Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

We narrowed to 13,867 results for: cas9

Showing: 10161 - 10200 of 13867 results

  1. pZNF266.2.0-gDNA

    Plasmid
    #112402
    Purpose
    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor ZNF266
    Depositor
    Kevin White
    Insert
    ZNF266 (ZNF266 Human)
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available Since
    Dec. 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    112402-plasmid-map-sequence-id-230257

    Enlarge

  2. pZNF507.1.0-gDNA

    Plasmid
    #112401
    Purpose
    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor ZNF507
    Depositor
    Kevin White
    Insert
    ZNF507 (ZNF507 Human)
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available Since
    Dec. 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    112401-plasmid-map-sequence-id-230255

    Enlarge

  3. pZSCAN32.1.0-gDNA

    Plasmid
    #112400
    Purpose
    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor ZNF434
    Depositor
    Kevin White
    Insert
    ZNF434 (ZSCAN32 Human)
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available Since
    Dec. 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    112400-plasmid-map-sequence-id-230252

    Enlarge

  4. pZNF165.2.0-gDNA

    Plasmid
    #112398
    Purpose
    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor ZNF165
    Depositor
    Kevin White
    Insert
    ZNF165 (ZNF165 Human)
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available Since
    Dec. 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    112398-plasmid-map-sequence-id-230245

    Enlarge

  5. pZNF174.1.0-gDNA

    Plasmid
    #112397
    Purpose
    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor ZNF174
    Depositor
    Kevin White
    Insert
    ZNF174 (ZNF174 Human)
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available Since
    Dec. 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    112397-plasmid-map-sequence-id-230244

    Enlarge

  6. pZNF764.1.0-gDNA

    Plasmid
    #112394
    Purpose
    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor ZNF764
    Depositor
    Kevin White
    Insert
    ZNF764 (ZNF764 Human)
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available Since
    Dec. 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    112394-plasmid-map-sequence-id-230241

    Enlarge

  7. pZNF57.1.0-gDNA

    Plasmid
    #112392
    Purpose
    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor ZNF57
    Depositor
    Kevin White
    Insert
    ZNF57 (ZNF57 Human)
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available Since
    Dec. 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    112392-plasmid-map-sequence-id-230238

    Enlarge

  8. pZNF257.1.0-gDNA

    Plasmid
    #112390
    Purpose
    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor ZNF257
    Depositor
    Kevin White
    Insert
    ZNF257 (ZNF257 Human)
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available Since
    Dec. 4, 2018
    Availability
    Academic Institutions and Nonprofits only
    112390-plasmid-map-sequence-id-229882

    Enlarge

  10. gTBEv4

    Plasmid
    #220620
    Purpose
    vector for encoding an engineered glycosylase-based thymine (T) base editor (gTBEv4) driven by EF1a promoter, guide RNAs compatible with SpCas9 driven by hU6, mCherry driven by CBh promoter
    Depositor
    Huawei Tong
    Article
    Tong et al Nat Commun. 2024 Jun 8;15(1):4897. doi:
    Insert
    nSpCas9(Nterm)-XTEN-hUNG2-XTEN-Cas9(Cterm)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    hUNG2(del1-88;Y156A,A214T,Q259A,Y284D), SpCas9(D1…
    Promoter
    hU6, EF1a
    Available Since
    June 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    220620-plasmid-map-sequence-id-436970

    Enlarge

  11. AICSDP-15:ACTB-mEGFP

    Plasmid
    #87425
    Purpose
    Homology arms and linker-mEGFP sequence for N-terminus tagging of human ACTB
    Depositor
    Allen Institute for Cell Science
    Article
    Roberts et al Mol Biol Cell. 2017 Oct 15;28(21):2854-2
    Insert
    ACTB Homology Arms with mEGFP-linker (ACTB Human)
    Use
    CRISPR; Donor template
    Tags
    mEGFP-linker
    Expression
    Mammalian
    Mutation
    homology arms contain point mutations to disrupt …
    Promoter
    Available Since
    March 21, 2017
    Availability
    Industry, Academic Institutions, and Nonprofits
    87425-plasmid-map-sequence-id-174163

    Enlarge

  12. pUC19-U6-BsmBI_epegRNA_entry-mpknot_(LM1140)

    Plasmid
    #228253
    Purpose
    pU6 entry plamid for cloning prime editor mpknot epegRNAs (requires BsmBI or Esp3I digest and ligation of duplexed oligos)
    Depositor
    Benjamin Kleinstiver
    Article
    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586
    Insert
    mpknot entry plasmid backbone for epegRNA expression via human U6 promoter
    Use
    Tags
    Expression
    Mammalian
    Mutation
    n/a
    Promoter
    U6
    Available Since
    April 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    228253-plasmid-map-sequence-id-451024

    Enlarge

  13. AAV:ITR-U6-sgRNA(backbone)-pCBh-Cre-WPRE-hGHpA-ITR

    Plasmid
    #60229
    Purpose
    Expresses Cre recombinase from the Cbh promoter and one U6-driven sgRNA. AAV backbone with SapI spacer for sgRNA cloning.
    Depositor
    Feng Zhang
    Article
    Platt et al Cell. 2014 Sep 24. pii: S0092-8674(14)01
    Inserts
    sgRNA
    Cre recombinase
    Use
    AAV, CRISPR, Cre/Lox, and Mouse Targeting
    Tags
    Cre-HA
    Expression
    Mammalian
    Mutation
    Promoter
    CBh and U6
    Available Since
    Oct. 9, 2014
    Availability
    Academic Institutions and Nonprofits only
    60229-plasmid-map-sequence-id-419029

    Enlarge

  14. gTBEv5

    Plasmid
    #220621
    Purpose
    vector for encoding an engineered glycosylase-based thymine (T) base editor (gTBEv5) driven by EF1a promoter, guide RNAs compatible with SpCas9 driven by hU6, mCherry driven by CBh promoter
    Depositor
    Huawei Tong
    Article
    Tong et al Nat Commun. 2024 Jun 8;15(1):4897. doi:
    Insert
    nSpCas9(Nterm)-XTEN-hUNG2-XTEN-Cas9(Cterm)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    hUNG2(del1-88;Y156A,A214T,Q259A,Y284D), SpCas9(D1…
    Promoter
    hU6, EF1a
    Available Since
    June 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    220621-plasmid-map-sequence-id-436953

    Enlarge

  15. pcDNA3-Flag::UbvG08 I44A, deltaGG

    Plasmid
    #74939
    Purpose
    Ubiquitin variant that binds to and occludes the ligand binding site of the 53BP1 Tudor domain (i53). Blocks 53BP1 from accumulating at sites of DNA damage. Potent selective inhibitor of 53BP1
    Depositor
    Daniel Durocher
    Article
    Canny et al Nat Biotechnol. 2018 Jan;36(1):95-102. d
    Insert
    Ubiquitin
    Use
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    UbvG08 with I44A mutation and no terminal Glycines
    Promoter
    CMV
    Available Since
    June 30, 2016
    Availability
    Industry, Academic Institutions, and Nonprofits
    74939-plasmid-map-sequence-id-151347

    Enlarge

  17. gCBEv3

    Plasmid
    #220618
    Purpose
    vector for encoding an engineered glycosylase-based cytosine (C) base editor (gCBEv3) driven by EF1a promoter, guide RNAs compatible with SpG driven by hU6, mCherry driven by CBh promoter
    Depositor
    Huawei Tong
    Article
    Tong et al Nat Commun. 2024 Jun 8;15(1):4897. doi:
    Insert
    nSpCas9(Nterm)-XTEN-hUNG2-XTEN-Cas9(Cterm)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    hUNG2(del1-88;N213D,K184A,A214V), SpCas9(D10A)
    Promoter
    hU6, EF1a
    Available Since
    June 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    220618-plasmid-map-sequence-id-436973

    Enlarge

  23. pUC19-U6-[BsmBI_pegRNA_entry]-tevopreQ1-term (LM1138)

    Plasmid
    #223137
    Purpose
    pU6 entry plamid for cloning prime editor tevopreQ1 epegRNAs (requires BsmBI or Esp3I digest and ligation of duplexed oligos)
    Depositor
    Benjamin Kleinstiver
    Article
    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586
    Insert
    etevopreQ1 entry plasmid backbone for epegRNA expression via human U6 promoter
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available Since
    April 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    223137-plasmid-map-sequence-id-447707

    Enlarge

  26. pYEE

    Plasmid
    #159750
    Purpose
    Egg cell-specific promoter-controlled expression of 3×FLAG-NLS-zCas9-NLS. Contains gRNA scaffold for insertion of target sequence (U6-26 promoter). Selection of transgenic seeds by YFP fluoresce.
    Depositor
    Wolfgang Lukowitz
    Article
    Angulo et al bioRxiv
    Insert
    gRNA scaffold
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available Since
    Oct. 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    159750-plasmid-map-sequence-id-313819

    Enlarge

  29. pMX-HA-UbvG08-IRES-GFP

    Plasmid
    #75030
    Purpose
    Retroviral vector with ubiquitin variant that binds to and occludes the ligand binding site of the 53BP1 Tudor domain (i53)
    Depositor
    Daniel Durocher
    Article
    Canny et al Nat Biotechnol. 2018 Jan;36(1):95-102. d
    Insert
    Ubiquitin
    Use
    Retroviral
    Tags
    HA and IRES-eGFP
    Expression
    Mutation
    UbvG08 with I44A mutation and no terminal Glycines
    Promoter
    Available Since
    June 30, 2016
    Availability
    Industry, Academic Institutions, and Nonprofits
    75030-plasmid-map-sequence-id-436413

    Enlarge

  31. pAAV-i53-DM

    Plasmid
    #92171
    Purpose
    recombinant adeno-associated viral plasmid for delivery and expression of ubiquitin variant that has reverted mutations L69P and V70L (i53-DM)
    Depositor
    Daniel Durocher
    Article
    Canny et al Nat Biotechnol. 2018 Jan;36(1):95-102. d
    Insert
    i53-DM
    Use
    AAV
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    UbvG08 with I44A, P69L, and L70V mutations and no…
    Promoter
    CMV
    Available Since
    July 13, 2017
    Availability
    Industry, Academic Institutions, and Nonprofits
    92171-plasmid-map-sequence-id-182616

    Enlarge

  34. pTEE

    Plasmid
    #159748
    Purpose
    Egg cell-specific promoter-controlled expression of 3×FLAG-NLS-zCas9-NLS. Contains gRNA scaffold for insertion of target sequence (U6-26 promoter). Selection of transgenic seeds by mTomato fluoresce.
    Depositor
    Wolfgang Lukowitz
    Article
    Angulo et al bioRxiv
    Insert
    gRNA scaffold
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available Since
    Oct. 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    159748-plasmid-map-sequence-id-313816

    Enlarge

  37. PX459-gR2A_Hinge

    Plasmid
    #124811
    Purpose
    Vector for expression Cas9 with gRNA specific for rat IgG2a heavy chain locus. Use in combination with pHybr_r2a>Fab-srt-his plasmids to convert expression of hybridomas to Fab' fragments.
    Depositor
    Ferenc Scheeren, Martijn Verdoes
    Article
    van der Schoot et al Sci Adv. 2019 Aug 28;5(8):eaaw1822. doi:
    Insert
    Cas9 – gRNA_R2A_hinge
    Use
    CRISPR
    Tags
    3XFLAG and GFP
    Expression
    Bacterial and Mammalian
    Mutation
    R166H in PuroR (please see depositors comment bel…
    Promoter
    pUC
    Available Since
    Sept. 9, 2019
    Availability
    Academic Institutions and Nonprofits only
    124811-plasmid-map-sequence-id-243700

    Enlarge

  40. pCEE

    Plasmid
    #159746
    Purpose
    Egg cell-specific promoter-controlled expression of 3×FLAG-NLS-zCas9-NLS. Contains gRNA scaffold for insertion of target sequence (U6-26 promoter). Selection of transgenic seeds by CFP fluoresce.
    Depositor
    Wolfgang Lukowitz
    Article
    Angulo et al bioRxiv
    Insert
    gRNA scaffold
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available Since
    Oct. 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    159746-plasmid-map-sequence-id-313813

    Enlarge

Showing: 10161 - 10200 of 13867 results