We narrowed to 4,553 results for: pcr

1. NTPCR gRNA (BRDN0001147742)

   Plasmid

   #76877

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human NTPCR

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   NTPCR (NTPCR Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCR2.1-Ins2-842

   Plasmid

   #53969

   Purpose

   842 bp insert from mouse Ins2 gene used as a control for methylation-specific PCR assays

   Depositor

   Kevin Ferreri

   Article

   Husseiny et al PLoS One. 2012;7(10):e47942. doi: 10.137

   Insert

   mouse Ins2 gene partial (Ins2 Mouse)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   lac

   Available Since

   July 25, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pPCR-Script-mLIP

   Plasmid

   #49194

   Purpose

   initial cloning vector for mLIP fragment of C/EBPbeta

   Depositor

   Jed Friedman

   Insert

   C/EBPbeta (LIP fragment) (Cebpb Mouse)
   

   Use

   Cloning vector

   Tags

   Expression

   Bacterial

   Mutation

   only the LIP fragment

   Promoter

   lac

   Available Since

   Nov. 14, 2013

   Availability

   Academic Institutions and Nonprofits only

4. pCRII-Topo Frmd4b

   Plasmid

   #45867

   Depositor

   Jeffrey Macklis

   Article

   Molyneaux et al J Neurosci. 2009 Sep 30;29(39):12343-54.

   Insert

   Frmd4b in situ probe (Frmd4b Mouse)
   

   Use

   In situ

   Tags

   Expression

   Mutation

   contains bp# 3933-4535 of NM_145148.2

   Promoter

   Available Since

   July 9, 2013

   Availability

   Academic Institutions and Nonprofits only

5. pCRII-Topo Grp

   Plasmid

   #45871

   Depositor

   Jeffrey Macklis

   Insert

   GRP in situ probe (Grp Mouse)
   

   Use

   In situ

   Tags

   Expression

   Mutation

   nt 66-504 on NM_175012

   Promoter

   Available Since

   June 11, 2013

   Availability

   Academic Institutions and Nonprofits only

6. pCRII-Topo Tmtc4

   Plasmid

   #45869

   Depositor

   Jeffrey Macklis

   Article

   Molyneaux et al J Neurosci. 2009 Sep 30;29(39):12343-54.

   Insert

   TMTC4 in situ probe (Tmtc4 Mouse)
   

   Use

   In situ

   Tags

   Expression

   Mutation

   nt 1220-1798 on BC031368

   Promoter

   Available Since

   June 11, 2013

   Availability

   Academic Institutions and Nonprofits only

7. pCR8-DJ1-E64D

   Plasmid

   #29356

   Depositor

   Mark Cookson

   Article

   Blackinton et al Brain Res Mol Brain Res. 2005 Mar 24. 13

   Insert

   DJ1 (PARK7 Human)
   

   Use

   Entry vector

   Tags

   Expression

   Mutation

   Glu64Asp

   Promoter

   Available Since

   March 31, 2011

   Availability

   Academic Institutions and Nonprofits only

8. 27 pCR plcg 1 CDS

   Plasmid

   #13058

   Depositor

   Nathan Lawson

   Article

   Lawson et al Genes Dev. 2003 Jun 1. 17(11):1346-51.

   Insert

   plcg1 (plcg1 Zebrafish)
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   June 18, 2010

   Availability

   Academic Institutions and Nonprofits only

9. pSL3617 (pCRISPR(BbsI)_Pse)

   Plasmid

   #200902

   Purpose

   Expresses Pseudoalteromonas CRISPR RNA from a U6 promoter. The CRISPR array contains two BbsI sites for spacer cloning.

   Depositor

   Samuel H. Sternberg

   Article

   Lampe et al Nat Biotechnol. 2023 Mar 29. doi: 10.103

   Insert

   PseCRISPR (entry)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   May 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCMV-tadDE-PcrA M6-UGI

    Plasmid

    #221236

    Purpose

    Express HACE Editor as a fusion of the engineered dual base editor TadE that can perform citosine and adenine deamination and PcrA helicase.

    Depositor

    Fei Chen

    Article

    Chen et al Science. 2024 Oct 11;386(6718):eadn5876.

    Insert

    tadDE-PcrA M6-UGI
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCROPseq-VEX-optimized-scaffold

    Plasmid

    #203321

    Purpose

    Modified CROPseq vector for efficient CRISPR screens in hematopoiesis

    Depositor

    Vijay Sankaran

    Article

    Martin-Rufino et al Cell. 2023 Apr 27:S0092-8674(23)00332-X.

    Insert

    sgRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. PCR4-Shh::lacZ-H11

    Plasmid

    #139098

    Purpose

    enSERT LacZ reporter vector for site-specific integration into the H11 locus (contains Shh minimal promoter)

    Depositor

    Len Pennacchio

    Article

    Kvon et al Cell. 2020 Mar 6. pii: S0092-8674(20)302

    Type

    Empty backbone

    Use

    CRISPR and Mouse Targeting

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Shh

    Available Since

    March 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCR2.1 Clover-LMNA Donor

    Plasmid

    #122508

    Purpose

    Homology repair template for in frame (first exon) clover knock-in of human LMNA gene

    Depositor

    Graham Dellaire

    Article

    Pinder et al Nucleic Acids Res. 2015 Oct 30;43(19):93

    Insert

    Homology Repair Template for Human LMNA (N-Terminal Clover Tag) (LMNA Human)
    

    Use

    Homology repair template plasmid (donor plasmid)

    Tags

    Clover

    Expression

    Mutation

    Promoter

    Available Since

    April 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. PCR4-Hsp68::lacZ-H11

    Plasmid

    #139099

    Purpose

    enSERT LacZ reporter vector for site-specific integration into the H11 locus (contains Hsp68 minimal promoter)

    Depositor

    Len Pennacchio

    Article

    Kvon et al Cell. 2020 Mar 6. pii: S0092-8674(20)302

    Type

    Empty backbone

    Use

    CRISPR and Mouse Targeting

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Hsp68

    Available Since

    March 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCRISPEY-GAL-Kan-ADE2

    Plasmid

    #232105

    Purpose

    Expresses galactose-inducible CRISPR guide RNA and repair template for creating a frameshift mutation in ADE2 gene of yeast.

    Depositor

    Jeffrey Lewis

    Article

    Stuecker et al G3 (Bethesda). 2025 Aug 4:jkaf175. doi:

    Insert

    ADE2 gRNA and repair template
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Repair template introduces C at nt 466 of ADE2 to…

    Promoter

    GAL7

    Available Since

    Feb. 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCRISPaint-mScarlet-PuroR [M1G]

    Plasmid

    #171809

    Purpose

    Universal donor plasmid for CRISPitope method encoding mScarlet fluorescent protein and puromycin resistance cassette [p.M1G]

    Depositor

    Michael Hoelzel

    Article

    Effern et al Immunity. 2020 Sep 15;53(3):564-580.e9.

    Insert

    mScarlet-T2A-PuroR
    

    Use

    Gene tagging

    Tags

    Expression

    Mutation

    PuroR: Changed Methionin 1 to Glycine.

    Promoter

    Available Since

    July 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCRISPR-HOT-Clover-BlastR

    Plasmid

    #138569

    Purpose

    Universal Cleavable Donor Plasmid for tagging with Clover-BlastR using NHEJ

    Depositor

    Hans Clevers

    Article

    Artegiani et al Nat Cell Biol. 2020 Mar;22(3):321-331. d

    Insert

    Clover-BlastR
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    March 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCR-U6-gRNA-TRE3G-TdTomato

    Plasmid

    #192658

    Purpose

    A plasmid encoding TRE3G sgRNA and TRE3G-promoter-TdTomato

    Depositor

    Jeffrey Miner

    Article

    Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

    Insert

    TdTomato
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6, TRE3G

    Available Since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCR-U6-gRNA-miniCMV-TdTomato

    Plasmid

    #192656

    Purpose

    A plasmid encoding miniCMV sgRNA and miniCMV-promoter-TdTomato

    Depositor

    Jeffrey Miner

    Article

    Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

    Insert

    TdTomato
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6, miniCMV

    Available Since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCR-blunt-TOPO-FNDC5

    Plasmid

    #35970

    Depositor

    Bruce Spiegelman

    Article

    Bostrom et al Nature. 2012 Jan 11;481(7382):463-8. doi

    Insert

    fibronectin type III domain containing 5 (Fndc5 Mouse)
    

    Use

    Cloning vector

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    April 2, 2012

    Availability

    Academic Institutions and Nonprofits only