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G1397 DddAtox-N–SaKKH-Cas9(D10A)

Plasmid

#157839

Purpose

expresses split DddAtox-Cas9 construct in mammalian cells

Depositor

David Liu

Article

Mok et al Nature. 2020 Jul 8. pii: 10.1038/s41586-

Insert

G1397 DddAtox-N–SaKKH-Cas9(D10A)–UGI–SV40 NLS

Expression

Mammalian

Available Since

Aug. 4, 2020

Availability

Academic Institutions and Nonprofits only

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(K) tNOS (S)_T3 (SBE120)

Plasmid

#195010

Purpose

terminator from Agrobacterium tumefaciens nopaline synthase (tNOS, MF116010) for T3 position (K/S)

Depositor

Petri-Jaan Lahtvee

Article

Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

Insert

(K) tNOS (S)

Expression

Bacterial

Available Since

Feb. 28, 2023

Availability

Academic Institutions and Nonprofits only

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(H) tNOS (I) _T2 (SBE119)

Plasmid

#195006

Purpose

terminator from Agrobacterium tumefaciens nopaline synthase (tNOS, MF116010) for T2 position (H/I)

Depositor

Petri-Jaan Lahtvee

Article

Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

Insert

(H) tNOS (I)

Expression

Bacterial

Available Since

Feb. 28, 2023

Availability

Academic Institutions and Nonprofits only

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(B) HYG marker(R) (SBE264)

Plasmid

#195013

Purpose

hygromycin (HYG) resistance genes codon-optimized for R. toruloides and expressed under pXYL and tGPD

Depositor

Petri-Jaan Lahtvee

Article

Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

Insert

pXYL+HYG+tGPD

Expression

Bacterial

Promoter

pXYL

Available Since

Feb. 28, 2023

Availability

Academic Institutions and Nonprofits only

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(E) tNOS (F) _T1 (SBE118)

Plasmid

#194997

Purpose

terminator from Agrobacterium tumefaciens nopaline synthase (tNOS, MF116010) for T1 position (E/F)

Depositor

Petri-Jaan Lahtvee

Article

Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

Insert

(E) tNOS (F)

Expression

Bacterial

Available Since

Feb. 28, 2023

Availability

Academic Institutions and Nonprofits only

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(B) BLE marker(R) (SBE262)

Plasmid

#195012

Purpose

bleomycin (BLE) resistance genes codon-optimized for R. toruloides and expressed under pXYL and tGPD

Depositor

Petri-Jaan Lahtvee

Article

Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

Insert

pXYL+BLE+tGPD

Expression

Bacterial

Promoter

pXYL

Available Since

Feb. 28, 2023

Availability

Academic Institutions and Nonprofits only

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pORANGE Cacnb4-GFP KI

Plasmid

#139663

Purpose

Endogenous tagging of CaVβ4: C-terminal (amino acid position: H417)

Depositor

Harold MacGillavry

Article

Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

Insert

gRNA and GFP donor

Expression

Mammalian

Promoter

U6 and Cbh

Available Since

April 17, 2020

Availability

Academic Institutions and Nonprofits only

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pORANGE GFP-Cacnb1 KI

Plasmid

#139660

Purpose

Endogenous tagging of CaVβ1: N-terminal (amino acid position: S8)

Depositor

Harold MacGillavry

Article

Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

Insert

gRNA and GFP donor

Expression

Mammalian

Promoter

U6 and Cbh

Available Since

April 17, 2020

Availability

Academic Institutions and Nonprofits only

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pNarsenic_noProm

Plasmid

#247321

Purpose

Control plasmid for pNarsenic lacking the ArsR-regulated promoter upstream of the reporter gene

Depositor

Eveline Peeters

Article

Crabbe et al Synth Biol (Oxf). 2026 Jan 10;11(1):ysag

Inserts

fdeR
mkate2
Arsenical resistance operon repressor

Use

Synthetic Biology

Promoter

None, P_fdeA, and P_fdeR

Available Since

March 10, 2026

Availability

Academic Institutions and Nonprofits only

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(R)pGPD1(D)_P1(SBE109)

Plasmid

#194993

Purpose

promoter of the gene glyceraldehyde-3-phosphate dehydrogenase from R. toruloides for a P1 position (overhangs R/D)

Depositor

Petri-Jaan Lahtvee

Article

Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

Insert

(R) pGPD (D)

Expression

Bacterial

Available Since

Feb. 26, 2024

Availability

Academic Institutions and Nonprofits only

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