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Showing: 501 - 520 of 5682 results

  1. pSECRETS-A

    Plasmid
    #196986
    Purpose
    For SECRETS protocol to screen for gRNA activity and specificity: bacterial expression Cas9 in the presence of anhydrotetracycline (aTc).
    Depositor
    Eric Josephs
    Article
    Herring-Nicholas et al Commun Biol. 2024 Jan 12;7(1):86. doi: 1
    Insert
    S. pyogenes Cas9
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    pltetO1
    Available since
    Jan. 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    196986-plasmid-map-sequence-id-396953

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  2. pSecEffector

    Plasmid
    #172891
    Purpose
    Contains TEVp display cassette, atc inducible (in any E. coli strain) BxbI and arabinose inducible TP901
    Depositor
    Urartu Seker
    Article
    Ahan et al ACS Synth Biol. 2019 Apr 19;8(4):686-696
    Inserts
    Bxb1
    TP901
    TEV Display cassette
    ProD promoter between anti aligned Bxb1 sites
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    ProD, nTETO, and pBAD /AraC
    Available since
    July 29, 2022
    Availability
    Academic Institutions and Nonprofits only
    172891-plasmid-map-sequence-id-368224

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  3. pMD19T-slr0230-PL31-sgRNANT1-KmR

    Plasmid
    #73221
    Purpose
    Contains sgRNA which targets GFPmut3b. Under an aTc inducible promoter. Suicide vector inserts into slr0230 site of Synechocystis. Carries kanamycin resistance. Propogates in E. coli
    Depositor
    Paul Hudson
    Article
    Yao et al ACS Synth Biol. 2015 Dec 28.
    Insert
    PL31-sgRNA NT1
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PL31
    Available since
    Feb. 23, 2016
    Availability
    Academic Institutions and Nonprofits only
    73221-plasmid-map-sequence-id-135054

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  7. pBW015

    Plasmid
    #131819
    Purpose
    Vector with aTc inducible M. laminosus Fd1 mutant with the estrogen recepor-ligand binding domain fused between amino acids 55 and 56 with a C-terminal fused mRFP1
    Depositor
    Jonathan Silberg
    Article
    Wu et al bioRxiv May 23, 2019
    Insert
    M. laminosus Fd1 mutant with the estrogen recepor-ligand binding domain fused between amino acids 55 and 56
    Use
    Synthetic Biology
    Tags
    mRFP1
    Expression
    Mutation
    Promoter
    Available since
    Oct. 9, 2019
    Availability
    Academic Institutions and Nonprofits only
    131819-plasmid-map-sequence-id-259074

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  8. pBW013

    Plasmid
    #131816
    Purpose
    Vector with aTc inducible M. laminosus Fd1 mutant with the estrogen recepor-ligand binding domain fused between amino acids 54 and 55 with a C-terminal fused mRFP1
    Depositor
    Jonathan Silberg
    Article
    Wu et al bioRxiv May 23, 2019
    Insert
    M. laminosus Fd1 mutant with the estrogen recepor-ligand binding domain fused between amino acids 54 and 55
    Use
    Synthetic Biology
    Tags
    mRFP1
    Expression
    Mutation
    Promoter
    Available since
    Oct. 9, 2019
    Availability
    Academic Institutions and Nonprofits only
    131816-plasmid-map-sequence-id-259071

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  9. pBW011

    Plasmid
    #131814
    Purpose
    Vector with aTc inducible M. laminosus Fd1 mutant with the estrogen recepor-ligand binding domain fused between amino acids 35 and 36 with a C-terminal fused mRFP1
    Depositor
    Jonathan Silberg
    Article
    Wu et al bioRxiv May 23, 2019
    Insert
    M. laminosus Fd1 mutant with the estrogen recepor-ligand binding domain fused between amino acids 35 and 36
    Use
    Synthetic Biology
    Tags
    mRFP1
    Expression
    Mutation
    Promoter
    Available since
    Oct. 9, 2019
    Availability
    Academic Institutions and Nonprofits only
    131814-plasmid-map-sequence-id-259039

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  10. pBW009

    Plasmid
    #131811
    Purpose
    Vector with aTc inducible M. laminosus Fd1 mutant with the estrogen recepor-ligand binding domain fused between amino acids 30 and 31 with a C-terminal fused mRFP1
    Depositor
    Jonathan Silberg
    Article
    Wu et al bioRxiv May 23, 2019
    Insert
    M. laminosus Fd1 mutant with the estrogen recepor-ligand binding domain fused between amino acids 30 and 31
    Use
    Synthetic Biology
    Tags
    mRFP1
    Expression
    Mutation
    Promoter
    Available since
    Oct. 9, 2019
    Availability
    Academic Institutions and Nonprofits only
    131811-plasmid-map-sequence-id-259029

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  11. pBW007

    Plasmid
    #131808
    Purpose
    Vector with aTc inducible M. laminosus Fd1 mutant with the estrogen recepor-ligand binding domain fused between amino acids 25 and 26 with a C-terminal fused mRFP1
    Depositor
    Jonathan Silberg
    Article
    Wu et al bioRxiv May 23, 2019
    Insert
    M. laminosus Fd1 mutant with the estrogen recepor-ligand binding domain fused between amino acids 25 and 26
    Use
    Synthetic Biology
    Tags
    mRFP1
    Expression
    Mutation
    Promoter
    Available since
    Oct. 9, 2019
    Availability
    Academic Institutions and Nonprofits only
    131808-plasmid-map-sequence-id-259021

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  13. pMP11

    Plasmid
    #215559
    Purpose
    pKD46 with constitutively expressed Cas9 and an aTc gRNA targeting the ColE1 origin
    Depositor
    Brian Pfleger
    Article
    Mehrer et al Metab Eng. 2018 Jul;48:63-71. doi: 10.10
    Inserts
    Cas9
    gRNA targeting pBR322 origin of replication
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    J23105 and tet
    Available since
    May 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    215559-plasmid-map-sequence-id-434132

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  17. pFD115

    Plasmid
    #124768
    Purpose
    pFD115 carries cas9 controlled by an aTc-inducible Ptet promoter, a sgRNA controlled constitutively from PpflB S. aureus promoter to clone a guide between two BsaI sites and oriT on pLZ12 vector
    Depositor
    David Bikard
    Article
    Depardieu et al Methods. 2019 Aug 1. pii: S1046-2023(18)
    Inserts
    Ptet
    cas9
    term PpflB sgRNA (BsaI)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Ptet
    Available since
    Aug. 23, 2019
    Availability
    Academic Institutions and Nonprofits only
    124768-plasmid-map-sequence-id-246185

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Showing: 501 - 520 of 5682 results