We narrowed to 14,168 results for: eng

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pAAV-syn-FLEX-axon-jYCaMP1s

Plasmid

#135421

Purpose

Axon-targeted yellow protein calcium sensor expressed under human Synapsin1 promoter, Cre mediated flip-excision switch, for AAV production or direct transfection, slow variant

Depositor

Kaspar Podgorski

Publication

Mohr et al Nat Methods. 2020 May 25. pii: 10.1038/s

Has service

AAV1

Insert

jYCaMP1s

Use

AAV

Tags

GAP43 axon targeting sequence

Expression

Mammalian

Promoter

hSyn

Available since

Jan. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pX330-sgRNA_Ago2_1

Plasmid

#73529

Purpose

Expresses a human codon-optimized SpCas9 and sgRNA targeting Ago2

Depositor

Constance Ciaudo

Publication

Ngondo et al Stem Cell Reports. 2018 Jan 24. pii: S22

Insert

AGO2

Use

CRISPR

Expression

Mammalian

Promoter

hU6

Available since

Feb. 9, 2018

Availability

Academic Institutions and Nonprofits only
pX330-sgRNA_Ago2_2

Plasmid

#73530

Purpose

Expresses a human codon-optimized SpCas9 and sgRNA targeting Ago2

Depositor

Constance Ciaudo

Publication

Ngondo et al Stem Cell Reports. 2018 Jan 24. pii: S22

Insert

AGO2

Use

CRISPR

Expression

Mammalian

Promoter

hU6

Available since

Feb. 9, 2018

Availability

Academic Institutions and Nonprofits only
pSR-puro-Sec5

Plasmid

#31117

Depositor

Christopher Counter

Publication

Issaq et al Mol Cancer Res. 2010 Feb . 8(2):223-31.

Insert

Sec5 shRNA (EXOC2 Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available since

Oct. 3, 2011

Availability

Academic Institutions and Nonprofits only
SOX17-3'gRNA

Plasmid

#210465

Purpose

gRNA targeting SOX17 3' terminal for CRISPR-Cas9-mediated knock-in

Depositor

Kai Wang

Publication

Zhao et al STAR Protoc. 2024 May 23;5(2):103084. do

Insert

SOX17 (SOX17 Human)

Use

CRISPR

Available since

Jan. 11, 2024

Availability

Academic Institutions and Nonprofits only

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AAV_pSyn_Mito-LOV-Turbo-V5

Plasmid

#199669

Purpose

expresses Mito-LOV-Turbo-V5 in neurons, AAV vector

Depositor

Alice Ting

Publication

Lee et al Nat Methods. 2023 May 15. doi: 10.1038/s

Insert

LOV-Turbo

Use

AAV

Tags

COX4I1 mito targeting sequence and V5

Promoter

Synapsin

Available since

May 15, 2023

Availability

Academic Institutions and Nonprofits only

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pTR-177 pUC19-CLTA-N-mCherry

Plasmid

#186128

Purpose

knockin of mCherry to CLTA target

Depositor

Alexander Marson

Publication

Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

Insert

CLTA (CLTA Human)

Use

CRISPR

Mutation

WT with mCherry sequence

Available since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3-AKAP79-Dronpa

Plasmid

#182332

Purpose

AKAP79-targeted Dronpa. Fluctuating green-fluorescent label for live-cell super-resolution imaging.

Depositor

Jin Zhang

Publication

Lin et al J Am Chem Soc. 2021 Sep 22;143(37):14951

Insert

AKAP79-Dronpa (AKAP5 Human)

Tags

Full-length AKAP79

Expression

Mammalian

Promoter

CMV

Available since

July 6, 2022

Availability

Academic Institutions and Nonprofits only

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pDEINO3

Plasmid

#179487

Purpose

Multi-host shuttle plasmid for E. coli, D. radiodurans, and S. cerevisiae; contains an oriT for transfer via conjugation

Depositor

Bogumil Karas

Publication

Brumwell et al ACS Synth Biol. 2022 Mar 18;11(3):1068-1

Insert

TetR/A

Use

Synthetic Biology; Bacterial and yeast cloning

Available since

May 10, 2022

Availability

Academic Institutions and Nonprofits only

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All-in-one split-ADAR2

Plasmid

#170136

Purpose

AAV vector carrying ADAR2-DDC-λN-P2A-MCP-ADAR2-DDN with a nuclear export signal

Depositor

Prashant Mali

Publication

Katrekar et al Elife. 2022 Jan 19;11. pii: 75555. doi:

Insert

ADAR2-DDC-λN-P2A-MCP-ADAR2-DDN

Use

AAV

Expression

Mammalian

Promoter

CMV

Available since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pcDNA5/FRT/TO_H2B-HaloTag11-T2A-EGFP

Plasmid

#175522

Purpose

Nuclear expression of HaloTag11 in mammalian cells

Depositor

Kai Johnsson

Publication

Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

Insert

H2B-HaloTag11

Expression

Mammalian

Mutation

HaloTag11 = HaloTag7-M175W

Available since

Dec. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pMCB619

Plasmid

#171011

Purpose

CRISPR sgRNA vector (puro-BFP with BstXI/BlpI digest sites).

Depositor

Alice Ting

Publication

Coukos et al Elife. 2021 Aug 20;10. pii: 69142. doi:

Insert

U6:sgRNA_Puro-T2A-BFP

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

mU6

Available since

Aug. 19, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pDONR221-FOXQ1

Plasmid

#101628

Purpose

Donor Vector containing FOXQ1 transcription factor, part of the Human TFome Collection

Depositor

George Church

Publication

Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

Insert

FOXQ1 (FOXQ1 Human)

Use

Gateway Cloning

Mutation

Last nucleotide of stop codon removed to allow fo…

Available since

June 3, 2021

Availability

Academic Institutions and Nonprofits only

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pI4-MTU-DO-HPH

Plasmid

#160216

Purpose

Integrative vector for K. marxianus, targeting integration site I4 described in (Rajkumar et al.,2019). Hygromycin selection

Depositor

John Morrissey

Publication

Rajkumar et al Microb Cell Fact. 2020 Nov 11;19(1):207.

Type

Empty backbone

Use

Kluyveromyces marxianus

Expression

Yeast

Promoter

n/a

Available since

Nov. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pI3-MTU-DO-URA

Plasmid

#160020

Purpose

Integrative vector for K. marxianus, targeting integration site I3 described in (Rajkumar et al.,2019). Uracil selection.

Depositor

John Morrissey

Publication

Rajkumar et al Microb Cell Fact. 2020 Nov 11;19(1):207.

Type

Empty backbone

Use

Kluyveromyces marxianus

Expression

Yeast

Promoter

n/a

Available since

Nov. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pMTU-DO-HIS

Plasmid

#160005

Purpose

Low-copy expression vector for K. marxianus, histidine selection

Depositor

John Morrissey

Publication

Rajkumar et al Microb Cell Fact. 2020 Nov 11;19(1):207.

Type

Empty backbone

Use

Kluyveromyces marxianus

Expression

Yeast

Promoter

n/a

Available since

Nov. 12, 2020

Availability

Academic Institutions and Nonprofits only

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Endosome-targeted optodPLD

Plasmid

#140055

Purpose

Encodes CRY2-mCherry-dPLD-P2A-CIBN-2xFYVE for mammalian expression

Depositor

Jeremy Baskin

Publication

Tei et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

Insert

Phospholipase D

Tags

2xFYVE, CIBN, CRY2, P2A, and mCherry

Expression

Mammalian

Mutation

H170A (catalytic dead)

Available since

March 23, 2020

Availability

Academic Institutions and Nonprofits only

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lentiGuide-RRBP1-1

Plasmid

#92156

Purpose

CRISPR guide RNA targeting human RRBP1

Depositor

Alice Ting

Publication

Hung et al Elife. 2017 Apr 25;6. pii: e24463. doi:

Insert

RRBP1 sgRNA-1

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available since

March 15, 2019

Availability

Academic Institutions and Nonprofits only

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pPI304

Plasmid

#113232

Purpose

extrachromosomal expression vector, expression of LifeAct-mCherry/PH-pkgE-GFP

Depositor

Rob Kay

Publication

Paschke et al PLoS One. 2018 May 30;13(5):e0196809. do

Insert

LifeAct & pkgE-PH

Use

Dictyostelium discoideum expression vector, extra…

Tags

mCherry & GFP

Promoter

act15

Available since

Aug. 7, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pSpCas9_BB_2A-GFP_MAPRE1-gRNA#1

Plasmid

#107726

Purpose

Knock out of EB1 in human cells by CRISPR/Cas9

Depositor

Torsten Wittmann

Publication

van Haren et al Nat Cell Biol. 2018 Jan 29. pii: 10.1038

Insert

MAPRE1 gRNA #1 (targets Exon 1) (MAPRE1 Human)

Expression

Mammalian

Promoter

U6

Available since

April 30, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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