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We narrowed to 18,759 results for: nls

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Showing: 10241 - 10280 of 18759 results

  1. N-term AAV-ABE8e-SpCas9(S55R) (CA21)

    Plasmid
    #242659
    Purpose
    CBh promoter expression plasmid for N-terminal intein-split AAV construct with N-term of ABE8e-SpCas9(S55R) - to be used with eVRQR constructs
    Depositor
    Benjamin Kleinstiver
    Publication
    Alves et al Nat Biomed Eng. 2025 Sep 11:10.1038/s415
    Insert
    pAAV-pCBh-BPNLS(SV40)-TadA8e-gs-SpCas9(D10A;S55R)-[N-term]-NpuN-BPNLS(SV40)-WPRE-bGH_PA
    Use
    AAV and CRISPR
    Tags
    BPNLS and NpuN(intein)-BPNLS
    Mutation
    SpCas9(S55R)
    Promoter
    CBh
    Available since
    Oct. 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    242659-plasmid-map-sequence-id-489035

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  2. pvPE-V3

    Plasmid
    #240287
    Purpose
    Encodes third generation porcine endogenous retrovirus-derived prime editing (pvPE) system into mammalian cells for gene editing.
    Depositor
    Lusheng Huang
    Publication
    Liu et al Trends Biotechnol. 2025 Aug 30:S0167-779
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    pvPE-V3
    Use
    CRISPR
    Tags
    SV40 NLS
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Oct. 8, 2025
    Availability
    Academic Institutions and Nonprofits only
    240287-plasmid-map-sequence-id-488808

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  11. PB_p300_core-dCas9-EGFP_hygro

    Plasmid
    #226431
    Purpose
    PiggyBac transposon vector containing a dox inducible p300 core-dCas9 fusion protein and EGFP reporter. Hygromycin selection marker
    Depositor
    Alon Goren
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    p300 core-dCas9-EGFP fusion protein
    rtTA3-IRES-Hygro
    Use
    CRISPR and Synthetic Biology; Piggybac transposon
    Tags
    HA tag, P2A-EGFP, and p300 core
    Expression
    Mammalian
    Promoter
    Dox inducible minimal CMV and UbC Promoter
    Available since
    Sept. 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    226431-plasmid-map-sequence-id-458102

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  15. MCP-Rep-X-RT∆R

    Plasmid
    #239384
    Purpose
    For circular RNA-mediated inverse prime editor using MCP-Rep-X-M MLV-RT∆RNase H in HEK294T cells
    Depositor
    Caixia Gao
    Publication
    Liang et al Nat Commun. 2025 May 31;16(1):5057. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    MCP, Rep-X, M-MLV-RT(∆RNase H)
    Use
    CRISPR
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    ∆RNase H in M-MLV RT
    Promoter
    CMV
    Available since
    Aug. 4, 2025
    Availability
    Academic Institutions and Nonprofits only
    239384-plasmid-map-sequence-id-481789

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  18. Tad-CBE(TCG2)

    Plasmid
    #235068
    Purpose
    Expresses Tad-CBE(TCG2) in mammalian cells.
    Depositor
    Weixin Tang
    Publication
    Wu et al Nat Biotechnol. 2025 Jul 7:10.1038/s4158
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Tad-CBE(TCG2)-SpCas9 D10A
    Use
    CRISPR
    Tags
    2xUGI, bpNLS and bpNLS
    Expression
    Mammalian
    Available since
    July 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    235068-plasmid-map-sequence-id-467755

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  19. Tad-CBE(CCT2)

    Plasmid
    #235071
    Purpose
    Expresses Tad-CBE(CCT2) in mammalian cells.
    Depositor
    Weixin Tang
    Publication
    Wu et al Nat Biotechnol. 2025 Jul 7:10.1038/s4158
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Tad-CBE(CCT2)-SpCas9 D10A
    Use
    CRISPR
    Tags
    2xUGI, bpNLS and bpNLS
    Expression
    Mammalian
    Available since
    July 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    235071-plasmid-map-sequence-id-466783

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  20. SpRY-Tad-CBE(ACC2)

    Plasmid
    #235081
    Purpose
    Expresses SpRY-Tad-CBE(ACC2) in mammalian cells.
    Depositor
    Weixin Tang
    Publication
    Wu et al Nat Biotechnol. 2025 Jul 7:10.1038/s4158
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Tad-CBE(ACC2)-SpRY Cas9 D10A
    Use
    CRISPR
    Tags
    2xUGI, bpNLS and bpNLS
    Expression
    Mammalian
    Available since
    July 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    235081-plasmid-map-sequence-id-466818

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  21. Tad-CBE(ACT4)

    Plasmid
    #235060
    Purpose
    Expresses Tad-CBE(ACT4) in mammalian cells.
    Depositor
    Weixin Tang
    Publication
    Wu et al Nat Biotechnol. 2025 Jul 7:10.1038/s4158
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Tad-CBE(ACT4)-SpCas9 D10A
    Use
    CRISPR
    Tags
    2xUGI, SV40NLS and bpNLS
    Expression
    Mammalian
    Available since
    July 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    235060-plasmid-map-sequence-id-466786

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  22. Tad-CBE(ACC2)

    Plasmid
    #235061
    Purpose
    Expresses Tad-CBE(ACC2) in mammalian cells.
    Depositor
    Weixin Tang
    Publication
    Wu et al Nat Biotechnol. 2025 Jul 7:10.1038/s4158
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Tad-CBE(ACC2)-SpCas9 D10A
    Use
    CRISPR
    Tags
    2xUGI, SV40NLS and bpNLS
    Expression
    Mammalian
    Available since
    July 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    235061-plasmid-map-sequence-id-466785

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  23. Tad-CBE(TCT1)

    Plasmid
    #235065
    Purpose
    Expresses Tad-CBE(TCT1) in mammalian cells.
    Depositor
    Weixin Tang
    Publication
    Wu et al Nat Biotechnol. 2025 Jul 7:10.1038/s4158
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Tad-CBE(TCT1)-SpCas9 D10A
    Use
    CRISPR
    Tags
    2xUGI, bpNLS and bpNLS
    Expression
    Mammalian
    Available since
    July 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    235065-plasmid-map-sequence-id-466798

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  24. Tad-CBE(TCC2)

    Plasmid
    #235067
    Purpose
    Expresses Tad-CBE(TCC2) in mammalian cells.
    Depositor
    Weixin Tang
    Publication
    Wu et al Nat Biotechnol. 2025 Jul 7:10.1038/s4158
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Tad-CBE(TCC2)-SpCas9 D10A
    Use
    CRISPR
    Tags
    2xUGI, bpNLS and bpNLS
    Expression
    Mammalian
    Available since
    July 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    235067-plasmid-map-sequence-id-466795

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  33. PB-TRE3G-CCRB

    Plasmid
    #236772
    Purpose
    A piggybac-based cloning vector containing TRE3G promoter followed by cloning site and CAG promoter-driven nuclear-localized Clover-P2A-rtTA-IRES-BSD.
    Depositor
    Kazutoshi Takahashi
    Publication
    Okubo et al Sci Adv. 2025 Apr 11;11(15):eadq5484. do
    Type
    Empty backbone
    Use
    Piggybac
    Expression
    Mammalian
    Promoter
    TRE3G promoter, CAG promoter
    Available since
    May 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    236772-plasmid-map-sequence-id-473454

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  35. IK349

    Plasmid
    #233655
    Purpose
    Dox-inducible red light-inducible Flp-C; Flp-397C-PhyA
    Depositor
    Wilson Wong
    Publication
    Tous et al Sci Adv. 2025 May 9;11(19):eadt1971. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Flp-397C-PhyA
    Expression
    Mammalian
    Mutation
    None
    Promoter
    TRE3G
    Available since
    May 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    233655-plasmid-map-sequence-id-464615

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  36. IK350

    Plasmid
    #233656
    Purpose
    Dox-inducible red light-inducible Flp-N; Flp-N396-FHY1
    Depositor
    Wilson Wong
    Publication
    Tous et al Sci Adv. 2025 May 9;11(19):eadt1971. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Flp-N396-FHY1
    Expression
    Mammalian
    Mutation
    None
    Available since
    May 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    233656-plasmid-map-sequence-id-464618

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  37. CT769

    Plasmid
    #233661
    Purpose
    Split Flp on same plasmid; PhyA-Flp-397-Flp-396-FHY1
    Depositor
    Wilson Wong
    Publication
    Tous et al Sci Adv. 2025 May 9;11(19):eadt1971. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    PhyA-Flp-397-Flp-396-FHY1
    Expression
    Mammalian
    Mutation
    None
    Available since
    May 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    233661-plasmid-map-sequence-id-463755

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Showing: 10241 - 10280 of 18759 results