We narrowed to 9,825 results for: 158

1. Topo_SpCas9.tracr_LbCas12a.DR

   Plasmid

   #155049

   Purpose

   TOPO vector for the cloning of _the SpCas9 tracrRNA - LbCas12a Direct Repeat (DR) fragment into the pLCHKO hgRNA vector

   Depositor

   Jason Moffat, Benjamin Blencowe

   Article

   Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

   Insert

   SpCas9 tracrRNA and LbCas12a Direct Repeat (DR)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGEX tensin-SH2

   Plasmid

   #46525

   Depositor

   Bruce Mayer

   Article

   Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

   Insert

   tensin (TNS1 Human)
   

   Use

   Tags

   GST and PreScission

   Expression

   Bacterial

   Mutation

   contains amino acids 1451-1588

   Promoter

   Tac

   Available since

   July 10, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. [pSK497] pLJC5 Flag-Depdc5(AB)

   Plasmid

   #109341

   Purpose

   Lenti-viral expression of Flag-tagged Depdc5 mutant AB

   Depositor

   Kuang Shen, David Sabatini

   Article

   Shen et al Nature. 2018 Apr 5;556(7699):64-69. doi:

   Insert

   Depdc5 (DEPDC5 Human)
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mutation

   DIYGD(185-189)GSGSG;EQPLH(371-375)GSGSG

   Promoter

   Available since

   July 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. AAV-EF1α1.1-SomArchon-GFP

   Plasmid

   #153531

   Purpose

   AAV-mediated expression of SomArchon-GFP under the EF1α1.1 promoter.

   Depositor

   Edward Boyden

   Article

   Piatkevich et al Nature. 2019 Oct;574(7778):413-417. doi:

   Insert

   SomArchon-GFP
   

   Use

   AAV

   Tags

   ER2, GFP, and KGC

   Expression

   Mammalian

   Mutation

   Promoter

   EF1α1.1

   Available since

   Feb. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. C-His-GvpC-TEV

   Plasmid

   #153296

   Purpose

   Expresses TEV-sensitive variant of Anabaena flos-aquae GvpC in E.coli

   Depositor

   Mikhail Shapiro

   Article

   Lakshmanan et al Nat Chem Biol. 2020 Sep;16(9):988-996. d

   Insert

   TEV-sensitive Gas vesicle protein C (his-tagged)
   

   Use

   Tags

   His-tag

   Expression

   Bacterial

   Mutation

   1) 21 bp TEV recognition and cleavage site flanke…

   Promoter

   T7

   Available since

   Sept. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. N-His-GvpC-ssrA

   Plasmid

   #153297

   Purpose

   Expresses ClpXP-degradable variant of Anabaena flos-aquae GvpC in E.coli

   Depositor

   Mikhail Shapiro

   Article

   Lakshmanan et al Nat Chem Biol. 2020 Sep;16(9):988-996. d

   Insert

   Gas vesicle protein C (with N-terminal his-tag and C-terminal ssrA tag)
   

   Use

   Tags

   His-tag and ssrA tag

   Expression

   Bacterial

   Mutation

   1) N-terminal 6xHis tag flanked by 1x glycine res…

   Promoter

   T7

   Available since

   Sept. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGEX4T1-Rpb1-linker

   Plasmid

   #138468

   Purpose

   Expresses GST-tagged Rpb1 linker in bacteria

   Depositor

   Britt Glaunsinger

   Article

   Castaneda et al PLoS Pathog. 2020 Sep 4;16(9):e1008843.

   Insert

   Rpb1 (POLR2A Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Amino acids 1460-1585

   Promoter

   tac

   Available since

   Oct. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pKD251

   Plasmid

   #124714

   Purpose

   Expression of torS under Ptac and torT under PD59_luxR

   Depositor

   Jeffrey Tabor

   Article

   Schmidl et al Nat Chem Biol. 2019 Jul;15(7):690-698. d

   Inserts

   torS (torS )
   torT (torS )
   lacI (lacI )
   luxR
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. Topo_SpCas9.tracr_AsCas12a.DR

   Plasmid

   #155050

   Purpose

   TOPO vector for the cloning of _the SpCas9 tracrRNA - AsCas12a Direct Repeat (DR) fragment into the pLCHKO hgRNA vector

   Depositor

   Jason Moffat, Benjamin Blencowe

   Article

   Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

   Insert

   SpCas9 tracrRNA and AsCas12a Direct Repeat (DR)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCR1110

    Plasmid

    #111096

    Purpose

    Expresses BLM_+_91260645.23-P1P2 sgRNA in pCR1068

    Depositor

    Jacob Corn

    Article

    Richardson et al Nat Genet. 2018 Aug;50(8):1132-1139. doi

    Insert

    BLM gRNA (BLM Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCR1322

    Plasmid

    #111109

    Purpose

    Expresses FANCL_+_58468435.23-P1P2 sgRNA in pCR1068

    Depositor

    Jacob Corn

    Article

    Richardson et al Nat Genet. 2018 Aug;50(8):1132-1139. doi

    Insert

    FANCL gRNA (FANCL Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCR1271

    Plasmid

    #111106

    Purpose

    Expresses FANCI_-_89787239.23-P1P2 sgRNA in pCR1068

    Depositor

    Jacob Corn

    Article

    Richardson et al Nat Genet. 2018 Aug;50(8):1132-1139. doi

    Insert

    FANCI gRNA (FANCI Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. SPHK2 gRNA (BRDN0001146882)

    Plasmid

    #77096

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human SPHK2

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    SPHK2 (SPHK2 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. P022-mPB-TRE-PEmax-T2A-mCherry-PGK-rttA-Puro

    Plasmid

    #239974

    Purpose

    PiggyBac plasmid for inducible PEmax expression and Puromycin selection

    Depositor

    Jay Shendure

    Article

    Chen et al Nature. 2024 Aug;632(8027):1073-1081. do

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE

    Available since

    June 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. P021-mPB-ENGRAM-All-in-one-PGK-BFP-P2A-Blast

    Plasmid

    #239972

    Purpose

    Updated ENGRAM plasmid that is ready for both 5' and 3'-FT ENGRAM cloning

    Depositor

    Jay Shendure

    Article

    Chen et al Nature. 2024 Aug;632(8027):1073-1081. do

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pcDNA3.1-HaloCKAR2.2-T2A-EGFP

    Plasmid

    #216493

    Purpose

    Expression of chemigenetic PKC biosensor (low basal brightness, extremly high dynamic range) and EGFP transfection marker in mammalian cells

    Depositor

    Jin Zhang

    Article

    Frei et al Nat Biotechnol. 2025 Apr 21. doi: 10.103

    Insert

    HaloCKAR2.2
    

    Use

    Tags

    T2A-EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcDNA3.1-HaloAktKAR2.2-T2A-EGFP

    Plasmid

    #216494

    Purpose

    Expression of chemigenetic Akt biosensor (low basal brightness, extremly high dynamic range) and EGFP transfection marker in mammalian cells

    Depositor

    Jin Zhang

    Article

    Frei et al Nat Biotechnol. 2025 Apr 21. doi: 10.103

    Insert

    HaloAktKAR2.2
    

    Use

    Tags

    T2A-EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA3.1-HaloEKAR2.2-T2A-EGFP

    Plasmid

    #216495

    Purpose

    Expression of chemigenetic ERK biosensor (low basal brightness, extremly high dynamic range) and EGFP transfection marker in mammalian cells

    Depositor

    Jin Zhang

    Article

    Frei et al Nat Biotechnol. 2025 Apr 21. doi: 10.103

    Insert

    HaloEKAR2.2
    

    Use

    Tags

    T2A-EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA3.1-HaloAKAR2.0-T2A-EGFP

    Plasmid

    #216488

    Purpose

    Expression of chemigenetic PKA biosensor (high basal brightness, medium dynamic range) and EGFP transfection marker in mammalian cells

    Depositor

    Jin Zhang

    Article

    Frei et al Nat Biotechnol. 2025 Apr 21. doi: 10.103

    Insert

    HaloAKAR2.0
    

    Use

    Tags

    T2A-EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA3.1-HaloAKAR2.1-T2A-EGFP

    Plasmid

    #216489

    Purpose

    Expression of chemigenetic PKA biosensor (medium basal brightness, high dynamic range) and EGFP transfection marker in mammalian cells

    Depositor

    Jin Zhang

    Article

    Frei et al Nat Biotechnol. 2025 Apr 21. doi: 10.103

    Insert

    HaloAKAR2.1
    

    Use

    Tags

    T2A-EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pcDNA3.1-HaloAKAR2.2-T2A-EGFP

    Plasmid

    #216490

    Purpose

    Expression of chemigenetic PKA biosensor (low basal brightness, extremly high dynamic range) and EGFP transfection marker in mammalian cells

    Depositor

    Jin Zhang

    Article

    Frei et al Nat Biotechnol. 2025 Apr 21. doi: 10.103

    Insert

    HaloAKAR2.2
    

    Use

    Tags

    T2A-EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pcDNA3.1-HaloAKAR2.1-NES-T2A-EGFP-CAAX

    Plasmid

    #216491

    Purpose

    Expression of chemigenetic PKA biosensor (medium basal brightness, high dynamic range) and PM targeted EGFP in mammalian cells

    Depositor

    Jin Zhang

    Article

    Frei et al Nat Biotechnol. 2025 Apr 21. doi: 10.103

    Insert

    HaloAKAR2.1
    

    Use

    Tags

    NES, T2A-EGFP-CAAX

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pcDNA3-Lyso-Digda

    Plasmid

    #236102

    Purpose

    Lysosome-targeted FRET-based biosensor for monitoring diacylglycerol (DAG) dynamics in living cells.

    Depositor

    Jin Zhang

    Article

    Su et al Nat Chem Biol. 2024 Oct 11. doi: 10.1038

    Insert

    Lyso-Digda (LAMP1 Synthetic, Human)
    

    Use

    Tags

    Full-length LAMP1

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pGEMHE-MmBorealin-OLLAS

    Plasmid

    #237439

    Purpose

    Expresses mouse Borealin tagged with OLLAS at C-term; made for in vitro transcription (T7 promoter)

    Depositor

    Michael Lampson

    Article

    Dudka et al Nature. 2025 Feb;638(8051):814-822. doi:

    Insert

    Borealin (Cdca8 Mouse)
    

    Use

    Tags

    OLLAS

    Expression

    Mammalian

    Mutation

    Promoter

    T7

    Available since

    June 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pPB_TRE3G_scFV-sfGFP-Dot1LCD-CatMut

    Plasmid

    #235591

    Purpose

    Dox-inducible expression of control catalytic-mutant Dot1l CD fused with scFV

    Depositor

    Jamie Hackett

    Article

    Policarpi et al Nat Genet. 2024 Jun;56(6):1168-1180. doi

    Insert

    Dot1l (Dot1l Mouse)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    GS163-164RC

    Promoter

    Available since

    April 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pGL3-Myc-rDMRdeltaTEAD

    Plasmid

    #235695

    Purpose

    Measure the activity of the reduced differentially methylated region (rDMR) of Myc lacking Tead-binding motif with luciferase assay

    Depositor

    Duojia Pan

    Article

    Wu et al Nat Genet. 2022 Aug;54(8):1202-1213. doi

    Insert

    Myc (Myc Mouse)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pHAGE Flag HA ITPA

    Plasmid

    #237998

    Purpose

    Inducible lentiviral expression of ITPA

    Depositor

    David Pellman

    Article

    Tang et al Nature. 2022 Jun;606(7916):930-936. doi:

    Insert

    ITPA (ITPA Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pHAGE Flag HA MPG

    Plasmid

    #237804

    Purpose

    Lentiviral vector expressing WT MPG

    Depositor

    David Pellman

    Article

    Tang et al Nature. 2022 Jun;606(7916):930-936. doi:

    Insert

    MPG (MPG Human)
    

    Use

    Tags

    Flag 3HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pHAGE Flag HA MPG Catalytic Inactive

    Plasmid

    #237807

    Purpose

    Lentiviral vector expressing Catalytic Inactive MPG

    Depositor

    David Pellman

    Article

    Tang et al Nature. 2022 Jun;606(7916):930-936. doi:

    Insert

    MPG (MPG Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pGL3-Cyr61-rDMR

    Plasmid

    #235692

    Purpose

    Measure the activity of the reduced differentially methylated region (rDMR) of Cyr61 with luciferase assay

    Depositor

    Duojia Pan

    Article

    Wu et al Nat Genet. 2022 Aug;54(8):1202-1213. doi

    Insert

    Cyr61 (Ccn1 Mouse)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge