We narrowed to 5,886 results for: arl

1. mAIRN HO

   Plasmid

   #207411

   Purpose

   Mammalian-cell-based plasmid system that expresses mAIRN (an R-loop-forming transcript) upon dox-induction, which will further cause head-ON transcription-replication conflict (HO TRC).

   Depositor

   Karlene Cimprich

   Article

   Hamperl et al Cell. 2017 Aug 10;170(4):774-786.e19. do

   Insert

   mAIRN
   

   Expression

   Bacterial and Mammalian

   Available Since

   March 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. mAIRN CD

   Plasmid

   #207412

   Purpose

   Mammalian-cell-based plasmid system that expresses mAIRN (an R-loop-forming transcript) upon dox-induction, which will further cause co-directional transcription-replication conflict (CD TRC).

   Depositor

   Karlene Cimprich

   Article

   Hamperl et al Cell. 2017 Aug 10;170(4):774-786.e19. do

   Insert

   mAIRN
   

   Expression

   Bacterial and Mammalian

   Available Since

   March 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. ECFP HO

   Plasmid

   #207413

   Purpose

   Mammalian-cell-based plasmid system that expresses ECFP (a non R-loop-forming transcript) upon dox-induction, which will further cause head-ON transcription-replication conflict (HO TRC).

   Depositor

   Karlene Cimprich

   Article

   Hamperl et al Cell. 2017 Aug 10;170(4):774-786.e19. do

   Insert

   ECFP
   

   Expression

   Bacterial and Mammalian

   Available Since

   Jan. 31, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. ECFP CD

   Plasmid

   #207414

   Purpose

   Mammalian-cell-based plasmid system that expresses ECFP (a non R-loop-forming transcript) upon dox-induction, which will further cause co-directional transcription-replication conflict (CD TRC).

   Depositor

   Karlene Cimprich

   Article

   Hamperl et al Cell. 2017 Aug 10;170(4):774-786.e19. do

   Insert

   ECFP
   

   Expression

   Bacterial and Mammalian

   Available Since

   Jan. 31, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pComb3XTT

   Plasmid

   #63891

   Purpose

   Backbone of pComb3X containing TT Fab (light and heavy chains) against tetanus toxin, used as phage display/expression control and PCR template for human antibody constant regions (kappa and CH1)

   Depositor

   Carlos Barbas

   Article

   Andris-Widhopf et al J Immunol Methods. 2000 Aug 28;242(1-2):

   Insert

   TT Fab
   

   Use

   Phage display

   Tags

   6xHis, HA tag, and geneIII

   Expression

   Bacterial

   Promoter

   lacZ

   Available Since

   July 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCI-ASC-HA

   Plasmid

   #41553

   Depositor

   Kate Fitzgerald

   Article

   Hornung et al Nature. 2009 Mar 26. 458(7237):514-8.

   Insert

   ASC (PYCARD Human)
   

   Tags

   HA

   Expression

   Mammalian

   Promoter

   CMV immediate-early enhancer/promoter

   Available Since

   Dec. 20, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCAS9i

   Plasmid

   #141249

   Purpose

   Galactose-inducible expression of Cas9 for addressing one target; Contains guide RNA expression cassette with stuffer and KpnI-Pme1 restriction sites.

   Depositor

   Adam Bertl

   Article

   Degreif et al J Biol Methods. 2018 Sep 12;5(3):e98. do

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Yeast

   Promoter

   GAL1 (Cas9), pSNR52 (gRNA)

   Available Since

   Aug. 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCAS9c

   Plasmid

   #141250

   Purpose

   Constitutive expression of Cas9 for addressing one target; Contains guide RNA expression cassette with stuffer and KpnI-Pme1 restriction sites.

   Depositor

   Adam Bertl

   Article

   Degreif et al J Biol Methods. 2018 Sep 12;5(3):e98. do

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Yeast

   Promoter

   TEF1 (Cas9), pSNR52 (gRNA)

   Available Since

   Aug. 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCAS9id

   Plasmid

   #141251

   Purpose

   Galactose-inducible expression of Cas9 for addressing two targets; Contains two guide RNA expression cassette with stuffer and KpnI-Pme1 restriction sites.

   Depositor

   Adam Bertl

   Article

   Degreif et al J Biol Methods. 2018 Sep 12;5(3):e98. do

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Yeast

   Promoter

   GAL1 (Cas9), pSNR52 (gRNA)

   Available Since

   Aug. 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCAS9cd

    Plasmid

    #141252

    Purpose

    Constitutive expression of Cas9 for addressing two targets; Contains two guide RNA expression cassette with stuffer and KpnI-Pme1 restriction sites.

    Depositor

    Adam Bertl

    Article

    Degreif et al J Biol Methods. 2018 Sep 12;5(3):e98. do

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Yeast

    Promoter

    TEF1 (Cas9), pSNR52 (gRNA)

    Available Since

    Aug. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCDNA3.1 (-) + codon optimized Brn3a

    Plasmid

    #86829

    Purpose

    mammalian expression of the transcription factor Brn3a

    Depositor

    Carl Novina

    Article

    Schmidt et al Cell Rep. 2016 May 31;15(9):2025-37. doi

    Insert

    Brn3a (POU4F1 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. LV-TRE-VP64 human MyoD-T2A-dsRedExpress2

    Plasmid

    #60629

    Purpose

    Expresses VP64 human MyoD and dsRedExpress2 in response to doxycycline

    Depositor

    Charles Gersbach

    Article

    Kabadi et al ACS Synth Biol. 2014 Dec 10.

    Insert

    VP64 human MyoD (MYOD1 Human)
    

    Use

    Lentiviral

    Promoter

    TRE

    Available Since

    Dec. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. lenti-NLS-mTagBFP2

    Plasmid

    #216128

    Purpose

    Lentiviral vector for expressing mTagBFP2 localizing to the nucleus

    Depositor

    Stefan Kubicek

    Article

    Reicher et al Nat Cell Biol. 2024 May;26(5):745-756. d

    Insert

    EF1a-NuclearLocalizationSignal-mTagBFP2-WPRE
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    April 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-EF1a-4mtGCaMP6f-DIO

    Plasmid

    #179529

    Purpose

    Cre-dependent expression of mitochondria-targeted GCaMP6f under control of the EF1a promoter

    Depositor

    Maarten Kole

    Article

    Kole et al Nat Commun. 2022 Dec 9;13(1):7598. doi:

    Insert

    4mtGCaMP6f
    

    Use

    AAV

    Tags

    4x Cox8 targeting sequence

    Promoter

    Ef1a

    Available Since

    Oct. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. Salsa6f

    Plasmid

    #140188

    Purpose

    Salsa6f is a fluorescent ratiometric genetically encoded calcium indicator, (tdTomato linked to GCaMP6f by a V5 epitope tag)

    Depositor

    Michael Cahalan

    Article

    Dong et al eLife. 2017 Dec 14;6. pii: 32417. doi: 1

    Insert

    Salsa6f
    

    Tags

    tdTomato-v5-GCaMP6f

    Expression

    Mammalian

    Promoter

    CMV Immediate Early

    Available Since

    Nov. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. lenti-NLS-miRFP670nano-miRFP670

    Plasmid

    #216131

    Purpose

    Lentiviral vector for expressing a fusion of miRFP670nano and miRFP670 localizing to the nucleus.

    Depositor

    Stefan Kubicek

    Article

    Reicher et al Nat Cell Biol. 2024 May;26(5):745-756. d

    Insert

    EF1a-NuclearLocalizationSignal-miRFP670nano-miRFP670-WPRE
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    April 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. HIV-dual-GT

    Plasmid

    #122696

    Purpose

    Dual-fluorescence reporter to monitor the expression of HIV-1 early genes and late genes simultaneously.

    Depositor

    Klaus Überla

    Article

    Xiao et al mBio. 2021 Jan 19;12(1). pii: mBio.02525

    Inserts

    mTagBFP2
    DsRed-max
    

    Use

    Lentiviral

    Tags

    PEST domain

    Promoter

    HIV-1 LTR

    Available Since

    Jan. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLenti-EF1a-hChR2(H134R)-EYFP-WPRE

    Plasmid

    #20942

    Purpose

    3rd gen lentiviral expression of humanized ChR2 with H134R mutation fused to EYFP driven by EF1a promoter for optogenetic activation

    Depositor

    Karl Deisseroth

    Article

    Zhang et al Nature. 2007 Apr 5. 446(7136):633-9.

    Insert

    channelrhodopsin-2
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    H134R mutation in ChR2

    Promoter

    EF1alpha

    Available Since

    May 13, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET14b – E. coli Exonuclease I

    Plasmid

    #104552

    Purpose

    bacterial expression of Exonuclease I

    Depositor

    Charles Bell

    Article

    Korada et al Nucleic Acids Res. 2013 Jun;41(11):5887-

    Insert

    Exonuclease I (sbcB E.coli)
    

    Tags

    6X-His tag followed by thrombin site

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Dec. 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLenti-Synapsin-hChR2(H134R)-EYFP-WPRE

    Plasmid

    #20945

    Purpose

    3rd gen lentiviral expression of humanized ChR2 with H134R mutation fused to EYFP driven by human Synapsin I promoter for optogenetic activation

    Depositor

    Karl Deisseroth

    Article

    Zhang et al Nature. 2007 Apr 5. 446(7136):633-9.

    Insert

    channelrhodopsin-2
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    H134R mutation in ChR2

    Promoter

    hSyn

    Available Since

    May 28, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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