We narrowed to 42,665 results for: cha

1. Kv7.5 AB (361-545, Δ395-511)/pET28HMT

   Plasmid

   #111267

   Purpose

   Protein expression in E.Coli

   Depositor

   Dan Minor

   Article

   Chang et al Neuron. 2018 Feb 21;97(4):836-852.e6. do

   Insert

   potassium voltage-gated channel subfamily KQT member 5 isoform 1 [Homo sapiens] (KCNQ5 Human)
   

   Use

   Tags

   His Tag and MBP Tag

   Expression

   Bacterial

   Mutation

   Kv7.5 AB (361-545, Δ395-511)

   Promoter

   T7

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

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2. Kv7.4 (1-645, Δ368-523)/I346A/HA tag/pcDNA3.1

   Plasmid

   #111460

   Purpose

   Electrophysiology in HEK cell

   Depositor

   Dan Minor

   Article

   Chang et al Neuron. 2018 Feb 21;97(4):836-852.e6. do

   Insert

   potassium voltage-gated channel subfamily KQT member 4 isoform a [Homo sapiens] (KCNQ4 Human)
   

   Use

   Tags

   Ha Tag

   Expression

   Mammalian

   Mutation

   Kv7.4 (1-645, Δ368-523)/I346A, C643A

   Promoter

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

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3. Kv7.4 (1-645, Δ368-523)/I346D/HA tag/pcDNA3.1

   Plasmid

   #111461

   Purpose

   Electrophysiology in HEK cell

   Depositor

   Dan Minor

   Article

   Chang et al Neuron. 2018 Feb 21;97(4):836-852.e6. do

   Insert

   potassium voltage-gated channel subfamily KQT member 4 isoform a [Homo sapiens] (KCNQ4 Human)
   

   Use

   Tags

   Ha Tag

   Expression

   Mammalian

   Mutation

   Kv7.4 (1-645, Δ368-523)/I346D, C643A

   Promoter

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

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4. Kv7.4 (1-645, Δ368-523)/S536A/L536A/HA tag/pcDNA3.1

   Plasmid

   #111462

   Purpose

   Electrophysiology in HEK cell

   Depositor

   Dan Minor

   Article

   Chang et al Neuron. 2018 Feb 21;97(4):836-852.e6. do

   Insert

   potassium voltage-gated channel subfamily KQT member 4 isoform a [Homo sapiens] (KCNQ4 Human)
   

   Use

   Tags

   Ha Tag

   Expression

   Mammalian

   Mutation

   Kv7.4 (1-645, Δ368-523)/S536A/L540A, C643A

   Promoter

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

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5. Kv7.4 (1-645, Δ368-523)/S536D/L540D/HA tag/pcDNA3.1

   Plasmid

   #111497

   Purpose

   Electrophysiology in HEK cell

   Depositor

   Dan Minor

   Article

   Chang et al Neuron. 2018 Feb 21;97(4):836-852.e6. do

   Insert

   potassium voltage-gated channel subfamily KQT member 4 isoform a [Homo sapiens] (KCNQ4 Human)
   

   Use

   Tags

   Ha Tag

   Expression

   Mammalian

   Mutation

   Kv7.4 (1-645, Δ368-523)/S536D/L540D, C643A

   Promoter

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

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6. rAAV2-retro helper

   Plasmid

   #81070

   Purpose

   retrograde AAV helper vector, carrying rep and modified capsid gene

   Depositor

   David Schaffer, Alla Karpova

   Article

   Tervo et al Neuron. 2016 Oct 6. pii: S0896-6273(16)3

   Inserts

   rep
   rAAV2-retro cap
   

   Use

   AAV and Unspecified; Helper vector

   Tags

   Expression

   Mutation

   Pro2Ala, possibly Asp613Gly and changes made to A…

   Promoter

   Available since

   Aug. 30, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA5 FRT/TO SF-HERC2 (ShB-R)

   Plasmid

   #55613

   Purpose

   Dox-inducible expression of full length, ShB-resistant, 3xFLAG/STREP tagged wild-type HERC2 in Flp-In TREx cells

   Depositor

   David Chan

   Article

   Chan et al J Biol Chem. 2014 May 22. pii: jbc.M114.

   Insert

   3xFLAG tagged HERC2 (WT) (HERC2 Human)
   

   Use

   Tags

   3xFLAG and STREP

   Expression

   Mammalian

   Mutation

   Silent mutations have been incorporated into the …

   Promoter

   Available since

   July 23, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. U6-hGRIN2B-CAG-ps-SpCas9

   Plasmid

   #102851

   Purpose

   A single-chain light-controllable dSpCas9 with pdDronpa1 domains for hGRIN2B gene editing

   Depositor

   Michael Lin

   Article

   Zhou et al ACS Chem Biol. 2017 Sep 29. doi: 10.1021

   Insert

   sp-hGRIN2B-sgRNA; dSpCas9; pdDronpa1 (GRIN2B Synthetic, Human, S. pyogenes)
   

   Use

   CRISPR

   Tags

   3X Flag and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   U6 promoter

   Available since

   Nov. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. his3MX6-ins3

   Plasmid

   #195040

   Purpose

   pFA6a derived selection cassette flanked with transcription terminators (tDEG1/tCYC1), allows genome modification without disruption of insertion neighboring genes by transcription interference

   Depositor

   Gloria Brar

   Article

   Powers et al Elife. 2022 Dec 12;11:e81086. doi: 10.75

   Insert

   His5+
   

   Use

   Yeast genomic targeting

   Tags

   S. cerevisiae DEG1 terminator, A. gossypii TEF pr…

   Expression

   Mutation

   Promoter

   Available since

   Feb. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. U6-hGRIN2B-CAG-ps-SaCas9

    Plasmid

    #102853

    Purpose

    A single-chain light-controllable dSaCas9 with pdDronpa1 domains for hGRIN2B gene editing

    Depositor

    Michael Lin

    Article

    Zhou et al ACS Chem Biol. 2017 Sep 29. doi: 10.1021

    Insert

    sa-hGRIN2B-sgRNA; dSaCas9; pdDronpa1 (GRIN2B Synthetic, Human, S. aureus)
    

    Use

    CRISPR

    Tags

    3X Flag and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    U6 promoter;

    Available since

    Nov. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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