We narrowed to 10,315 results for: plasmids 101

1. pKP2032

   Plasmid

   #167651

   Purpose

   Express His6-ExbB S43A, S45A, S67A, S80A, S82A, S84A, S98A, S101A, T111A, S112A, S221A, S229A

   Depositor

   Kathleen Postle

   Insert

   his6-exbB S43A, S45A, S67A, S80A, S82A, S84A, S98A, S101A, T111A, S112A, S221A, S229A
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   ExbB S43A, S45A, S67A, S80A, S82A, S84A, S98A, S1…

   Promoter

   Available Since

   July 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCLX-PGK-hCDK4

   Plasmid

   #114314

   Purpose

   Constitutive expression of the CDK4 protein

   Depositor

   Patrick Salmon

   Insert

   CDK4 (CDK4 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Sept. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET21b-Casp1-His

   Plasmid

   #11809

   Depositor

   Guy Salvesen

   Insert

   Caspase-1 (CASP1 Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   May 12, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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4. pnEA-NpM-HsTOB1_Q

   Plasmid

   #147315

   Purpose

   Bacterial Expression of HsTOB1

   Depositor

   Elisa Izaurralde

   Insert

   HsTOB1 (TOB1 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   one non silent mutation F309L compared to the seq…

   Promoter

   Available Since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pnEA-NpM-HsTOB1_1-138_Q

   Plasmid

   #147316

   Purpose

   Bacterial Expression of HsTOB1_1-138

   Depositor

   Elisa Izaurralde

   Insert

   HsTOB1_1-138 (TOB1 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   one silent mutation compared to teh sequence give…

   Promoter

   Available Since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLNHA-C1-HsTOB1_Q

   Plasmid

   #147286

   Purpose

   Mammalian Expression of HsTOB1

   Depositor

   Elisa Izaurralde

   Insert

   HsTOB1 (TOB1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   one silent and one non silent S228P mutation comp…

   Promoter

   Available Since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCC324

   Plasmid

   #110165

   Purpose

   miniMos vector expresses ERKKTR-mClover and mCherry-H2B in C. elegans VPCs; request with plasmids pCC335 and pCC336 for unphosphorylated and phosphomimetic controls (Contains Neomycin resistance)

   Depositor

   Iva Greenwald

   Article

   de la Cova et al Dev Cell. 2017 Sep 11;42(5):542-553.e4.

   Insert

   ERK-KTR-mClover-T2A-mCherry-H2B
   

   Use

   Minimos transposon

   Tags

   mCherry and mClover

   Expression

   Worm

   Mutation

   R61K change made in ERK-KTR CDS

   Promoter

   lin-31 promoter/enhancer

   Available Since

   Oct. 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. INT_GFPsp

   Plasmid

   #212144

   Purpose

   Preparatory plasmid for operon integration into the genome using CRISPR-Cas12a. Plasmid can be removed by incubating cells at 37 C.

   Depositor

   Kang Zhou

   Insert

   VchTniQ, VchCas8, VchCas7, VchCas6, VchTnsA, VchTnsB, VchTnsC, green fluorescent protein
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pJ23119

   Available Since

   Jan. 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. INT_GFPsp_CamRcargo

   Plasmid

   #212143

   Purpose

   Preparatory plasmid for gene disruption into the genome using CRISPR-Cas12a. Plasmid can be removed by incubating cells at 37 C.

   Depositor

   Kang Zhou

   Insert

   VchTniQ, VchCas8, VchCas7, VchCas6, VchTnsA, VchTnsB, VchTnsC, green fluorescent protein
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pJ23119

   Available Since

   Jan. 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pRDAX-554 N-Tag GFP[1] L10 Stat3 EBPF2

    Plasmid

    #130101

    Purpose

    PPI (BiFC/TriFC) assay vector

    Depositor

    Gary Lewin

    Insert

    Stat3 (Stat3 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pRW402-P1int-BXB1

    Plasmid

    #235979

    Purpose

    Plasmid used for constructing donor DNA for BXB1-compatible p1 integration. You can insert your GOIs into this plasmid by using Golden Gate Assembly.

    Depositor

    Chang Liu

    Article

    Pisera et al bioRxiv [Preprint]. 2024 Nov 30:2024.11.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    April 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSCM167.2-Venus-VF-hADAM10(aa1-213)

    Plasmid

    #189554

    Purpose

    Protein expression ADAM10 in mammalian cells. Contains only prodomain--amino acids 1-213

    Depositor

    Haian Fu

    Insert

    truncated ADAM metallopeptidase domain 10 (ADAM10 Human)
    

    Use

    Tags

    VF

    Expression

    Mammalian

    Mutation

    contains aa 1-213

    Promoter

    Available Since

    Feb. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMBA324

    Plasmid

    #214741

    Purpose

    Low copy glmS-nadE plasmid containing kanamycin as antibiotic resistance gene (ARG) for validation

    Depositor

    Tae Seok Moon

    Article

    Amrofell et al Cell Rep Methods. 2023 Dec 18;3(12):1006

    Insert

    BBa_J23108-RBS(8455)-glmS-RBS(5392)-nadE
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSV015

    Plasmid

    #214738

    Purpose

    Low copy thyA-infA plasmid containing kanamycin as antibiotic resistance gene (ARG) for validation

    Depositor

    Tae Seok Moon

    Article

    Amrofell et al Cell Rep Methods. 2023 Dec 18;3(12):1006

    Insert

    BBa_J23108-RBS(7504)-thyA-RBS(4427)-infA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. INT_Cargo_PgyrA_katG_dadA6

    Plasmid

    #212142

    Purpose

    Cargo plasmid for integrating PgyrA expression cassette of catalase-peroxidase and D-amino acid dehydrogenase into the genome. Plasmid can be removed by incubating cells at 37 C.

    Depositor

    Kang Zhou

    Insert

    catalase-peroxidase, D-amino acid dehydrogenase (katG katG: E. coli, dadA6: Cupriavidus necator)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PgyrA

    Available Since

    Jan. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGEX-MK1

    Plasmid

    #29638

    Depositor

    Bruce Tempel

    Insert

    Mk1 (Kcna1 Mouse)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    May 5, 2011

    Availability

    Academic Institutions and Nonprofits only

17. pSCM167-Venus-VF-hADAM10(aa220-654)

    Plasmid

    #189560

    Purpose

    Protein expression ADAM10 in mammalian cells. Contains metalloprotease, disintegrin, and cysteine rich domains--amino acids 220-654

    Depositor

    Haian Fu

    Insert

    truncated ADAM metallopeptidase domain 10 (ADAM10 Human)
    

    Use

    Tags

    VF

    Expression

    Mammalian

    Mutation

    contains aa 220-654

    Promoter

    Available Since

    Feb. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSCM167-Venus-VF-hADAM10(aa1-459)

    Plasmid

    #189559

    Purpose

    Protein expression ADAM10 in mammalian cells. Contains prodomain and metalloprotease domain--amino acids 1-459

    Depositor

    Haian Fu

    Insert

    truncated ADAM metallopeptidase domain 10 (ADAM10 Human)
    

    Use

    Tags

    VF

    Expression

    Mammalian

    Mutation

    contains aa 1-459

    Promoter

    Available Since

    Nov. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pSCM167.2-Venus-VF-hADAM10(aa455-550)

    Plasmid

    #189556

    Purpose

    Protein expression ADAM10 in mammalian cells. Contains only disintegrin domain--amino acids 455-550

    Depositor

    Haian Fu

    Insert

    truncated ADAM metallopeptidase domain 10 (ADAM10 Human)
    

    Use

    Tags

    VF

    Expression

    Mammalian

    Mutation

    contains aa 455-550

    Promoter

    Available Since

    Feb. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pSCM167-Venus-VF-hADAM10(aa220-459)

    Plasmid

    #189555

    Purpose

    Protein expression ADAM10 in mammalian cells. Contains only metalloprotease domain--amino acids220-459

    Depositor

    Haian Fu

    Insert

    truncated ADAM metallopeptidase domain 10 (ADAM10 Human)
    

    Use

    Tags

    VF

    Expression

    Mammalian

    Mutation

    contains aa 220-459

    Promoter

    Available Since

    Nov. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge