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pFA6a-6xGLY-VSV-G-hphMX4

Plasmid

#20773

Depositor

Mark Hochstrasser

Article

Funakoshi et al Yeast. 2009 Feb 25. 26(3):185-192.

Type

Empty backbone

Use

Pcr-based c-terminal tagging

Expression

Yeast

Available Since

March 23, 2009

Availability

Academic Institutions and Nonprofits only

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pCR8/GW_TOPO_Cdk12_Untagged

Plasmid

#127176

Purpose

Untagged mouse Cdk12 transgene (NM_001109626.1 isoform) cloned into Gateway entry vector

Depositor

Phil Sharp

Article

Dubbury et al Nature. 2018 Dec;564(7734):141-145. doi:

Insert

Mouse Cyclin Dependent Kinase 12 (Cdk12 Mouse)

Use

Entry vector with transgene for gateway cloning

Tags

None

Promoter

None

Available Since

July 5, 2019

Availability

Academic Institutions and Nonprofits only

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PCRII apoEb

Plasmid

#121901

Purpose

Plasmid for making zebrafish apoEb in situ probe

Depositor

Steven Farber

Article

Otis et al Dis Model Mech. 2015 Mar;8(3):295-309. d

Insert

Apoeb (apoeb Zebrafish)

Use

Unspecified

Available Since

May 10, 2019

Availability

Academic Institutions and Nonprofits only

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pCR8 PALI1

Plasmid

#114442

Purpose

Entry vector for gateway cloning containing human PALI1 coding sequence

Depositor

Adrian Bracken

Article

Conway et al Mol Cell. 2018 May 3;70(3):408-421.e8. d

Insert

PALI1 (LCOR Human)

Use

Gateway entry cloning

Promoter

None

Available Since

Sept. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pCR8-DJ1-WT

Plasmid

#29354

Depositor

Mark Cookson

Article

Blackinton et al Brain Res Mol Brain Res. 2005 Mar 24. 13

Insert

DJ1 (PARK7 Human)

Use

Gateway Cloning

Available Since

March 31, 2011

Availability

Academic Institutions and Nonprofits only
pCRII Pig5

Plasmid

#16499

Depositor

Bert Vogelstein

Article

Polyak et al Nature. 1997 Sep 18. 389(6648):300-5.

Insert

Pig5 (ANO9 Human)

Expression

Bacterial

Mutation

Partial cDNA clone

Available Since

April 24, 2008

Availability

Academic Institutions and Nonprofits only
pcR295/527

Plasmid

#17679

Depositor

David Shalloway

Article

Bagrodia et al Nature. 1991 Jan 10. 349(6305):172-5.

Insert

c-src K295R Y527F (SRC Chicken)

Expression

Mammalian

Mutation

Lys 295 changed to Arg. Tyr 527 changed to Phe.

Available Since

April 15, 2008

Availability

Academic Institutions and Nonprofits only
pCRII-Topo Vglut2 in situ probe

Plasmid

#45639

Depositor

Jeffrey Macklis

Article

Azim et al Nat Neurosci. 2009 Oct;12(10):1238-47. d

Insert

Vglut2 in situ probe (Slc17a6 Mouse)

Use

In situ

Mutation

fragment contains nt 2477-2993 of slc17a6 (Vglut2…

Available Since

June 3, 2013

Availability

Academic Institutions and Nonprofits only
pFA6a-kanMX6-PGAL1-3HA

Plasmid

#41608

Depositor

John Pringle

Article

Longtine et al Yeast. 1998 Jul;14(10):953-61.

Insert

GAL1 promoter (GAL1 Budding Yeast)

Use

Yeast genomic targeting

Tags

3xHA, A. gossypii translation elongation factor 1…

Available Since

Dec. 10, 2012

Availability

Academic Institutions and Nonprofits only

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PCRbluntII-pHES7-STOPgRNA

Plasmid

#204349

Purpose

gRNA to target pig HES7 stop codon

Depositor

Li-Fang Chu

Article

Conrad et al Stem Cell Reports. 2023 Dec 12;18(12):23

Insert

Pig HES7-STOP-gRNA (HES7 Sus domesticus (pig))

Expression

Mammalian

Available Since

Jan. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pCrepusculo

Plasmid

#190156

Purpose

Regulates bacterial expression of target gene in blue-light-repressed manner. Based on Nakamurella multipartita PAL.

Depositor

Andreas Moeglich

Article

Ranzani et al ACS Synth Biol. 2022 Oct 21;11(10):3482-

Type

Empty backbone

Tags

His6 tag

Expression

Bacterial

Available Since

Nov. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pcryBFP_Umyo18bGFP

Plasmid

#172527

Purpose

alphaA-crystallin promoter (cry) drives GFP expression in the lens. The 600bp unc-45b muscle-specific promoter drives expression of GFP-tagged zebrafish Myo18b.

Depositor

Joachim Berger

Article

Berger et al Hum Mol Genet. 2017 Mar 15;26(6):1146-11

Insert

myo18b

Expression

Mammalian

Available Since

Sept. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pCR2.1_HBB_N

Plasmid

#87847

Purpose

pCR2.1-based plasmid holding a 309-bp fragment containing the exon-1-exon-2 splice border of normal human β-globin mRNA

Depositor

Marina Kleanthous

Article

Patsali et al Int J Mol Sci. 2020 Sep 11;21(18). pii:

Insert

Homo sapiens hemoglobin subunit beta (HBB), Normal cDNA fragment (Exon1/2) (HBB Human)

Expression

Bacterial

Available Since

Oct. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pCRISP_IS

Plasmid

#120425

Purpose

This plasmid encodes the complete CRISPR/dCas9 machinery for repressing transposition of the following bacterial Insertion Sequences: IS1, IS3 and IS5.

Depositor

Tamás Fehér

Article

Nyerges et al Synth Biol (Oxf). 2019;4(1):ysz008. doi:

Insert

CRISPR spacers targeting IS1, IS5, IS3

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Promoter

constitutive

Available Since

Jan. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pCR1276

Plasmid

#111115

Purpose

Expresses RAD51C_+_56769980.23-P1P2 sgRNA in pCR1068

Depositor

Jacob Corn

Article

Richardson et al Nat Genet. 2018 Aug;50(8):1132-1139. doi

Insert

RAD51C gRNA (RAD51C Human)

Use

Lentiviral

Available Since

Oct. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pCR1162

Plasmid

#111098

Purpose

Expresses FANCA_-_89883048.23-P1P2 sgRNA in pCR1068

Depositor

Jacob Corn

Article

Richardson et al Nat Genet. 2018 Aug;50(8):1132-1139. doi

Insert

FANCA gRNA (FANCA Human)

Use

Lentiviral

Available Since

Oct. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pCRISPaint-HA

Plasmid

#67171

Purpose

CRISPaint universal donor for HA fusion tagging

Depositor

Veit Hornung

Article

Schmid-Burgk et al Nat Commun. 2016 Jul 28;7:12338. doi: 10

Insert

HA

Available Since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pCri-8a

Plasmid

#61317

Purpose

Protein expression.

Depositor

F Xavier Gomis-Ruth

Article

Goulas et al PLoS One. 2014 Nov 11;9(11):e112643. doi

Insert

Green fluorescent protein

Tags

His-tag

Expression

Bacterial

Available Since

April 1, 2015

Availability

Academic Institutions and Nonprofits only
pCri-7b

Plasmid

#61330

Purpose

Protein expression.

Depositor

F Xavier Gomis-Ruth

Article

Goulas et al PLoS One. 2014 Nov 11;9(11):e112643. doi

Insert

Green fluorescent protein

Tags

His-tag

Expression

Bacterial

Available Since

Jan. 9, 2015

Availability

Academic Institutions and Nonprofits only
pCri-4b

Plasmid

#61328

Purpose

Protein expression.

Depositor

F Xavier Gomis-Ruth

Article

Goulas et al PLoS One. 2014 Nov 11;9(11):e112643. doi

Insert

Green fluorescent protein

Tags

His-tag and TRX-tag

Expression

Bacterial

Available Since

Jan. 9, 2015

Availability

Academic Institutions and Nonprofits only