We narrowed to 6,078 results for: nls

1. Tad-CBE(ACT4)

   Plasmid

   #235060

   Purpose

   Expresses Tad-CBE(ACT4) in mammalian cells.

   Depositor

   Weixin Tang

   Article

   Wu et al Nat Biotechnol. 2025 Jul 7:10.1038/s4158

   Insert

   Tad-CBE(ACT4)-SpCas9 D10A
   

   Use

   CRISPR

   Tags

   2xUGI, SV40NLS and bpNLS

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. Tad-CBE(TCG2)

   Plasmid

   #235068

   Purpose

   Expresses Tad-CBE(TCG2) in mammalian cells.

   Depositor

   Weixin Tang

   Article

   Wu et al Nat Biotechnol. 2025 Jul 7:10.1038/s4158

   Insert

   Tad-CBE(TCG2)-SpCas9 D10A
   

   Use

   CRISPR

   Tags

   2xUGI, bpNLS and bpNLS

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. Tad-CBE(CCA3)

   Plasmid

   #235070

   Purpose

   Expresses Tad-CBE(CCA3) in mammalian cells.

   Depositor

   Weixin Tang

   Article

   Wu et al Nat Biotechnol. 2025 Jul 7:10.1038/s4158

   Insert

   Tad-CBE(CCA3)-SpCas9 D10A
   

   Use

   CRISPR

   Tags

   2xUGI, bpNLS and bpNLS

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. Tad-CBE(CCT2)

   Plasmid

   #235071

   Purpose

   Expresses Tad-CBE(CCT2) in mammalian cells.

   Depositor

   Weixin Tang

   Article

   Wu et al Nat Biotechnol. 2025 Jul 7:10.1038/s4158

   Insert

   Tad-CBE(CCT2)-SpCas9 D10A
   

   Use

   CRISPR

   Tags

   2xUGI, bpNLS and bpNLS

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. Tad-CBE(TCC2)

   Plasmid

   #235067

   Purpose

   Expresses Tad-CBE(TCC2) in mammalian cells.

   Depositor

   Weixin Tang

   Article

   Wu et al Nat Biotechnol. 2025 Jul 7:10.1038/s4158

   Insert

   Tad-CBE(TCC2)-SpCas9 D10A
   

   Use

   CRISPR

   Tags

   2xUGI, bpNLS and bpNLS

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. SpCas9 nickase for Split-PE (pJUL2497)

   Plasmid

   #190104

   Purpose

   Expresses nickase SpCas9(H840A) for Split-PE use

   Depositor

   Keith Joung

   Article

   Grunewald et al Nat Biotechnol. 2023 Mar;41(3):337-343.

   Insert

   nSpCas9
   

   Use

   Tags

   XTEN-bpNLS and bpNLS

   Expression

   Mammalian

   Mutation

   H840A

   Promoter

   CMV

   Available since

   Sept. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. ePPE-V223A

   Plasmid

   #205243

   Purpose

   For plant prime editing in wheat plants or monocotyledons protoplasts

   Depositor

   Zong Yuan

   Article

   Ni et al Genome Biol. 2023 Jun 29;24(1):156. doi:

   Insert

   SV40_NLS-nCas9-NC-MLV-SV40_NLS
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Detailed in manuscript

   Promoter

   Available since

   Sept. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. PE-P3-rice

   Plasmid

   #185612

   Purpose

   For plant prime editing in rice plants

   Depositor

   Jinxiao Yang

   Article

   Xu et al Nat Plants. 2022 Jan;8(1):45-52. doi: 10

   Insert

   3XFLAG-4XNLS-M-MLV-nCas9(H840A)-4XNLS-P2A-Hyg
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   ZmUbi

   Available since

   Aug. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. sABE v3.22_C

   Plasmid

   #209554

   Purpose

   pCMV-SV40_NLS-2xFKBP3-TadA-8e(77-167)-SpCas9(D10A)-SV40_NLS-BGHpA

   Depositor

   Xue Gao

   Article

   Zeng et al Nat Commun. 2023 Sep 11;14(1):5573. doi:

   Insert

   2xFKBP-TadA-8e(77-167)-SpCas9(D10A)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   pCMV

   Available since

   Nov. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. HiFi-nCas9-NG_P2A-mCherry

    Plasmid

    #219936

    Purpose

    Mammalian expression of Sp-HiFi-nCas9-NG with P2A-mCherry marker for use with MOBE systems

    Depositor

    Alexis Komor

    Article

    Cowan et al Nat Biotechnol. 2024 May 21. doi: 10.103

    Insert

    Sp-HiFi-nCas9(D10A)-NG_P2A-mCherry
    

    Use

    CRISPR

    Tags

    SV40 bpNLS and SV40 bpNLS-P2A-mCherry

    Expression

    Mammalian

    Mutation

    HiFi-nCas9(D10A/R691A)

    Promoter

    CMV

    Available since

    June 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. nCas9-SpRY_P2A-mCherry

    Plasmid

    #219935

    Purpose

    Mammalian expression of SpRY-nCas9 with P2A-mCherry marker for use with MOBE systems

    Depositor

    Alexis Komor

    Article

    Cowan et al Nat Biotechnol. 2024 May 21. doi: 10.103

    Insert

    Sp-nCas9(D10A)-SpRY_P2A-mCherry
    

    Use

    CRISPR

    Tags

    SV40 bpNLS and SV40 bpNLS-P2A-mCherry

    Expression

    Mammalian

    Mutation

    nCas9(D10A)

    Promoter

    CMV

    Available since

    June 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. RIB VP64 R1

    Plasmid

    #161493

    Purpose

    CRISPR/RNA scaffold based transcription regulation in Pichia pastoris BB3rN_pTEF2_dCAS9(3xFLAG_SV40NLS)_pPOR1_MS2bind_VP64_SV40NLS_pGAP_RIB1_R1_gRNA_MS2

    Depositor

    Diethard Mattanovich, Brigitte Gasser

    Article

    Baumschabl et al ACS Synth Biol. 2020 Dec 18;9(12):3202-3

    Inserts

    dCAS9
    MS2-VP64
    gRNA (Targeting R1 in the RIB1 promoter)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    pGAP, pPOR1, and pTEF2

    Available since

    March 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. S3_001_026_MV2

    Plasmid

    #134422

    Purpose

    pYTKMV2-pTDH3_NLS_FdeR-N_tPGK1-pGPM1-fdeO_mcherry_tTDH1

    Depositor

    Mattheos Koffas

    Article

    Wang et al ACS Synth Biol. 2019 Sep 20;8(9):2121-21

    Inserts

    mCherry
    NLS_FdeR-N
    

    Use

    Tags

    none

    Expression

    Yeast

    Mutation

    none

    Promoter

    pGMP1 and pTDH3

    Available since

    Dec. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. PE-P3-maize

    Plasmid

    #185611

    Purpose

    For plant prime editing in maize plants

    Depositor

    Jinxiao Yang

    Article

    Xu et al Nat Plants. 2022 Jan;8(1):45-52. doi: 10

    Insert

    3XFLAG-4XNLS-M-MLV-nCas9(H840A)-4XNLS
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    ZmUbi

    Available since

    Aug. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. Tol2_B-act_dCas9-Vp64-T2A-Citrine

    Plasmid

    #200248

    Purpose

    Zebrafish transgenics; Plasmid encodes dCas9-Vp64 and could be stably integrated in the zebrafish genome using Tol2 transgenesis

    Depositor

    Tatjana Sauka-Spengler

    Article

    Pelea et al bioRxiv 2023.05.08.539738

    Insert

    dCas9-Vp64-T2A-Citrine
    

    Use

    Zebrafish expression

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. THI VP64 pGAP T5

    Plasmid

    #161492

    Purpose

    CRISPR/RNA scaffold based transcription regulation in Pichia pastoris BB3rN_pTEF2_dCAS9(3xFLAG_SV40NLS)_pPOR1_MS2bind_VP64_SV40NLS_pGAP_THI11_T5_gRNA_MS2

    Depositor

    Diethard Mattanovich, Brigitte Gasser

    Article

    Baumschabl et al ACS Synth Biol. 2020 Dec 18;9(12):3202-3

    Inserts

    dCAS9
    MS2-VP64
    gRNA (Targeting T5 in the THI11 promoter)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    pGAP, pPOR1, and pTEF2

    Available since

    March 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. THI VP64 pGAP T1

    Plasmid

    #161488

    Purpose

    CRISPR/RNA scaffold based transcription regulation in Pichia pastoris BB3rN_pTEF2_dCAS9(3xFLAG_SV40NLS)_pPOR1_MS2bind_VP64_SV40NLS_pGAP_THI11_T1_gRNA_MS2

    Depositor

    Diethard Mattanovich, Brigitte Gasser

    Article

    Baumschabl et al ACS Synth Biol. 2020 Dec 18;9(12):3202-3

    Inserts

    dCAS9
    MS2-VP64
    gRNA (Targeting T1 in the THI11 promoter)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    pGAP, pPOR1, and pTEF2

    Available since

    March 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. S3_002_026_096

    Plasmid

    #134403

    Purpose

    pYTK096-pRPL18B_NLS_FdeR-N_tPGK1-pGPM1-fdeO_mcherry_tTDH1

    Depositor

    Mattheos Koffas

    Article

    Wang et al ACS Synth Biol. 2019 Sep 20;8(9):2121-21

    Inserts

    mCherry
    NLS_FdeR-N
    

    Use

    Tags

    None and none

    Expression

    Yeast

    Mutation

    None and none

    Promoter

    pGPM1 and pRPL18B

    Available since

    Dec. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. S3_004_026_MV1

    Plasmid

    #134416

    Purpose

    pYTKMV1-pTDH3_FdeR-N_2NLS_tPGK1-pGPM1-fdeO_mcherry_tTDH1

    Depositor

    Mattheos Koffas

    Article

    Wang et al ACS Synth Biol. 2019 Sep 20;8(9):2121-21

    Inserts

    mCherry
    FdeR-N_2xNLS
    

    Use

    Tags

    none

    Expression

    Yeast

    Mutation

    none

    Promoter

    pGMP1 and pTDH3

    Available since

    Dec. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. SpRY-Tad-CBE(CCG3)

    Plasmid

    #235083

    Purpose

    Expresses SpRY-Tad-CBE(CCG3) in mammalian cells.

    Depositor

    Weixin Tang

    Article

    Wu et al Nat Biotechnol. 2025 Jul 7:10.1038/s4158

    Insert

    Tad-CBE(CCG3)-SpRY Cas9 D10A
    

    Use

    CRISPR

    Tags

    2xUGI, bpNLS and bpNLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge