We narrowed to 27,291 results for: Tat
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mCherry-CRY2-INPP5E(D556A)
Plasmid#79563Purposeexpression of mCherry-tagged CRY2PHR fused to catalytically dead INPP5EDepositorInsertINPP5E (INPP5E Human)
UseTagsCRY2 (photolyase homology region domain of Arabid…ExpressionMammalianMutationC-terminal region, aa 214_644, C641A mutation to …PromoterCMVAvailable sinceJuly 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_RARA_WT
Plasmid#82886PurposeGateway Donor vector containing RARA, part of the Target Accelerator Plasmid Collection.DepositorInsertNM_001145301 (RARA Human)
UseGateway entry vectorTagsExpressionMutationPromoterNoneAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_POLR2I_WT_V5
Plasmid#82955PurposeGateway Donor vector containing POLR2I, used as an over expression control for gene expression analysis. Part of the Target Accelerator Plasmid Collection.DepositorInsertPOLR2I (POLR2I Human)
UseGateway entry vectorTagsExpressionMutationPromoterNoneAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
mVenus-proα2(I)-G610C
Plasmid#119839PurposeExpresses mouse Type I procollagen α2 chain (Col1a2) with Gly610Cys mutation and mVenus between signal sequence and exon 6DepositorInsertType I procollagen α2 chain (Col1a2 Mouse)
UseTagsmVenusExpressionMammalianMutationCol1a2 exons 2-5 replaced by fluorescent tag, Gly…PromoterCMVAvailable sinceJan. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-SLC16A10_STOP
Plasmid#161112PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.DepositorInsertSLC16A10 (SLC16A10 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 21, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pcDNA3-CARD14-G117S
Plasmid#69920PurposeExpresses mutant FLAG-CARD14 in mammalian cellsDepositorInsertCARD14 isoform 3 (CARD14 Human)
UseTagsFLAGExpressionBacterial and MammalianMutationG117SPromoterCMVAvailable sinceFeb. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
MSCV-BRD9_N216A-PGK-Puro-IRES-GFP
Plasmid#75116PurposeRetroviral expression plasmid encoding a human BRD9 mutant carrying the N216A single amino acid mutationDepositorInsertBRD9 (BRD9 Human)
UseRetroviralTagsExpressionMutationsingle amino acid mutation N216APromoterAvailable sinceMay 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMiR-RECK-3'UTR-miR-21-mut
Plasmid#53693PurposeLuciferase reporter assay for RECK 3'UTR that has point mutations on miR-21 binding siteDepositorInsertRECK-3'UTR (RECK Human)
UseLuciferaseTagsExpressionMutationnucleotide sequence 1083-1086 is mutated to TTCGPromoterAvailable sinceJuly 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
MSCV-HA-Nr4a1-AA
Plasmid#160942PurposeGeneration of retrovirus for the overexpression of Nr4a1 mutant (287C 288EAA)DepositorInsertNr4a1 (Nr4a1 Mouse)
UseRetroviralTagsHA tagExpressionMammalianMutationChange Cys 287 Glu 288 into Ala AlaPromoterMSCV-LTRAvailable sinceDec. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_STC2_WT
Plasmid#82883PurposeGateway Donor vector containing STC2, part of the Target Accelerator Plasmid Collection.DepositorInsertSTC2 (STC2 Human)
UseGateway entry vectorTagsExpressionMutationPromoterNoneAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFUSEss-CHIg-mM-Asn209Ala_M18
Plasmid#91733PurposeExpression plasmid coding for mutated heavy chain of mouse IgM antibody specific to antigen B of the ABO blood group system, clone M18. Mutation: Asn209Ala (EU numbering).DepositorInsertimmunoglobulin heavy constant mu (Ighm Mouse)
UseTagsExpressionMammalianMutationAsn209 changed to Ala (EU numbering)PromoterhEF1-HTLV promAvailable sinceJune 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
LADL Bridge + Target Zfp462-Klf4SE
Plasmid#127668PurposeEncodes for CRY2-HA and mCherry proteins expressed from the EF1a promoter; 2 gRNAs targeting the Zfp462 promoter and 2 gRNAs targeting the Klf4 SE expressed from their individual hU6 promotersDepositorInsertCRY2-HA-2A-mCherry and gRNAs 129, 135, 115 and 117
UseCRISPRTagsHAExpressionMammalianMutationPromoterEF1a and hU6Available sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
Flag-KLF6-4D (1054)
Plasmid#49490Purposeexpresses human Flag tagged KLF6 with 4D mutationDepositorInsertKFL6-4D (KLF6 Human)
UseTagsFlagExpressionMammalianMutationthree serines and one tyrosine mutated to aspart…PromoterCMVAvailable sinceFeb. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pRK5-Myc-PSK2 (1-416)
Plasmid#197123PurposeExpression of Myc-tagged PSK2 (aa1-416) / TAOK1 (aa1-416) in mammalian cellsDepositorInsertTAOK1 (aa1-416) (TAOK1 Human)
UseTagsMycExpressionMammalianMutationDeleted C-terminusPromoterSV40Available sinceMarch 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
MAPK8 gRNA (BRDN0001146407)
Plasmid#78048Purpose3rd generation lentiviral gRNA plasmid targeting human MAPK8DepositorInsertMAPK8 (MAPK8 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_STC2_WT_V5
Plasmid#82957PurposeGateway Donor vector containing STC2, used as an over expression control for gene expression analysis. Part of the Target Accelerator Plasmid Collection.DepositorInsertSTC2 (STC2 Human)
UseGateway entry vectorTagsExpressionMutationPromoterNoneAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_RBM45_WT_V5
Plasmid#82968PurposeGateway Donor vector containing RBM45, used as an over expression control for gene expression analysis. Part of the Target Accelerator Plasmid Collection.DepositorInsertRBM45 (RBM45 Human)
UseGateway entry vectorTagsExpressionMutationPromoterNoneAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
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pCW-ND-Cdt1-HA-Puro
Plasmid#193767PurposeDoxycyline-inducible (TetOn) human Cdt1 (SV40 NLS localization and C-terminal HA tag) with mutations which abroggate its S phase degradation, expressed from all-in-one pCW vector (TetOn + rtTA)DepositorInsertND-Cdt1-HA (CDT1 Human)
UseLentiviralTagsHAExpressionMutationamino acids 1-19 deleted, ΔCy mutation (aa68-70 t…PromoterTREAvailable sinceJan. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRetroX GFP Mps1 DK
Plasmid#63703PurposeInducible expression of GFP-Mps1 DK fusion in mammalian cellsDepositorInsertMps1 (TTK Human)
UseRetroviralTagsEGFPExpressionMammalianMutationdeletion of aa 47-156, kinetochore binding domainPromotertight TRE promoterAvailable sinceApril 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-DDX11-Flag-KAE
Plasmid#120728PurposeExpression of a Flag-tagged version of DDX11 KAE mutant in mammalian cellsDepositorInsertDDX11 KAE mutant (DDX11 Human)
UseTags3x FLAGExpressionMammalianMutationSubstitution of E201 and Y202 with K and APromoterCMVAvailable sinceFeb. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_PSMD9_WT_V5
Plasmid#82950PurposeGateway Donor vector containing PSMD9, used as an over expression control for gene expression analysis. Part of the Target Accelerator Plasmid Collection.DepositorInsertPSMD9 (PSMD9 Human)
UseGateway entry vectorTagsExpressionMutationPromoterNoneAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
mCerulean-proα2(I)-G610C
Plasmid#119837PurposeExpresses mouse Type I procollagen α2 chain (Col1a2) with Gly610Cys mutation and mCerulean between signal sequence and exon 6DepositorInsertType I procollagen α2 chain (Col1a2 Mouse)
UseTagsmCeruleanExpressionMammalianMutationCol1a2 exons 2-5 replaced by fluorescent tag, Gly…PromoterCMVAvailable sinceJan. 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
Desmoplakin I tension sensor (F40-based)
Plasmid#118725PurposeThe F40-based human desmoplakin I tension sensor detects forces in the range of 1-6 pN by changes in FRET between YPet(short) and mCherry.DepositorInserthuman Desmoplakin I-[YPet(short)-F40-mCherry] (internal-1952) (DSP Human)
UseRetroviralTagsYPet(short)-F40-mCherryExpressionMammalianMutationinserted F40-based tension sensor module after aa…PromoterCMVAvailable sinceDec. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_NOSIP_WT_V5
Plasmid#82970PurposeGateway Donor vector containing NOSIP, used as an over expression control for gene expression analysis. Part of the Target Accelerator Plasmid Collection.DepositorInsertNOSIP (NOSIP Human)
UseGateway entry vectorTagsExpressionMutationPromoterNoneAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1α1.1-Chronos-GFP
Plasmid#122098PurposeAAV-mediated expression of Chronos-GFP under the EF1α promoter (1.1kb short version). Using SV40 pA signal.DepositorInsertChronos-GFP
UseAAVTagsGFPExpressionMammalianMutationPromoterEF1α (1.1 kb short version)Available sinceAug. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCJ199
Plasmid#162672PurposepET-21b(+) based plasmid for expression of the putative MHET hydrolase from Comamonas thiooxydans (Genbank WP_080747404.1) with C-terminal His tag, codon optimized for expression in E. coli K12.DepositorInsertPutative MHET hydrolase from Comamonas thiooxydans (Genbank WP_080747404.1) with signal peptide
UseTagsHisExpressionBacterialMutationcodon optimized for expression in E. coli K12PromoterT7/lacAvailable sinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBiex1-SYNAC
Plasmid#82710PurposeSynthetic soluble adenylyl cyclase FRET sensor and cAMP generatorDepositorUseTagsFLAG, mCerulean, and mCitrineExpressionBacterial and InsectMutationPromoterT7Available sinceNov. 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEF5/FRT/DEST-3xHA/mGli3/Flag P5-6A
Plasmid#51249PurposeEncodes N-3xHA-TEV-mouseGli3-Flag-C; amino acids 980,1006 mutated to AlaDepositorInsertGli3 (Gli3 Mouse)
UseFlpin systemTags3xHA-TEV and FlagExpressionMammalianMutationamino acids 980,1006 mutated to AlaPromoterEF1aAvailable sinceFeb. 28, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-DDX11-Flag-KAK
Plasmid#120729PurposeExpression of a Flag-tagged version of DDX11 KAK mutant in mammalian cellsDepositorInsertDDX11 KAK mutant (DDX11 Human)
UseTags3x FLAGExpressionMammalianMutationSubstitution of E201, Y202 and E203 with K, A and…PromoterCMVAvailable sinceFeb. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
MAPK9 gRNA (BRDN0001146002)
Plasmid#76719Purpose3rd generation lentiviral gRNA plasmid targeting human MAPK9DepositorInsertMAPK9 (MAPK9 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pQC MCS IRES G418
Plasmid#110344Purposeγ-Retroviral transfer vector for cloning and expressing your gene of interest. IRES-driven Geneticin selection.DepositorTypeEmpty backboneUseRetroviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-CARD14-D176H
Plasmid#69919PurposeExpresses mutant FLAG-CARD14 in mammalian cellsDepositorInsertCARD14 isoform 3 (CARD14 Human)
UseTagsFLAGExpressionBacterial and MammalianMutationD176HPromoterCMVAvailable sinceFeb. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
3294_pETcon-SARS2-RBD_Omicron-BA1
Plasmid#184408Purposeyeast surface display of the SARS-CoV-2 Omicron BA.1 variant RBDDepositorInsertSARS-CoV-2 Omicron BA.1 Spike receptor binding domain (RBD) (S SARS-CoV-2 virus)
UseTagsHA and c-MycExpressionYeastMutationG339D+S371L+S373P+S375F+K417N+N440K+G446S+S477N+T…PromoterAvailable sinceMay 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
mApple-proα2(I)-G610C
Plasmid#119841PurposeExpresses mouse Type I procollagen α2 chain (Col1a2) with Gly610Cys mutation and mApple between signal sequence and exon 6DepositorInsertType I procollagen α2 chain (Col1a2 Mouse)
UseTagsmAppleExpressionMammalianMutationCol1a2 exons 2-5 replaced by fluorescent tag, Gly…PromoterCMVAvailable sinceJan. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
Bxb1Donor_G102H_ADRB2_LucReporter
Plasmid#129794Purposeencodes a donor vector for recombination with the Bxb1 recombinase with variant G102H of the Beta-2 Adrenergic Receptor and a cAMP-responsive luciferase reporter geneDepositorInsertADRB2 (ADRB2 Human)
UseSynthetic BiologyTags3x FLAGExpressionMammalianMutationG102HPromoterN/AAvailable sinceSept. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEF5/FRT/DEST-3xHA/mGli3/Flag P1-4A
Plasmid#51247PurposeEncodes N-3xHA-TEV-mouseGli3-Flag-C; amino acids 849,865,877,907 mutated to AlaDepositorInsertGli3 (Gli3 Mouse)
UseFlpin systemTags3xHA-TEV and FlagExpressionMammalianMutationamino acids 849,865,877,907 mutated to AlaPromoterEF1aAvailable sinceFeb. 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGL3-Enhancer-ZC3H12D_PromoterMUT
Plasmid#153068PurposeZC3H12D promoter region carrying mutations in BHLHE40 binding sites, cloned upstream of luciferase reporterDepositorInsertZC3H12D promoter region mutated in BHLHE40 binding sites (ZC3H12D Human)
UseLuciferaseTagsExpressionMammalianMutationMutagenesis to disrupt 3 BHLHE40 binding sites. N…PromoterNoneAvailable sinceSept. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV2-TBG-FLAG-mRIPK1-S321A
Plasmid#115342PurposeLiver-specific adeno-associated viral delivery and mammalian expression of Flag-mRIPK1-S321ADepositorInsertRIPK1 (Ripk1 Mouse)
UseAAVTagsFlagExpressionMammalianMutationS321APromoterAvailable sinceSept. 11, 2018AvailabilityAcademic Institutions and Nonprofits only
