We narrowed to 9,397 results for: bli

1. (915) SB EF1a dCasphi-[myc-ZIM3-HA] U6-gStop

   Plasmid

   #195623

   Purpose

   Sleeping Beauty EF1a plasmid for constitutive expression of dCasphi-ZIM3 CRISPRi construct

   Depositor

   Tonia Rex

   Insert

   dCasphi
   

   Tags

   C-Myc (NLS), HA tag, and ZIM3

   Expression

   Mammalian

   Mutation

   P749A and P753A

   Promoter

   EF1a (Mlu1/EcoR1)

   Available Since

   July 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSR23: pET-SUMO-USP21 (1-565)

   Plasmid

   #240189

   Purpose

   Bacterial expression for USP21 (1-565)

   Depositor

   Cynthia Wolberger

   Insert

   USP21 (USP21 Human)
   

   Tags

   6x-His-SUMO

   Expression

   Bacterial

   Available Since

   June 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pQdCas12a.sggfp(A)

   Plasmid

   #236038

   Purpose

   The plasmid pQdCas12a.sggfp(A) expresses the dCas12a endonuclease and the sgRNA (design A) targeting the gfp gene.

   Depositor

   Radhakrishnan Mahadevan

   Insert

   quorum sensing cassette luxI, luxR, and the LuxR-dependent promoter pLuxI , dCas12a and sgRNA design (A) of gfp gene
   

   Use

   CRISPR

   Promoter

   quorum sensing promoter

   Available Since

   April 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pQdCas12a.sggfp(B)

   Plasmid

   #236040

   Purpose

   The plasmid pQdCas12a.sggfp(B) expresses the dCas12a endonuclease and the sgRNA (design B) targeting the gfp gene.

   Depositor

   Radhakrishnan Mahadevan

   Insert

   quorum sensing cassette luxI, mutated luxR, and the LuxR-dependent promoter pLuxI , dCas12a and sgRNA design (B) of gfp gene
   

   Use

   CRISPR

   Promoter

   quorum sensing promoter

   Available Since

   April 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRDA_852

   Plasmid

   #185527

   Purpose

   Lentiviral vector that enables Cre-mediated expression of two sgRNAs. Also encodes the fluorophore violet-excited GFP (Vex) as a marker of transduction.

   Depositor

   Arlene Sharpe

   Inserts

   First sgRNA cassette
   Second sgRNA cassette
   

   Use

   CRISPR, Cre/Lox, and Lentiviral ; Flp/frt

   Expression

   Mammalian

   Promoter

   Human U6 and Mouse U6

   Available Since

   April 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pdCas9-tdTomato

   Plasmid

   #232631

   Purpose

   catalytically inactive dCas9 for localization; labeled with tdTomato

   Depositor

   Karsten Rippe

   Insert

   dCas9-tdTomato
   

   Use

   CRISPR

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   none

   Promoter

   CMV (TATA box removed)

   Available Since

   Feb. 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. 1996_pCCLsin.PPT.hPGK.EGFP-mCEP83.PRE

   Plasmid

   #231805

   Purpose

   Label the mother centrosome-specific distal appendage protein CEP83 by electroporating an mRNA encoding it as well as an eGFP fusion protein.

   Depositor

   Annette Oxenius

   Insert

   eGFP-mCEP83
   

   Use

   Lentiviral

   Tags

   eGFP

   Expression

   Mammalian

   Available Since

   Feb. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLenti puro HA-STX3

   Plasmid

   #227590

   Purpose

   Lentiviral plasmid expressing human STX3 protein

   Depositor

   Melina Fan, Addgene Research Program

   Insert

   STX3 (STX3 Human)
   

   Use

   Lentiviral

   Tags

   HA

   Promoter

   CMV

   Available Since

   Jan. 22, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pX330-MM002-ZFP143-C-terminal-sgRNA

   Plasmid

   #212706

   Purpose

   Plasmid for transient expression of pspCas9(BB)-2A-Puro nuclease and sgRNA targetting the C terminal of Zfp143 locus

   Depositor

   Elzo de Wit

   Insert

   ZFP143 C-terminal sgRNA (Zfp143 Mouse)
   

   Use

   CRISPR

   Available Since

   Dec. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pENTR/hBIG1

    Plasmid

    #226251

    Purpose

    Entry plasmid of hBIG1 for Gateway system

    Depositor

    Hye-Won Shin

    Insert

    BIG1 (ARFGEF1 Human)
    

    Use

    Entry vector

    Available Since

    Nov. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pENTR/HA-hBIG2

    Plasmid

    #226252

    Purpose

    Entry plasmid of N-terminally HA-tagged hBIG2 for Gateway system

    Depositor

    Hye-Won Shin

    Insert

    BIG2 (ARFGEF2 Human)
    

    Use

    Entry vector

    Tags

    HA

    Available Since

    Nov. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLenti puro HA-VIM

    Plasmid

    #227585

    Purpose

    Lentiviral plasmid expressing human VIM protein

    Depositor

    Melina Fan, Addgene Research Program

    Insert

    VIM (VIM Human)
    

    Use

    Lentiviral

    Tags

    HA

    Promoter

    CMV

    Available Since

    Nov. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEN_TT DNM1L-V5

    Plasmid

    #220359

    Purpose

    Gateway entry vector for an inducible V5-tagged DNM1L

    Depositor

    Kevin Janes

    Insert

    DNM1L (DNM1L Human)
    

    Use

    Entry vector for gateway cloning

    Tags

    V5

    Available Since

    Oct. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCDNA3.SLC17A2-flag

    Plasmid

    #224419

    Purpose

    Expresses SLC17A2 in mammalian cells

    Depositor

    Jonathan Long

    Insert

    Solute carrier family 17 (sodium phosphate), member 2 (SLC17A2 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. CD55-Delta3

    Plasmid

    #220872

    Purpose

    Expresses CD55 variant in mammalian cells

    Depositor

    Ferenc Scheeren

    Insert

    CD55 Delta-3
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Delta-3, short consensus repeat (SCR) domains 1, …

    Promoter

    CMV

    Available Since

    Aug. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. CD55-Delta4

    Plasmid

    #220873

    Purpose

    Expresses CD55 variant in mammalian cells

    Depositor

    Ferenc Scheeren

    Insert

    CD55 Delta-4
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Delta-4, short consensus repeat (SCR) domains 1, …

    Promoter

    CMV

    Available Since

    Aug. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. CD55-Delta1

    Plasmid

    #220870

    Purpose

    Expresses CD55 variant in mammalian cells

    Depositor

    Ferenc Scheeren

    Insert

    CD55 Delta-1
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Delta-1, the first short consensus repeat (SCR) d…

    Promoter

    CMV

    Available Since

    Aug. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. CD55-Delta2

    Plasmid

    #220871

    Purpose

    Expresses CD55 variant in mammalian cells

    Depositor

    Ferenc Scheeren

    Insert

    CD55 Delta-2
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Delta-2, short consensus repeat (SCR) domains 1 a…

    Promoter

    CMV

    Available Since

    Aug. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. hs-cre::EBD, UAS-dBrainbow attB

    Plasmid

    #220326

    Purpose

    expresses a heat-shock inducible cre::EBD fusion protein and UAS-dBrainbow

    Depositor

    Erdem Bangi

    Inserts

    Cre::EBD (with intron)
    dBrainbow
    

    Expression

    Insect

    Promoter

    UAS and hs

    Available Since

    Aug. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pYES2/NTA/Leu-LegA7Δank

    Plasmid

    #216519

    Purpose

    Expression of LegA7 ΔAnk

    Depositor

    Ralph Isberg

    Insert

    LegA7 ΔAnk
    

    Tags

    HIS-tag

    Expression

    Bacterial and Yeast

    Mutation

    LegA7 missing aa 331-454

    Available Since

    June 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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