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pLU-CMV-Flag-hSesn2 L128C/H113E

Plasmid

#111778

Purpose

Mammalian expression or 3rd generation lentivirus generation of hSesn2

Depositor

Jun Hee Lee

Article

Kim et al Nat Commun. 2015 Nov 27;6:10025. doi: 10

Insert

hSesn2 (SESN2 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

L128C/H113E

Promoter

Available since

June 13, 2018

Availability

Academic Institutions and Nonprofits only

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pLU-CMV-Flag-hSesn2 H132A

Plasmid

#111780

Purpose

Mammalian expression or 3rd generation lentivirus generation of hSesn2

Depositor

Jun Hee Lee

Article

Kim et al Nat Commun. 2015 Nov 27;6:10025. doi: 10

Insert

hSesn2 (SESN2 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

H132A

Promoter

Available since

June 13, 2018

Availability

Academic Institutions and Nonprofits only

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pLU-CMV-Flag-hSesn2 D406A

Plasmid

#111794

Purpose

Mammalian expression or 3rd generation lentivirus generation of hSesn2

Depositor

Jun Hee Lee

Article

Kim et al Nat Commun. 2015 Nov 27;6:10025. doi: 10

Insert

hSesn2 (SESN2 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

D406A

Promoter

Available since

June 13, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pLU-CMV-Flag-hSesn2 Y127F

Plasmid

#111781

Purpose

Mammalian expression or 3rd generation lentivirus generation of hSesn2

Depositor

Jun Hee Lee

Article

Kim et al Nat Commun. 2015 Nov 27;6:10025. doi: 10

Insert

hSesn2 (SESN2 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

Y127F

Promoter

Available since

June 13, 2018

Availability

Academic Institutions and Nonprofits only

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pLU-CMV-Flag-hSesn2 C125S

Plasmid

#111777

Purpose

Mammalian expression or 3rd generation lentivirus generation of hSesn2

Depositor

Jun Hee Lee

Article

Kim et al Nat Commun. 2015 Nov 27;6:10025. doi: 10

Insert

hSesn2 (SESN2 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

C125S

Promoter

Available since

June 13, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pLU-CMV-Flag-hSesn2 P87S

Plasmid

#111779

Purpose

Mammalian expression or 3rd generation lentivirus generation of hSesn2

Depositor

Jun Hee Lee

Article

Kim et al Nat Commun. 2015 Nov 27;6:10025. doi: 10

Insert

hSesn2 (SESN2 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

P87S

Promoter

Available since

June 13, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pLU-CMV-Flag-hSesn2 C430S

Plasmid

#111786

Purpose

Mammalian expression or 3rd generation lentivirus generation of hSesn2

Depositor

Jun Hee Lee

Article

Kim et al Nat Commun. 2015 Nov 27;6:10025. doi: 10

Insert

hSesn2 (SESN2 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

C430S

Promoter

Available since

June 13, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pLU-CMV-Flag-hSesn2 C399S

Plasmid

#111783

Purpose

Mammalian expression or 3rd generation lentivirus generation of hSesn2

Depositor

Jun Hee Lee

Article

Kim et al Nat Commun. 2015 Nov 27;6:10025. doi: 10

Insert

hSesn2 (SESN2 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

C399S

Promoter

Available since

June 13, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pLU-CMV-Flag-hSesn2 T336A/F337A

Plasmid

#111787

Purpose

Mammalian expression or 3rd generation lentivirus generation of hSesn2

Depositor

Jun Hee Lee

Article

Kim et al Nat Commun. 2015 Nov 27;6:10025. doi: 10

Insert

hSesn2 (SESN2 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

T336A/F337A

Promoter

Available since

June 13, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pLU-CMV-Flag-hSesn2 Q340A/D341A

Plasmid

#111788

Purpose

Mammalian expression or 3rd generation lentivirus generation of hSesn2

Depositor

Jun Hee Lee

Article

Kim et al Nat Commun. 2015 Nov 27;6:10025. doi: 10

Insert

hSesn2 (SESN2 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

Q340A/D341A

Promoter

Available since

June 13, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pLU-CMV-Flag-hSesn2 C204S

Plasmid

#111782

Purpose

Mammalian expression or 3rd generation lentivirus generation of hSesn2

Depositor

Jun Hee Lee

Article

Kim et al Nat Commun. 2015 Nov 27;6:10025. doi: 10

Insert

hSesn2 (SESN2 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

C204S

Promoter

Available since

June 7, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pBW106

Plasmid

#111138

Purpose

For the expression of rat AANAT with Met-Ser-Ile N-terminus in S. cerevisiae

Depositor

Alexander Varshavsky

Article

Wadas et al J Biol Chem. 2016 Aug 12;291(33):17178-9

Insert

MSI-ratAANAT-3FLAG (Leu2 deletion) (Aanat Rat)

Use

Tags

3x Flag

Expression

Yeast

Mutation

Leu2 deletion

Promoter

Gal1

Available since

June 4, 2018

Availability

Academic Institutions and Nonprofits only

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pET28a-MED11

Plasmid

#15414

Depositor

Joan Conaway, Ronald Conaway

Article

Sato et al J Biol Chem. 2003 Dec 12. 278(50):49671-

Insert

MED11 (Med11 Mouse)

Use

Tags

His

Expression

Bacterial

Mutation

N/A

Promoter

Available since

Aug. 10, 2007

Availability

Academic Institutions and Nonprofits only
PGK mScarlet-LC3B

Plasmid

#200083

Purpose

Mammalian expression of fluorescently-tagged marker for autophagic vesicles

Depositor

Erika Holzbaur

Article

Dou et al Cell Rep. 2023 May 1;42(5):112448. doi:

Insert

Map1lc3b (Map1lc3b Rat)

Use

Tags

mScarlet

Expression

Mammalian

Mutation

Promoter

PGK

Available since

May 22, 2023

Availability

Academic Institutions and Nonprofits only

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mCherry-Sec23A

Plasmid

#166894

Purpose

mammalian expression of Sec23A tagged with mCherry

Depositor

Jennifer Lippincott-Schwartz

Article

Weigel et al Cell. 2021 Apr 29;184(9):2412-2429.e16.

Insert

SEC23A (SEC23A Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 14, 2021

Availability

Academic Institutions and Nonprofits only

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mEmerald-Sec23A

Plasmid

#166893

Purpose

mammalian expression of Sec23A tagged with mEmerald

Depositor

Jennifer Lippincott-Schwartz

Article

Weigel et al Cell. 2021 Apr 29;184(9):2412-2429.e16.

Insert

SEC23A (SEC23A Human)

Use

Tags

mEmerald

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

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AAV-CAG-SPOTlight-U2GCR

Plasmid

#164819

Purpose

Reporter to measure ISR state-dependent protein synthesis

Depositor

Nicole Calakos

Article

Helseth et al Science. 2021 Apr 23;372(6540). pii: 372

Insert

SPOTlight-U2GCR

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

June 7, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV-IRES-Renilla Luciferase-IRES-Gateway-Firefly Luciferase (pIRIGF)

Plasmid

#101139

Purpose

Luciferase reporter plasmid. Note that both renilla luciferase and ORF-firefly luciferase are encoded by a single mRNA and both are translated independently via internal ribosomal binding sites.

Depositor

William Kaelin

Article

Lu et al Science. 2014 Jan 17;343(6168):305-9. do

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 11, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-EWB-DIO-myriRFP670V5-P2A-post-eGRASP

Plasmid

#111585

Purpose

An AAV vector that expresses double floxed myristoylated iRFP670 with V5 tag and post-eGRASP linked by self-cleaving P2A peptide under the Ef1a promoter.

Depositor

Bong-Kiun Kaang

Article

Choi et al Science. 2018 Apr 27;360(6387):430-435.

Insert

myriRFP670V5-P2A-post-eGRASP

Use

AAV

Tags

Expression

Mutation

Promoter

Ef1a

Available since

June 12, 2018

Availability

Academic Institutions and Nonprofits only

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pET41-MBP-t-A3A-t-His

Plasmid

#109231

Purpose

Bacterial expression of A3A with N-terminal MBP an C-terminal His tag, both cleavable with TEV protease

Depositor

Rahul Kohli

Article

Schutsky et al Nat Biotechnol. 2018 Oct 8. pii: nbt.420

Insert

human APOBEC3A (APOBEC3A Optimized for E. coli expression, Human)

Use

Tags

His and MBP

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 16, 2018

Availability

Academic Institutions and Nonprofits only

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