We narrowed to 5,561 results for: Chia

1. pHDAdVenus-geo-TK

   Plasmid

   #26423

   Depositor

   Kohnosuke Mitani

   Article

   Suzuki et al Proc Natl Acad Sci U S A. 2008 Sep 16. 1

   Insert

   PGK-b-geo, HSV-TK, CAG-Venus
   

   Use

   Adenoviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 31, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDOC-GG

   Plasmid

   #149377

   Purpose

   Chassis plasmid for building mutagenesis cassettes by Golden Gate assembly to form a donor plasmid for recombineering by Gene Doctoring

   Depositor

   Mark Pallen

   Article

   Thomson et al BMC Biotechnol. 2020 Oct 7;20(1):54. doi

   Inserts

   Fragment containing a kanamycin resistance cassette
   Fragment containing pMB1 origin of replication and an I-SceI recognition sequence
   Fragment containing a lacZ⍺ expression cassette flanked by BsaI sites
   Fragment containing an I-SceI recognition sequence
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRSFDuet1-His-TEV-OTC_Ub

   Plasmid

   #172134

   Purpose

   Scaffold design for studying ubiquitin interacting proteins by cryo-EM.

   Depositor

   Shang-Te Danny Hsu

   Article

   Chiu et al Biochemistry. 2021 Apr 13;60(14):1075-10

   Insert

   E. coli ornithine transcarbamylase (OTC), fused to human ubiquitin
   

   Use

   Tags

   6xHis-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Sept. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBAD24-rnc-sfGFP

   Plasmid

   #51560

   Purpose

   Gene encoding E. coli RNaseIII cloned as a N terminal translational fussion with superfolder GFP. Expression driven by arabinose (backbone is pBAD24).

   Depositor

   Francisco Malagon, Sankar Adhya

   Article

   Malagon et al RNA. 2013 Sep;19(9):1200-7. doi: 10.1261

   Insert

   rnc (rnc E. coli)
   

   Use

   Tags

   GLESTCRHASLAVLADERRFSA and superfolder GFP

   Expression

   Bacterial

   Mutation

   Contains additonal aa at the end of the sfGFP in …

   Promoter

   Available since

   March 26, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA5_NLS-Halo-eGFP-BioID

   Plasmid

   #218448

   Purpose

   expresses the NLS-Halo-eGFP-BioID fusion protein via stable Flp-In integration

   Depositor

   Thorsten Stafforst

   Article

   Hanswillemenke et al Nat Chem Biol. 2024 Jan 17. doi: 10.1038

   Insert

   NLS-Halo-eGFP-BirA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET41a iGluSnFR

   Plasmid

   #119828

   Purpose

   Production of recombinant iGluSnFR, a single-wavelength glutamate indicator, in E. coli

   Depositor

   Katalin Torok

   Article

   Helassa et al Proc Natl Acad Sci U S A. 2018 May 7. pi

   Insert

   iGluSnFR
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   April 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMJA089

   Plasmid

   #128518

   Purpose

   Expresses human Lysozyme in Pichia pastoris

   Depositor

   Marcos Alcocer

   Article

   Johnson et al J Mol Biol. 2005 Sep 30;352(4):823-36. d

   Insert

   Artificial gene for human lysozyme (LYZ Human)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   AOX1

   Available since

   July 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. asPink chromoprotein promoter-less

   Plasmid

   #118005

   Purpose

   For course in "Synthetic Biology: A Lab Manual"

   Depositor

   Anthony Forster

   Article

   Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

   Insert

   RBS, asPink
   

   Use

   Escherichia coli

   Tags

   Expression

   Mutation

   BioBrick sites removed

   Promoter

   Available since

   Aug. 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSO221 Phis-72 BirA GFP

   Plasmid

   #45100

   Depositor

   Steven Henikoff

   Article

   Steiner et al Genome Res. 2012 Apr;22(4):766-77. doi:

   Inserts

   BirA::GFP
   unc-119 (unc-119 Nematode)
   

   Use

   Tags

   GFP

   Expression

   Worm

   Mutation

   Promoter

   his-72 and unc-119

   Available since

   June 4, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAT-9218

    Plasmid

    #124225

    Purpose

    Bacterial SpCas9 expression

    Depositor

    Ervin Welker

    Article

    Talas et al Nucleic Acids Res. 2021 Jan 15. pii: 610

    Insert

    SpCas9
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    TetR/TetA

    Available since

    Nov. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pJL1-MBP-4xDQNAT

    Plasmid

    #128390

    Purpose

    In vitro expression of E. coli MBP with C terminal 4xDQNAT glycosylation sites

    Depositor

    Michael Jewett

    Article

    Stark et al Sci Adv. 2021 Feb 3;7(6). pii: 7/6/eabe9

    Insert

    Maltose binding protein
    

    Use

    Tags

    4xDQNAT glycosylation tag and 6xHis tag

    Expression

    Bacterial

    Mutation

    A302T- please see depositor comment

    Promoter

    T7

    Available since

    April 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pANTO5

    Plasmid

    #131106

    Purpose

    To generate an integrative plasmid carrying the TetR/Pip-OFF system, but not the integrase

    Depositor

    Riccardo Manganelli

    Article

    Boldrin et al Sci Rep. 2019 Apr 8;9(1):5783. doi: 10.1

    Insert

    tetR gene by pFRA61 (Boldrin et al 2010)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pJB332

    Plasmid

    #115949

    Purpose

    ORF insert for replicon MoClo

    Depositor

    Ron Weiss

    Article

    Wagner et al Nat Chem Biol. 2018 Nov;14(11):1043-1050

    Insert

    Lvl0 TetR-Gly9-DDX6 (contains SapI)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pTW122

    Plasmid

    #115962

    Purpose

    ORF insert for replicon MoClo

    Depositor

    Ron Weiss

    Article

    Wagner et al Nat Chem Biol. 2018 Nov;14(11):1043-1050

    Insert

    Lvl0 5'-2xTetApt-mVenus-PEST
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET41a iGluh

    Plasmid

    #119830

    Purpose

    Production of recombinant iGluSnFR E25A high affinity variant, a single-wavelength glutamate indicator, in E. coli

    Depositor

    Katalin Torok

    Article

    Helassa et al Proc Natl Acad Sci U S A. 2018 May 7. pi

    Insert

    iGluh
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    E25A

    Promoter

    T7

    Available since

    Aug. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. EcPPK1

    Plasmid

    #108850

    Purpose

    Expresses E. coli PPK1 in mammalian cell lines

    Depositor

    Michael Downey

    Article

    Bentley-DeSousa et al Cell Rep. 2018 Mar 27;22(13):3427-3439.

    Insert

    Polyphosphate Kinase 1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pXD70CT5-PxylR-CymR, Pveg(CuO)-LacZ

    Plasmid

    #191627

    Purpose

    E. coli - Eggerthella lenta shuttle plasmid (KanR), cumate-inducible promoter Pct5 (engineered from pCT5-bac2.0)-LacZ

    Depositor

    Emily Balskus

    Article

    Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

    Insert

    lacZ
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET22b-BirA-CPDSalI

    Plasmid

    #38258

    Depositor

    Aimee Shen, Matthew Bogyo

    Article

    Shen et al PLoS One. 2009 Dec 2;4(12):e8119.

    Insert

    birA (birA E. coli)
    

    Use

    Tags

    6xHis and Vibrio cholerae MARTX toxin cysteine pr…

    Expression

    Bacterial

    Mutation

    cloned from pGEX4T1-BirA

    Promoter

    T7

    Available since

    July 20, 2012

    Availability

    Academic Institutions and Nonprofits only

19. pSLS.416

    Plasmid

    #187207

    Purpose

    Expresses retron Eco1 ncRNA v35 (long a1/a2), and Cas1 + Cas2

    Depositor

    Seth Shipman

    Article

    Bhattarai-Kline et al Nature. 2022 Aug;608(7921):217-225. doi:

    Inserts

    Retron Eco1 ncRNA v35 (long a1/a2)
    Cas1 + Cas2
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7/Lac

    Available since

    Sept. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pOET3-N-TEV-Twin-Strep

    Plasmid

    #203323

    Purpose

    Generate the baculovirus to express C-terminal Tobacco Etch Virus (TEV) protease cleavable Twin-Strep-tagged protein (ENLYFQGS-WSHPQFEK-(GGGS)2-GGSA-WSHPQFEK) in insect cells

    Depositor

    Chiaki Murakami

    Article

    Furuta et al FEBS Open Bio. 2023 May 11. doi: 10.1002

    Type

    Empty backbone

    Use

    Tags

    TEV cleavable site (ENLYFQG) and Twin-strep-tag

    Expression

    Insect

    Mutation

    Promoter

    Available since

    July 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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