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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 6,945 results for: pcDNA3.1+

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Showing: 5081 - 5100 of 6945 results

  6. CoV2-N-D3L

    Plasmid
    #177945
    Purpose
    Transient mammalian expression of codon optimized SARS-CoV-2 Nucleocapsid (D3L). Used for generating RNA packaging virus-like particles.
    Depositor
    Jennifer Doudna
    Publication
    Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11
    Insert
    SARS-CoV-2 Nucleocapsid (D3L) (N )
    Expression
    Mammalian
    Mutation
    D3L
    Available since
    Nov. 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    177945-plasmid-map-sequence-id-348204

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  7. CoV2-N-A119S

    Plasmid
    #177948
    Purpose
    Transient mammalian expression of codon optimized SARS-CoV-2 Nucleocapsid (A119S). Used for generating RNA packaging virus-like particles.
    Depositor
    Jennifer Doudna
    Publication
    Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11
    Insert
    SARS-CoV-2 Nucleocapsid (A119S) (N )
    Expression
    Mammalian
    Mutation
    A119S
    Available since
    Nov. 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    177948-plasmid-map-sequence-id-348195

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  8. CoV2-N-D377Y

    Plasmid
    #177957
    Purpose
    Transient mammalian expression of codon optimized SARS-CoV-2 Nucleocapsid (D377Y). Used for generating RNA packaging virus-like particles.
    Depositor
    Jennifer Doudna
    Publication
    Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11
    Insert
    SARS-CoV-2 Nucleocapsid (D377Y) (N )
    Expression
    Mammalian
    Mutation
    D377Y
    Available since
    Nov. 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    177957-plasmid-map-sequence-id-348184

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  9. pRD406

    Plasmid
    #163111
    Purpose
    Expression of mNeonGreen_H-NSdbd in eukaryotes for visualisation of nuclear DNA
    Depositor
    Remus Dame
    Publication
    Rashid et al Nucleic Acids Res. 2021 Nov 4. pii: 6414
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    NLS_mNeonGreen_H-NSdbd_NLS
    Use
    Eukaryotic expression
    Expression
    Mammalian
    Promoter
    CMV promoter; T7 promoter
    Available since
    Oct. 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    163111-plasmid-map-sequence-id-321832

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  10. pRD398

    Plasmid
    #163105
    Purpose
    Expression of mCherry_H-NSdbd in eukaryotes for visualisation of nuclear DNA
    Depositor
    Remus Dame
    Publication
    Rashid et al Nucleic Acids Res. 2021 Nov 4. pii: 6414
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    NLS_mCherry_H-NSdbd_NLS
    Use
    Eukaryotic expression
    Expression
    Mammalian
    Promoter
    CMV promoter; T7 promoter
    Available since
    Oct. 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    163105-plasmid-map-sequence-id-321846

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  11. pRD399

    Plasmid
    #163106
    Purpose
    Expression of mEGFP_H-NSdbd in eukaryotes for visualisation of nuclear DNA
    Depositor
    Remus Dame
    Publication
    Rashid et al Nucleic Acids Res. 2021 Nov 4. pii: 6414
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    NLS_mEGFP_H-NSdbd_NLS
    Use
    Eukaryotic expression
    Expression
    Mammalian
    Promoter
    CMV promoter; T7 promoter
    Available since
    Oct. 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    163106-plasmid-map-sequence-id-321847

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  12. CCR5-SZ190b-sfGFP

    Plasmid
    #162447
    Purpose
    Expression in HEK293T cell and compete ligand singaling against full-length receptors, the truncated receptor can perform signaling at high ligand concentration
    Depositor
    Shuguang Zhang
    Publication
    Qing et al iScience. 2020 Oct 28;23(12):101670. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    C-C chemokine receptor type 5 (CCR5 Human)
    Expression
    Mammalian
    Mutation
    truncation from aa88 to aa249
    Promoter
    CMV
    Available since
    Aug. 20, 2021
    Availability
    Academic Institutions and Nonprofits only
    162447-plasmid-map-sequence-id-331353

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  13. pcDNA3_N-DYK_MTHFD1-NLS

    Plasmid
    #133023
    Purpose
    NLS sequence was fused to MTHFD1-wild type through site-directed mutagenesis (Q5 Site-Directed Mutagenesis Kit, New England Biolabs)
    Depositor
    Stefan Kubicek
    Publication
    Sdelci et al Nat Genet. 2019 Jun;51(6):990-998. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    MTHFD1 (MTHFD1 Human)
    Tags
    NLS
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Aug. 2, 2021
    Availability
    Academic Institutions and Nonprofits only
    133023-plasmid-map-sequence-id-338753

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  17. PRMT8(E185Q)-FLAG

    Plasmid
    #164700
    Purpose
    Expression of PRMT8(E185Q) with a C-terminal FLAG tag
    Depositor
    Cheryl Arrowsmith
    Insert
    PRMT8 (PRMT8 Human)
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    E185Q
    Promoter
    CMV
    Available since
    March 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    164700-plasmid-map-sequence-id-326681

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  20. BE3-NLS-NLS-P2A-BLAST

    Plasmid
    #135348
    Purpose
    Expression of BE3 (Base editor generation three) with dual C-terminal NLS in a single operon encoding P2A blasticidine resistance
    Depositor
    Alex Hewitt
    Publication
    Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    BE3-2XNLS
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    WT
    Available since
    Oct. 1, 2020
    Availability
    Academic Institutions and Nonprofits only
    135348-plasmid-map-sequence-id-267697

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Showing: 5081 - 5100 of 6945 results