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pCDNA5/FRT-ChemoG1

Plasmid

#193806

Purpose

CMV overexpression of ChemoG1 in mammalian cell lines

Depositor

Julien Hiblot

Publication

Hellweg et al Nat Chem Biol. 2023 Jun 8. doi: 10.1038/

Insert

ChemoG1

Tags

10xHis

Expression

Mammalian

Mutation

EGFP: A206K

Promoter

CMV

Available since

June 9, 2023

Availability

Academic Institutions and Nonprofits only

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pYZ714

Plasmid

#132955

Purpose

crRNA entry vector of Pfba1-BsaI pad-TEF1 Term with dFnCas12a-Clr4 in Schizosaccharomyces pombe

Depositor

Jef Boeke

Publication

Zhao et al Nucleic Acids Res. 2020 Jun 4;48(10):578

Insert

dFnCas12a

Use

CRISPR and Synthetic Biology

Tags

SV40 NLS and nucleoplasmin NLS

Promoter

adh1

Available since

Nov. 2, 2022

Availability

Academic Institutions and Nonprofits only

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H2B-2A-GFP-IRESpuro2

Plasmid

#183746

Purpose

Expresses H2B-2A-GFP

Depositor

Daniel Gerlich

Publication

Cuylen et al Nature. 2016 Jun 29;535(7611):308-12. do

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

H2B (H2BC16P Human)

Tags

GFP with 2A protease cleavage site between H2B an…

Expression

Bacterial and Mammalian

Promoter

CMV

Available since

July 27, 2022

Availability

Academic Institutions and Nonprofits only

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pET30-T7-His-sfGFP

Plasmid

#180755

Purpose

pET30 vector missing lacO with sfGFP-S-tag inserted between the Bgl II and T7 terminator

Depositor

Michael Black

Publication

Jew et al Front Bioeng Biotechnol. 2022 Jun 23;10:

Insert

superfolder green fluorescent protein

Tags

strep tag

Expression

Bacterial

Available since

July 19, 2022

Availability

Academic Institutions and Nonprofits only

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SAE1

Plasmid

#175241

Purpose

Bacterial expression and purification, combined with SAE2 to form the E1 enzyme heterodimer

Depositor

Michael Rosen

Publication

Peeples et al Nat Chem Biol. 2021 Jun;17(6):693-702. d

Insert

SAE1 (SAE1 Human)

Expression

Bacterial

Promoter

T7

Available since

Oct. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pJH579

Plasmid

#172163

Purpose

pylBCD variant in pBAD backbone

Depositor

Matthew Bennett

Publication

Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:

Insert

Para (pylBCD JM10.1); AraC

Expression

Bacterial

Mutation

Sumo(S6L) PylB(N61S, E84K, E122K, E175K, E178K, G…

Available since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pJH462

Plasmid

#172154

Purpose

pylBCD variant in pBAD backbone

Depositor

Matthew Bennett

Publication

Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:

Insert

Para (Sumo-tagged pylBCD); AraC

Expression

Bacterial

Mutation

SUMO tag fused to N-terminus of PylB

Available since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pJH577

Plasmid

#172161

Purpose

pylBCD variant in pBAD backbone

Depositor

Matthew Bennett

Publication

Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:

Insert

Para (pylBCD 3f2); AraC

Expression

Bacterial

Mutation

PylB(N61S, E84K, E122K, E178K, G188R, E251D) PylD…

Available since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pJH19

Plasmid

#172141

Purpose

Accessory Plasmid for PACE of pylBCD variants

Depositor

Matthew Bennett

Publication

Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:

Insert

Ppsp gIII.1TAG, luxAB; Plpp pylS; PproK pylT

Expression

Bacterial

Mutation

gIII(P29*)

Available since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG-Bmpr2Δ751-813aa

Plasmid

#163603

Purpose

Expresses BMPR-2 lacking the LIMK-binding region in mammalian cells

Depositor

Takeshi Imai

Publication

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Bmpr2 (Bmpr2 Mouse)

Expression

Mammalian

Mutation

The LIMK-binding region (751-813 aa) is deleted

Available since

Aug. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pHR Fyn-TagBFP

Plasmid

#171035

Purpose

TagBFP-tagged Fyn kinase

Depositor

Jared Toettcher

Publication

Dine et al Cell Rep. 2021 Jun 22;35(12):109280. doi

Insert

Fyn

Use

Lentiviral

Expression

Mammalian

Available since

July 30, 2021

Availability

Academic Institutions and Nonprofits only

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pLKO.1C-shSCR

Plasmid

#139472

Purpose

Replacing the RSV promoter of pLKO.1 with the CMV promoter improves lentiviral production with third-generation viral packaging system.

Depositor

David Cobrinik

Publication

Lee et al Biotechniques. 2020 Jun;68(6):349-352. d

Insert

shSCR

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

CMV

Available since

July 14, 2021

Availability

Academic Institutions and Nonprofits only

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pM116: CasRx VEGFA presgRNA

Plasmid

#166868

Purpose

U6-driven expression of human VEGFA targeting presgRNA compatible with CasRx.

Depositor

Guei-Sheung Liu

Publication

Chuang et al Front Cell Dev Biol. 2021 Jun 11;9:66787

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Human VEGFA targeting presgRNA

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available since

July 1, 2021

Availability

Academic Institutions and Nonprofits only

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pAGM62624

Plasmid

#169094

Purpose

Low copy MoClo level 2 binary vector for plant transformation

Depositor

Sylvestre Marillonnet

Publication

Grutzner et al Front Plant Sci. 2021 Jun 9;12:682443. d

Type

Empty backbone

Use

Moclo cloning vector

Expression

Plant

Available since

June 22, 2021

Availability

Academic Institutions and Nonprofits only

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pAGM49981

Plasmid

#169087

Purpose

MoClo level 0 BvADHα coding sequence module

Depositor

Sylvestre Marillonnet

Publication

Grutzner et al Front Plant Sci. 2021 Jun 9;12:682443. d

Insert

BvADHα

Use

Synthetic Biology; Moclo cloning vector

Available since

June 22, 2021

Availability

Academic Institutions and Nonprofits only

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dGFP-Rabphilin

Plasmid

#170866

Purpose

Mammalian expression of dGFP-Rabphilin.

Depositor

Justin Taraska

Publication

Prasai et al Nat Commun. 2021 Jun 25;12(1):3970. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Rabphilin-3a (RPH3A Bovine)

Tags

dEGFP

Expression

Mammalian

Promoter

CMV

Available since

June 22, 2021

Availability

Academic Institutions and Nonprofits only

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pAGM921

Plasmid

#169086

Purpose

MoClo level 0 Db5GT coding sequence module

Depositor

Sylvestre Marillonnet

Publication

Grutzner et al Front Plant Sci. 2021 Jun 9;12:682443. d

Insert

Db5GT

Use

Synthetic Biology; Moclo cloning vector

Available since

June 22, 2021

Availability

Academic Institutions and Nonprofits only

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pAGM9121_pFMD

Plasmid

#160921

Purpose

Formate dehydrogenase (FMD) promoter module for episomal P. pastoris promoter shuffling

Depositor

Sylvestre Marillonnet

Publication

Pullmann et al ACS Synth Biol. 2021 Jun 2. doi: 10.1021

Insert

Formate dehydrogenase promoter

Use

Synthetic Biology

Mutation

BsaI and BbsI domesticated

Available since

Jan. 28, 2021

Availability

Academic Institutions and Nonprofits only

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pAGM9121_pCAT1

Plasmid

#160918

Purpose

Catalase 1 (CAT1) promoter module for episomal P. pastoris promoter shuffling

Depositor

Sylvestre Marillonnet

Publication

Pullmann et al ACS Synth Biol. 2021 Jun 2. doi: 10.1021

Insert

Catalase 1 promoter

Use

Synthetic Biology

Available since

Jan. 28, 2021

Availability

Academic Institutions and Nonprofits only

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pAG499-FRB-N-frFAST

Plasmid

#160059

Purpose

Expresses FRB-N-frFAST in mammalian cells

Depositor

Arnaud Gautier

Publication

Li et al Angew Chem Int Ed Engl. 2020 Jun 22. doi

Insert

FRB-N-frFAST

Expression

Mammalian

Available since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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