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Plasmid#60395PurposeExpression of yeast beta tubulin (TUB2) - without Cterminal tail using GAL promoterDepositorInsertTUB2
UseTagsExpressionYeastMutationdeleted 429 - endPromoterGALAvailable sinceJan. 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRS426-TUB1-internal6xHis (1-440)
Plasmid#60391PurposeExpression of yeast alpha tubulin (TUB1) - internal 6xHis without Cterminal tail using a GAL promoterDepositorInsertTUB1 (TUB1 Budding Yeast)
UseTagsExpressionYeastMutationinternal 6xHis (see supplement figure 4), amino a…PromoterGALAvailable sinceJan. 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-200b-3p
Plasmid#103330PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-200b-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-200b-3p target (MIR200B Human)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCin4-3xFLAG-MKL1 S449A
Plasmid#19842DepositorInsertMKL1 S449A (MRTFA Human)
UseTags3xFLAGExpressionMammalianMutationchanged Serine 449 to AlaninePromoterAvailable sinceDec. 15, 2008AvailabilityAcademic Institutions and Nonprofits only -
pRS426-TUB1-internal6xHis - human TUBA1a Cterminal tail - delta Y
Plasmid#60393PurposeExpression of chimeric yeast alpha tubulin (TUB1) - internal 6xHis with human alpha tubulin Cterminal tail (TUBA1a) without Cterminal tyrosine (delta Y)DepositorInsertTUB1 (TUB1 Budding Yeast, Synthetic)
UseTagsExpressionYeastMutationinternal 6xHis (see supplement figure 4)PromoterGALAvailable sinceJan. 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
SRS-MTBD(22/19), AB(monomer), ux
Plasmid#22391DepositorInsertSeryl tRNA synthetase (SRS) – mouse cytoplasmic dynein microtubule binding domain (MTBD) fusion protein
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 24, 2009AvailabilityAcademic Institutions and Nonprofits only -
pFasBac+GFP-CAMSAP2 CC
Plasmid#59040Purposefor baculovirus expression of CAMSAP2 CC in Sf9 cellsDepositorInsertCAMSAP2 CC domain (CAMSAP2 Human)
UseTagsHis and eGFPExpressionInsectMutationPromoterAvailable sinceSept. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P1-human LIC1 G domain
Plasmid#74598Purposeused for expression in E. coli and purification. N-terminal GST tag and C-terminal Strep tag, aa 1-389DepositorInserthuman dynein light intermediate chain 1 amino acids 1-389 (DYNC1LI1 Human)
UseTagsN-terminal GST and Strep IIExpressionBacterialMutationPromoterT7Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
K349CLM-6His 215/347
Plasmid#24451DepositorInsertKinesin (KIF5B Human)
UseTagsHisExpressionBacterialMutationC7S/C65A/C168A/C174S/C294A/C330S, E215C, E347CPromoterAvailable sinceJuly 15, 2010AvailabilityAcademic Institutions and Nonprofits only -
pMT-GFP-Spindly
Plasmid#24282DepositorInsertSpindly (Spindly Fly)
UseTagsGFPExpressionInsectMutationPromoterAvailable sinceJune 1, 2010AvailabilityAcademic Institutions and Nonprofits only -
A337C/Y344C
Plasmid#24434DepositorInsertKinesin (KIF5B Human)
UseTags6XHis and GFPExpressionBacterialMutationC7S/C65A/C168A/C174S/C294A/C330S/C421A, A337C/Y…PromoterAvailable sinceJune 14, 2010AvailabilityAcademic Institutions and Nonprofits only -
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pET-21a-GFP-Patronin CC 1288–1457
Plasmid#59055Purposefor E. coli expression of Patronin CC truncationDepositorInsertPatronin (Patronin Fly)
UseTagsGFPExpressionBacterialMutationPromoterAvailable sinceAug. 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
pFasBac+GFP-CAMSAP3 CC
Plasmid#59042Purposefor baculovirus expression of mouse CAMSAP3 CC in Sf9 cellsDepositorInsertCAMSAP3 CC domain (Camsap3 Mouse)
UseTagsHis and eGFPExpressionInsectMutationPromoterAvailable sinceSept. 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET28a-Hook3 I136A, I139A, M142A
Plasmid#74617PurposeExpresses a triple mutant truncation of Hook3 in bacteria for purificationDepositorInserthuman Hook3 amino acids 1-239, I136A, I139A, M142A (HOOK3 Human)
UseTags6x His, Strep II, and superfolder GFPExpressionBacterialMutationD102A, E108APromoterT7Available sinceAug. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
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K222C/E334C
Plasmid#24435DepositorInsertKinesin (KIF5B Human)
UseTags6XHis and GFPExpressionBacterialMutationC7S/C65A/C168A/C174S/C294A/C330S/C421A, K222C/E…PromoterAvailable sinceJuly 13, 2010AvailabilityAcademic Institutions and Nonprofits only -
SRS-MTBD(26/19)ux, dimer, no myc
Plasmid#22382DepositorInsertSeryl tRNA synthetase (SRS) – mouse cytoplasmic dynein microtubule binding domain (MTBD) fusion protein
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceNov. 24, 2009AvailabilityAcademic Institutions and Nonprofits only -
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