We narrowed to 7,201 results for: mme

1. PX459-CTNNA1(α-catenin CRISPR KO)

   Plasmid

   #229708

   Purpose

   Knockout of a-catenin in mammalian cells using CRISPR-Cas9 technology

   Depositor

   Alpha Yap

   Article

   Noordstra et al Dev Cell. 2023 Sep 25;58(18):1748-1763.e

   Insert

   CTNNA1 (alpha-catenin) Human (CTNNA1 Human)
   

   Expression

   Mammalian

   Promoter

   CMV promoter

   Available Since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. GFP-α-cateninV-

   Plasmid

   #229696

   Purpose

   Transient expression of GFP-alpha-cateninV- in mammalian cells

   Depositor

   Alpha Yap

   Article

   Noordstra et al Dev Cell. 2023 Sep 25;58(18):1748-1763.e

   Insert

   CTNNA1 (alpha-catenin) Human (CTNNA1 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   L344P

   Promoter

   CMV promoter

   Available Since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRSF_his6_sumo_hRNMT_RAM

   Plasmid

   #213528

   Purpose

   Expresses the human RNMT-RAM complex

   Depositor

   Thomas Tuschl

   Article

   Meyer et al Nature. 2024 Dec 11. doi: 10.1038/s41586

   Inserts

   human RNMT (RNMT Human)
   RAM
   

   Tags

   His6-sumo

   Expression

   Bacterial

   Available Since

   Dec. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRSF_his6_sumo_SARS_CoV2_NSP14

   Plasmid

   #213518

   Purpose

   Expresses NSP14 of SARS-CoV-2

   Depositor

   Thomas Tuschl

   Article

   Meyer et al Nature. 2024 Dec 11. doi: 10.1038/s41586

   Insert

   SARS-CoV-2 NSP14 (ORF1ab SARS-CoV-2)
   

   Tags

   N-terminal His6-sumo

   Expression

   Bacterial

   Available Since

   Dec. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRSF_his6_sumo_SARS_CoV2_NSP14_V290A

   Plasmid

   #213522

   Purpose

   Expresses NSP14 of SARS-CoV-2 with a single point mutation V290A

   Depositor

   Thomas Tuschl

   Article

   Meyer et al Nature. 2024 Dec 11. doi: 10.1038/s41586

   Insert

   SARS-CoV-2 NSP14 (ORF1ab SARS-CoV-2)
   

   Tags

   His6-sumo

   Expression

   Bacterial

   Mutation

   changed V290 to A

   Available Since

   Dec. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRSF_his6_sumo_SARS_CoV2_NSP14_N306K

   Plasmid

   #213523

   Purpose

   Expresses NSP14 of SARS-CoV-2 with a single point mutation N306K

   Depositor

   Thomas Tuschl

   Article

   Meyer et al Nature. 2024 Dec 11. doi: 10.1038/s41586

   Insert

   SARS-CoV2-NSP14 (ORF1ab SARS-CoV-2)
   

   Tags

   His6-sumo

   Expression

   Bacterial

   Mutation

   changed N306 to K

   Available Since

   Dec. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pRSF_his6_sumo_SARS_CoV2_NSP14_P335S

   Plasmid

   #213524

   Purpose

   Expresses NSP14 of SARS-CoV-2 with a single point mutation P335S

   Depositor

   Thomas Tuschl

   Article

   Meyer et al Nature. 2024 Dec 11. doi: 10.1038/s41586

   Insert

   SARS-CoV-2 NSP14 (ORF1ab SARS-CoV-2)
   

   Tags

   His6-sumo

   Expression

   Bacterial

   Mutation

   changed P335 to S

   Available Since

   Dec. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pRSF_his6_sumo_SARS_CoV2_NSP14_P335H

   Plasmid

   #213525

   Purpose

   Expresses NSP14 of SARS-CoV-2 with a single point mutation P335H

   Depositor

   Thomas Tuschl

   Article

   Meyer et al Nature. 2024 Dec 11. doi: 10.1038/s41586

   Insert

   SARS-CoV-2 NSP14 (ORF1ab SARS-CoV-2)
   

   Tags

   His6-sumo

   Expression

   Bacterial

   Mutation

   changed P335 to H

   Available Since

   Dec. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRSF_his6_sumo_SARS_CoV2_NSP14_H424R

   Plasmid

   #213526

   Purpose

   Expresses NSP14 of SARS-CoV-2 with a single point mutation H424R

   Depositor

   Thomas Tuschl

   Article

   Meyer et al Nature. 2024 Dec 11. doi: 10.1038/s41586

   Insert

   SARS-CoV-2 NSP14 (ORF1ab SARS-CoV-2)
   

   Tags

   His6-sumo

   Expression

   Bacterial

   Mutation

   changed H424 to R

   Available Since

   Dec. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. VC-Y705F+K49R-STAT3

    Plasmid

    #203721

    Purpose

    Expresses the PTM-resistant, double mutant Y705F+K49R - STAT3 fused to the 158-238 fragment of Venus in mammalian cells

    Depositor

    Federico Herrera

    Article

    Diallo et al Cell Oncol (Dordr). 2023 Dec 27. doi: 10

    Insert

    STAT3 tagged with VC (aa 158-238) (STAT3 Human)
    

    Tags

    VC (aa 158-238) for bimolecular fluorescence comp…

    Expression

    Mammalian

    Mutation

    Y705F+K49R substitutions

    Promoter

    CMV

    Available Since

    Dec. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. VC-Y705F+K685R-STAT3

    Plasmid

    #203722

    Purpose

    Expresses the PTM-resistant, double mutant Y705F+K685R - STAT3 fused to the 158-238 fragment of Venus in mammalian cells

    Depositor

    Federico Herrera

    Article

    Diallo et al Cell Oncol (Dordr). 2023 Dec 27. doi: 10

    Insert

    STAT3 tagged with VC (aa 158-238) (STAT3 Human)
    

    Tags

    VC (aa 158-238) for bimolecular fluorescence comp…

    Expression

    Mammalian

    Mutation

    Y705F+K685R substitutions

    Promoter

    CMV

    Available Since

    Dec. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSLED.CaRPv1

    Plasmid

    #193018

    Purpose

    Excitatory/Inhibitory neuron calcium indicator (hSyn promoter, Synrg exon, jRGECO1a/jGCaMP7b)

    Depositor

    Jonathan Ling

    Article

    Ling et al Nat Commun. 2022 Oct 1;13(1):5773. doi:

    Insert

    Bichromatic calcium indicator (jGCaMP7b and jRGECO1a)
    

    Use

    AAV

    Available Since

    Dec. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSLED.RAB

    Plasmid

    #193015

    Purpose

    Photoreceptor-specific reporter (CBh promoter, Atp1b2 exon, bichromatic reporter)

    Depositor

    Jonathan Ling

    Article

    Ling et al Nat Commun. 2022 Oct 1;13(1):5773. doi:

    Insert

    Bichromatic reporter (EGFP and dsRed-Express2)
    

    Use

    AAV

    Available Since

    Dec. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLY315

    Plasmid

    #184160

    Purpose

    Reporter circuit for hijacking mRNA of zntA as CRISPR RNA

    Depositor

    Baojun Wang

    Article

    Liu et al Nat Commun. 2022 Apr 11;13(1):1937. doi:

    Inserts

    dCas9
    tetR
    pspFΔHTH::λN22plus
    sfgfp
    rhaS
    

    Use

    Synthetic Biology

    Tags

    ASV tag and λN22plus

    Expression

    Bacterial

    Mutation

    Mutations D10A and H840A on WT cas9. Synonymous m…

    Promoter

    Pcon, PpspA-zntA-1034, PrhaB, and Ptet

    Available Since

    Dec. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLY162

    Plasmid

    #184138

    Purpose

    Reporter circuit for hijacking RFP mRNA as CRISPR RNA (target site 1)

    Depositor

    Baojun Wang

    Article

    Liu et al Nat Commun. 2022 Apr 11;13(1):1937. doi:

    Inserts

    dCas9
    tetR
    pspFΔHTH::λN22plus
    sfgfp
    rhaS
    

    Use

    Synthetic Biology

    Tags

    ASV tag and λN22plus

    Expression

    Bacterial

    Mutation

    Mutations D10A and H840A on WT cas9. Synonymous m…

    Promoter

    Pcon, PpspA-R1, PrhaB, and Ptet

    Available Since

    Oct. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLY163

    Plasmid

    #184141

    Purpose

    Reporter circuit for hijacking RFP mRNA as CRISPR RNA (target site 2)

    Depositor

    Baojun Wang

    Article

    Liu et al Nat Commun. 2022 Apr 11;13(1):1937. doi:

    Inserts

    dCas9
    tetR
    pspFΔHTH::λN22plus
    sfgfp
    rhaS
    

    Use

    Synthetic Biology

    Tags

    ASV tag and λN22plus

    Expression

    Bacterial

    Mutation

    Mutations D10A and H840A on WT cas9. Synonymous m…

    Promoter

    Pcon, PpspA-R2, PrhaB, and Ptet

    Available Since

    Oct. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLY168

    Plasmid

    #184142

    Purpose

    Reporter circuit for hijacking mRNA of arsenic responsive gene cluster as CRISPR RNA (site 2)

    Depositor

    Baojun Wang

    Article

    Liu et al Nat Commun. 2022 Apr 11;13(1):1937. doi:

    Inserts

    dCas9
    tetR
    pspFΔHTH::λN22plus
    sfgfp
    rhaS
    

    Use

    Synthetic Biology

    Tags

    ASV tag and λN22plus

    Expression

    Bacterial

    Mutation

    Mutations D10A and H840A on WT cas9. Synonymous m…

    Promoter

    Pcon, PpspA-Ar2, PrhaB, and Ptet

    Available Since

    Oct. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLY300

    Plasmid

    #184158

    Purpose

    Reporter circuit for hijacking small RNA oxyS as CRISPR RNA

    Depositor

    Baojun Wang

    Article

    Liu et al Nat Commun. 2022 Apr 11;13(1):1937. doi:

    Inserts

    dCas9
    tetR
    pspFΔHTH::λN22plus
    sfgfp
    rhaS
    

    Use

    Synthetic Biology

    Tags

    ASV tag and λN22plus

    Expression

    Bacterial

    Mutation

    Mutations D10A and H840A on WT cas9. Synonymous m…

    Promoter

    Pcon, PpspA-oxyS-86, PrhaB, and Ptet

    Available Since

    Oct. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLY320

    Plasmid

    #184161

    Purpose

    Reporter circuit for hijacking mRNA of cusCFAB as CRISPR RNA

    Depositor

    Baojun Wang

    Article

    Liu et al Nat Commun. 2022 Apr 11;13(1):1937. doi:

    Inserts

    dCas9
    tetR
    pspFΔHTH::λN22plus
    sfgfp
    rhaS
    

    Use

    Synthetic Biology

    Tags

    ASV tag and λN22plus

    Expression

    Bacterial

    Mutation

    Mutations D10A and H840A on WT cas9. Synonymous m…

    Promoter

    Pcon, PpspA-cusCFAB-656, PrhaB, and Ptet

    Available Since

    Oct. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pGFP-nE-mHP1alpha

    Plasmid

    #181895

    Purpose

    Fluorescently tagged HP1alpha, serines in N-terminal extension (NTE) replaced by glutamates

    Depositor

    Karsten Rippe

    Article

    Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

    Insert

    nE-mHP1alpha (Cbx5 Mouse)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    S11E, S12E, S13E, S14E

    Promoter

    CMV

    Available Since

    Sept. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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