We narrowed to 29,593 results for: REP

1. px335 Slc1a3 nickase 1

   Plasmid

   #129410

   Purpose

   Cas9n (nickase) with sgRNA directed to the terminal exon of mouse Slc1a3 gene

   Depositor

   Walker Jackson

   Article

   Kaczmarczyk et al Sci Rep. 2021 Mar 8;11(1):5412. doi: 10.

   Insert

   sgRNA directing Cas9n to the proximity of Slc1a3 STOP codon (Slc1a3 Mouse)
   

   Use

   CRISPR and Mouse Targeting

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. px335 Slc1a3 nickase 2

   Plasmid

   #129411

   Purpose

   Cas9n (nickase) with sgRNA directed to the terminal exon of mouse Slc1a3 gene

   Depositor

   Walker Jackson

   Article

   Kaczmarczyk et al Sci Rep. 2021 Mar 8;11(1):5412. doi: 10.

   Insert

   sgRNA targeting Slc1a3 (Slc1a3 Mouse)
   

   Use

   CRISPR and Mouse Targeting

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLV-Hygro-EF1A-RG-Linker-eGFP

   Plasmid

   #176071

   Purpose

   EGFP fused to the C-terminus of a RG domain & a hygromycin resistance cassette

   Depositor

   Robert Sobol

   Article

   Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

   Insert

   RG
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   EF1A

   Available since

   Feb. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLV-Hygro-EF1A-KR-Linker-eGFP

   Plasmid

   #176066

   Purpose

   EGFP fused to the C-terminus of a KR domain & a hygromycin resistance cassette

   Depositor

   Robert Sobol

   Article

   Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

   Insert

   KR
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   EF1A

   Available since

   Jan. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCDNA4 3xFLAG Halo G-Linker nsp1

   Plasmid

   #175426

   Purpose

   Expresses G-linker CoV2 nsp1 in mammalian cells. This contains a 3XFLAG Halo tag fusion to nsp1.

   Depositor

   Britt Glaunsinger

   Article

   Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

   Insert

   G-linker nsp1
   

   Use

   Tags

   3XFLAG Halo

   Expression

   Mammalian

   Mutation

   G-linker in place of the RNase destabilization do…

   Promoter

   CMV

   Available since

   Oct. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCDNA4 3xFLAG Halo R119A K120A nsp1

   Plasmid

   #175428

   Purpose

   Expresses R119A K120A CoV2 nsp1 in mammalian cells. This contains a 3XFLAG Halo tag fusion to nsp1.

   Depositor

   Britt Glaunsinger

   Article

   Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

   Insert

   nsp1 R119A K120A
   

   Use

   Tags

   3XFLAG Halo

   Expression

   Mammalian

   Mutation

   N-terminal mutants R119A K120A

   Promoter

   CMV

   Available since

   Oct. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCDNA4 3xFLAG Halo E55A E57A K58A nsp1

   Plasmid

   #175427

   Purpose

   Expresses E55A E57A K58A CoV2 nsp1 in mammalian cells. This contains a 3XFLAG Halo tag fusion to nsp1.

   Depositor

   Britt Glaunsinger

   Article

   Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

   Insert

   nsp1 E55A E57A K58A
   

   Use

   Tags

   3XFLAG Halo

   Expression

   Mammalian

   Mutation

   N-terminal mutants E55A E57A K58A

   Promoter

   CMV

   Available since

   Oct. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCDNA4 3xFLAG Halo E36A E37A nsp1

   Plasmid

   #175429

   Purpose

   Expresses E36A E37A CoV2 nsp1 in mammalian cells. This contains a 3XFLAG Halo tag fusion to nsp1.

   Depositor

   Britt Glaunsinger

   Article

   Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

   Insert

   nsp1 E36A E37A
   

   Use

   Tags

   3XFLAG Halo

   Expression

   Mammalian

   Mutation

   N-terminal mutants E36A E37A

   Promoter

   CMV

   Available since

   Oct. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCAG-Bmpr2ΔEC gRNA resistant

   Plasmid

   #163413

   Purpose

   Expresses BMPR-2ΔEC resistant to the gRNAs(#163388-90) in mammalian cells

   Depositor

   Takeshi Imai

   Article

   Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

   Insert

   Bmpr2 (Bmpr2 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   The extracellular domain (27-150 aa) is deleted. …

   Promoter

   Available since

   Aug. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCAG-Bmpr2ΔTail gRNA resistant

    Plasmid

    #163412

    Purpose

    Expresses BMPR-2ΔTail resistant to the gRNAs(#163388-90) in mammalian cells

    Depositor

    Takeshi Imai

    Article

    Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

    Insert

    Bmpr2 (Bmpr2 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    The C-terminal tail domain (530-1038 aa) is delet…

    Promoter

    Available since

    Aug. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pQlinkG2-PTPIP51(236-470)

    Plasmid

    #170534

    Purpose

    Expresses Cleavable GST-tagged PTPIP51 TPR domain (236-470) in E. Coli

    Depositor

    Byung Il Lee

    Article

    Yeo et al EMBO Rep. 2021 Jun 4;22(6):e51323. doi:

    Insert

    Protein tyrosine phosphatase interacting protein 51 (RMDN3 Human)
    

    Use

    Tags

    GST tag

    Expression

    Bacterial

    Mutation

    Promoter

    T5 promoter

    Available since

    Aug. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pFASTbac-HisGST-PTPIP51(36-470)

    Plasmid

    #170531

    Purpose

    Expresses Cleavable HisGST-tagged PTPIP51 (36-470) in Sf9

    Depositor

    Byung Il Lee

    Article

    Yeo et al EMBO Rep. 2021 Jun 4;22(6):e51323. doi:

    Insert

    Protein tyrosine phosphatase interacting protein 51 (RMDN3 )
    

    Use

    Tags

    GST tag and His tag

    Expression

    Insect

    Mutation

    Promoter

    Polyhedrin

    Available since

    Aug. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pQlinkH-PTPIP51(236-470)

    Plasmid

    #170530

    Purpose

    Expresses Cleavable His-tagged PTPIP51 TPR domain (236-470) in E. Coli

    Depositor

    Byung Il Lee

    Article

    Yeo et al EMBO Rep. 2021 Jun 4;22(6):e51323. doi:

    Insert

    Protein tyrosine phosphatase interacting protein 51 (236-470) (RMDN3 Human)
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    Promoter

    T5 promoter

    Available since

    July 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. YF

    Plasmid

    #171459

    Purpose

    Expresses YF, a FiCE of iCMM, in mammalian cells

    Depositor

    Takafumi Miyamoto

    Article

    Miyamoto et al Cell Rep Methods. 2021 Jul 22;1(4):10005

    Insert

    EYFP-FKBP12
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. mYF

    Plasmid

    #171460

    Purpose

    Expresses mYF, a CiCE of NiCMM, in mammalian cells

    Depositor

    Takafumi Miyamoto

    Article

    Miyamoto et al Cell Rep Methods. 2021 Jul 22;1(4):10005

    Insert

    EYFP(A207K)-FKBP12
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. mActZ-FmCh

    Plasmid

    #171465

    Purpose

    Expresses mActZ-FmCh, a FiCE of iCMM, in mammalian cells

    Depositor

    Takafumi Miyamoto

    Article

    Miyamoto et al Cell Rep Methods. 2021 Jul 22;1(4):10005

    Insert

    ActA(30-262 aa)-Zyxin(2-380 aa)-FKBP12-mCherry
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET-DEST42 -SSRP1

    Plasmid

    #170439

    Purpose

    Express drosophila SSRP1 in E.coli expression system. Generated from pDONR-SSRP1 by Gateway system. Used for custom antibody purification.

    Depositor

    Joan Conaway

    Article

    Tettey et al Cell Rep. 2019 Jun 25;27(13):3770-3779.e

    Insert

    ssrp1 (Ssrp Fly)
    

    Use

    Tags

    6xHis and V5

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSUMO ACD11

    Plasmid

    #170738

    Purpose

    The plasmids contain inserts (ORFs) for expressing various members of the GlycoLipid Transfer Protein (GLTP) superfamily.

    Depositor

    Rhoderick Brown

    Article

    Simanshu et al Cell Rep. 2014 Jan 30;6(2):388-99. doi:

    Insert

    acd11 (ACD11 Mustard Weed)
    

    Use

    Tags

    SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. PWPXLD-EGFR-P667A

    Plasmid

    #133750

    Purpose

    EGFR with mutation at proline 667 converted to alanine and tagged with HA cloned into PWPXLD plasmid

    Depositor

    Chay Kuo

    Article

    Abdi et al Cell Rep. 2019 Aug 20;28(8):2012-2022.e4

    Insert

    Mutant P667A EGFR-HA (EGFR Human)
    

    Use

    Lentiviral

    Tags

    HA tag

    Expression

    Mammalian

    Mutation

    proline 667 converted to alanine (Please see depo…

    Promoter

    EF1-alpha

    Available since

    May 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pEGFP_∆R13–DD-talin1 (1-2299)

    Plasmid

    #166125

    Purpose

    For expression of mouse talin-1 (residues 1-2299) with C-terminal deletion of dimerization domain and R13 (∆R13-DD) in mammalian cells. Contains C-terminal EGFP tag.

    Depositor

    Vesa Hytönen

    Article

    Azizi et al Sci Rep. 2021 Jan 11;11(1):347. doi: 10.

    Insert

    ∆R13–DD-Talin-1 (Tln1 Mouse)
    

    Use

    Cmv promoter

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    residues 1-2299, deletion of dimerization domain …

    Promoter

    Available since

    April 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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