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Plasmid#213405PurposePeroxidase (APEX) reporter for TEV protease cleavageDepositorInsertE5-TEVcs-APEX(201-250)-K5-EGFP-APEX(2-200)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEPQD0CM0281
Plasmid#162302PurposeLevel 0 N-terminal tag part for MoClo assembly, CCAT-AATGDepositorInsertN-terminal tag, [S-tag]-[TEV cleavage site]
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
CXXC1
Plasmid#32045PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertCXXC1 (CXXC1 Human)
UseTagsHis (with TEV cleavage)ExpressionBacterialMutationCXXC domain (amino acids residues 161-222)PromoterAvailable sinceJan. 3, 2012AvailabilityIndustry, Academic Institutions, and Nonprofits -
pJExpress414_GmPOPB-S577A
Plasmid#92235PurposeExpress S577A mutant of prolyl oligopeptidase from Galerina marginataDepositorInsertProlyl oligopeptidase B from Galerina marginata-S577A mutant
UseTags6x-Histag, TEV cleavage siteExpressionBacterialMutationSerine 577 to AlaninePromoterT7 promoterAvailable sinceAug. 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGC4780
Plasmid#113408PurposeTranslation reporter with inducible translation coupling (high native GFP expression)DepositorInsert158_33121123_1
UseSynthetic BiologyTagsfused to codon deptimized sfGFP with Flexible lin…ExpressionBacterialMutationPromoterAvailable sinceApril 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMH-HT_RcCHAD
Plasmid#127782PurposeExpresses RcCHAD in E. coli cellsDepositorInsertRcCHAD
UseTags6xHis tag + TEV cleavage siteExpressionBacterialMutationPromoterAvailable sinceAug. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGC4785
Plasmid#113409PurposeTranslation reporter with inducible translation coupling (low native GFP expression, high under coupling)DepositorInsert359_31121113_2
UseSynthetic BiologyTagsfused to codon deptimized sfGFP with Flexible lin…ExpressionBacterialMutationPromoterAvailable sinceApril 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJExpress414_GmPOPB-D661A
Plasmid#92236PurposeExpress D661A mutant of prolyl oligopeptidase from Galerina marginataDepositorInsertProlyl oligopeptidase B from Galerina marginata-D661A mutant
UseTags6x-Histag, TEV cleavage siteExpressionBacterialMutationAspartate 661 to AlaninePromoterT7 promoterAvailable sinceAug. 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
wt Cus2p
Plasmid#73046PurposeWild-type S. cerevisiae Cus2p gene for recombinant expression and crystallizationDepositorArticleInsertCUS2p (CUS2 Budding Yeast)
UseTags6x His tag fused to TEV cleavage siteExpressionBacterial and YeastMutationPromoterT7Available sinceMay 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEG608
Plasmid#40194DepositorInsertMex-5 (RNAi resistant) (mex-5 Nematode)
UseTags3xFLAG (DYKDHDGDYKDHDIDYKDDDDK), GFP, and TEV pro…ExpressionWormMutationExon 2 recoded to be RNAi-resistantPromoter4.4 kb mex-5 promoter fragmentAvailable sinceSept. 19, 2012AvailabilityAcademic Institutions and Nonprofits only -
pAG415Gal_Gcn4 E3GFP
Plasmid#233008PurposeGalactose iduced expression of Gcn4 E+GFP in yeastDepositorInsertGcn4 E+
UseTagsHA-GFP-HA and TEV cleavage siteExpressionYeastMutationD105E, D118E, D139EPromoterGal1Available sinceMarch 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAG415Gal_Gcn4 F124IHA
Plasmid#232993PurposeGalactose iduced expression of Gcn4 F124IHA in yeastDepositorInsertGcn4 F124I
UseTags3xHA and TEV cleavage siteExpressionYeastMutationF124IPromoterGal1Available sinceMarch 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAG415Gal_Gcn4 WTSLFD
Plasmid#232980PurposeGalactose iduced expression of Gcn4 WTSLFD+ in yeastDepositorInsertGcn4 WTSLFD+
UseTags3xHA and TEV cleavage siteExpressionYeastMutationF108W, E109T, Y110S, E111L, N112F, L113DPromoterGal1Available sinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAG415Gal_Gcn4 Aro2+
Plasmid#232982PurposeGalactose iduced expression of Gcn4 Aro2+ in yeastDepositorInsertGcn4 Aro2+
UseTagsTEV cleavage siteExpressionYeastMutationN116F, S117E, E119N, P129Q, T132YPromoterGal1Available sinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAG415Gal_Gcn4 Aro3+
Plasmid#232983PurposeGalactose iduced expression of Gcn4 Aro3+ in yeastDepositorInsertGcn4 Aro3+
UseTagsTEV cleavage siteExpressionYeastMutationD103Y, N112T, L113Y, S117F, K140RPromoterGal1Available sinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAG415Gal_Gcn4 W120AHA
Plasmid#232992PurposeGalactose iduced expression of Gcn4 W120AHA in yeastDepositorInsertGcn4 W120A
UseTags3xHA and TEV cleavage siteExpressionYeastMutationW120APromoterGal1Available sinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAG415Gal_Gcn4 Aro3+HA
Plasmid#232990PurposeGalactose iduced expression of Gcn4 Aro3+HA in yeastDepositorInsertGcn4 Aro3+
UseTags3xHA and TEV cleavage siteExpressionYeastMutationD103Y, N112T, L113Y, S117F, K140RPromoterGal1Available sinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAG415Gal_Gcn4 WT 44merHA
Plasmid#232988PurposeGalactose iduced expression of Gcn4 WT 44merHA in yeastDepositorInsertGcn4 WT 44mer
UseTags3xHA and TEV cleavage siteExpressionYeastMutationPromoterGal1Available sinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAG415Gal_Gcn4 Aro2+HA
Plasmid#232989PurposeGalactose iduced expression of Gcn4 Aro2+HA in yeastDepositorInsertGcn4 Aro2+
UseTags3xHA and TEV cleavage siteExpressionYeastMutationN116F, S117E, E119N, P129Q, T132YPromoterGal1Available sinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAG415Gal_Gcn4 F124IGFP
Plasmid#233005PurposeGalactose iduced expression of Gcn4 F124IGFP in yeastDepositorInsertGcn4 F124I
UseTagsHA-GFP-HA and TEV cleavage siteExpressionYeastMutationF124IPromoterGal1Available sinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only