We narrowed to 1,479 results for: pbluescript
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Plasmid#184395PurposeDonor plasmid for stable integration of firefly luciferase control reporter at AAVS1DepositorInsertFirefly luciferase beta-globin
UseCRISPR; Donor plasmid for hdrTags3X FLAGExpressionMammalianMutationPromoterTet-On 3GAvailable sinceMay 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pROSA26-promoter
Plasmid#21710DepositorInsertROSA26 promoter (Gt(ROSA)26Sor Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 28, 2009AvailabilityAcademic Institutions and Nonprofits only -
pBluescriptSKII+ U6-sgRNA(F+E) ACTB
Plasmid#74705PurposeEncodes an sgRNA for spCas9 driven by hU6 promoter with a modified scaffold (Chen et al. Cell 2013) and a spacer targeting the 3'UTR of ACTB mRNADepositorInsertU6 promoter driving sgRNA targeting the 3'UTR of ACTB mRNA
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceApril 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-TetOn-3XFLAG-renilla-beta-globin-control-AAVS1
Plasmid#184396PurposeDonor plasmid for stable integration of Renilla luciferase control reporter at AAVS1DepositorInsertRenilla luciferase beta-globin
UseCRISPR; Donor plasmid for hdrTags3X FLAGExpressionMammalianMutationPromoterTet-On 3GAvailable sinceMay 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT-GEM(0)
Plasmid#62891PurposeTrojan-Gal4 Expression Module in Phase 0. Insert T2A-Gal4 to genomic loci using the Crispr/Cas technology. Can be converted by cassette exchange into other Trojan exonsDepositorTypeEmpty backboneUseTagsExpressionMutationPromoterAvailable sinceJuly 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBS human IRS-1
Plasmid#11359DepositorInsertIRS-1 (IRS1 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 1, 2006AvailabilityAcademic Institutions and Nonprofits only -
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pBS mouse IRS-2
Plasmid#11372DepositorInsertIRS-2 (Irs2 Mouse)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 1, 2006AvailabilityAcademic Institutions and Nonprofits only -
pBSSK p85 alpha (delta iSH2)
Plasmid#13432DepositorInsertp85 alpha (delta iSH2) (PIK3R1 Human)
UseTagsflagExpressionBacterialMutationDominant negative p85 alpha. The p110 alpha bindi…PromoterAvailable sinceNov. 1, 2006AvailabilityAcademic Institutions and Nonprofits only -
pBluescriptSKII+ U6-sgRNA(F+E) TFRC
Plasmid#74709PurposeEncodes an sgRNA for spCas9 driven by hU6 promoter with a modified scaffold (Chen et al. Cell 2013) and a spacer targeting the 3'UTR of TFRC mRNADepositorInsertU6 promoter driving sgRNA targeting the 3'UTR of TFRC mRNA
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceApril 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHDAdVenus-geo-TK
Plasmid#26423DepositorInsertPGK-b-geo, HSV-TK, CAG-Venus
UseAdenoviralTagsExpressionMammalianMutationPromoterAvailable sinceMarch 31, 2011AvailabilityAcademic Institutions and Nonprofits only -
pBS-AC3CtermGenomic-mCherry
Plasmid#105250Purposemouse mCherry (with STOP and extra-sequence) with homology arms for Adcy3DepositorInsertmCherry (with STOP and extra-sequence) with homology arms for mouse Adcy3 (Adcy3 Mouse)
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
BAT-gal
Plasmid#20889DepositorInsert7XTCF binding sites, Siamois minimal promoter, NLS-lacz
UseMouse TargetingTagsExpressionMammalianMutation7 multimerized TCF-binding elements cloned upstre…PromoterAvailable sinceMay 7, 2009AvailabilityAcademic Institutions and Nonprofits only -
tGFP
Plasmid#26864DepositorInsertTruncated GFP-IRES-Puro
UseTagsExpressionBacterialMutationDeletion of nt 1-36PromoterAvailable sinceNov. 19, 2010AvailabilityAcademic Institutions and Nonprofits only -
2-Luc-Del-50
Plasmid#21730DepositorInsertp21 promoter deletion mutant (CDKN1A Human)
UseLuciferaseTagsluciferaseExpressionMammalianMutationIdentical to 2-Luc except that the 50-bp region b…PromoterAvailable sinceAug. 10, 2012AvailabilityAcademic Institutions and Nonprofits only -
3269 pBS Bcl-2
Plasmid#8764DepositorInsertBcl-2 (Bcl2 Mouse)
UseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 6, 2005AvailabilityAcademic Institutions and Nonprofits only -
pBS-Squ:mCherry
Plasmid#20163DepositorInsertspaghetti squash (squ, myosin II RLC) 5' UTR and ORF fused to mCherry followed by squ 3' UTR (sqh Fly)
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 14, 2009AvailabilityAcademic Institutions and Nonprofits only -
pCA-T-intron(Neo)-G(LNL)
Plasmid#36908DepositorInsertsT (i.e., ATG, start codon as a first exon)
GFP
beta-globin intron
Neo
UseTagsExpressionMammalianMutationdeleted nucleotides before nucleotide 275 and ins…PromoterCAG (chicken beta actin promoter and CMV enhancer…Available sinceAug. 10, 2012AvailabilityAcademic Institutions and Nonprofits only -
pBSSK p85 alpha (delta SH3)
Plasmid#13430DepositorInsertp85 alpha (delta SH3) (PIK3R1 Human)
UseTagsFLAGExpressionBacterialMutationSH3 domain replaced by flag tag.PromoterAvailable sinceNov. 1, 2006AvailabilityAcademic Institutions and Nonprofits only -
pBSSK p85 alpha (delta BH)
Plasmid#13431DepositorInsertp85 alpha (delta BH) (PIK3R1 Human)
UseTagsFLAGExpressionBacterialMutationBH domain replaced by flag tag.PromoterAvailable sinceNov. 1, 2006AvailabilityAcademic Institutions and Nonprofits only -
y2
Plasmid#53586PurposeProduces an active Arabidopsis thaliana lycopene epsilon-cyclase in E. coli. Use with pAC-LYC or pAC-LYCipi to make delta-carotene in E. coli.DepositorInsertlcyE (LUT2 Mustard Weed)
UseTagsExpressionMutationPromoterAvailable sinceJan. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHA843 - hrp-1p::hrp-1HsLCdeltaLC
Plasmid#139200PurposeExpresses hrp-1p::hrp-1HsLCdeltaLC::hrp-1 3'UTRDepositorInserthrp-1 (hrp-1 Nematode)
UseTagsExpressionWormMutationstop at beginning of last exonPromoterAvailable sinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
11.5kb-GFP (pBSK)
Plasmid#29459DepositorInsertSHIP-1 promoter (Inpp5d Mouse)
UseTagsGFPExpressionBacterialMutationPromoterAvailable sinceMarch 31, 2011AvailabilityAcademic Institutions and Nonprofits only -
pBT267_(pCA-ATG-intron-tTA2-TRE-tdT3Myc)
Plasmid#36879DepositorInsertstTA2
tdT-3Myc
UseTags3 Myc tagsExpressionMammalianMutationinsertion of a beta-globin intron with a loxP sit…PromoterCAG (chicken beta actin promoter and CMV enhancer…Available sinceAug. 8, 2012AvailabilityAcademic Institutions and Nonprofits only -
pROSA26-G19
Plasmid#21711DepositorInsertROSA26 G19 (Gt(ROSA)26Sor Mouse)
UseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 28, 2009AvailabilityAcademic Institutions and Nonprofits only -
pCA-T-intron(Neo)-G(FLLFLNL)
Plasmid#40021DepositorInsertsT (ATG)
GFP
beta-globin intron
Neo
UseTagsExpressionMutationdeleted nucleotides 1-274 and insertion of FRT, …PromoterCAG (chicken beta globin promoter and CMV enhance…Available sinceOct. 12, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCRW0.8 p21 (rat)
Plasmid#16477DepositorInsertp21 (Cdkn1a Rat)
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 7, 2008AvailabilityAcademic Institutions and Nonprofits only -
pBS mouse IRS4 promoter
Plasmid#11445DepositorInsertIRS-4 promoter (Irs4 Mouse)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 3, 2006AvailabilityAcademic Institutions and Nonprofits only -
pBT310_pBS-U-attP-FRT5-pSV40-Neo-pA-pVasa-PhiC31o-pA-FRT5
Plasmid#52543PurposeExpression of FRT5-flanked Neo; homologous recombination targeting vector cloningDepositorInsertsPhiC31o attP (full length)
Neo
PhiC31o integrase
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
3186 pBS Bcl-XL Exon 1
Plasmid#8755DepositorInsertBcl-XL (Exon 1) (Bcl2l1 Mouse)
UseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 6, 2005AvailabilityAcademic Institutions and Nonprofits only -
pBS-miR106a-363
Plasmid#19028DepositorInsertmicroRNA 106a-363 (Mir106a Mouse)
UseMouse TargetingTagsExpressionMutationPromoterAvailable sinceAug. 25, 2008AvailabilityAcademic Institutions and Nonprofits only -
pBS-AMV-β1L9'S-nAchR
Plasmid#20685DepositorInsertnicotinic acetylcholine receptors (Chrnb1 Mouse)
UseTagsAMV and pA 50ExpressionBacterialMutationLeucine at 9' position in M2 region changed …PromoterAvailable sinceApril 3, 2009AvailabilityAcademic Institutions and Nonprofits only -
pBT307_pBS-U-attPx3-FRT5-pSV40-Neo-pA-PhiC31o-pA-FRT5
Plasmid#52540PurposeExpression of FRT5-flanked Neo; homologous recombination targeting vector cloningDepositorInsertsPhiC31o attPx3 (70 bp each)
Neo
PhiC31o integrase
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBT309a_pBS-U-attPx3-FRT5-pSV40-Neo-pA-pVasa-PhiC31o-pA-FRT5
Plasmid#52542PurposeExpression of FRT5-flanked Neo; homologous recombination targeting vector cloningDepositorInsertsPhiC31o attPx3 (70 bp each)
Neo
PhiC31o integrase
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBlue-hFX(AA88)-HA
Plasmid#15402DepositorInserthuman frataxin cDNA (FXN Human)
UseTagsAU1, HA, and HISExpressionBacterialMutationcontains cDNA coding sequence for amino acids 88-…PromoterAvailable sinceAug. 17, 2007AvailabilityAcademic Institutions and Nonprofits only -
pMSIP124: pDonor(CyCAST)_0.5kb-cargo
Plasmid#200848PurposeCyCAST donor harboring non-functional cargo.DepositorInsertCyCAST
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
JD179
Plasmid#229496PurposeAdenoviral helper plasmid containing inducible E2A and L4-22k/33k genes and constitutive VA RNA I geneDepositorInsertE2A, L4-22k/33k, VA RNA I
UseAAV and AdenoviralTagsExpressionMammalianMutationPromoterTre (E2A, L4-22k/33k). Wt promoter (VA RNA I), ET…Available sinceDec. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHR-LS
Plasmid#205438PurposeExpression of Gaussia luciferase (gLuc) and spectinomycin resistance (Spec) genesDepositorInsertsGaussia luciferase gene
spectinomycin resistance gene
UseCre/Lox and Luciferase; Chlamydomonas expressionTagsExpressionMutationPromoterHeat shock protein 70A (HSP70A)/ribulose bisphosp…Available sinceAug. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBS-V9
Plasmid#173797PurposepBS plasmid containing aadA gene with 16S rRNA promoter and psbA 3’ region, Silene noctiflora ClpP1 gene with the tobacco regulatory elements and flanking regions for tobacco plastome transformationDepositorInsertsaadA
ClpP1
UseTagsExpressionBacterial and PlantMutationPromoterAvailable sinceSept. 15, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pHA841 - hrp-1p::hrp-1HsLCWT
Plasmid#139198PurposeExpresses hrp-1p::hrp-1HsLCWT::hrp-1 3'UTRDepositorInserthrp-1 (hrp-1 Nematode)
UseTagsExpressionWormMutationlast exon replaced with correspoding human sequen…PromoterAvailable sinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHA842 - hrp-1p::hrp-1HsLCD290V
Plasmid#139199PurposeExpresses hrp-1p::hrp-1HsLCD290V::hrp-1 3'UTRDepositorInserthrp-1 (hrp-1 Nematode)
UseTagsExpressionWormMutationD290V, last exon replaced with correspoding human…PromoterAvailable sinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHA847 - mec-4p::hrp-1mScarlet
Plasmid#139201PurposeExpresses mScarlet tagged hrp-1 in mec-4 neuronsDepositorInserthrp-1 (hrp-1 Nematode)
UseTagsExpressionWormMutationmScarlet added between RRMs and LCPromoterAvailable sinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHA848 - mec-4p::hrp-1HsLCWTmScarlet
Plasmid#139202PurposeExpresses mScarlet tagged hrp-1HsLCWT in mec-4 neuronsDepositorInserthrp-1 (hrp-1 Nematode)
UseTagsExpressionWormMutationmScarlet added between RRMs and LC, last exon rep…PromoterAvailable sinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHA849 - mec-4p::hrp-1HsLCD290VmScarlet
Plasmid#139203PurposeExpresses mScarlet tagged hrp-1HsLCD290V in mec-4 neuronsDepositorInserthrp-1 (hrp-1 Nematode)
UseTagsExpressionWormMutationmScarlet added between RRMs and LC, D290V, last e…PromoterAvailable sinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHA845 - hrp-1p::hrp-1HsLCWTmScarlet
Plasmid#139205PurposeExpresses mScarlet tagged hrp-1HsLCWTDepositorInserthrp-1 (hrp-1 Nematode)
UseTagsExpressionWormMutationmScarlet added between RRMs and LC, last exon rep…PromoterAvailable sinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHA846 - hrp-1p::hrp-1HsLCD290VmScarlet
Plasmid#139206PurposeExpresses mScarlet tagged hrp-1HsLCD290VDepositorInserthrp-1 (hrp-1 Nematode)
UseTagsExpressionWormMutationmScarlet added between RRMs and LC, D290V, last e…PromoterAvailable sinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
Met138ILeu ELL2
Plasmid#127260PurposeFor in vitro translation of human ELL2 with Met138 mutated to Ileu to ask if there is internal initiationDepositorInsertELL2 (Met138 to Ileu) (ELL2 Human)
UseIn vitro translationTagsExpressionMutationMet138 to Ileu QuikchangePromoterT7Available sinceJuly 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
Met186ILeu ELL2
Plasmid#127261PurposeFor in vitro translation of human ELL2 with Met186 mutated to Ileu to ask if there is internal initiationDepositorInsertELL2 (Met186 to Ileu) (ELL2 Human)
UseIn vitro translationTagsExpressionMutationMet186 to Ileu QuikchangePromoterT7Available sinceJuly 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
Met138ILeu, Met186ILeu ELL2
Plasmid#127265PurposeFor in vitro translation of human ELL2 with Met138ILeu, Met186ILeuDepositorInsertELL2 (Met138ILeu, Met186ILeu) (ELL2 Human)
UseIn vitro translationTagsExpressionMutationTwo Mets (138,186) to ILeu QuikchangePromoterT7Available sinceJuly 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
Met133ILeu ELL2
Plasmid#127259PurposeFor in vitro translation of human ELL2 with Met133 mutated to Ileu to ask if there is internal initiationDepositorInsertELL2 (Met133 to Ileu) (ELL2 Human)
UseIn vitro translationTagsExpressionMutationMet133 to Ileu QuikchangePromoterT7Available sinceJuly 12, 2019AvailabilityAcademic Institutions and Nonprofits only