We narrowed to 8,767 results for: PAN

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pcDNA5-FRT-TO-FH-Nsp2

Plasmid

#157683

Purpose

mammalian expression of N-terminally Flag-His8 tagged SARS-CoV-2 Nsp2 under control of a tetracycline-inducible promoter

Depositor

David Tollervey

Article

Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

Insert

FH-Nsp2 (ORF1ab SARS-CoV-2)

Tags

Flag-His8

Expression

Mammalian

Mutation

codon optimized

Available Since

Aug. 4, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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BII-gR-BtW-eSpCas9

Plasmid

#133358

Purpose

Piggybac vector for CRISPR/SpCas9 applications (nuclease, base editing, or epigenetic modifications). Provides gRNA and EspCas9 expression

Depositor

Blair Madison

Article

Fischer et al Am J Physiol Gastrointest Liver Physiol.

Insert

eSpCas9

Tags

Flag-tagged eSpCas9

Expression

Mammalian

Mutation

eSpCas9 mutations

Promoter

CMV enhancer and hEf1a (CpG free)

Available Since

Jan. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pLV-shKcnk13

Plasmid

#120721

Purpose

Expresses GFP and an shRNA targeting Kcnk13 (in pLL3.7)

Depositor

Amy Lasek

Article

You et al Neuropharmacology. 2018 Oct 14;144:29-36

Insert

Kcnk13 (Kcnk13 Mouse)

Use

Lentiviral and RNAi

Tags

GFP

Expression

Mammalian

Promoter

mouse U6

Available Since

March 12, 2019

Availability

Academic Institutions and Nonprofits only

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FLS2-YFPn

Plasmid

#87165

Purpose

fluorescence complementation assay

Depositor

Silke Robatzek

Article

Frei dit Frey et al Plant Physiol. 2012 Jun;159(2):798-809.

Insert

FLS2 (FLS2 Mustard Weed)

Tags

YFPn

Promoter

35s

Available Since

April 25, 2017

Availability

Academic Institutions and Nonprofits only

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LentiCas9-sgCh2-2-Blast

Plasmid

#199645

Purpose

Expresses Cas9 and sgRNA control guide targeting intergenic region

Depositor

William Hahn

Article

So et al JCI Insight. 2022 Oct 10;7(19):e158755.

Insert

N/A

Use

Lentiviral

Available Since

May 24, 2023

Availability

Academic Institutions and Nonprofits only

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E12-cTAP

Plasmid

#20916

Depositor

Stephen Tapscott

Article

Yang et al Genes Dev. 2009 Mar 15. 23(6):694-707.

Insert

E12 cTAP (Tcf3 Mouse)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Mutation

TAP tag is fused to the 3' end of the E12 ge…

Available Since

May 15, 2009

Availability

Academic Institutions and Nonprofits only
SpIMPDH

Plasmid

#206980

Purpose

Streptococcus pyogenes Inosine 5′-Monophosphate Dehydrogenase (SpIMPDH, EC. 1.1.1.205). Contains His6 tag with TEV protease cleavage site in pET-28b(+) plasmid for protein expression.

Depositor

Vern Schramm

Article

Glockzin et al Biochemistry. 2022 Oct 4;61(19):2088-210

Insert

SpIMPDH (guaB Streptococcus pyogenes)

Tags

His tag (with TEV cleavage site)

Expression

Bacterial

Available Since

March 25, 2024

Availability

Academic Institutions and Nonprofits only

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pYL236

Plasmid

#173184

Purpose

CRISPR donor plasmid for making the WT EZH2 control cell line. It contains a puromycin resistance gene and an mCherry gene

Depositor

Thomas Cech

Article

Long et al Nat Genet. 2020 Sep;52(9):931-938. doi:

Insert

EZH2 (EZH2 Human)

Use

CRISPR

Expression

Mammalian

Available Since

Aug. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pJW1846

Plasmid

#154326

Purpose

Multi-cassette for SapTrap

Depositor

Jordan Ward

Article

Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

Insert

30aa linker::GFP^SEC^AID*::3xFLAG

Use

CRISPR and Cre/Lox

Expression

Worm

Mutation

F+E mutations in sgRNA

Available Since

Aug. 24, 2020

Availability

Academic Institutions and Nonprofits only

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pGL3-Tet1 promoter A

Plasmid

#63881

Purpose

Luciferase reporter plasmid used to study transcriptional control of murine Tet1

Depositor

Kian Peng Koh

Article

Sohni et al Mol Cell Biol. 2015 Mar 15;35(6):1026-42

Insert

Tet1 promoter fragment A

Use

Luciferase

Expression

Mammalian

Available Since

May 19, 2015

Availability

Academic Institutions and Nonprofits only

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Lenti ABE7.10-N-AIDmax

Plasmid

#157949

Purpose

Lentiviral expression of ABE7.10-N-AIDmax

Depositor

Dali Li

Article

Zhang et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038

Insert

Lenti ABE7.10-N-AIDmax

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Dec. 4, 2020

Availability

Academic Institutions and Nonprofits only

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inhibin-DsRed

Plasmid

#156184

Purpose

Expression plasmid for production of transgenic rainbow trout strain carrying the DsRed gene under control of the inhibin α promoter.

Depositor

Goro Yoshizaki

Article

Iwasaki-Takahashi et al Commun Biol. 2020 Jun 15;3(1):308. doi:

Inserts

rainbow trout inhibin α promoter
rainbow trout β actin 3'UTR

Expression

Bacterial

Promoter

inhibin

Available Since

Aug. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pDEST27-GST-ATXN1 FL(30Q)

Plasmid

#21753

Depositor

Huda Zoghbi

Article

Lim et al Nature. 2008 Apr 10. 452(7188):713-8.

Insert

Ataxin-1 (ATXN1 Human)

Tags

GST

Expression

Mammalian

Mutation

insert contains a stretch of 32 amino acids (30 Q…

Promoter

CMV

Available Since

July 18, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA5-FRT-TO-Nsp8-HF

Plasmid

#157696

Purpose

mammalian expression of C-terminally His8-Flag tagged SARS-CoV-2 Nsp8 under control of a tetracycline-inducible promoter

Depositor

David Tollervey

Article

Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

Insert

Nsp8-HF (ORF1ab SARS-CoV-2)

Tags

His8-Flag

Expression

Mammalian

Mutation

codon optimized

Available Since

Aug. 5, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pGL3-Tet1 promoter B

Plasmid

#63882

Purpose

Luciferase reporter plasmid used to study transcriptional control of murine Tet1

Depositor

Kian Peng Koh

Article

Sohni et al Mol Cell Biol. 2015 Mar 15;35(6):1026-42

Insert

Tet1 promoter fragment B

Use

Luciferase

Expression

Mammalian

Available Since

June 26, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT-TO-Nsp7

Plasmid

#157688

Purpose

mammalian expression of untagged SARS-CoV-2 Nsp7 under control of a tetracycline-inducible promoter

Depositor

David Tollervey

Article

Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

Insert

Nsp7 (ORF1ab SARS-CoV-2)

Expression

Mammalian

Mutation

codon optimized

Available Since

Aug. 5, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pRetroX-Tight-Pur-Trim69 isoform B (codon optimized)

Plasmid

#199567

Purpose

Stable and dox. inducible expression of Trim69 isoform B (codon optimized) after retroviral-mediated gene transfer

Depositor

Andrea Cimarelli, Yuxin Song

Article

Song et al Proc Natl Acad Sci U S A. 2022 Oct 25;11

Insert

Trim69 isoform B (codon optimized) (TRIM69 Human)

Use

Retroviral; Allows for puromycin selection

Tags

Flag

Mutation

codon optimized

Available Since

May 4, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX6P1-Ubl-sr1(SIRPT1)

Plasmid

#75298

Purpose

Expresses Sacsin Ubl-sr1 (SIRPT1 repeat) domain (1-339) in fusion with GST (bacterial)

Depositor

Kalle Gehring

Article

Menade et al J Biol Chem. 2018 Aug 17;293(33):12832-1

Insert

Human Sacsin 1-339

Tags

GST-Tag

Expression

Bacterial

Available Since

July 18, 2016

Availability

Academic Institutions and Nonprofits only

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pL90-Nat-2xHO

Plasmid

#139069

Purpose

CRISPR/Cas9 engineering of the HO endonuclease gene with nourseothricin selection

Depositor

Gianni Liti

Article

Barre et al Genome Res. 2020 Apr 10. pii: gr.253351.

Insert

Two guide RNAs targeting the HO endonuclease

Expression

Bacterial and Yeast

Promoter

TDH3

Available Since

May 7, 2020

Availability

Academic Institutions and Nonprofits only

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pAS_4xEcoLeuT(CUA)_EF1_AnapRS

Plasmid

#174894

Purpose

AnapRS expression for amber suppression in mammalian cells; transient or piggy bac mediated integration

Depositor

Simon Elsaesser

Article

Meineke et al Cell Rep Methods. 2023 Nov 20;3(11):1006

Insert

AnapRS

Expression

Mammalian

Mutation

L38F, M40G, L41P, Y499V, Y500L, Y527A, H537E, L53…

Promoter

EF1

Available Since

Nov. 7, 2023

Availability

Academic Institutions and Nonprofits only

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