We narrowed to 143,240 results for: ins

1. Lambda Phosphatase

   Plasmid

   #79748

   Purpose

   Intended for co-expression with human Ser/Thr kinase plasmids to enhance bacterial kinase expression.

   Depositor

   John Chodera, Markus Seeliger, Nicholas Levinson

   Article

   Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

   Insert

   Lambda Phosphatase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Nov. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMIIS793

   Plasmid

   #238206

   Purpose

   Expresses TaTasR with an N-terminal Twin-Strep-SUMO tag. KanR

   Depositor

   Feng Zhang

   Article

   Faure et al Science. 2025 Feb 27:eadv9789. doi: 10.1

   Insert

   TaTasR
   

   Use

   Tags

   TwinStrepII-bdSUMO

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. BPK1520

   Plasmid

   #65777

   Purpose

   Human expression plasmid for SpCas9 sgRNA (need to clone in spacer into BsmBI sites): U6-BsmBIcassette-Sp-sgRNA

   Depositor

   Keith Joung

   Article

   Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

   Insert

   SpCas9 gRNA backbone, without spacer sequence
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   June 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. hGip-R

   Plasmid

   #14942

   Depositor

   Bernard Thorens

   Article

   Gremlich et al Diabetes. 1995 Oct . 44(10):1202-8.

   Insert

   Gip-R (GIPR Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Short form of the receptor

   Promoter

   Available since

   June 7, 2007

   Availability

   Academic Institutions and Nonprofits only

5. f-CTS-EGFP

   Plasmid

   #86064

   Purpose

   A mammalian expression plasmid encoding fibrocystin ciliary targeting signal domain with C-terminus EGFP.

   Depositor

   Lei Lu

   Article

   Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

   Insert

   Fibrocystin (Pkhd1 Mouse)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   contains ciliary targeting signal (3870-3887 amin…

   Promoter

   CMV

   Available since

   May 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pACE-GIRK4-mC-S

   Plasmid

   #172427

   Purpose

   Expression of full-length, human GIRK4 with C-terminal mCherry StrepTag II

   Depositor

   Arthur Laganowsky

   Article

   Qiao et al Biochemistry. 2021 Dec 21;60(50):3813-38

   Insert

   GIRK4 (KCNJ5 Human)
   

   Use

   Tags

   mCherry Streptag II

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Oct. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pIRE4-Azi

   Plasmid

   #105829

   Purpose

   Plasmid encoding for the translational pair that enables efficent incoporation of p-Azido-phenylalanine in response to the amber codon into proteins in mammmalian cells

   Depositor

   Irene Coin

   Article

   Seidel et al Elife. 2017 Aug 3;6. doi: 10.7554/eLife.

   Inserts

   Humanized EAziRS
   4 copies of the U6-BstYam tRNA cassette
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   (Y37L, D182S, F183M, L186A, D265R)TyrRS

   Promoter

   PGK and U6

   Available since

   March 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pR I-Ab alpha BirA

   Plasmid

   #113638

   Purpose

   Insect cell expression vector for I-Ab alpha chain fused to BirA biotinylation site

   Depositor

   James Moon

   Article

   Moon et al Methods Mol Biol. 2018;1799:165-181. doi

   Insert

   I-Ab alpha BirA (H2-Ab1 Mouse)
   

   Use

   Tags

   BirA biotinylation signal site and fused to Fos-J…

   Expression

   Insect

   Mutation

   transmembrane and cytoplasmic domains truncated

   Promoter

   Metallothionein

   Available since

   Sept. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. BPK764

   Plasmid

   #65767

   Purpose

   Bacterial expression plasmid for SpCas9 & sgRNA (need to clone spacer into BsaI sites): T7-humanSpCas9-NLS-3xFLAG-T7-BsaIcassette-Sp-sgRNA

   Depositor

   Keith Joung

   Article

   Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

   Insert

   mammalian codon-optimized Streptococcus pyogenes Cas9-NLS-3XFlag, and SpCas9 gRNA
   

   Use

   CRISPR

   Tags

   3x FLAG and NLS

   Expression

   Bacterial

   Mutation

   Promoter

   T7 (x2)

   Available since

   June 24, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. Hy_pMT Laccase2 MCS Exon Vector

    Plasmid

    #69884

    Purpose

    Expression plasmid for expressing circular RNAs of a desired sequence in Drosophila

    Depositor

    Jeremy Wilusz

    Article

    Kramer et al Genes Dev. 2015 Oct 8.

    Insert

    Laccase2 (stw Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Metallothionein Promoter (pMT)

    Available since

    Oct. 19, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLVX-RT004-LaG30VB

    Plasmid

    #163384

    Purpose

    2nd generation lentiviral vector to express anti-GFP(LaG30) module in the G-baToN system

    Depositor

    Monte Winslow

    Article

    Tang et al Elife. 2020 Oct 7;9. pii: 61080. doi: 10

    Insert

    LaG30-VEEGFRtm-BFP (LaG30VB)
    

    Use

    Lentiviral

    Tags

    BFP

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Jan. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. CapChR2_pmScarlet-N1

    Plasmid

    #188032

    Purpose

    Expresses CapChR2-mScarlet in mammalian cells

    Depositor

    Peter Hegemann

    Article

    Fernandez Lahore et al Nat Commun. 2022 Dec 21;13(1):7844. doi:

    Insert

    CapChR2-mScarlet
    

    Use

    Tags

    mScarlet

    Expression

    Mammalian

    Mutation

    S43D, L112C, T139C and N238E mutations in CoChR

    Promoter

    CMV

    Available since

    Dec. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSEM318 - sgRNA mosTI unc-119 single-copy

    Plasmid

    #159822

    Purpose

    Single copy or array insertion by MosTI using split unc-119 selection

    Depositor

    Christian Froekjaer-Jensen

    Article

    El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

    Insert

    sgRNA mosTI unc-119 single-copy
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Not applicable

    Promoter

    Available since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-CAG-HcKCR1-EYFP

    Plasmid

    #182021

    Purpose

    Express light-gated potassium channel HcKCR1-EYFP in mammalian cells

    Depositor

    Mingshan Xue

    Article

    Govorunova et al Nat Neurosci. 2022 Jun 20. pii: 10.1038/

    Insert

    HcKCR1
    

    Use

    AAV

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3-P4:gs:nls:CIBN

    Plasmid

    #135986

    Purpose

    Expresses the P4 coiled coil fused to the CIBN blue light-inducible domain in mammalian cells

    Depositor

    Roman Jerala

    Article

    Lebar et al Nat Chem Biol. 2020 Jan 6. pii: 10.1038/

    Insert

    P4 coiled-coil fused to the CIBN domain
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLX302 MX1-V5 puro

    Plasmid

    #158640

    Purpose

    Lentiviral expression vector for constitutive expression of V5-tagged MX1.

    Depositor

    Kevin Janes

    Article

    Lopacinski et al Cell Syst. 2021 Apr 21;12(4):304-323.e13

    Insert

    Mx1 (MX1 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mutation

    Promoter

    Available since

    Oct. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. dCas9-10xSunTag-P2A-tagBFP

    Plasmid

    #174141

    Purpose

    Expresses the dCas9-10xSunTag-tagBFP fusion protein for the recruitment of scFv(GCN4)-fused effector domains. A shorter version of the Addgene 60903 plasmid.

    Depositor

    Albert Jeltsch

    Article

    Hofacker et al Int J Mol Sci. 2020 Jan 13;21(2). pii: i

    Insert

    dCas9, 10xSunTag, tagBFP
    

    Use

    Tags

    HA and tagBFP

    Expression

    Mammalian

    Mutation

    Promoter

    SV40

    Available since

    Oct. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLL7.0: tgRFPt-SSPB WT

    Plasmid

    #60415

    Purpose

    Mammalian Expression of tgRFPt-Nano

    Depositor

    Brian Kuhlman

    Article

    Guntas et al Proc Natl Acad Sci U S A. 2015 Jan 6;112

    Insert

    tgRFPt-SSPB
    

    Use

    Cre/Lox and Lentiviral

    Tags

    TagRFPt

    Expression

    Mammalian

    Mutation

    Y11K and A15E

    Promoter

    CMV

    Available since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pFastBac HT JS-Munc18c

    Plasmid

    #135555

    Purpose

    Express mouse Munc18c in Sf9 cells, the resulted plasmid encodes an N-terminally His6-tagged Munc18c protein with a tobacco etch virus (TEV) cleavage site between the His6 tag and Munc18c.

    Depositor

    Jingshi Shen

    Article

    Yu et al Methods Mol Biol. 2019;1860:237-249. doi

    Insert

    Munc18c
    

    Use

    Tags

    6xHis tag and TEV site

    Expression

    Insect

    Mutation

    Promoter

    polyhedrin promoter

    Available since

    Feb. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. p10xQUAS-CsChrimson

    Plasmid

    #163629

    Purpose

    10xQUAS effector expressing CsChrimson.mVenus for red-shifted optogenetic activation of neurons; Contains both P-element ends and attB integration sequence

    Depositor

    Christopher Potter

    Article

    Task et al Elife. 2022 Apr 20;11. pii: 72599. doi:

    Insert

    CsChrimson.mVenus
    

    Use

    Tags

    mVenus

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Feb. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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