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pHCKan-pstSp-GFPuv

Plasmid

#139077

Purpose

Low phosphate inducible promoter pstSp for GFP expression

Depositor

Michael Lynch

Article

Moreb et al ACS Synth Biol. 2020 Jun 19;9(6):1483-14

Insert

pstSp-GFPuv

Expression

Bacterial

Available Since

Sept. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pFB HTB_hMSH6_T1219D

Plasmid

#135466

Purpose

Expression human MSH6_T1219D mutant protein using baculovirus

Depositor

Peggy Hsieh

Article

Geng et al Anal Biochem. 2011 Jun 15;413(2):179-84.

Insert

human MSH6_T1219D (MSH6 Human)

Tags

His-tag

Expression

Insect

Mutation

changed threonine to aspartic acid

Available Since

Jan. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pNIC28-BSA4-S15

Plasmid

#127821

Purpose

Production of recombinant human ribosomal protein S15

Depositor

Ivanhoe Leung

Article

Correddu et al Sci Rep. 2019 Jun 20;9(1):8884. doi: 10.

Insert

RPS15 (RPS15 Human)

Expression

Bacterial

Promoter

T7

Available Since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pNH-TrxT-G-S10

Plasmid

#127829

Purpose

Production of recombinant human ribosomal protein S10

Depositor

Ivanhoe Leung

Article

Correddu et al Sci Rep. 2019 Jun 20;9(1):8884. doi: 10.

Insert

RPS10 (RPS10 Human)

Tags

His6 - Thioredoxin - TEV

Expression

Bacterial

Promoter

T7

Available Since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pSTAP-seq_fly-4xUAS

Plasmid

#125149

Purpose

Vector to measure the activity of CP candidates in response to a tethered (4xUAS) GAL4-DBD-COF by determining the abundance of transcripts originating from each candidate in fly cells

Depositor

Alexander Stark

Article

Haberle et al Nature. 2019 Jun;570(7759):122-126. doi:

Insert

4 x upstream activating sequence (UAS)

Use

Stap-seq screening vector

Available Since

Aug. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pTEI126

Plasmid

#126066

Purpose

Module 1 vector of PlaMiNGo toolkit for expression of plastid targeted GFP1-10 in plant cell

Depositor

Ralf-Bernd Klösgen

Article

Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs

Insert

Chloroplastic FNR transit peptide

Use

Binary

Tags

GFP1-10

Expression

Plant

Promoter

'long' CaMV35S

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pTEI127

Plasmid

#126067

Purpose

Module 1 vector of PlaMiNGo toolkit for expression of mitochondria targeted GFP1-10 in plant cell

Depositor

Ralf-Bernd Klösgen

Article

Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs

Insert

mitochondria Rieske iron sulphur protein (1-100 aa)

Use

Binary

Tags

GFP1-10

Expression

Plant

Promoter

'long' CaMV35S

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pTEI177

Plasmid

#126077

Purpose

Module 1 vector of PlaMiNGo toolkit for cloning of candidate gene at N-terminus of GFP1-10 and expression in plant cell

Depositor

Ralf-Bernd Klösgen

Article

Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs

Inserts

ccdB Cassette (BsaI)
GFP1-10

Use

Binary, module 1

Tags

linker (GTGGGGS)

Expression

Plant

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pTEI129

Plasmid

#126210

Purpose

Module 1 vector of PlaMiNGo toolkit for cloning of candidate gene at N-terminus of GFP11x7 and expression in plant cell

Depositor

Ralf-Bernd Klösgen

Article

Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs

Inserts

ccdB Cassette (BsaI)
GFP11x7 (5 aa linker)

Use

Binary, module 1

Expression

Plant

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pTEI161

Plasmid

#126211

Purpose

Module 1 vector of PlaMiNGo toolkit for cloning of candidate gene at N-terminus of GFP11 and expression in plant cell

Depositor

Ralf-Bernd Klösgen

Article

Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs

Inserts

ccdB Cassette (BsaI)
GFP11

Use

Binary, module 1

Expression

Plant

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pACYCDuet_W22HDHFR_WTTS

Plasmid

#91241

Purpose

bacterial co-expression of folA (DHFR) and thyA(TS) with a barcode within the noncoding region between the genes

Depositor

Kimberly Reynolds

Article

Schober et al Cell Rep. 2019 Jun 11;27(11):3359-3370.e

Inserts

folA
thyA

Tags

6xHis

Expression

Bacterial

Mutation

W22H

Promoter

T7

Available Since

June 18, 2019

Availability

Academic Institutions and Nonprofits only

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pACYCDuet_F31VDHFR_WTTS

Plasmid

#91227

Purpose

bacterial co-expression of folA (DHFR) and thyA(TS) with a barcode within the noncoding region between the genes

Depositor

Kimberly Reynolds

Article

Schober et al Cell Rep. 2019 Jun 11;27(11):3359-3370.e

Inserts

folA
thyA

Tags

6xHis

Expression

Bacterial

Mutation

F31V

Promoter

T7

Available Since

June 18, 2019

Availability

Academic Institutions and Nonprofits only

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kank1a_pJet1.2

Plasmid

#121978

Purpose

for generating kank1a riboprobes for WISH in zebrafish

Depositor

GuangJun Zhang

Article

Hensley et al Sci Rep. 2016 Jun 13;6:27816. doi: 10.10

Insert

kank1a (si:dkey-146l2.1 Zebrafish)

Use

Cloning vector

Promoter

T7

Available Since

Feb. 28, 2019

Availability

Academic Institutions and Nonprofits only

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SEPT11 sh2 3m shRNA

Plasmid

#122024

Purpose

Negative control shRNA for SEPT11 sh2 shRNA (plasmid 122023).

Depositor

Angel de Blas

Article

Li et al J Biol Chem. 2009 Jun 19;284(25):17253-6

Insert

SEPT11 shRNA (Sept11 Rat)

Use

RNAi

Available Since

Feb. 15, 2019

Availability

Academic Institutions and Nonprofits only

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pMaCTag-21

Plasmid

#120000

Purpose

Template for C-terminal PCR tagging of mammalian genes (without selection) with 24xSunTag

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

24xSunTag

Use

Pcr template

Tags

24xSunTag

Promoter

None

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pMaCTag-Z27

Plasmid

#120070

Purpose

Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with 1xHA

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

1xHA

Use

Pcr template

Tags

1xHA

Promoter

None

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pMaCTag-Z24

Plasmid

#120067

Purpose

Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with TAP

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

TAP

Use

Pcr template

Tags

TAP

Promoter

None

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pMaCTag-Z10

Plasmid

#120053

Purpose

Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with mKOk

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

mKOk

Use

Pcr template

Tags

mKOk

Promoter

None

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pMaCTag-Z01

Plasmid

#120044

Purpose

Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with moxBFP

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

moxBFP

Use

Pcr template

Tags

moxBFP

Promoter

None

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pMaCTag-P16

Plasmid

#120027

Purpose

Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with Dronpa3

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

Dronpa3

Use

Pcr template

Tags

Dronpa3

Promoter

None

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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