We narrowed to 9,129 results for: Pol

1. pGoldyTALEN-TUBA1B-TAL-2

   Plasmid

   #62854

   Purpose

   TALEN mediated gene editing at human TUBA1B locus

   Depositor

   Gaudenz Danuser

   Article

   Gan et al Cell Syst. 2016 Sep 21. pii: S2405-4712(

   Insert

   TALEN array
   

   Use

   TALEN

   Expression

   Mammalian

   Promoter

   miniCAGG

   Available Since

   May 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGoldyTALEN-TUBA1B-TAL-1

   Plasmid

   #62853

   Purpose

   TALEN mediated gene editing at human TUBA1B locus

   Depositor

   Gaudenz Danuser

   Article

   Gan et al Cell Syst. 2016 Sep 21. pii: S2405-4712(

   Insert

   TALEN array
   

   Use

   TALEN

   Expression

   Mammalian

   Promoter

   miniCAGG

   Available Since

   May 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. mouse Cavin-1

   Plasmid

   #50441

   Purpose

   expresses mouse Cavin-1 in mammalian cells

   Depositor

   Paul Pilch

   Article

   Liu et al J Biol Chem. 2008 Feb 15;283(7):4314-22.

   Insert

   Cavin-1 (Cavin1 Mouse)
   

   Tags

   HA

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 2, 2014

   Availability

   Academic Institutions and Nonprofits only

4. pBAD-BpBBS

   Plasmid

   #199467

   Purpose

   Arabinose-induced expression of His-tagged biliverdin-binding serpin of Boana punctata treefrog (BpBBS) in E. coli.

   Depositor

   Vladislav Verkhusha

   Article

   Manoilov et al J Mol Biol. 2022 Jan 30;434(2):167359. d

   Insert

   BpBBS
   

   Tags

   6xHis

   Expression

   Bacterial

   Available Since

   June 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. R10-3 /pQE-30

   Plasmid

   #79859

   Purpose

   The first mutant of the Aequorea victoria green fluorescent protein that forms a red chromophore.

   Depositor

   Konstantin Lukyanov

   Article

   Mishin et al Biochemistry. 2008 Apr 22;47(16):4666-73

   Insert

   R10-3
   

   Tags

   HisTag

   Expression

   Bacterial

   Mutation

   Aequorea victoria green fluorescent protein (avGF…

   Promoter

   T5 promoter / lac operator

   Available Since

   Sept. 26, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. B108-Otx2-IRES-Cre

   Plasmid

   #73993

   Purpose

   B108 enhancer of Blimp1 gene drives Otx2 (isoform b) and Cre expression.

   Depositor

   Connie Cepko

   Article

   Wang et al Dev Cell. 2014 Sep 8;30(5):513-27. doi:

   Insert

   B108-Otx2 (isoform b)-IRES-Cre (Otx2 Mouse)
   

   Expression

   Mammalian

   Available Since

   July 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. GAL 103Q+ProCFPp303

   Plasmid

   #15575

   Depositor

   Susan Lindquist

   Article

   Duennwald et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

   Insert

   htt103Q + Pro (HTT Human)
   

   Tags

   CFP

   Expression

   Yeast

   Mutation

   Proline-rich region present

   Available Since

   Aug. 15, 2007

   Availability

   Academic Institutions and Nonprofits only

8. pCMV5 Flag-Par6 delta 250-295 Flag tagged

   Plasmid

   #24646

   Depositor

   Jeff Wrana

   Article

   Narimatsu et al Cell. 2009 Apr 17. 137(2):295-307.

   Insert

   Par6c (Pard6a Mouse)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Deletion of the PDZ domain of Par6c (aa 250-295)

   Available Since

   June 2, 2010

   Availability

   Industry, Academic Institutions, and Nonprofits

9. pMSCV PIG IMP-1-long-mut

   Plasmid

   #21661

   Depositor

   David Bartel

   Article

   Mayr et al Cell. 2009 Aug 21. 138(4):673-84.

   Insert

   IGF-II mRNA-binding protein 1 (IGF2BP1 Human)
   

   Use

   Retroviral

   Tags

   GFP and IRES

   Expression

   Mammalian

   Mutation

   mutated let-7 binding sites.

   Available Since

   July 28, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBS drosophila armadillo

    Plasmid

    #16829

    Depositor

    Eric Wieschaus

    Article

    Peifer et al Cell. 1990 Dec 21. 63(6):1167-76.

    Insert

    armadillo (arm Fly)
    

    Expression

    Bacterial

    Available Since

    June 4, 2008

    Availability

    Academic Institutions and Nonprofits only

11. pCellFree_G03 HDAC1

    Plasmid

    #67101

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    HDAC1 (HDAC1 Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Promoter

    T7

    Available Since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

12. Flag-ATXN1_V591A_S602D

    Plasmid

    #48190

    Purpose

    Expresses V591A and S602D mutated Ataxin1

    Depositor

    Huda Zoghbi

    Article

    Kim et al Genes Dev. 2013 Mar 15;27(6):590-5. doi:

    Insert

    Ataxin1 (ATXN1 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 23, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. Flag-ATXN1_V591A_L686E

    Plasmid

    #48191

    Purpose

    Expresses V591A and L686E mutated Ataxin1

    Depositor

    Huda Zoghbi

    Article

    Kim et al Genes Dev. 2013 Mar 15;27(6):590-5. doi:

    Insert

    Ataxin1 (ATXN1 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 23, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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14. Flag-ATXN1_S602D_L686E

    Plasmid

    #48192

    Purpose

    Expresses S602D and L686E mutated Ataxin1

    Depositor

    Huda Zoghbi

    Article

    Kim et al Genes Dev. 2013 Mar 15;27(6):590-5. doi:

    Insert

    Ataxin1 (ATXN1 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 21, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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15. p21 promoter E

    Plasmid

    #16466

    Depositor

    Bert Vogelstein

    Article

    el-Deiry et al Cancer Res. 1995 Jul 1. 55(13):2910-9.

    Insert

    p21 promoter (CDKN1A Human)
    

    Expression

    Mammalian

    Available Since

    April 7, 2008

    Availability

    Academic Institutions and Nonprofits only

16. pY71-tet.I.8

    Plasmid

    #133521

    Purpose

    sfGFP driven by a T7 promoter with the tetO sequence 11 bp downstream from the promoter start site

    Depositor

    Richard Murray

    Article

    McManus et al Arch Biochem Biophys. 2019 Jul 18. pii:

    Insert

    sfGFP
    

    Tags

    Strep tag and TEV site

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    T7

    Available Since

    Dec. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. p5A0-T

    Plasmid

    #92016

    Purpose

    Expresses TALE targeted to lac operator without TEV cut sites, anhydrotetracycline inducible TEV protease

    Depositor

    Brian Pfleger

    Article

    Copeland et al Nat Chem Biol. 2016 Apr;12(4):254-60. do

    Inserts

    TALE targeting lac operator
    Tobacco Etch Virus Protease
    

    Use

    TALEN

    Tags

    5x Arginine Tag, 6x His Tag, 7x His Tag, and FLAG…

    Mutation

    S219V

    Promoter

    J23102 Promoter (from Anderson promoter library) …

    Available Since

    Jan. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pEGFP ratAPOBEC1 L173A/G227A

    Plasmid

    #167169

    Purpose

    Expresses rat APOBEC1 L173A/G227A in mammalian cells. Please note that this does not express GFP.

    Depositor

    Silvestro Conticello

    Article

    Chieca et al bioRxiv 2021

    Insert

    APOBEC1 (Apobec1 Rat)
    

    Expression

    Mammalian

    Mutation

    changed Leucine 173 to Alanine; changed Glycine 2…

    Promoter

    CMV promoter

    Available Since

    May 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pIC389

    Plasmid

    #37387

    Depositor

    Iain Cheeseman

    Article

    Kiyomitsu et al Nat Cell Biol. 2012 Feb 12;14(3):311-7.

    Insert

    LGN (GPSM2 Human)
    

    Use

    Retroviral

    Tags

    GFP, S-tag, and TEV

    Expression

    Mammalian

    Mutation

    deleted amino acid 1-364 and 524

    Available Since

    July 19, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA pDEST40 Cas9 GW STOP

    Plasmid

    #183196

    Purpose

    Destination vector

    Depositor

    Emma Haapaniemi

    Article

    Reint et al Elife. 2021 Dec 13;10. pii: 75415. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    May 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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