We narrowed to 7,181 results for: RAP

1. AA062

   Plasmid

   #216019

   Purpose

   Fragmid fragment: (Cas protein) nickase Cas for prime editing

   Depositor

   John Doench

   Article

   McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

   Has Service

   Cloning Grade DNA

   Insert

   nCas9-SpG (H840A)_v1.1 [Sp]
   

   Use

   CRISPR; Fragment

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. AA060

   Plasmid

   #216017

   Purpose

   Fragmid fragment: (Cas protein) nickase Cas for prime editing

   Depositor

   John Doench

   Article

   McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

   Has Service

   Cloning Grade DNA

   Insert

   nCas9 (H840A)_v1.1 [Sp]
   

   Use

   CRISPR; Fragment

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. AA401

   Plasmid

   #215959

   Purpose

   Fragmid fragment: (guide cassette) guide expression

   Depositor

   John Doench

   Article

   McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

   Has Service

   Cloning Grade DNA

   Insert

   U6_v1; DR_v1 [Dj]; BsmBI_v1; BsmBI_v12
   

   Use

   CRISPR; Fragment

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. AA048

   Plasmid

   #216016

   Purpose

   Fragmid fragment: (Cas protein) deactivated Cas

   Depositor

   John Doench

   Article

   McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

   Has Service

   Cloning Grade DNA

   Insert

   dCas12a (D908A)_v1.1 [EnAs]
   

   Use

   CRISPR; Fragment

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pRDB_131

   Plasmid

   #216095

   Purpose

   Cas12a [EnAs] CRISPRa targeting CD97, CD4, CD26, CD274, positive control

   Depositor

   John Doench

   Article

   McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

   Insert

   CD97, CD4, CD26, CD274 guides
   

   Use

   CRISPR and Lentiviral; Positive controls

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pRDA_816

   Plasmid

   #216098

   Purpose

   CRISPRa, EF1a-driven ALFA-dCas12a-VP64 (Cas only)

   Depositor

   John Doench

   Article

   McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

   Insert

   Cas12a [EnAs]
   

   Use

   CRISPR and Lentiviral; Assembled vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pC3.1_CAG_oROS-Gr_LF(C199S)

   Plasmid

   #216114

   Purpose

   Expresses the loss-of-function version of the genetically encoded, ratiometric hydrogen peroxide sensor oROS-Gr in mamalian cells.

   Depositor

   Andre Berndt

   Article

   Lee et al bioRxiv 2024.01.31.578117

   Insert

   oROS-Gr_LF(C199S)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   April 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pcDNA5-FRT-TO-NUbo-E3(63)-mCherry-FLAG

   Plasmid

   #212809

   Purpose

   Constitutive or doxycycline-inducible expression of NUbo-E3(63)-mCherry-FLAG in mammalian cells

   Depositor

   Helle Ulrich

   Article

   Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

   Insert

   E3(63)
   

   Use

   Tags

   NUbo and mCherry-FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pcDNA5-FRT-TO-NUbo-E3(48)-P2A-3xFLAG-Ubc7

   Plasmid

   #212804

   Purpose

   Constitutive or doxycycline-inducible expression of NUbo-E3(48)-P2A-3xFLAG-Ubc7 in mammalian cells

   Depositor

   Helle Ulrich

   Article

   Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

   Insert

   NUbo-E3(48)-P2A-3xFLAG-Ubc7
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA5-FRT-TO-NUbo-E3(48)-mCherry-FLAG

    Plasmid

    #212803

    Purpose

    Constitutive or doxycycline-inducible expression of NUbo-E3(48)-mCherry-FLAG in mammalian cells

    Depositor

    Helle Ulrich

    Article

    Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

    Insert

    E3(48)
    

    Use

    Tags

    NUbo and mCherry-FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. YIp128-TET-NUbo-E3(48)-NLS-VSV

    Plasmid

    #212746

    Purpose

    Doxycycline-inducible expression of NUbo-E3(48)-NLS-VSV in budding yeast

    Depositor

    Helle Ulrich

    Article

    Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

    Insert

    E3(48)
    

    Use

    Integrative vector

    Tags

    NLS-VSV and NUbo

    Expression

    Yeast

    Mutation

    Promoter

    Tet

    Available since

    Feb. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. YIp128-TET-NUbo-E3(63)-NLS-VSV

    Plasmid

    #212756

    Purpose

    Doxycycline-inducible expression of NUbo-E3(63)-NLS-VSV in budding yeast

    Depositor

    Helle Ulrich

    Article

    Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

    Insert

    E3(63)
    

    Use

    Integrative vector

    Tags

    NLS-VSV and NUbo

    Expression

    Yeast

    Mutation

    Promoter

    Tet

    Available since

    Feb. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pcDNA5-FRT-TO-Myc-E3(1) C885A-CUbo

    Plasmid

    #212800

    Purpose

    Constitutive/Doxycycline-inducible expression of Myc-E3(1) C885A-CUbo in mammalian cells

    Depositor

    Helle Ulrich

    Article

    Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

    Insert

    E3(1)
    

    Use

    Tags

    CUbo and Myc

    Expression

    Mammalian

    Mutation

    HOIP(C885A)

    Promoter

    Available since

    Feb. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA5-FRT-TO-His8-H2B-CUbo(K63R)

    Plasmid

    #212814

    Purpose

    Constitutive or doxycycline-inducible expression of His8-H2B-CUbo(K63R) in mammalian cells

    Depositor

    Helle Ulrich

    Article

    Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

    Insert

    H2B
    

    Use

    Tags

    8xHis and CUbo(K63R)

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcDNA5-FRT-TO-NUbo-mCherry-FLAG

    Plasmid

    #212798

    Purpose

    Constitutive/Doxycycline-inducible expression of NUbo-mCherry-FLAG in mammalian cells

    Depositor

    Helle Ulrich

    Article

    Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

    Insert

    NUbo
    

    Use

    Tags

    mCherry-FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. YIp128-CUP1-His7-NUbo-GFP

    Plasmid

    #212791

    Purpose

    Copper-inducible expression of the artificial test substrate His7-NUbo-GFP in budding yeast

    Depositor

    Helle Ulrich

    Article

    Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

    Insert

    His7-NUbo-GFP
    

    Use

    Integrative vector

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    CUP1

    Available since

    Jan. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. YIp128-CUP1-His6-CUbo-GFP

    Plasmid

    #212792

    Purpose

    Copper-inducible expression of the artificial test substrate His6-CUbo-GFP in budding yeast

    Depositor

    Helle Ulrich

    Article

    Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

    Insert

    His6-CUbo-GFP
    

    Use

    Integrative vector

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    CUP1

    Available since

    Jan. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. YIp128-CUP1-CUbo-AID*-GFP

    Plasmid

    #212796

    Purpose

    Copper-inducible expression of the artificial test substrate His6-CUbo-AID*-GFP in budding yeast

    Depositor

    Helle Ulrich

    Article

    Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

    Insert

    CUbo-AID*-GFP
    

    Use

    Integrative vector

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    CUP1

    Available since

    Jan. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. YIp211-ADH-NUbo-E3(63)-mCherry-VSV

    Plasmid

    #212759

    Purpose

    Constitutive expression of NUbo-E3(63)-mCherry-VSV in budding yeast

    Depositor

    Helle Ulrich

    Article

    Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

    Insert

    E3(63)
    

    Use

    Integrative vector

    Tags

    NUbo and mCherry-VSV

    Expression

    Yeast

    Mutation

    Promoter

    ADH1

    Available since

    Jan. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. YIp128-ADH-NUbo-E3(63)-mCherry-VSV

    Plasmid

    #212758

    Purpose

    Constitutive expression of NUbo-E3(63)-mCherry-VSV in budding yeast

    Depositor

    Helle Ulrich

    Article

    Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

    Insert

    E3(63)
    

    Use

    Integrative vector

    Tags

    NUbo and mCherry-VSV

    Expression

    Yeast

    Mutation

    Promoter

    ADH1

    Available since

    Jan. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge