We narrowed to 29,593 results for: REP

1. pLV-EF1a-NLS-msfGFP-T2A-mCherry-NES> mPGK-PuroR

   Plasmid

   #218916

   Purpose

   Lentiviral construct expressing msfGFP and nuclear mCherry proteins.

   Depositor

   Sven Eyckerman

   Article

   Delhaye et al Cell Rep Methods. 2024 Jul 15;4(7):10081

   Insert

   EF1a-msfGFP-T2A-mCherry-NLS > mPGK-PuroR
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA5/FRT/TO GABARAP(5X/G116A)-mTagBFP2-GABARAP

   Plasmid

   #212107

   Purpose

   Expression of GABARAP split tandem protein with mTagBFP2, 5X: K2A, K13A, R15A, K20A, K23A

   Depositor

   Dieter Willbold

   Article

   Uffing et al Autophagy Rep. 2024 May 16;3(1):2348899.

   Insert

   GABARAP(5X/G116A)-mTagBFP2-GABARAP (GABARAP Human, Entacmaea quadricolor)
   

   Use

   Tags

   mTagBFP2

   Expression

   Mammalian

   Mutation

   5X: K2A, K13A, R15A, K20A, K23A mutations of upst…

   Promoter

   CMV

   Available since

   May 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA5/FRT/TO GABARAP(G116A)-mTagBFP2-GABARAP(G116A)

   Plasmid

   #212108

   Purpose

   Expression of lipidation deficient GABARAP split tandem protein with mTagBFP2

   Depositor

   Dieter Willbold

   Article

   Uffing et al Autophagy Rep. 2024 May 16;3(1):2348899.

   Insert

   GABARAP(G116A)-mTagBFP2-GABARAP(G116A) (GABARAP Human, Entacmaea quadricolor)
   

   Use

   Tags

   mTagBFP2

   Expression

   Mammalian

   Mutation

   G116A (lipidation deficiency) of both GABARAPs

   Promoter

   CMV

   Available since

   May 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDONR221-DDX5(PrD)

   Plasmid

   #166167

   Purpose

   Gateway entry vector for N. furzeri DDX5-PrD(535-634) mutant

   Depositor

   Daniel Jarosz

   Article

   Harel et al Cell Rep. 2024 Jun 25;43(6):112787. doi:

   Insert

   DDX5-PrD(535-634)
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mutation

   PrD only 1606-1902

   Promoter

   Available since

   May 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDONR221-DDX5ΔPrD

   Plasmid

   #166164

   Purpose

   Gateway entry vector for N. furzeri DDX5ΔPrD(1-535) mutant

   Depositor

   Daniel Jarosz

   Article

   Harel et al Cell Rep. 2024 Jun 25;43(6):112787. doi:

   Insert

   DDX5ΔPrD(1-535)
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mutation

   deletion of 1606-1902

   Promoter

   Available since

   May 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDONR221-DDX5ΔIDR

   Plasmid

   #166165

   Purpose

   Gateway entry vector for N. furzeri DDX5ΔIDR(1-483) mutant

   Depositor

   Daniel Jarosz

   Article

   Harel et al Cell Rep. 2024 Jun 25;43(6):112787. doi:

   Insert

   DDX5ΔIDR(1-483)
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mutation

   deletion of 1450-1902

   Promoter

   Available since

   May 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDONR221-DDX5(IDR)

   Plasmid

   #166166

   Purpose

   Gateway entry vector for N. furzeri DDX5-IDR(483-634) mutant

   Depositor

   Daniel Jarosz

   Article

   Harel et al Cell Rep. 2024 Jun 25;43(6):112787. doi:

   Insert

   DDX5-IDR(483-634)
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mutation

   IDR only 1450-1902

   Promoter

   Available since

   May 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAG416GAL-mRuby3

   Plasmid

   #166141

   Purpose

   mRuby3 expression under the control of the Galactose-inducible promoter yeast. Contains CEN/ARS element for low copy within yeast.

   Depositor

   Daniel Jarosz

   Article

   Harel et al Cell Rep. 2024 Jun 25;43(6):112787. doi:

   Insert

   mRuby3
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   GAL1

   Available since

   May 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAG426GAL-ccdB-mRuby3

   Plasmid

   #166142

   Purpose

   Gateway destination vector for generation of Galactose-inductibe, C-terminal mRuby3-tagged proteins in yeast. Contains a 2um element for high copy number when in yeast.

   Depositor

   Daniel Jarosz

   Article

   Harel et al Cell Rep. 2024 Jun 25;43(6):112787. doi:

   Type

   Empty backbone

   Use

   Tags

   mRuby3

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   May 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDEST17-His10-ccdB-SNAP

    Plasmid

    #166146

    Purpose

    Gateway destination vector for generation of N-terminal His10-tagged and C-terminal SNAP-tagged proteins in E. coli.

    Depositor

    Daniel Jarosz

    Article

    Harel et al Cell Rep. 2024 Jun 25;43(6):112787. doi:

    Type

    Empty backbone

    Use

    Tags

    His10 and SNAP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDEST17-His10-Smt3-ccdB

    Plasmid

    #166147

    Purpose

    Gateway destination vector for generation of N-terminal His10-Smt3 tagged proteins in E. coli.

    Depositor

    Daniel Jarosz

    Article

    Harel et al Cell Rep. 2024 Jun 25;43(6):112787. doi:

    Type

    Empty backbone

    Use

    Tags

    His10-Smt3

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDEST17-His10-Smt3-ccdB-SNAP

    Plasmid

    #166148

    Purpose

    Gateway destination vector for generation of N-terminal His10-Smt3 and C-terminal SNAP tagged proteins in E. coli.

    Depositor

    Daniel Jarosz

    Article

    Harel et al Cell Rep. 2024 Jun 25;43(6):112787. doi:

    Type

    Empty backbone

    Use

    Tags

    His10-Smt3 and SNAP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDEST17-His10-Smt3-DDX5(kf)-SNAP

    Plasmid

    #166149

    Purpose

    Expression of N. furzeri DDX5 in E. coli

    Depositor

    Daniel Jarosz

    Article

    Harel et al Cell Rep. 2024 Jun 25;43(6):112787. doi:

    Insert

    DDX5
    

    Use

    Tags

    His10-Smt3 and SNAP

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDEST17-His10-DDX5(kf)-SNAP

    Plasmid

    #166152

    Purpose

    Expression of N. furzeri DDX5 in E. coli

    Depositor

    Daniel Jarosz

    Article

    Harel et al Cell Rep. 2024 Jun 25;43(6):112787. doi:

    Insert

    DDX5
    

    Use

    Tags

    His10 and SNAP

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. CMV-IgK-pHluorin-TM-mRuby

    Plasmid

    #185546

    Purpose

    Expresses surface-localized pHluorin fused to intracellular expression of mRuby under CMV promoter

    Depositor

    Jaime de Juan-Sanz

    Article

    Cuhadar et al Cell Rep. 2024 May 28;43(5):114186. doi:

    Insert

    IgK-pHluorin-TM-mRuby
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLC045 - pL0_CrGLK (CDS1ns)

    Plasmid

    #203904

    Purpose

    MoClo compatible L0 plasmid containing the CrGLK coding sequence, lacking a stop codon to allow for C-terminal fusions.

    Depositor

    Carolyn Lee-Parsons

    Article

    Cole-Osborn et al Plant Cell Rep. 2024 May 14;43(6):141. d

    Insert

    CrGLK (without a stop codon)
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Silent mutation of BpiI or BsaI cut site for MoCl…

    Promoter

    Available since

    April 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLC047 - pL2_p2x35s-CrGLK-tMAS

    Plasmid

    #203906

    Purpose

    Overexpression of the CrGLK coding sequence from C. roseus in a plant binary vector for Agrobacterium-mediated transformation.

    Depositor

    Carolyn Lee-Parsons

    Article

    Cole-Osborn et al Plant Cell Rep. 2024 May 14;43(6):141. d

    Insert

    CrGLK
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Silent mutation of BpiI or BsaI cut site for MoCl…

    Promoter

    CaMV 2x35S

    Available since

    April 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSB278 - pL0_pT16H2 (pro + 5U)

    Plasmid

    #203889

    Purpose

    T16H2 promoter and native 5'UTR (approximately 1 kb upstream of start codon) from C. roseus in a MoClo compatible L0 plasmid.

    Depositor

    Carolyn Lee-Parsons

    Article

    Cole-Osborn et al Plant Cell Rep. 2024 May 14;43(6):141. d

    Insert

    T16H2 promoter and 5'UTR
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Mutation of BpiI or BsaI cut site for MoClo domes…

    Promoter

    Available since

    April 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pUN1224 - pL0_pT3R (pro + 5U)

    Plasmid

    #203892

    Purpose

    T3R promoter and native 5'UTR (approximately 1 kb upstream of start codon) from C. roseus in a MoClo compatible L0 plasmid.

    Depositor

    Carolyn Lee-Parsons

    Article

    Cole-Osborn et al Plant Cell Rep. 2024 May 14;43(6):141. d

    Insert

    T3R promoter and 5'UTR
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Mutation of BpiI or BsaI cut site for MoClo domes…

    Promoter

    Available since

    April 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pUN1215 - pL0_pDAT (pro + 5U)

    Plasmid

    #203895

    Purpose

    DAT promoter and native 5'UTR (approximately 1 kb upstream of start codon) from C. roseus in a MoClo compatible L0 plasmid.

    Depositor

    Carolyn Lee-Parsons

    Article

    Cole-Osborn et al Plant Cell Rep. 2024 May 14;43(6):141. d

    Insert

    DAT promoter and 5'UTR
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Mutation of BpiI or BsaI cut site for MoClo domes…

    Promoter

    Available since

    April 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge