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pX459_LIG4_Exon3

Plasmid

#225363

Purpose

pX459 plasmid encoding the sgRNA protospacer sequence “5’-GCATAATGTCACTACAGATC-3’ to target the LIG4 gene.

Depositor

Sujatha Jagannathan

Article

Cortazar et al Methods Mol Biol. 2025;2863:397-417. doi

Insert

LIG4 (LIG4 Human)

Use

CRISPR

Promoter

U6

Available Since

July 1, 2025

Availability

Academic Institutions and Nonprofits only

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pXYB151

Plasmid

#239630

Purpose

Expression vector of AT3G20840

Depositor

Xiaohan Yang

Article

Liu et al Plant Biotechnol J. 2025 May;23(5):1640-

Insert

coding sequence of AT3G20840

Use

Synthetic Biology

Expression

Plant

Available Since

June 25, 2025

Availability

Academic Institutions and Nonprofits only

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pDL0_159

Plasmid

#210770

Purpose

S_Setaria viridis R1 coding sequence

Depositor

Dmitri Nusinow

Article

Lee et al Plant Biotechnol J. 2025 Dec 5. doi: 10.

Insert

SvR1

Use

Synthetic Biology

Expression

Plant

Available Since

June 25, 2025

Availability

Academic Institutions and Nonprofits only

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pDL0_217

Plasmid

#210772

Purpose

S_Setaria viridis C1 coding sequence

Depositor

Dmitri Nusinow

Article

Lee et al Plant Biotechnol J. 2025 Dec 5. doi: 10.

Insert

SvC1

Use

Synthetic Biology

Expression

Plant

Available Since

June 25, 2025

Availability

Academic Institutions and Nonprofits only

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pDL0_180

Plasmid

#210776

Purpose

C1_Setaria viridis C1 coding sequence

Depositor

Dmitri Nusinow

Article

Lee et al Plant Biotechnol J. 2025 Dec 5. doi: 10.

Insert

SvC1

Use

Synthetic Biology

Expression

Plant

Available Since

June 25, 2025

Availability

Academic Institutions and Nonprofits only

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pDL0_138

Plasmid

#210762

Purpose

SC_Setaria viridis R1 coding sequence

Depositor

Dmitri Nusinow

Article

Lee et al Plant Biotechnol J. 2025 Dec 5. doi: 10.

Insert

R1

Expression

Plant

Available Since

June 25, 2025

Availability

Academic Institutions and Nonprofits only

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pDL0_042

Plasmid

#210766

Purpose

T_CaMV 35S polyA signal sequence

Depositor

Dmitri Nusinow

Article

Lee et al Plant Biotechnol J. 2025 Dec 5. doi: 10.

Insert

35S terminator seqeunce

Use

Synthetic Biology

Expression

Plant

Available Since

June 25, 2025

Availability

Academic Institutions and Nonprofits only

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pXYB028

Plasmid

#239576

Purpose

Ribozyme split GFPuv expression

Depositor

Xiaohan Yang

Article

Liu et al Plant Biotechnol J. 2025 May;23(5):1640-

Insert

GFPuv coding sequence inserted with ribozyme

Use

Synthetic Biology

Expression

Plant

Available Since

June 24, 2025

Availability

Academic Institutions and Nonprofits only

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pXYB031

Plasmid

#239577

Purpose

Deactivated ribozyme split GFPuv expression

Depositor

Xiaohan Yang

Article

Liu et al Plant Biotechnol J. 2025 May;23(5):1640-

Insert

GFPuv coding sequence inserted with deactivated ribozyme

Use

Synthetic Biology

Expression

Plant

Available Since

June 23, 2025

Availability

Academic Institutions and Nonprofits only

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T7pro-tRNA-ITSIle intron-T7tVar1-pRS426

Plasmid

#239294

Purpose

Expresses the tRNAIle intron bearing the 5' GGGAGA initially transcribed sequence (ITS); Plasmid #2 of the tet-on overexpression system

Depositor

Anita Hopper

Article

Nostramo et al Mol Cell. 2025 Feb 20;85(4):726-741.e6.

Inserts

T7 promoter
ITS-tRNAIle intron
T7 terminator - Var1

Expression

Yeast

Available Since

June 23, 2025

Availability

Academic Institutions and Nonprofits only

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pYihI

Plasmid

#233088

Purpose

Expression of GST-YihI (wildtype sequence)

Depositor

Michael Downey

Article

Baijal et al mBio. 2025 Aug 13;16(8):e0050025. doi: 1

Insert

YihI

Tags

GST

Expression

Bacterial

Available Since

May 7, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pCMV-ABCB10-eYFP

Plasmid

#206038

Purpose

Express YFP in mitochondria

Depositor

Lufang Zhou

Article

Ernst et al Biophys J. 2019 Aug 20;117(4):631-645. d

Insert

EYFP

Tags

ABCB10 mitochondrial leading sequence

Expression

Mammalian

Available Since

April 24, 2025

Availability

Academic Institutions and Nonprofits only

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pTrcHisA-NLS-BFP-mEosEM

Plasmid

#218934

Purpose

Express the nuclear import substrate NLS-BFP-mEosEM.

Depositor

Siegfried Musser

Article

Sau et al Nature. 2025 Mar 19. doi: 10.1038/s41586

Insert

BFP-mEosEM fusion protein

Tags

6xHis and SV40 nuclear localization sequence (NLS…

Expression

Bacterial

Promoter

T7

Available Since

April 15, 2025

Availability

Academic Institutions and Nonprofits only

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GB4003

Plasmid

#215248

Purpose

Module for the constitutive expression of PIF6 and PhyB:VP16

Depositor

Diego Orzaez

Article

Calvache et al Plant Biotechnol J. 2024 Jan;22(1):37-47

Insert

PIF6-PhyB:VP16

Use

Synthetic Biology

Mutation

V234L in PIF6- please see dep. sequence, BsaI and…

Available Since

March 19, 2025

Availability

Academic Institutions and Nonprofits only

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JDW 1051 (pME_V5_GATA2_P2A_H2A_mCherry)

Plasmid

#232128

Purpose

A middle entry gateway clone containing V5 tagged human GATA2 and an H2A tagged mCherry, separated by a P2A peptide cleavage sequence.

Depositor

Joshua Wythe

Article

Cantu Gutierrez et al bioRxiv 2021.11.15.468651

Insert

GATA2 (GATA2 Human)

Use

Gateway cloning

Tags

V5

Available Since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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JDW 1034 (pME_V5_TCF7_P2A_H2A_mCherry)

Plasmid

#232124

Purpose

A middle entry gateway clone containing V5 tagged human TCF7 and an H2A tagged mCherry, separated by a P2A peptide cleavage sequence.

Depositor

Joshua Wythe

Article

Cantu Gutierrez et al bioRxiv 2021.11.15.468651

Insert

TCF7 (TCF7 Human)

Use

Gateway cloning

Tags

V5

Available Since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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JDW 1031 (pME_V5_LEF1_P2A_H2A_mCherry)

Plasmid

#232123

Purpose

A middle entry gateway clone containing V5 tagged human LEF1 and an H2A tagged mCherry, separated by a P2A peptide cleavage sequence.

Depositor

Joshua Wythe

Article

Cantu Gutierrez et al bioRxiv 2021.11.15.468651

Insert

LEF1 (LEF1 Human)

Use

Gateway cloning

Tags

V5

Available Since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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JDW 1060 (pME_V5_FOXF2_P2A_H2A_mCherry)

Plasmid

#232131

Purpose

A middle entry gateway clone containing V5 tagged human FOXF2 and an H2A tagged mCherry, separated by a P2A peptide cleavage sequence.

Depositor

Joshua Wythe

Article

Cantu Gutierrez et al bioRxiv 2021.11.15.468651

Insert

FOXF2 (FOXF2 Human)

Use

Gateway cloning

Tags

V5

Available Since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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JDW 1059 (pME_V5_GATA4_P2A_H2A_mCherry)

Plasmid

#232130

Purpose

A middle entry gateway clone containing V5 tagged human GATA4 and an H2A tagged mCherry, separated by a P2A peptide cleavage sequence.

Depositor

Joshua Wythe

Article

Cantu Gutierrez et al bioRxiv 2021.11.15.468651

Insert

GATA4 (GATA4 Human)

Use

Gateway cloning

Tags

V5

Available Since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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JDW 1036 (pME_V5_TCF7L2_P2A_H2A_mCherry)

Plasmid

#232126

Purpose

A middle entry gateway clone containing V5 tagged human TCF7L2 / TCF4 and an H2A tagged mCherry, separated by a P2A peptide cleavage sequence.

Depositor

Joshua Wythe

Article

Cantu Gutierrez et al bioRxiv 2021.11.15.468651

Insert

TCF7L2 / TCF4 (TCF7L2 Human)

Use

Gateway cloning

Tags

V5

Available Since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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pAV-H1promoter-2xTetO-Tornado-2xALFA-10xSunTag-Pseudoknot

Plasmid

#231593

Purpose

Express circular RNA translation reporter with 2xALFATag-10xSunTag-Pseudoknot under H1 promoter

Depositor

Marvin Tanenbaum

Article

Madern et al Cell. 2025 Jan 23:S0092-8674(25)00045-5.

Insert

Tornado-2xALFATag-10xSunTag-Pseudoknot sequence

Expression

Mammalian

Available Since

Jan. 31, 2025

Availability

Academic Institutions and Nonprofits only

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N-Terminal Halo XRCC4

Plasmid

#207541

Purpose

Homologous recombination donor to insert halo tag at the N terminal of the endogenous XRCC4 locus.

Depositor

Jens Schmidt

Article

Mikhova et al Nat Commun. 2024 Nov 23;15(1):10159. doi

Insert

HaloTag with internal PuroR cassette flanked by human XRCC4 locus sequences

Tags

HaloTag

Expression

Mammalian

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pVG329

Plasmid

#227771

Purpose

For genomic replacement of YOR1 gene in budding yeast

Depositor

Sahand Jamal Rahi

Article

Gligorovski et al Cell. 2026 Mar 6:S0092-8674(26)00167-4.

Insert

Genomic sequences flanking YOR1 in budding yeast (YOR1 Budding Yeast)

Expression

Yeast

Mutation

N/A

Available Since

Jan. 15, 2025

Availability

Academic Institutions and Nonprofits only

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pWR58

Plasmid

#202795

Purpose

integrating bioluminescene reporter for Cas9 activity in S. stipitis

Depositor

J. Brian Robertson

Article

Reichard et al mSphere. 2023 Aug 24;8(4):e0022423. doi:

Inserts

P-TDH3/coCBG(first portion)-YUM1
P-TEF1/ShBle/T-ACT1
YUM1-coCBG(last portion)/T-ADH2
URA3 and flanking sequence

Expression

Yeast

Available Since

Dec. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pGEMT-mMyh13CDS

Plasmid

#192853

Purpose

Plasmid to produce ISH probe for mMyh13 gene with CDS sequence

Depositor

Masafumi Inui

Article

Tanji et al Dev Growth Differ. 2022 Dec 14. doi: 10.

Insert

myosin heavy chain 13 (Myh13 Mouse)

Available Since

Dec. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pGEMT-mMyh13UTR

Plasmid

#192854

Purpose

Plasmid to produce ISH probe for mMyh13 gene with UTR sequence

Depositor

Masafumi Inui

Article

Tanji et al Dev Growth Differ. 2022 Dec 14. doi: 10.

Insert

myosin heavy chain 13 (Myh13 Mouse)

Available Since

Dec. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pYAMTr2GCsgSeLEU2

Plasmid

#224870

Purpose

Disruption of S. eubayanus type LEU2 genes

Depositor

Katsunori Suzuki

Article

Kiyokawa et al Appl Microbiol Biotechnol. 2024 Jul 12;1

Insert

pYAMTr2GC having the guide sequence SeLEU2 (DI49_0736 Budding Yeast)

Use

CRISPR

Expression

Yeast

Available Since

Oct. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pYAMTrGCsgScLEU2

Plasmid

#224869

Purpose

Disruption of S. cerevisiae type LEU2 genes

Depositor

Katsunori Suzuki

Article

Kiyokawa et al Appl Microbiol Biotechnol. 2024 Jul 12;1

Insert

pYAMTrGCas having the guide sequence ScLEU2 (LEU2 Budding Yeast)

Use

CRISPR

Expression

Yeast

Available Since

Oct. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pYAMTr2GCsgScLEU2

Plasmid

#224868

Purpose

Disruption of S. cerevisiae type LEU2 gene

Depositor

Katsunori Suzuki

Article

Kiyokawa et al Appl Microbiol Biotechnol. 2024 Jul 12;1

Insert

pYAMTr2GC having the guide sequence ScLEU2 (LEU2 Budding Yeast)

Use

CRISPR

Expression

Yeast

Available Since

Oct. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pLP-EV

Plasmid

#209973

Purpose

Contains Level 0 Part: mock coding sequence (EV) for the construction of Level 1 plasmids

Depositor

Thorsten Mascher

Article

Li et al ACS Synth Biol. 2024 Sep 20;13(9):2764-2

Insert

CDS (mock)

Expression

Bacterial

Available Since

Sept. 13, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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