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Plasmid#153402PurposeYellow fluorescent protein CTAG codon optimised for Arabidopsis thalianaDepositorInsertYpet CTAG
UseTagsExpressionPlantMutationPromoterAvailable sinceOct. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET11b-MIF H62F
Plasmid#149445PurposeExpression of MIF H62F in E. coli for protein productionDepositorInsertMIF (MIF Human)
UseTagsExpressionBacterialMutationH62FPromoterAvailable sinceSept. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET11b-MIF H62A
Plasmid#149443PurposeExpression of MIF H62A in E. coli for protein productionDepositorInsertMIF (MIF Human)
UseTagsExpressionBacterialMutationH62APromoterAvailable sinceSept. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET11b-MIF H62Y
Plasmid#149442PurposeExpression of MIF H62Y in E. coli for protein productionDepositorInsertMIF (MIF Human)
UseTagsExpressionBacterialMutationH62YPromoterAvailable sinceSept. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET11b-MIF Y99G
Plasmid#149441PurposeExpression of MIF Y99G in E. coli for protein productionDepositorInsertMIF (MIF Human)
UseTagsExpressionBacterialMutationY99GPromoterAvailable sinceSept. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET28b-GFP-tglG
Plasmid#134679PurposeExpresses His6-GFP-TglG fusion in E. ColiDepositorInsertTglG
UseTagsHis6-GFPExpressionBacterialMutationPromoterT7Available sinceJuly 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSHDY*in_PpetE:BCD2:AgBIS:Flag
Plasmid#133971PurposeHeterologous, copper-inducible expression of (E)-α-bisabolene synthase from Abies grandis (AgBIS) in Synechocystis sp. PCC 6803. The AgBIS CDS is fused to a C-termial Flag-tag.DepositorInsertAgBIS:Flag
UseSynthetic BiologyTagsFlagExpressionBacterialMutationinserted gene is codon optimized for Synechocyst…PromoterPpetE:BCD2Available sinceApril 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSHDY*in_PpetE:BCD2:MrBBS:Strep
Plasmid#133973PurposeHeterologous, copper-inducible expression of (-)-α- bisabolol synthase from Matricaria recutita (MrBBS) in Synechocystis sp. PCC 6803. The MrBBS CDS is fused to a C-termial StrepII-tag.DepositorInsertMrBBS:StrepII
UseSynthetic BiologyTagsStrepIIExpressionBacterialMutationinserted gene is codon optimized for Synechocyst…PromoterPpetE:BCD2Available sinceApril 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET28a HAT epitope
Plasmid#129445Purposeexpression of HAT epitopeDepositorInsertHAT epitope
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET-FLAG-B-eSpCas9
Plasmid#126772PurposeExpression of increased fidelity Blackjack-eSpCas9 in bacterial cells. Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with higher fidelity than that of eSpCas9DepositorInsertB-eSpCas9
UseCRISPRTags3xFLAG, 6xHis, MBP, and NLSExpressionBacterialMutationK848A; K1003A; R1060A; amino acids 1005-1013 repl…PromoterT7Available sinceMarch 6, 2020AvailabilityAcademic Institutions and Nonprofits only