We narrowed to 893 results for: V2

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AsCas12a

Plasmid

#188492

Purpose

Expresses FLAG tagged AsCas12a

Depositor

Isaac Hilton

Article

Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

Insert

Cas12a

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Sept. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pW211-lenti-spsgRNA-Esp3I-2kb-filler-pEF1s-NLS-mNeonGreen-P2A-BlastR

Plasmid

#170809

Purpose

Lentiviral vector to co-express an spsgRNA with NLS-mNeonGreen

Depositor

Kenneth Yamada

Article

Wang et al Cell. 2021 Jul 8;184(14):3702-3716.e30.

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Mutation

NA

Available Since

June 8, 2021

Availability

Academic Institutions and Nonprofits only

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pLP16_Lenti Scramble Control

Plasmid

#239417

Purpose

Negative control Lentiviral plasmid for SpCas9-based CRISPR KO

Depositor

Subhojit Roy

Article

Parra-Rivas et al STAR Protoc. 2025 Jul 21;6(3):103945. do

Insert

Scramble sequence

Use

Lentiviral

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-SOD2 MTS-3xHA-TALE array-Nt.BspD6I(C)-P2A-EGFP-UTR

Plasmid

#235204

Purpose

Plasmids for constructing mitoBEs v2

Depositor

Wensheng Wei

Article

Zhang et al Nature. 2025 Jan 22. doi: 10.1038/s41586

Insert

SOD2 MTS-3xHA-TALE array-Nt.BspD6I(C)-P2A-EGFP-UTR

Expression

Mammalian

Available Since

July 22, 2025

Availability

Academic Institutions and Nonprofits only

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LbdCas12a VPR

Plasmid

#188513

Purpose

Expresses FLAG tagged LbdCas12a VPR

Depositor

Isaac Hilton

Article

Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

Insert

dCas12a

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

D832A

Promoter

EF1a

Available Since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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LbdCas12a KRAB

Plasmid

#188503

Purpose

Expresses FLAG tagged LbdCas12a KRAB

Depositor

Isaac Hilton

Article

Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

Insert

dCas12a

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

D832A

Promoter

EF1a

Available Since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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LentiCRISPR-V2_mMSH2

Plasmid

#186156

Purpose

sgRNA targeting murine Msh2 gene

Depositor

David McFadden

Article

Povedano et al Cell Chem Biol. 2019 Sep 19;26(9):1315-1

Insert

Msh2_gRNA (Msh2 Mouse)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

July 6, 2022

Availability

Academic Institutions and Nonprofits only

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pLG1-puro Non-targeting sgRNA 3

Plasmid

#109003

Purpose

CRISPRi knockdown of targeted gene (to be used with Addgene #102244 or other dCas9-KRAB constructs)

Depositor

Jacob Corn

Article

Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

Insert

Non-targeting sgRNA #3

Use

Lentiviral

Expression

Mammalian

Promoter

mU6 promoter

Available Since

Aug. 22, 2018

Availability

Academic Institutions and Nonprofits only

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lenti-gRNA

Plasmid

#226867

Purpose

Lentiviral expression of Sp-gRNA with BlpI and BstXI restriction sites for gRNA spacer cloning. Also expresses BFP.

Depositor

Alexis Komor

Article

Osgood et al Methods Enzymol. 2025;712:351-404. doi:

Insert

Lenti Sp-gRNA

Use

Lentiviral

Promoter

mU6

Available Since

Feb. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pPD128 AND Gate, 3 repeats

Plasmid

#138937

Purpose

EYFP reporter vector for the Logic 37 AND 158 NOT 43 3 repeats promoter

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

Logic 37 AND 158 NOT 43 3 repeats

Use

Synthetic Biology

Expression

Mammalian

Promoter

Logic 37 AND 158 NOT 43 3 repeats

Available Since

Feb. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pET51b-NAD-cpLuc2

Plasmid

#135629

Purpose

Bacterial expression of a biosensor for measuring NAD+ levels at the point of care (V2)

Depositor

Kai Johnsson

Article

Yu et al Nat Metab. 2019 Dec;1(12):1219-1225. doi

Insert

NAD-cpLuc2 sensor

Tags

Hisx10 and Strep

Expression

Bacterial

Promoter

T7

Available Since

March 4, 2020

Availability

Academic Institutions and Nonprofits only

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Cas12j2

Plasmid

#188498

Purpose

Expresses FLAG tagged Cas12j2

Depositor

Isaac Hilton

Article

Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

Insert

Cas12j2

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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LbCas12a

Plasmid

#188493

Purpose

Expresses FLAG tagged LbCas12a

Depositor

Isaac Hilton

Article

Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

Insert

Cas12a

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Sept. 28, 2022

Availability

Academic Institutions and Nonprofits only

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EFS eGFP hPGK BSD

Plasmid

#188520

Purpose

Backbone for CRISPR interference (CRISPRi) isogenic PAM testing

Depositor

Isaac Hilton

Article

Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

Type

Empty backbone

Use

Lentiviral

Tags

eGFP

Expression

Mammalian

Available Since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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V1-MESA-45F-M-dCas9

Plasmid

#84504

Purpose

MESA target chain with V1-MESA ectodomain, 45 extracellular linkers, a flag tag, M cleavage sequence, and dCas9-VP64

Depositor

Joshua Leonard

Article

Schwarz et al Nat Chem Biol. 2016 Dec 12. doi: 10.1038

Insert

V2-MESA-35F-M-dCas9

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

April 12, 2017

Availability

Academic Institutions and Nonprofits only

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lenti SYN-SVI-dCas9-VPR

Plasmid

#164575

Purpose

Expresses an intron-containing dCas9-VPR fusion driven by human SYN promoter

Depositor

Jeremy Day

Article

Carullo et al eNeuro. 2021 Jul 26. pii: ENEURO.0188-21

Insert

FLAG-dCas9-VPR containing an SV40 intron

Use

CRISPR and Lentiviral

Tags

FLAG

Expression

Mammalian

Available Since

Feb. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pW213-lenti-sasgRNA-Esp3I-2kb-filler-pEF1s-NLS-mNeonGreen-P2A-BlastR

Plasmid

#170811

Purpose

Lentiviral vector to co-express an sasgRNA with NLS-mNeonGreen

Depositor

Kenneth Yamada

Article

Wang et al Cell. 2021 Jul 8;184(14):3702-3716.e30.

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Mutation

NA

Available Since

June 8, 2021

Availability

Academic Institutions and Nonprofits only

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MIGR1-U6gRNA1-Filler-v1

Plasmid

#237399

Purpose

For inserting a single guide RNA or later cloning two U6-gRNA cassettes with MIGR1-U6gRNA1-Filler-v2.

Depositor

Zuoming Sun

Article

Zhong et al STAR Protoc. 2025 Jun 20;6(2):103831. do

Type

Empty backbone

Use

CRISPR and Retroviral

Expression

Mammalian

Promoter

U6, PGK

Available Since

June 27, 2025

Availability

Academic Institutions and Nonprofits only

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XZ031 CMV-TO-CCR6-ADAR2dd(E488Q, T501A)

Plasmid

#233393

Purpose

CCR6-ADAR2dd(E488Q, T501A) with a short 4 amino acid linker and no V2 tail

Depositor

Xiaojing Gao

Article

Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

Insert

CCR6-ADAR2dd

Expression

Mammalian

Mutation

E488Q, T501A on ADAR2dd

Promoter

CMV

Available Since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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LSM044-3 CMV-TO-CCR7-ADAR2dd(E488Q, T501A) (FLP-IN)

Plasmid

#233395

Purpose

CCR7-ADAR2dd(E488Q, T501A) with a short 4 amino acid linker and no V2 tail

Depositor

Xiaojing Gao

Article

Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

Insert

CCR7-ADAR2dd

Expression

Mammalian

Mutation

E488Q, T501A on ADAR2dd

Promoter

CMV

Available Since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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