We narrowed to 40,651 results for: tro

1. pTH847-gst-optsc

   Plasmid

   #96908

   Purpose

   Plasmid for yeast expression of GST encoded by preferred codons

   Depositor

   Tobias von der Haar

   Article

   Jossé et al bioRxiv 200006

   Insert

   yeast codon optimised glutathione-S transferase
   

   Use

   Tags

   8xhistidine

   Expression

   Yeast

   Mutation

   Promoter

   TDH3

   Available since

   March 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCali2

   Plasmid

   #86119

   Purpose

   Multiplex temperature-gated expression of mWasabi and mCherry reporters

   Depositor

   Mikhail Shapiro

   Article

   Piraner et al Nat Chem Biol. 2017 Jan;13(1):75-80. doi

   Inserts

   TlpA36
   mWasabi
   TcI
   mCherry
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   A67T and D341A, P60L, D135V, K187R, K202I, L208Q

   Promoter

   pR/pL, pR/pL, pLacI, pTlpA, and pTlpA, pLacI

   Available since

   Feb. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pAAV-anti-GFP(LaG16) PAGER(Gi)

   Plasmid

   #230008

   Purpose

   Expresses anti-GFP PAGER(Gi) in mammalian cells

   Depositor

   Alice Ting

   Article

   Kalogriopoulos et al Nature. 2024 Dec 4. doi: 10.1038/s41586-

   Insert

   MT1-LaG16-TEVcs-ALFA-hM4D
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pQCXIZ-turq-3xFLAG-TREX1

   Plasmid

   #164232

   Purpose

   retroviral plasmid for turq-3xFLAG-TREX1

   Depositor

   John Maciejowski

   Article

   Mohr et al Mol Cell. 2021 Jan 12. pii: S1097-2765(2

   Insert

   TREX1 (TREX1 Human)
   

   Use

   Retroviral

   Tags

   FLAG and mturquoise

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. 3XHA-PP2A-Ba

   Plasmid

   #195229

   Purpose

   Retroviral vector containing HA tagged PP2A

   Depositor

   David Bryant, Keith Mostov

   Article

   Bryant et al Dev Cell. 2014 Oct 27;31(2):171-87. doi:

   Insert

   PPP2R2A (PPP2R2A Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pAG426GPD-ccdB-yeastSMASh-YFP

   Plasmid

   #68854

   Purpose

   Expresses SMASh tag fused to YFP at N terminus in yeast

   Depositor

   Michael Lin

   Article

   Chung et al Nat Chem Biol. 2015 Jul 27. doi: 10.1038

   Insert

   yeastSMASh-YFP
   

   Use

   Tags

   SMASh tag

   Expression

   Yeast

   Mutation

   Promoter

   GPD

   Available since

   Sept. 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCS2-mCherry-Rab11a

   Plasmid

   #184035

   Purpose

   Expresses mCherry Rab11a in mammalian cells and zebrafish embryos

   Depositor

   Heidi Hehnly

   Article

   Krishnan et al Life Sci Alliance. 2022 Mar 18;5(7). pii

   Insert

   Rab11a
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV IE94

   Available since

   May 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pET28a-PACT

   Plasmid

   #50352

   Purpose

   bacterial expression of PACT

   Depositor

   Kumiko Ui-Tei

   Article

   Takahashi et al PLoS One. 2013 May 2;8(5):e63434. doi: 1

   Insert

   PACT (PRKRA Human)
   

   Use

   Tags

   His and Myc

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Jan. 15, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. p3E-MCS

   Plasmid

   #75174

   Purpose

   Multiple cloning site for insertion of gene of interest for C-terminal fusions.

   Depositor

   Nicholas Cole

   Article

   Don et al Zebrafish. 2016 Sep 15.

   Insert

   Multiple Cloning Site
   

   Use

   Three prime entry vector for multisite gateway th…

   Tags

   Expression

   Mutation

   Promoter

   n/a

   Available since

   Oct. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. L1_CsR-Ck1

    Plasmid

    #136124

    Purpose

    L1 in position 1 (next to LB in L2 construct), for CsR

    Depositor

    Jim Haseloff

    Article

    Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

    Insert

    p5-35Sx2:mALS_3t35S
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    BsaI/ SapI domesticated

    Promoter

    Available since

    Nov. 30, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

11. pAAV-anti-TNFalpha(Ozoralizumab) PAGER(FL)

    Plasmid

    #230024

    Purpose

    Expresses anti-TNFalpha PAGER(FL) in mammalian cells

    Depositor

    Alice Ting

    Article

    Kalogriopoulos et al Nature. 2024 Dec 4. doi: 10.1038/s41586-

    Insert

    pAAV-MT3-Ozoralizumab-TEVcs-ALFA-hM4DxcpGFP
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pACT5FVMF(f)

    Plasmid

    #110549

    Purpose

    Control plasmid for titering FV by qPCR to determine the genome-containing particle number. This qPCR protocol is described in Hendrie et al., 2008

    Depositor

    David Russell

    Article

    Hendrie et al Mol Ther. 2008 Mar;16(3):534-40. doi: 10

    Insert

    EGFP, FV LTRs
    

    Use

    Qpcr control for titering foamy virus

    Tags

    Expression

    Mutation

    Promoter

    promoter: MLV LTR

    Available since

    June 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pUAP4

    Plasmid

    #136079

    Purpose

    Acceptor plasmid for L0 parts. Clone L0 parts into pUAP4 with SapI, lacZ blue-white screening. Release parts to assemble transcription units with BsaI.

    Depositor

    Jim Haseloff

    Article

    Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

    Insert

    
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Nov. 30, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

14. pCMV-Flag-YAP-S94A

    Plasmid

    #33102

    Depositor

    Kunliang Guan

    Article

    Zhao et al Genes Dev. 2008 Jul 15. 22(14):1962-71.

    Insert

    YAP (YAP1 )
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    S94A

    Promoter

    Available since

    March 21, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. L1_UBE2:mS-Lt-Ck3

    Plasmid

    #136131

    Purpose

    L1 in position 3, mark cell outline with mScarletI

    Depositor

    Jim Haseloff

    Article

    Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

    Insert

    p5-MpUBE2:mScarletI-Lti6b_3tNos-35S
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    BsaI/ SapI domesticated

    Promoter

    Available since

    Nov. 30, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

16. Cdk6-HA

    Plasmid

    #1868

    Depositor

    Sander van den Heuvel

    Article

    van den Heuvel et al Science 1993 Dec 24;262(5142):2050-4.

    Insert

    Cdk6 (CDK6 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    D224Y

    Promoter

    Available since

    Feb. 14, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pEGFP(C3)-Nup153-N

    Plasmid

    #64318

    Purpose

    GFP-tagged mammalian expression of N-terminal domain of Nup153

    Depositor

    Birthe Fahrenkrog

    Article

    Duheron et al Nucleus. 2014;5(6):601-12. doi: 10.4161/

    Insert

    Nup153 (NUP153 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    N-terminal domain

    Promoter

    CMV

    Available since

    April 23, 2015

    Availability

    Academic Institutions and Nonprofits only

18. PB_iASCL1_P2A_DLX2_T2A_mCherry_Puro

    Plasmid

    #168806

    Purpose

    PiggyBac transposon construct with doxycycline inducible ASCL1, DLX2 + mCherry expression

    Depositor

    Prashant Mali

    Article

    Dailamy et al Stem Cell Reports. 2021 Sep 8. pii: S221

    Insert

    ASCL1-P2A-DLX2-T2A-mCherry
    

    Use

    Synthetic Biology; Piggybac

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE

    Available since

    Aug. 31, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pMK345 (Hygro-P2A-mAID-mClover)

    Plasmid

    #121179

    Purpose

    N-terminal tagging

    Depositor

    Masato Kanemaki

    Article

    Yesbolatova et al Methods. 2019 Apr 23. pii: S1046-2023(18

    Insert

    Hygro-P2A-mAID-mClover
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. 2xMARS-nGFP

    Plasmid

    #205235

    Purpose

    Expresses two repeats of PLEKHA5 aa 143-271 (K163A and R164A) fused to a HA-tagged GFP nanobody in mammalian cells

    Depositor

    Jeremy Baskin

    Insert

    2xPLEKHA5(143-271)-K163A/R164A-HA-nGFP (PLEKHA5 Human)
    

    Use

    Tags

    HA, Nuclear Export Sequence, mScarlet-i, and nGFP…

    Expression

    Mammalian

    Mutation

    amino acids 143-271 of PLEKHA5 (GenBank reference…

    Promoter

    CMV

    Available since

    July 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pmGFP-P2A-K(AAA)12-P2A-RFP

    Plasmid

    #105687

    Purpose

    Double fluorescent stalling reporter K(AAA)12

    Depositor

    Ramanujan Hegde

    Article

    Juszkiewicz et al Mol Cell. 2017 Feb 16;65(4):743-750.e4.

    Inserts

    P2A-3xFLAG-VHPbeta
    K(AAA)12
    P2A-mCherry
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV (from vector)

    Available since

    Feb. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pCk4_spacer

    Plasmid

    #136074

    Purpose

    Use when making even level constructs (L2,...) if pCk4 position (position 4) has no construct assigned

    Depositor

    Jim Haseloff

    Article

    Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

    Insert

    Odd spacer
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    BsaI/ SapI domesticated

    Promoter

    Available since

    Nov. 30, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

23. Cdk5-DN-HA

    Plasmid

    #1873

    Depositor

    Sander van den Heuvel

    Article

    van den Heuvel et al Science 1993 Dec 24;262(5142):2050-4.

    Insert

    Cdk5 (CDK5 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    D145N, dominant negative

    Promoter

    Available since

    March 14, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pDA077

    Plasmid

    #131388

    Purpose

    L1RP expression

    Depositor

    Kathleen Burns

    Article

    Ardeljan et al Nat Struct Mol Biol. 2020 Feb;27(2):168-

    Insert

    L1RP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. AAV.hSyn.GLPLight-ctr

    Plasmid

    #187468

    Purpose

    Expresses GLPLight-ctr in mammalian cells

    Depositor

    Tommaso Patriarchi

    Article

    Duffet et al Elife. 2023 Jun 2;12:RP86628. doi: 10.75

    Insert

    GLPLight-ctr
    

    Use

    AAV

    Tags

    Flag tag

    Expression

    Mutation

    Promoter

    human Synapsin-1

    Available since

    Feb. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pMXspuro-GFP-NBR1

    Plasmid

    #74202

    Purpose

    Retroviral expression of GFP-NBR1

    Depositor

    Jayanta Debnath

    Article

    Kenific et al J Cell Biol. 2016 Feb 29;212(5):577-590.

    Insert

    neighbor of BRCA1 gene 1 (NBR1 Human)
    

    Use

    Retroviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 25, 2016

    Availability

    Academic Institutions and Nonprofits only

27. 3TERM_Nos-35S

    Plasmid

    #136116

    Purpose

    3TERM after CDS/ CDS12-CTAG

    Depositor

    Jim Haseloff

    Article

    Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

    Insert

    3TERM_Nos-35S (A. tumefaciens, CaMV)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    BsaI/ SapI domesticated

    Promoter

    Available since

    Nov. 30, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

28. CRY2-GFP-homer1c

    Plasmid

    #89442

    Purpose

    Localizes CRY2 to excitatory synapses

    Depositor

    Matthew Kennedy

    Article

    Sinnen et al Neuron. 2017 Feb 8;93(3):646-660.e5. doi

    Insert

    CRY2-GFP-homer1c
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Contains Full length CRY2

    Promoter

    CAG

    Available since

    April 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pDEST_6xUAS:mNeonGreen-Rab5a

    Plasmid

    #192355

    Purpose

    zfRabaa tagged with mNeonGreen, expressed under the UAS promoter

    Depositor

    Francesca Peri

    Article

    Moller et al Elife. 2022 Nov 18;11:e82094. doi: 10.75

    Insert

    Rab5a (rab5aa Zebrafish)
    

    Use

    Tags

    mNeonGreen

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. CAG-Flex-TCB

    Plasmid

    #48332

    Purpose

    Cre-dependant TVA-mCherry fusion

    Depositor

    Liqun Luo

    Article

    Miyamichi et al Neuron. 2013 Dec 4;80(5):1232-45. doi: 1

    Insert

    TVA receptor mCherry
    

    Use

    AAV

    Tags

    mCherry fusion to TVA receptor

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Dec. 6, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pCk3_spacer

    Plasmid

    #136073

    Purpose

    Use when making even level constructs (L2,...) if pCk3 position (position 3) has no construct assigned

    Depositor

    Jim Haseloff

    Article

    Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

    Insert

    Odd spacer
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    BsaI/ SapI domesticated

    Promoter

    Available since

    Nov. 30, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

32. pORE303N

    Plasmid

    #194438

    Purpose

    CRISPR, Cas9 driven by double 35S, nptII for plant selection, KpnI site for gRNA insertion

    Depositor

    Chung-Jui Tsai

    Article

    Ortega et al New Phytol. 2022 Nov 16. doi: 10.1111/np

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    2x35S

    Available since

    Jan. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pMK349 (BSD-P2A-mAID-mCherry)

    Plasmid

    #121183

    Purpose

    N-terminal tagging

    Depositor

    Masato Kanemaki

    Article

    Yesbolatova et al Methods. 2019 Apr 23. pii: S1046-2023(18

    Insert

    BSD-P2A-mAID-mCherry
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. Cdk2-DN-HA

    Plasmid

    #1885

    Depositor

    Sander van den Heuvel

    Article

    van den Heuvel et al Science 1993 Dec 24;262(5142):2050-4.

    Insert

    Cdk2 (CDK2 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    D146N, dominant negative

    Promoter

    Available since

    Feb. 14, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. p32Fn

    Plasmid

    #158153

    Purpose

    Construct conventional Cpf1 vectors that uses U3p and UBI10p to express single crRNA (or PTC/CA) and LbCpf1, respectively. For plant genome editing.

    Depositor

    Kabin Xie

    Article

    Ding et al Mol Plant. 2018 Apr 2;11(4):542-552. doi

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Rice codon optimized

    Promoter

    Available since

    Jan. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. P70a-deGFP-sc101-kan

    Plasmid

    #184841

    Purpose

    Expression of deGFP

    Depositor

    Chase Beisel

    Article

    Wandera et al Mol Cell. 2022 May 24. pii: S1097-2765(2

    Insert

    green fluorescent protein
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    promoter OR2-OR1

    Available since

    Oct. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pET51b(+)_EGFP-LAMA-R59 (for ALFA-tag)

    Plasmid

    #136627

    Purpose

    Plasmid encoding the bacterial expression of LAMA-R59 for ALFA-tag as an EGFP-fusion

    Depositor

    Kai Johnsson

    Article

    Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

    Insert

    EGFP-LAMA-R59
    

    Use

    Tags

    Hisx10 and Strep

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pCMV7 mitoDendra2

    Plasmid

    #157757

    Purpose

    Expression of the fluorescent protein Dendra2 targeted to mitochondria

    Depositor

    Marc Tramier

    Article

    Bertolin et al eLife. 2018 Aug 2;7. pii: 38111. doi: 10

    Insert

    Dendra2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    MTS coxVIII +Dendra2

    Promoter

    CMV

    Available since

    Feb. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. miniCMV eGFP hPGK BSD

    Plasmid

    #188519

    Purpose

    Backbone for CRISPR activation (CRISPRa) isogenic PAM testing

    Depositor

    Isaac Hilton

    Article

    Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pCAG-ChR2-mRuby2-ST

    Plasmid

    #109125

    Purpose

    Cation channelrhodopsin ChR2 fused to mRuby2 fluorophore and targeted to the neuronal soma and proximal dendrites

    Depositor

    Hillel Adesnik

    Article

    Mardinly et al Nat Neurosci. 2018 Apr 30. pii: 10.1038/

    Insert

    ChR2-mRuby2-ST
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    June 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge