMO tagged muGFP with R9 peptide and NanoLuc complementation peptide variant #86

    Depositor

    Angus Johnston

    Article

    Teo et al Nat Commun. 2021 Jun 17;12(1):3721. doi:

    Insert

    14x-His-bdSUMO-R9-muGFP-HiBiT
    

    Use

    Luciferase

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Sept. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge