We narrowed to 40,933 results for: gats

1. pPpT4- TEF2prom-TIR1F74G-FLAG

   Plasmid

   #189727

   Purpose

   Enables expression of OsTIR1(F74G) from the TEF2 promoter, used to implement the AID2 system in Komagataella phaffii (original name pLB219)

   Depositor

   Anita Emmerstorfer-Augustin

   Article

   Lehmayer et al iScience. 2022 Aug 6;25(9):104888. doi:

   Insert

   OsTIR1
   

   Use

   Tags

   FLAG

   Expression

   Yeast

   Mutation

   Promoter

   TEF2

   Available since

   Sept. 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDONR221-OSF-TRIM5-21R

   Plasmid

   #79063

   Purpose

   Gateway entry vector for cloning and propagation in DH5alpha

   Depositor

   Wesley Sundquist

   Article

   Li et al Elife. 2016 Jun 2;5. pii: e16269. doi: 1

   Insert

   OSF-TRIM5-21R
   

   Use

   Tags

   OneSTrEP-FLAG

   Expression

   Insect

   Mutation

   Promoter

   T7

   Available since

   Jan. 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSLC-239

   Plasmid

   #73193

   Purpose

   Template plasmid which encodes kanamycin resistance gene for positive selection and toxin gene (yhaV) under the control of rhamnose induceable promoter (PrhaB) for negative selection.

   Depositor

   Swaine Chen

   Article

   Khetrapal et al Nucleic Acids Res. 2015 Jul 27;43(13):e8

   Insert

   PrhaB-yhaV (yhaV )
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PrhaB

   Available since

   July 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCellFree_G08

   Plasmid

   #67142

   Purpose

   Gateway destination vector for cell-free expression with C-term mCherry myc-tag tag

   Depositor

   Kirill Alexandrov

   Article

   Gagoski et al J Biotechnol. 2015 Feb 10;195:1-7. doi:

   Type

   Empty backbone

   Use

   Cell-free expression

   Tags

   His-MYC and mCherry

   Expression

   Mutation

   Promoter

   Available since

   Aug. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pENTR221-Firefly

   Plasmid

   #79495

   Purpose

   MultiSite Gateway entry clones, second fragment (attL5, attL2) Firefly Luciferase CDS

   Depositor

   Peter Jon Nelson

   Article

   Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

   Insert

   Firefly Luciferase
   

   Use

   Multisite gateway entry clone

   Tags

   Expression

   Mutation

   Promoter

   no Promoter

   Available since

   May 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pENTR221-Renilla

   Plasmid

   #79496

   Purpose

   MultiSite Gateway entry clones, second fragment (attL5, attL2) Renilla Luciferase CDS

   Depositor

   Peter Jon Nelson

   Article

   Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

   Insert

   Renilla Luciferase
   

   Use

   Multisite gateway entry clone

   Tags

   Expression

   Mutation

   Promoter

   no Promoter

   Available since

   May 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pU6_(GLuc)_TOP1

   Plasmid

   #68423

   Purpose

   Transient expression of the "TOP1" construct, targeting the GLuc reporter, in mammalian cells. U6 promoter

   Depositor

   John Rinn

   Article

   Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

   Insert

   TOP1 construct
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   human U6

   Available since

   Oct. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. IO001: pMVP (R4-R3) eGFP

   Plasmid

   #121730

   Purpose

   pMVP R4-R3 entry plasmid, contains eGFP for 3- or 4-component MultiSite Gateway Pro assembly

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Insert

   eGFP
   

   Use

   Synthetic Biology; Pmvp gateway entry plasmid

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. IG301: pMVP (L1-R5) CMV promoter

   Plasmid

   #121692

   Purpose

   pMVP L1-R5 entry plasmid, contains CMV promoter for 4-component MultiSite Gateway Pro assembly

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Insert

   CMV promoter
   

   Use

   Synthetic Biology; Pmvp gateway entry plasmid

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. p3E-ML1N*2(7Q)

    Plasmid

    #67679

    Purpose

    Multisite gateway entry clone for adding C-terminal fusions of p3E-ML1N*2(7Q)

    Depositor

    Rob Parton

    Article

    Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

    Insert

    ML1N*2(7Q)
    

    Use

    Gateway multisite 3' entry clone

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCFJ1001

    Plasmid

    #44476

    Depositor

    Erik Jorgensen

    Article

    Frokjaer-Jensen et al Nat Methods. 2014 May;11(5):529-34. doi:

    Insert

    Minimal Mos1, unc-119, peel-1, [4-3] Gateway
    

    Use

    Gateway destination vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 30, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. p667-UBC-DYRK2-FullLength-V5-miniTurbo_IDG-K

    Plasmid

    #198010

    Purpose

    Gateway destination clone of DYRK2 (human) tagged with C-terminal V5-miniTurbo for generating protein-proximity networks using proximity-dependent biotinylation proteomics

    Depositor

    Ben Major

    Insert

    DYRK2-V5-miniTurbo (DYRK2 Human)
    

    Use

    Lentiviral; Gateway destination

    Tags

    V5-miniTurbo

    Expression

    Mammalian

    Mutation

    Promoter

    Ubiquitin

    Available since

    April 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pFA-sbtA

    Plasmid

    #133011

    Purpose

    pFA carrying a Synechococcus elongatus PCC 7942 sbtA gene (Uniprot: Q6UP27)

    Depositor

    David Savage

    Article

    Desmarais et al Nat Microbiol. 2019 Aug 12. pii: 10.1038

    Insert

    SbtA
    

    Use

    Tags

    6xhis

    Expression

    Bacterial

    Mutation

    Promoter

    tetR

    Available since

    Nov. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pENTR221-sGFP

    Plasmid

    #79508

    Purpose

    MultiSite Gateway entry clones, second fragment (attL5, attL2) Super GFP CDS

    Depositor

    Peter Jon Nelson

    Article

    Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

    Insert

    superGFP
    

    Use

    Multisite gateway entry clone

    Tags

    6xHis

    Expression

    Mutation

    Promoter

    no Promoter

    Available since

    May 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. IBMc233

    Plasmid

    #161939

    Purpose

    Carries Golden Gate substitution insert for IBM with M86 intein. PPhlF as C-lobe promoter

    Depositor

    Baojun Wang

    Article

    Ho et al Nat Commun. 2021 Apr 13;12(1):2200. doi:

    Insert

    pSB1A3-BbsI-M86N-T-cat-PPhlF-RiboJ-B32-M86C-SapI
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PPhlF

    Available since

    March 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCFJ1002

    Plasmid

    #44477

    Depositor

    Erik Jorgensen

    Article

    Frokjaer-Jensen et al Nat Methods. 2014 May;11(5):529-34. doi:

    Insert

    Minimal Mos1, NeoR, peel-1, [4-3] Gateway
    

    Use

    Gateway destination vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 31, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDEST(frt)(ATG_V5-APEX2_noSTOP)

    Plasmid

    #168207

    Purpose

    Destination vector for GATEWAY cloning, N-terminal V5-APEX2, Tet operator in the CMV promoter (Tet ON), FRT sequence for T-Rex stable expression, Hygromycin selection

    Depositor

    Markus Hafner

    Article

    Benhalevy et al Nat Methods. 2018 Dec;15(12):1074-1082.

    Type

    Empty backbone

    Use

    Unspecified; Pdest gateway

    Tags

    V5 APEX2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. p663-UBC-miniTurbo-V5-CDK16_IDG-K

    Plasmid

    #158173

    Purpose

    Gateway destination clone of CDK16 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

    Depositor

    Ben Major

    Insert

    miniTurbo-V5-CDK16 (CDK16 Human)
    

    Use

    Lentiviral; Gateway destination

    Tags

    miniTurbo-V5

    Expression

    Mammalian

    Mutation

    Promoter

    Ubiquitin

    Available since

    Aug. 31, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. p3E-FRB-HA no-pA

    Plasmid

    #82594

    Purpose

    3' entry vector containing HA epitope fused to C-terminus of TOR FKBP and rapamycin binding (FRB) domain without pA for rapamycin/rapalog induced dimerization

    Depositor

    Kryn Stankunas

    Article

    Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

    Insert

    FRB-HA (no pA)
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    None

    Available since

    Dec. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. C455-E01: attB4-stuffer-attB5

    Plasmid

    #162983

    Purpose

    Gateway attB4-attB5 entry clone containing a non-transcribed/translated region for use in generating negative control vectors

    Depositor

    Dominic Esposito

    Article

    Wall et al Methods Mol Biol. 2014;1116:193-208. doi

    Insert

    stuffer fragment for vector controls
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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