We narrowed to 10,550 results for: aav

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pAAV-CAG-EGFP-P2A-mCherry

Plasmid

#229604

Purpose

Expresses EGFP and mCherry in mammalian cells

Depositor

Kiryl Piatkevich

Publication

Zhang et al Nat Methods. 2024 Apr;21(4):657-665. doi

Insert

EGFP

Use

AAV

Expression

Mammalian

Available since

July 17, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn T21R-F8.2 (S54L+T127A)-T2A-mCherry

Plasmid

#225590

Purpose

AAV transgene plasmid with hSyn promoter for expression of TRIM21 RING-F8.2(S54L+T127A) anti-tau degrader with a self-cleaving T2A-mCherry fluorescent expression reporter

Depositor

William McEwan

Publication

Benn et al Science. 2024 Aug 30;385(6712):1009-1016

Insert

T21R-F8.2 (S54L+T127A)-T2A-mCherry

Use

AAV

Tags

mCherry

Expression

Mammalian

Promoter

hSyn

Available since

Feb. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pEJS1830 All-in-one AAV-U1a-NmeABE-8e-2xBPSV40-U6-Fah

Plasmid

#199263

Purpose

Single AAV vector for expressing N-terminal fusion Nme2Cas9-ABE8e and one U6 driven sgRNA targeting the point mutation in the mouse Fah gene of a HT1 mouse model

Depositor

Erik Sontheimer

Publication

Zhang et al GEN Biotechnol. 2022 Jun 1;1(3):285-299.

Insert

NmeABE8e

Use

AAV and CRISPR

Tags

NLS

Expression

Mammalian

Promoter

U1a

Available since

April 10, 2023

Availability

Academic Institutions and Nonprofits only

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pSpCas9(BB)-2A-mCherry

Plasmid

#197420

Purpose

Cas9 from S. pyogenes with 2A-mCherry, and cloning backbone for sgRNA

Depositor

Torsten Wittmann

Publication

Dema et al Elife. 2023 Jan 30;12:e84143. doi: 10.75

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Cas9

Use

CRISPR

Tags

mCherry

Expression

Mammalian

Available since

June 26, 2023

Availability

Academic Institutions and Nonprofits only

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PX333-T2A-EGFP

Plasmid

#197417

Purpose

SpCas9-T2A-EGFP with dual sgRNA cloning backbones

Depositor

Yeh-Hsing Lao

Publication

Yoshinaga et al Nano Lett. 2023 Jan 17. doi: 10.1021/acs

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Promoter

CBh; U6

Available since

March 30, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pX458-Ef1a-dCas9-VPR-H2B-mCherry (CRISPRa)

Plasmid

#175572

Purpose

Inactivated Cas9 (dCas9) fusion to VPR for the purpose of targeted activation

Depositor

Joseph Brzezinski

Type

Empty backbone

Use

CRISPR

Available since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pORANGE GFP-Grin2b KI

Plasmid

#131487

Purpose

Endogenous tagging of GluN2b: N-terminal (amino acid position: S34)

Depositor

Harold MacGillavry

Publication

Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

gRNA and GFP donor (Grin2b Rat)

Expression

Mammalian

Promoter

U6 and Cbh

Available since

Sept. 19, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pORANGE GFP-Grin1 KI

Plasmid

#131485

Purpose

Endogenous tagging of GluN1: N-terminal (amino acid position: A20)

Depositor

Harold MacGillavry

Publication

Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

gRNA and GFP donor (Grin1, Grin1 Rat, Mouse)

Expression

Mammalian

Promoter

U6 and Cbh

Available since

Sept. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pX330_human CDK1

Plasmid

#118597

Purpose

One shot system to create CDK1as human cells. Encode Cas9 and guideRNA to inactivate human CDK1 gene.

Depositor

William Earnshaw

Publication

Saldivar et al Science. 2018 Aug 24;361(6404):806-810.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

guide RNA against human CDK1

Use

CRISPR

Available since

Jan. 9, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pX462-hPRKAA1-gRNA_A

Plasmid

#74374

Purpose

gRNA_A to knockout human AMPK alpha 1 using Cas9n

Depositor

Reuben Shaw

Publication

Toyama et al Science. 2016 Jan 15;351(6270):275-81. d

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

human AMPK alpha 1 exon1 gRNA (PRKAA1 Human)

Use

CRISPR

Promoter

U6

Available since

April 14, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pX330S-7

Plasmid

#58783

Purpose

Expresses Cas9 nuclease and gRNA

Depositor

Takashi Yamamoto

Publication

Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

humanized S. pyogenes Cas9 nuclease

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Promoter

CBh

Available since

Nov. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pBK2046-AAV-2xSp1-2xNFkB-EFSNC-dSaCas9-KRAB-MECP2(APOE-gRNA1)

Plasmid

#223166

Purpose

2nd gen. vector for expression of KRAB and truncated MECP2 with dSaCas9 and gRNA1 targeting human/mouse APOE

Depositor

Boris Kantor

Publication

Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

Insert

KRAB-MECP2 (MECP2 Human)

Use

AAV and CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

Encodes aa 204-310 (MECP2)

Promoter

EF1a

Available since

Aug. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-fos-TetOn3G-WPRE

Plasmid

#261817

Purpose

Activity dependent TetOn3G expression

Depositor

Tobias Bonhoeffer

Publication

Joag et al bioRxiv 2026.08.18.745461

Insert

TetOn3G

Use

AAV

Promoter

cfos

Available since

Oct. 28, 2026

Availability

Academic Institutions and Nonprofits only

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pAAV-HDR-sphys-3XHA

Plasmid

#163690

Purpose

plasmid for homologous recombination-mediated insertion of triple HA tag into synaptophysin gene

Depositor

Bernardo Sabatini

Publication

Chantranupong et al Elife. 2020 Oct 12;9. pii: 59699. doi: 1

Insert

triple HA tag insertion into endogenous mouse synaptophysin gene

Use

AAV

Tags

triple HA tag

Available since

Jan. 13, 2021

Availability

Academic Institutions and Nonprofits only

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AiP12256-pAAV-AiE0462m-minBG-SYFP2-WPRE3-BGHpA (Alias: CN2256)

Plasmid

#164456

Purpose

AiE0462m is an enhancer sequence designed to drive AAV-mediated transgene expression in L5_ET cortical neurons

Depositor

Allen Institute for Brain Science, Jonathan Ting

Publication

Graybuck et al Neuron. 2021 Mar 23. pii: S0896-6273(21)

Insert

SYFP2

Use

AAV

Promoter

minBetaGlobin

Available since

Feb. 25, 2021

Availability

Academic Institutions and Nonprofits only

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AiP12255-pAAV-AiE0460m-minBG-SYFP2-WPRE3-BGHpA (Alias: CN2255)

Plasmid

#164455

Purpose

AiE0460m is an enhancer sequence designed to drive AAV-mediated transgene expression in L5_ET cortical neurons

Depositor

Allen Institute for Brain Science, Jonathan Ting

Publication

Graybuck et al Neuron. 2021 Mar 23. pii: S0896-6273(21)

Insert

SYFP2

Use

AAV

Promoter

minBetaGlobin

Available since

Feb. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-GRAB_UCN1.0

Plasmid

#208669

Purpose

Expresses the genetically-encoded fluorescent urocortin (UCN) sensor GRAB_UCN1.0 in a cre-dependent manner

Depositor

Yulong Li

Publication

Wang et al Science. 2023 Nov 17;382(6672):eabq8173.

Insert

GPCR activation based urocortin (UCN) sensor GRAB_UCN1.0

Use

AAV

Promoter

hSyn

Available since

Oct. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-Camk2a(0.4)-DIO-Opto-cytTrkB(E281A)-HA

Plasmid

#180588

Purpose

Opto-cytTrkB

Depositor

Won Do Heo

Publication

Hong J et al J Mol Biol. 2020 Feb 14;432(4):815-827.

Insert

TrkB

Use

AAV

Mutation

PHR(E281A)

Available since

Feb. 28, 2022

Availability

Academic Institutions and Nonprofits only

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AiP13755: pAAV-AiE0743m_3xC2-minBG-ChR2(H134R)-EYFP-WPRE3-BGHpA (Alias: CN3755)

Plasmid

#214846

Purpose

Expression of ChR2(H134R)-EYFP in striatal cholinergic neurons

Depositor

Allen Institute for Brain Science, Jonathan Ting

Publication

Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1

Has service

AAV PHP.eB

Insert

ChR2(H134R)-EYFP

Use

AAV

Available since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-DD-T6B-MUT-YFP

Plasmid

#235144

Purpose

Expresses the inducible mutant T6B peptide fused with DHFR and YFP under the human synapsin promoter

Depositor

Giordano Lippi

Publication

Zolboot et al Neuron. 2025 Apr 1:S0896-6273(25)00181-3

Insert

DHFR-fused mutant T6B peptide

Use

AAV

Mutation

5 tryptophan residues of T6B-WT mutated

Promoter

hSyn

Available since

April 28, 2025

Availability

Academic Institutions and Nonprofits only

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