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pT7-gRNA_zebrafish_mmp21

Plasmid

#72890

Purpose

plasmid to generate guideRNA against mmp21 gene in zebrafish

Depositor

Nicholas Katsanis

Article

Perles et al J Med Genet. 2015 Dec;52(12):840-7. doi:

Insert

zebrafish mmp21 (mmp21 Zebrafish)

Use

CRISPR

Promoter

T7 RNA pol

Available Since

Feb. 16, 2016

Availability

Academic Institutions and Nonprofits only

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Luc-Nanog-3'UTRmut

Plasmid

#63894

Purpose

Luciferase reporter of mouse Nanog 3'UTR with the miR-34 target site mutated

Depositor

Lin He

Article

Choi et al Nat Cell Biol. 2011 Oct 23;13(11):1353-6

Insert

Nanog homeobox (Nanog Mouse)

Expression

Mammalian

Available Since

June 19, 2015

Availability

Academic Institutions and Nonprofits only
pSMP-NR2F1_1

Plasmid

#36362

Depositor

George Daley

Article

Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

Insert

NR2F1 (NR2F1 Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

May 2, 2012

Availability

Academic Institutions and Nonprofits only
pBS C1QTNF3

Plasmid

#19221

Depositor

Cliff Tabin

Article

Bandyopadhyay et al Dev Biol. 2008 Sep 1;321(1):162-74. doi:

Insert

Collagenous repeat-containing (C1QTNF3 Chicken)

Expression

Bacterial

Available Since

Sept. 19, 2008

Availability

Academic Institutions and Nonprofits only
sspa-GCaMP6m

Plasmid

#67560

Purpose

short photoactivatable fluorescent calcium reporter

Depositor

Ehud Isacoff

Article

Berlin et al Nat Methods. 2015 Jul 13. doi: 10.1038/n

Insert

sspa-GCaMP6m

Expression

Mammalian

Mutation

K65T, I83V, G87S, Y92D, V115H, K118V, S187R, Y196…

Available Since

July 28, 2015

Availability

Academic Institutions and Nonprofits only
pBS FAM20C

Plasmid

#19214

Depositor

Cliff Tabin

Article

Bandyopadhyay et al Dev Biol. 2008 Sep 1;321(1):162-74. doi:

Insert

FAM20C (FAM20C Chicken)

Expression

Bacterial

Available Since

Sept. 19, 2008

Availability

Academic Institutions and Nonprofits only
pBR322 mCDK4 promoter

Plasmid

#16572

Depositor

Bert Vogelstein

Article

Hermeking et al Proc Natl Acad Sci U S A. 2000 Feb 29. 9

Insert

CDK4 promoter (Cdk4 Mouse)

Expression

Bacterial

Available Since

May 28, 2008

Availability

Academic Institutions and Nonprofits only
myc-SpELL pBacPAK8

Plasmid

#15318

Depositor

Joan Conaway, Ronald Conaway

Article

Banks et al J Biol Chem. 2007 Feb 23. 282(8):5761-9.

Insert

eleven nineteen lysine rich in leukemia (ell1 Fission Yeast)

Use

Baculovirus construction

Tags

Myc

Available Since

Aug. 15, 2007

Availability

Academic Institutions and Nonprofits only
pRS424-TUB2 - human TUBB2a Cterminal tail

Plasmid

#60396

Purpose

Expression of chimeric yeast beta tubulin (TUB2) - human beta2 tubulin Cterminal tail (TUBB2a) using a GAL promoter

Depositor

Ron Vale

Article

Sirajuddin et al Nat Cell Biol. 2014 Apr;16(4):335-44. do

Insert

TUB2

Expression

Yeast

Mutation

TUB2 amino acids 429 - end, replaced with human T…

Promoter

GAL

Available Since

Jan. 26, 2015

Availability

Academic Institutions and Nonprofits only
pSicoR-ADAM7i1

Plasmid

#59602

Purpose

Expression of shRNA against human ADAM7

Depositor

Miguel Ramalho-Santos

Article

Qin et al Cell. 2014 Jul 17;158(2):449-61. doi: 10

Insert

ADAM7 (ADAM7 Human)

Use

RNAi

Expression

Mammalian

Promoter

U6

Available Since

Sept. 26, 2014

Availability

Academic Institutions and Nonprofits only
pFA6a-GFP (F64L, S65T, V163A)-His3MX6

Plasmid

#53185

Purpose

chromosomal tagging with stable GFP

Depositor

John Pringle

Article

Ko et al Mol Biol Cell. 2007 Dec;18(12):5139-53.

Insert

HIs3MX6 (HIS3 Budding Yeast)

Use

Yeast genomic targeting

Tags

GFP (F64L, S65T, V163A)

Available Since

May 27, 2014

Availability

Academic Institutions and Nonprofits only
DCT.VV.Orf67

Plasmid

#12755

Purpose

This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

Depositor

Jack Bennink, Jon Yewdell

Article

Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

Insert

VV.Orf67

Expression

Mammalian

Available Since

Dec. 6, 2006

Availability

Academic Institutions and Nonprofits only
pWay21-MKP-1CH2A

Plasmid

#13473

Depositor

Anton Bennett

Article

Wu et al Mol Cell Biol. 2005 Jun . 25(11):4792-80

Insert

MKP-1CH2A (Dusp1 Mouse)

Tags

EGFP

Expression

Mammalian

Mutation

Contains aa 1-46

Available Since

Dec. 8, 2006

Availability

Academic Institutions and Nonprofits only
pWay21-MKP-1CH2B

Plasmid

#13474

Depositor

Anton Bennett

Article

Wu et al Mol Cell Biol. 2005 Jun . 25(11):4792-80

Insert

MKP-1CH2B (Dusp1 Mouse)

Tags

EGFP

Expression

Mammalian

Mutation

Contains aa 47-136

Available Since

Dec. 8, 2006

Availability

Academic Institutions and Nonprofits only
DCT.VV.Orf148

Plasmid

#12836

Purpose

This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

Depositor

Jack Bennink, Jon Yewdell

Article

Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

Insert

VV.Orf148

Expression

Mammalian

Available Since

Dec. 8, 2006

Availability

Academic Institutions and Nonprofits only
DCT.VV.Orf72

Plasmid

#12760

Purpose

This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

Depositor

Jack Bennink, Jon Yewdell

Article

Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

Insert

A34R

Expression

Mammalian

Available Since

Dec. 6, 2006

Availability

Academic Institutions and Nonprofits only
DCT.VV.Orf263

Plasmid

#12951

Purpose

This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

Depositor

Jack Bennink, Jon Yewdell

Article

Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

Insert

VV.Orf263

Expression

Mammalian

Available Since

Dec. 29, 2006

Availability

Academic Institutions and Nonprofits only
ZP61 Isthmin

Plasmid

#16881

Depositor

Randall Moon

Article

Weidinger et al Curr Biol. 2005 Mar 29. 15(6):489-500.

Insert

isthmin (ism1 Zebrafish)

Expression

Bacterial

Available Since

Feb. 1, 2008

Availability

Academic Institutions and Nonprofits only

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FLAG-SpEAF 60-251 pBacPAK8

Plasmid

#15321

Depositor

Joan Conaway, Ronald Conaway

Article

Banks et al J Biol Chem. 2007 Feb 23. 282(8):5761-9.

Insert

EAF1 (eaf1 Fission Yeast)

Use

Baculovirus construction

Tags

FLAG

Mutation

N terminal deletion lacking first 59 amino acids …

Available Since

Aug. 15, 2007

Availability

Academic Institutions and Nonprofits only
DCT.VV.Orf82

Plasmid

#12770

Purpose

This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

Depositor

Jack Bennink, Jon Yewdell

Article

Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

Insert

VV.Orf82

Expression

Mammalian

Available Since

Dec. 6, 2006

Availability

Academic Institutions and Nonprofits only
pBS Calponin3

Plasmid

#19194

Depositor

Cliff Tabin

Article

Bandyopadhyay et al Dev Biol. 2008 Sep 1;321(1):162-74. doi:

Insert

Calponin 3 (CNN3 Chicken)

Expression

Bacterial

Available Since

Sept. 19, 2008

Availability

Academic Institutions and Nonprofits only
DCT.VV.Orf152

Plasmid

#12840

Purpose

This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

Depositor

Jack Bennink, Jon Yewdell

Article

Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

Insert

VV.Orf152

Expression

Mammalian

Available Since

Dec. 13, 2006

Availability

Academic Institutions and Nonprofits only
pEvolvR-mCherry-enCas9(D10A)-PolI5M

Plasmid

#249060

Purpose

Expresses nucleus-localized enCas9 (D10A) fused to PolI5M and mCherry using a CMV promoter. Includes a GFP cassette flanked by BsmbI cut sites to knock in and coexpress user-defined gRNAs.

Depositor

John Dueber, David Schaffer

Article

Hurtado et al Nat Commun. 2025 Apr 19;16(1):3723. doi:

Insert

enCas9-PolI5M

Use

CRISPR

Expression

Mammalian

Available Since

Dec. 23, 2025

Availability

Academic Institutions and Nonprofits only

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pEvolvR-mCherry-enCas9(D10A)-PolI3M

Plasmid

#249061

Purpose

Expresses nucleus-localized enCas9 (D10A) fused to PolI3M and mCherry using a CMV promoter. Includes a GFP cassette flanked by BsmbI cut sites to knock in and coexpress user-defined gRNAs.

Depositor

John Dueber, David Schaffer

Article

Hurtado et al Nat Commun. 2025 Apr 19;16(1):3723. doi:

Insert

enCas9-PolI3M

Use

CRISPR

Expression

Mammalian

Available Since

Dec. 17, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
p-mCherry-dCas9-PolI5M

Plasmid

#249065

Purpose

Expresses nucleus-localized dCas9 fused to PolI5M and mCherry using a CMV promoter. Includes a GFP cassette flanked by BsmbI cut sites to knock in and coexpress user-defined gRNAs.

Depositor

John Dueber, David Schaffer

Article

Hurtado et al Nat Commun. 2025 Apr 19;16(1):3723. doi:

Insert

dCas9-PolI5M

Use

CRISPR

Expression

Mammalian

Available Since

Dec. 17, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
p-mCherry-enCas9(D10A)

Plasmid

#249063

Purpose

Expresses nucleus-localized enCas9 (D10A) fused to mCherry using a CMV promoter. Includes a GFP cassette flanked by BsmbI cut sites to knock in and coexpress user-defined gRNAs.

Depositor

John Dueber, David Schaffer

Article

Hurtado et al Nat Commun. 2025 Apr 19;16(1):3723. doi:

Insert

enCas9

Use

CRISPR

Expression

Mammalian

Available Since

Dec. 17, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pX459-Neo1 gRNA

Plasmid

#246575

Purpose

CRISPR vector co-expressing Cas9 and a mouse Neo1 gRNA

Depositor

Mario Vallon

Article

Heiden et al J Biol Chem. 2025 Sep 4:110682. doi: 10.

Insert

Neo1 gRNA (Neo1 Mouse)

Use

CRISPR

Promoter

U6

Available Since

Dec. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pX459-Nrp1 gRNA

Plasmid

#246578

Purpose

CRISPR vector co-expressing Cas9 and a mouse Nrp1 gRNA

Depositor

Mario Vallon

Article

Heiden et al J Biol Chem. 2025 Sep 4:110682. doi: 10.

Insert

Nrp1 gRNA (Nrp1 Mouse)

Use

CRISPR

Promoter

U6

Available Since

Dec. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pX459-Plxna1 gRNA

Plasmid

#246574

Purpose

CRISPR vector co-expressing Cas9 and a mouse Plxna1 gRNA

Depositor

Mario Vallon

Article

Heiden et al J Biol Chem. 2025 Sep 4:110682. doi: 10.

Insert

Plxna1 gRNA (Plxna1 Mouse)

Use

CRISPR

Promoter

U6

Available Since

Dec. 3, 2025

Availability

Academic Institutions and Nonprofits only

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huADF S3A

Plasmid

#245943

Purpose

Expressing a constitutively active human ADF

Depositor

James Bamburg

Article

Agnew et al J Biol Chem. 1995 Jul 21;270(29):17582-7

Insert

actin depolymerizing factor (ADF) (DSTN Human)

Expression

Mammalian

Mutation

S3A

Promoter

CMV

Available Since

Oct. 21, 2025

Availability

Academic Institutions and Nonprofits only

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TtEnd_E448A_pEHISTEV

Plasmid

#179733

Purpose

TtEnd_E448A_pEHISTEV is a mutant form of TtEnd5A_pEHISTEV vector. It expresess mutated TtEnd5A in which Glu448 has been mutated to Ala448.

Depositor

James Naismith

Article

Hussain et al Arch Biochem Biophys. 2025 Feb;764:11027

Insert

TtEnd5A

Tags

6xHis

Expression

Bacterial

Mutation

Glu448 has been mutated to Ala448.

Promoter

T7

Available Since

Oct. 14, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
TtEnd_E329A_pEHISTEV

Plasmid

#179734

Purpose

TtEnd_E329A_pEHISTEV is a mutant form of TtEnd5A_pEHISTEV vector. It expresess mutated TtEnd5A in which Glu329 has been mutated with Ala329

Depositor

James Naismith

Article

Hussain et al Arch Biochem Biophys. 2025 Feb;764:11027

Insert

TtEnd5A

Tags

6xHis

Expression

Bacterial

Mutation

Glu329 has been mutated to Ala329.

Promoter

T7

Available Since

Oct. 14, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
TtEnd_H280A_pEHISTEV

Plasmid

#179735

Purpose

TtEnd_H280A_pEHISTEV is a mutant form of TtEnd5A_pEHISTEV vector. It expresess mutated TtEnd5A in which His280 has been mutated with Ala280.

Depositor

James Naismith

Article

Hussain et al Arch Biochem Biophys. 2025 Feb;764:11027

Insert

TtEnd5A

Tags

6xHis

Expression

Bacterial

Mutation

His280 has been mutated to Ala280.

Promoter

T7

Available Since

Oct. 14, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
TtEnd_CDC_E329A

Plasmid

#179737

Purpose

TtEnd_CDC_E329A expresses TtEnd_CDC_pEHISTEV, in which Glu329 has been mutated with Ala329.

Depositor

James Naismith

Article

Hussain et al Arch Biochem Biophys. 2025 Feb;764:11027

Insert

TtEnd5A

Tags

6xHis

Expression

Bacterial

Mutation

Glu329 has been mutated with Ala329

Promoter

T7

Available Since

Oct. 14, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pWPI_GW_BioID2_T2A_Blast

Plasmid

#214831

Purpose

Destination vector for cloning BioID2 fusion proteins

Depositor

Hani Goodarzi

Article

Khoroshkin et al Nat Commun. 2024 Sep 9;15(1):7872. doi:

Type

Empty backbone

Use

Lentiviral

Tags

BioID2

Expression

Bacterial and Mammalian

Available Since

Aug. 12, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
LentiCRISPR-B_2xsgRNAs-RMRP (pAVA3583)

Plasmid

#239260

Purpose

Lentiviral vector expressing Cas9-P2A-Blasticidin and two sgRNAs targeting RMRP

Depositor

Andrei Alexandrov

Article

Che et al Cell Rep. 2025 Jun 24;44(6):115752. doi:

Insert

U6-driven sgRNA1 and 7SK-driven sgRNA2 targeting RMRP

Use

CRISPR and Lentiviral

Available Since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
LentiCRISPR-B_2xsgRNAs-RMRP (pAVA3584)

Plasmid

#239261

Purpose

Lentiviral vector expressing Cas9-P2A-Blasticidin and two sgRNAs targeting RMRP

Depositor

Andrei Alexandrov

Article

Che et al Cell Rep. 2025 Jun 24;44(6):115752. doi:

Insert

U6-driven sgRNA1 and 7SK-driven sgRNA2 targeting RMRP

Use

CRISPR and Lentiviral

Available Since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pET30a-GalK-USP-ET64

Plasmid

#234662

Purpose

Expression an ELP-tagged fusion enzyme of GalK and AtUSP

Depositor

Xianwei Liu

Article

Wu et al Chembiochem. 2025 Jan 13:e202401005. doi

Inserts

galactokinase
UDP-sugar pyrophosphorylase (USP Mustard Weed)

Tags

ELP

Expression

Bacterial

Available Since

July 24, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pSEVAb827

Plasmid

#217500

Purpose

pSEVAb (SEVA 3.1), Type IIS compatible plasmid with sfGFP under the BBa_J23119 promoter, pSEVAb82 backbone, RK2 ori

Depositor

Nico Claassens

Article

van den Brink et al ACS Synth Biol. 2025 Jun 5. doi: 10.1021

Insert

superfolder green fluorescent protein (sfGFP)

Use

Synthetic Biology

Mutation

S30R, Y39N, F64L, S65T, Q80R, F99S, N105T, Y145F,…

Available Since

July 17, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pSEVAb837

Plasmid

#217501

Purpose

pSEVAb (SEVA 3.1), Type IIS compatible plasmid with sfGFP under the BBa_J23119 promoter, pSEVAb83 backbone, pBBR1 ori

Depositor

Nico Claassens

Article

van den Brink et al ACS Synth Biol. 2025 Jun 5. doi: 10.1021

Insert

superfolder green fluorescent protein (sfGFP)

Use

Synthetic Biology

Mutation

S30R, Y39N, F64L, S65T, Q80R, F99S, N105T, Y145F,…

Available Since

July 17, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge

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