We narrowed to 10,632 results for: t7

1. pDiySpike1

   Plasmid

   #136948

   Purpose

   Plasmid for the In Vitro transcription of DiySpike1

   Depositor

   Rickard Sandberg

   Article

   Hagemann-Jensen et al Nat Biotechnol. 2020 Jun;38(6):708-714.

   Insert

   DiySpike1
   

   Use

   Synthetic Biology

   Promoter

   T7

   Available Since

   April 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRSETB-ro1GFP

   Plasmid

   #82365

   Purpose

   Inducible expression of redox sensitive GFP in bacteria

   Depositor

   S. James Remington

   Article

   Hanson et al J Biol Chem. 2004 Mar 26;279(13):13044-5

   Insert

   ro1GFP
   

   Tags

   6xHIS

   Expression

   Bacterial

   Mutation

   C48S/Q80R/S147C/Q204C

   Promoter

   T7

   Available Since

   Sept. 19, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET/LEGH

   Plasmid

   #108226

   Purpose

   Bacterial expression of EGFP with N-terminal lanthanide binding tag and C-terminal His6 tag

   Depositor

   Didier Picard

   Article

   Bhattacharya et al Sci Rep. 2018 Feb 12;8(1):2801. doi: 10.

   Insert

   LBT-EGFP-His6
   

   Expression

   Bacterial

   Promoter

   T7, lacO

   Available Since

   April 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. TAL2384

   Plasmid

   #36793

   Depositor

   Keith Joung

   Article

   Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

   Insert

   BRCA1-TALEN_Left (Brca1 Human)
   

   Use

   Mammalian, t7 for in vitro mrna synthesis

   Tags

   3X Flag and WT FOKI

   Promoter

   CMV

   Available Since

   June 26, 2012

   Availability

   Academic Institutions and Nonprofits only

5. pRC01

   Plasmid

   #114680

   Purpose

   sfGFP.Strands 1-9

   Depositor

   Matthew Good

   Article

   Caldwell et al Biochemistry. 2018 May 8;57(18):2590-259

   Insert

   sfGFP Strands 1-9
   

   Tags

   GST

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Sept. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCR-Blunt_hNANOG

   Plasmid

   #112639

   Purpose

   Plasmid for PCR amplification of hNANOG DNA template for in vitro transcription

   Depositor

   Ganna Bilousova

   Article

   McGrath et al J Vis Exp. 2018 Nov 26;(141). doi: 10.37

   Insert

   hNANOG (NANOG Human)
   

   Use

   Pcr template

   Promoter

   T7

   Available Since

   Nov. 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. Drp1(A395D)-StrepII

   Plasmid

   #174430

   Purpose

   Expresses human Drp1 Isoform 3 with A395D mutation in bacteria

   Depositor

   Thomas Pucadyil

   Article

   Kamerkar et al Nat Commun. 2018 Dec 7;9(1):5239. doi: 1

   Insert

   Drp1
   

   Tags

   StrepII

   Expression

   Bacterial

   Mutation

   A395D

   Promoter

   T7

   Available Since

   Sept. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET15b+PCNA1 P186C

   Plasmid

   #66173

   Purpose

   Expresses the P186C mutant of Sulfolobus solfataricus PCNA1

   Depositor

   Teruyuki Nagamune

   Article

   Hirakawa et al Biotechnol Bioeng. 2013 Jul;110(7):1858-

   Insert

   DNA polymerase sliding clamp 1
   

   Tags

   His6 tag

   Expression

   Bacterial

   Mutation

   P186C

   Promoter

   T7 promoter

   Available Since

   June 29, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. TALEN1259

   Plasmid

   #32284

   Depositor

   Keith Joung

   Article

   Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

   Insert

   TAL array targeting gria3a (gria3a Zebrafish)
   

   Use

   T7

   Tags

   3X Flag and WT FOKI

   Expression

   Mammalian

   Available Since

   Oct. 27, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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10. hPRL3_1-169

    Plasmid

    #209796

    Purpose

    Bacterial expression of human PRL-3 without farnesylation motif

    Depositor

    Kalle Gehring

    Article

    Kozlov et al J Biol Chem. 2004 Mar 19. 279(12):11882-

    Insert

    PRL-3 (PTP4A3 Human)
    

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    aa 1-169 only

    Promoter

    T7

    Available Since

    Nov. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. TALEN1258

    Plasmid

    #32281

    Depositor

    Keith Joung

    Article

    Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

    Insert

    TAL array targeting gria3a (gria3a Zebrafish)
    

    Use

    T7

    Tags

    3X Flag and FOKI WT

    Expression

    Mammalian

    Available Since

    Oct. 27, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDM_noro_S2_lacZA

    Plasmid

    #118816

    Purpose

    T7 RNAP-driven expression of norovirus sense orientation toehold switches (S2) with a lacZ alpha subunit reporter.

    Depositor

    Alexander Green

    Article

    Ma et al Synth Biol (Oxf). 2018;3(1):ysy018. doi:

    Insert

    Norovirus toehold switch S2
    

    Expression

    Bacterial

    Available Since

    Jan. 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pJK667

    Plasmid

    #71690

    Purpose

    Produces E. coli acetate kinase with an N-terminal His6 tag (H6AckA)

    Depositor

    T. J. Kappock

    Article

    Murphy et al Front Chem. 2016 May 23;4:23. doi: 10.33

    Insert

    acetate kinase (ackA Escherichia coli)
    

    Tags

    His6

    Expression

    Bacterial

    Promoter

    T7 promoter

    Available Since

    June 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET20b-GpNLuc-6xHis

    Plasmid

    #135932

    Purpose

    Expresses the GpNLuc LumiFluor in bacterial cells.

    Depositor

    Antonio Amelio

    Article

    Parag-Sharma et al ACS Synth Biol. 2019 Dec 13. doi: 10.102

    Insert

    GpNLuc LumiFluor
    

    Tags

    6xHis

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMCSG26(gfcD)

    Plasmid

    #176198

    Purpose

    gfcD protein expression

    Depositor

    Mark Saper

    Article

    Larson et al PLoS One. 2021 Nov 15;16(11):e0259900. d

    Insert

    gfcD
    

    Tags

    10xHis

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCellFree_G03 PDGFRB

    Plasmid

    #67130

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    PDGFRB (PDGFRB Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Promoter

    T7

    Available Since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

17. pGBW-m4092686

    Plasmid

    #153895

    Purpose

    Bacterial expression plasmid for fragment of SARS-CoV-2 orf1ab 3pUTR to viral genome 3pUTR

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    fragment of SARS-CoV-2 orf1ab 3pUTR to viral genome 3pUTR (ORF1ab Severe acute respiratory syndrome coronavirus 2)
    

    Expression

    Bacterial

    Mutation

    Nearly native encoding;inactivating mutations in …

    Promoter

    Promoter | t7 consensus from NEB, G is +1

    Available Since

    June 12, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. HIV-1 integrase F185H/C280S in pET15b

    Plasmid

    #61672

    Purpose

    HIV-1 integrase F185H/C280S in pET-15b

    Depositor

    Robert Craigie

    Article

    Jenkins et al J Biol Chem. 1996 Mar 29;271(13):7712-8.

    Insert

    HIV-1 integrase F185H/C280S
    

    Use

    Synthetic Biology

    Tags

    His

    Promoter

    T7 promotor

    Available Since

    Feb. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

19. pDM_noro_S6_lacZA

    Plasmid

    #118817

    Purpose

    T7 RNAP-driven expression of norovirus sense orientation toehold switches (S6) with a lacZ alpha subunit reporter.

    Depositor

    Alexander Green

    Article

    Ma et al Synth Biol (Oxf). 2018;3(1):ysy018. doi:

    Insert

    Norovirus toehold switch S6
    

    Expression

    Bacterial

    Available Since

    Jan. 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCellFree_G03 NFX1

    Plasmid

    #67131

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    NFX1 (NFX1 Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Promoter

    T7

    Available Since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only