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pAc5.1B-EGFP-DmTral_394-543-V5His6_D

Plasmid

#146134

Purpose

Insect Expression of DmTral_394-543

Depositor

Elisa Izaurralde

Article

Tritschler et al Mol Cell Biol. 2008 Nov . 28(21):6695-70

Insert

DmTral_394-543 (tral Fly)

Expression

Insect

Mutation

two silent mutations (nucleotides A198C, A387G) a…

Available Since

July 6, 2021

Availability

Academic Institutions and Nonprofits only

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pDEST17-cDAXX-0SB

Plasmid

#169730

Purpose

Bacterial expression of N-terminally 6His tagged cDAXX-0SB

Depositor

Tanja Mittag

Article

Bouchard et al Mol Cell. 2018 Oct 4;72(1):19-36.e8. doi

Insert

cDAXX-0SB (DAXX Human)

Tags

6 His

Expression

Bacterial

Mutation

Comprises residues 495-741 and has the major SB m…

Promoter

T7

Available Since

June 14, 2021

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmTral-LSm-R21EE23KR42EF44A-V5His6_C

Plasmid

#146071

Purpose

Insect Expression of DmTral-LSmR21EE23KR42EF44A

Depositor

Elisa Izaurralde

Article

Tritschler et al Mol Cell Biol. 2008 Nov . 28(21):6695-70

Insert

DmTral-LSmR21EE23KR42EF44A (tral Fly)

Expression

Insect

Mutation

R21E, E23K, R42E, F44A. Additionally, one silent …

Available Since

June 2, 2021

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmTral-R42EF44A_C

Plasmid

#146061

Purpose

Insect Expression of DmTral-R42EF44A

Depositor

Elisa Izaurralde

Article

Tritschler et al Mol Cell Biol. 2008 Nov . 28(21):6695-70

Insert

DmTral-R42EF44A (tral Fly)

Expression

Insect

Mutation

R42E, F44A. Additionally, two silent mutations A1…

Available Since

June 2, 2021

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmTral-LSm-R21EE23KS13AI15A-V5His6_C

Plasmid

#146049

Purpose

Insect Expression of DmTral-LSmR21EE23KS13AI15A

Depositor

Elisa Izaurralde

Article

Tritschler et al Mol Cell Biol. 2008 Nov . 28(21):6695-70

Insert

DmTral-LSmR21EE23KS13AI15A (tral Fly)

Expression

Insect

Mutation

S13A, I15A, R21E, E23K, one silent mutation A198C…

Available Since

June 2, 2021

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmTral-FDF-ADA_C

Plasmid

#146039

Purpose

Insect Expression of DmTral-FDF-ADA

Depositor

Elisa Izaurralde

Article

Tritschler et al Mol Cell. 2009 Mar 13. 33(5):661-8.

Insert

DmTral-FDF-ADA (tral Fly)

Expression

Insect

Mutation

FDF to ADA (407-409) mutation. Additionally, thre…

Available Since

June 2, 2021

Availability

Academic Institutions and Nonprofits only

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lenti MS2-P65-HSF1_Hygro

Plasmid

#61426

Purpose

lenti vector encoding the MS2-P65-HSF1 activator helper complex with a 2A Hygro resistance marker NOTE: A version of this plasmid with improved titer is available: Addgene plasmid #89308 lentiMPH v2

Depositor

Feng Zhang

Article

Konermann et al Nature. 2014 Dec 10. doi: 10.1038/nature

Insert

MS2-P65-HSF1_2A_Hygro (HSF1 Synthetic, Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

N55K in MS2

Promoter

EF1A

Available Since

Dec. 17, 2014

Availability

Academic Institutions and Nonprofits only

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MS2-P65-HSF1_GFP

Plasmid

#61423

Purpose

Expresses the MS2-P65-HSF1 activator helper complex with a 2A GFP. 3rd generation lentiviral vector.

Depositor

Feng Zhang

Article

Konermann et al Nature. 2014 Dec 10. doi: 10.1038/nature

Insert

MS2(N55K)-P65-HSF1_2A_GFP (HSF1 Synthetic, Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

N55K in MS2

Promoter

EF1A

Available Since

Dec. 15, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA3 c-SRC (WT)

Plasmid

#42202

Depositor

Robert Lefkowitz

Article

Luttrell et al Science. 1999 Jan 29. 283(5402):655-61.

Insert

c-SRC (SRC Human)

Expression

Mammalian

Mutation

L176F mutation compared to reference sequence NM_…

Promoter

CMV

Available Since

Feb. 8, 2013

Availability

Academic Institutions and Nonprofits only

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pDONR223 SARS-CoV-2 NSP6

Plasmid

#141260

Purpose

Gateway-compatible Entry vector, with insert of NSP6 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1

Depositor

Fritz Roth

Article

Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

Insert

NSP6 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1)

Use

Gateway Cloning

Mutation

Many synonymous changes due to codon optimization

Available Since

April 1, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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