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We narrowed to 13,996 results for: eng

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Showing: 5101 - 5120 of 13996 results

  1. pMG071 MBP-GSK3β_S9A-HA-His, GST_λPPase

    Plasmid
    #196185
    Purpose
    Co-expresses MBP-GSK3β_S9A-HA-His (human GSK3β with S9A mutation as a fusion protein with MBP, HA, and His-tags) and GST_λPPase in E.coli to produce unphosphorylated MBP-GSK3β_S9A-HA-His
    Depositor
    Jesse Zalatan
    Article
    Gavagan et al Elife. 2023 Aug 7;12:e85444. doi: 10.755
    Inserts
    GSK3B (GSK3B Human)
    λPPase
    Tags
    GST, HA, His6, and MBP
    Expression
    Bacterial
    Mutation
    changed Serine 9 to Alanine
    Promoter
    Tac
    Available Since
    Jan. 31, 2023
    Availability
    Academic Institutions and Nonprofits only
    196185-plasmid-map-sequence-id-383261

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  2. pAAV-hSyn-DIO-Gluc-RMA-IRES-EGFP

    Plasmid
    #189630
    Purpose
    Expresses Gluc-RMA and EGFP in the presence of Cre recombinase. Double-floxed Gluc-RMA and EGFP for monitoring Cre-expressing neuronal populations.
    Depositor
    Jerzy Szablowski
    Article
    Lee et al Nat Biotechnol. 2024 Jan 10. doi: 10.103
    Inserts
    Gaussia luciferase fused to Fc
    IRES-EGFP
    Use
    AAV, Cre/Lox, and Luciferase
    Tags
    Gluc and IgG1 Fc
    Expression
    Mammalian
    Promoter
    IRES and hSyn
    Available Since
    Nov. 3, 2022
    Availability
    Academic Institutions and Nonprofits only
    189630-plasmid-map-sequence-id-376528

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  3. pcDNA3-HCMVgO-sfGFP (TB40/E)

    Plasmid
    #136451
    Purpose
    Mammalian expression plasmid for HCMV glycoprotein O (TB40/E strain) fused to superfolder GFP
    Depositor
    Erik Procko
    Article
    Park et al PLoS Pathog. 2020 Jun 19;16(6):e1008647.
    Insert
    glycoprotein O (UL74 Human betaherpesvirus 5)
    Tags
    Gly/Ser-rich linker (GGSGGSGSGG) (includes BamHI …
    Expression
    Mammalian
    Mutation
    Codon-optimized for human cell expression.
    Promoter
    CMV
    Available Since
    July 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    136451-plasmid-map-sequence-id-271690

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  4. pcDNA3-sACE2v2-8his

    Plasmid
    #149270
    Purpose
    Mammalian expression plasmid for his-tagged sACE2 high affinity variant 2
    Depositor
    Erik Procko
    Article
    Chan et al Science. 2020 Sep 4;369(6508):1261-1265.
    Insert
    Angiotensin-converting enzyme 2 (ACE2 Human)
    Tags
    Short GSG linker and 8his tag
    Expression
    Mammalian
    Mutation
    Extracellular, soluble protease domain (a.a. M1-D…
    Promoter
    CMV
    Available Since
    May 28, 2020
    Availability
    Academic Institutions and Nonprofits only
    149270-plasmid-map-sequence-id-298417

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  5. CiDr-VSP L223F/C302S-mCherry pcDNA3.1 (eVSP CSmutant)

    Plasmid
    #140893
    Purpose
    Expresses modified Danio rerio VSP without phosphatase activity (inactive mutant of enhanced VSP) fused with mCherry in mammalian cel
    Depositor
    Yasushi Okamura
    Article
    Kawanabe et al J Gen Physiol. 2020 May 4;152(5). pii: 1
    Insert
    CiDr-VSP L223F/C302S-mCherry (vsp, tpte Ciona intestinalis, Zebrafish)
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    changed 223L to F in Dr-VSP (zebrafish TPTE), cha…
    Available Since
    May 13, 2020
    Availability
    Academic Institutions and Nonprofits only
    140893-plasmid-map-sequence-id-297104

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  6. pcDNA3-sACE2v3-sfGFP

    Plasmid
    #145174
    Purpose
    Mammalian expression plasmid for sACE2-sfGFP high affinity variant 3
    Depositor
    Erik Procko
    Article
    Chan et al Science. 2020 Sep 4;369(6508):1261-1265.
    Insert
    Angiotensin-converting enzyme 2 (ACE2 Human)
    Tags
    Linker GSGGSGSGG and superfolder GFP
    Expression
    Mammalian
    Mutation
    Extracellular, soluble protease domain (a.a. M1-D…
    Promoter
    CMV
    Available Since
    April 15, 2020
    Availability
    Academic Institutions and Nonprofits only
    145174-plasmid-map-sequence-id-292600

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  11. pCMV-T7-SpCas9(LWKYQS)-P2A-EGFP (AHK34)

    Plasmid
    #223079
    Purpose
    pCMV and pT7 Human expression plasmid for SpCas9 enzyme with LWKYQS amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586
    Insert
    human codon optimized nuclease SpCas9(LWKYQS)-P2A-EGFP
    Use
    CRISPR
    Tags
    BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Mutation
    SpCas9(LWKYQS)=D1135L, S1136W, G1218K, E1219Y, R1…
    Promoter
    CMV and T7
    Available Since
    April 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    223079-plasmid-map-sequence-id-447658

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  12. peSCBE3-NG-Hypa

    Plasmid
    #218165
    Purpose
    This plasmid harbors the base editor eSCBE3-NG-Hypa along with an sgRNA cloning cassette, facilitating high-efficiency and high-fidelity cytosine base editing at targets bearing NG PAM in Streptomyces
    Depositor
    Yuhui Sun
    Article
    Wang et al Nat Commun. 2024 Jul 7;15(1):5687. doi:
    Inserts
    escbe3-NG-Hypa
    a programmable sgRNA cloning cassette
    Use
    CRISPR; Sgrna transcription
    Expression
    Bacterial
    Mutation
    Mutation in the portion of deaminase hAPOBEC3A : …
    Promoter
    rpsL promoter
    Available Since
    May 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    218165-plasmid-map-sequence-id-433201

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  14. pCOCK CM-R05

    Plasmid
    #46568
    Purpose
    Mammalian expression plasmid with EGFP expressed from synthetic promoter (random mutagenesis of CMVwt 3' end).
    Depositor
    Clifford Wang
    Article
    Ferreira et al Syst Synth Biol. 2011 Dec;5(3-4):131-8.
    Insert
    CMV mutant promoter
    Use
    Synthetic Biology
    Expression
    Mammalian
    Mutation
    Mutant version of CMV promoter (refer to Table 1 …
    Available Since
    Sept. 23, 2013
    Availability
    Academic Institutions and Nonprofits only

  18. pDIRECT_21D

    Plasmid
    #91131
    Purpose
    Direct Cloning, Type: T-DNA, Engineering Reagent: 35S:Csy4-P2A-AtCas9_dead + CmYLCV:gRNAs with Csy4 spacers, Plant Selection: 2x35S:hpt II
    Depositor
    Daniel Voytas
    Article
    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.
    Insert
    Engineering Reagent: 35S:Csy4-P2A-AtCas9_dead + CmYLCV:gRNAs with Csy4 spacers
    Use
    CRISPR
    Expression
    Plant
    Mutation
    D10A, H840A
    Available Since
    July 7, 2017
    Availability
    Academic Institutions and Nonprofits only
    91131-plasmid-map-sequence-id-182255

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  19. AAV-AIO-M11-gRNA-EFS-NMS-SadCas9

    Plasmid
    #210710
    Purpose
    This Plasmid express M11 promoter driven SadCas9 specific gRNA and EFS promoter driven NMS transactivation module fused to SadCas9
    Depositor
    Isaac Hilton
    Article
    Mahata et al Nat Methods. 2023 Oct 9. doi: 10.1038/s4
    Insert
    SadCas9 specific gRNA and NMS fused to N-terminus of SadCas9 (STAT1, MRTFA Staphylococcus aureus, Human, Synthetic)
    Use
    AAV and CRISPR
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    D10A/N580A
    Promoter
    M11
    Available Since
    Nov. 29, 2023
    Availability
    Academic Institutions and Nonprofits only
    210710-plasmid-map-sequence-id-416264

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  20. pCAG-rAPOBEC1-gs-XTEN-gs-hdLbCas12a(D832A)-NLS(nucleoplasmin)-gs-UGI-NLS(SV40)(LbBE1.1) (RTW1295)

    Plasmid
    #114085
    Purpose
    CAG promoter expression plasmid for human codon optimized LbBE1.1
    Depositor
    Keith Joung, Benjamin Kleinstiver
    Article
    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103
    Insert
    human codon optimized DNase-inactive (D832A) LbCas12a fused to N-terminal rAPOBEC1 and C-terminal UGI (LbBE1.1)
    Tags
    SV40 NLS
    Expression
    Mammalian
    Mutation
    D832A
    Available Since
    Dec. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    114085-plasmid-map-sequence-id-230653

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Showing: 5101 - 5120 of 13996 results