We narrowed to 6,944 results for: SIM;

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OMM-short-CFAST10

Plasmid

#233597

Purpose

Expression of CFAST10 on the OMM membrane after a short linker

Depositor

Riccardo Filadi

Article

Garcia Casas et al Nat Commun. 2024 Nov 12;15(1):9775. doi:

Insert

CFAST10

Expression

Mammalian

Promoter

CMV

Available Since

April 11, 2025

Availability

Academic Institutions and Nonprofits only

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ER-RspA-CFAST

Plasmid

#233607

Purpose

Expression of RspA-CFAST on the ER membrane

Depositor

Riccardo Filadi

Article

Garcia Casas et al Nat Commun. 2024 Nov 12;15(1):9775. doi:

Insert

RspA-CFAST

Expression

Mammalian

Promoter

CMV

Available Since

April 11, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-HA-GTSE1-S91, 262 ,724A

Plasmid

#224737

Purpose

For expression of HA-tagged GTSE1 protein with S91, 262 ,724A mutation

Depositor

Michele Pagano

Article

García-Vázquez et al bioRxiv 2024.06.26.600797

Insert

GTSE1 (GTSE1 Human)

Tags

HA

Expression

Mammalian

Mutation

Serine 91, 262 ,724 to Alanine

Promoter

CMV

Available Since

Dec. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pRetroQ-GTSE1-acGFP

Plasmid

#224740

Purpose

Retroviral vector for delivery and expression of acGFP1-tagged GTSE1 protein

Depositor

Michele Pagano

Article

García-Vázquez et al bioRxiv 2024.06.26.600797

Insert

GTSE1 (GTSE1 Human)

Use

Retroviral

Tags

acGFP

Promoter

CMV

Available Since

Dec. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pAcBac1-Pyl-TAA

Plasmid

#228783

Purpose

Pyl aaRS/tRNA system for mammalian TAA suppression

Depositor

Abhishek Chatterjee

Article

Osgood et al Angew Chem Int Ed Engl. 2023 May 2;62(19

Inserts

PylRS
PytR

Use

Baculoviral

Expression

Mammalian

Mutation

TAA suppressor

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pLIX401 APE1 CI

Plasmid

#227191

Purpose

Inducible lentiviral expression of CI (Catalytically Inactive) APE1

Depositor

David Pellman

Article

Tang et al Nature. 2022 Jun;606(7916):930-936. doi:

Insert

apurinic/apyrimidinic endonuclease APE1 (APEX1 Human)

Use

Lentiviral

Expression

Bacterial and Mammalian

Mutation

H309N

Available Since

Nov. 7, 2024

Availability

Academic Institutions and Nonprofits only

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pU6-hL1-5'_3.3kb plasmid

Plasmid

#226006

Purpose

Plasmid used to generate DNA-FISH probe targeting at human L1HS

Depositor

Miguel Ramalho-Santos

Article

Zhang et al Dev Cell. 2024 Oct 14:S1534-5807(24)0057

Insert

Human L1 element 3.3kb sequences at the 5' end

Use

Synthetic Biology

Available Since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pGL3-Ctr1-gRNA-EGFP

Plasmid

#226000

Purpose

Negative control for CRISPRi-KD

Depositor

Miguel Ramalho-Santos

Article

Zhang et al Dev Cell. 2024 Oct 14:S1534-5807(24)0057

Insert

negtive control sgRNA of no specific targets in human genome

Expression

Mammalian

Available Since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pGL3-Ctr2-gRNA-EGFP

Plasmid

#226001

Purpose

Negative control for CRISPRi-KD

Depositor

Miguel Ramalho-Santos

Article

Zhang et al Dev Cell. 2024 Oct 14:S1534-5807(24)0057

Insert

negtive control sgRNA of no specific targets in human genome

Expression

Mammalian

Available Since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pGL3-NCL-gRNA1-EGFP

Plasmid

#226004

Purpose

CRISPRi-KD of human Nucleolin

Depositor

Miguel Ramalho-Santos

Article

Zhang et al Dev Cell. 2024 Oct 14:S1534-5807(24)0057

Insert

sgRNA targeting human Nucleolin (NCL Human)

Expression

Mammalian

Available Since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pGL3-NCL-gRNA2-EGFP

Plasmid

#226005

Purpose

CRISPRi-KD of human Nucleolin

Depositor

Miguel Ramalho-Santos

Article

Zhang et al Dev Cell. 2024 Oct 14:S1534-5807(24)0057

Insert

sgRNA targeting human Nucleolin (NCL Human)

Expression

Mammalian

Available Since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pET28:LanIV-Chondromyces

Plasmid

#225078

Purpose

Expresses precursor peptide and lanthipeptide synthetase from Chondromyces LanIV (FAST-RiPPs) in E. coli

Depositor

Wilfred van der Donk

Article

Ayikpoe et al Nat Commun. 2022 Oct 17;13(1):6135. doi:

Insert

LanA (LanIV-Chondromyces)

Tags

His6

Expression

Bacterial

Mutation

WT

Promoter

T7

Available Since

Oct. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pET28:LanI-101

Plasmid

#225065

Purpose

Expresses precursor peptide, lanthipeptide cyclase, and lanthipeptide dehydratase from LanI-101 (FAST-RiPPs) in E. coli

Depositor

Wilfred van der Donk

Article

Ayikpoe et al Nat Commun. 2022 Oct 17;13(1):6135. doi:

Insert

LanA (LanI-101)

Tags

His6

Expression

Bacterial

Mutation

WT

Promoter

T7

Available Since

Oct. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pCM0-054

Plasmid

#218204

Purpose

SV40 NLS for protein targeting to nucleus

Depositor

Michael Schroda

Article

Hieronimus et al Microb Cell. 2024 Apr 30;11:128-142. doi

Insert

SV40 NLS

Use

Synthetic Biology

Available Since

Aug. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pAc5.1-PH-mCherry-Oskar 139-240 MUT (A162E/L228E) (HK202)

Plasmid

#206464

Purpose

Oskar 139-240 mut expression in Schneider cells for imaging

Depositor

Mandy Jeske

Article

Salgania et al Nat Commun. 2024 Apr 3;15(1):2875. doi:

Insert

Oskar 139-240 mut (osk Fly)

Tags

PH mcherry

Expression

Insect

Mutation

A162E L228E)

Available Since

July 24, 2024

Availability

Academic Institutions and Nonprofits only

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pSCL475

Plasmid

#219503

Purpose

Express Ade2, Can1 and Faa1 retron msd donor-gRNAs from a single Gal7 promoter, separated by a Csy4 recognition sequence, with an msr expressed in trans

Depositor

Seth Shipman

Article

Gonzalez-Delgado et al Nat Chem Biol. 2024 Nov;20(11):1482-1492

Insert

Ade2, Can1 and Faa1 retron msd donor-gRNAs separated by a Csy4 recognition sequence, with an msr expressed in trans

Expression

Yeast

Promoter

GAL7

Available Since

May 31, 2024

Availability

Academic Institutions and Nonprofits only

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mCherry-POLL

Plasmid

#215141

Purpose

Expresses mCherry-POLL in mammalian cells from a retroviral vector.

Depositor

Michele Pagano

Article

Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

Insert

POLL

Use

Retroviral

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

April 10, 2024

Availability

Academic Institutions and Nonprofits only

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YIp128-CUP1-His6-CUbo(K48R)-GFP

Plasmid

#212793

Purpose

Copper-inducible expression of the artificial test substrate His6-CUbo(K48R)-GFP in budding yeast

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

His6-CUbo(K48R)-GFP

Use

Integrative vector

Expression

Yeast

Mutation

CUb(K48R)

Promoter

CUP1

Available Since

Jan. 29, 2024

Availability

Academic Institutions and Nonprofits only

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YIp128-CUP1-His6-CUbo(K63R)-GFP

Plasmid

#212794

Purpose

Copper-inducible expression of the artificial test substrate His6-CUbo(K63R)-GFP in budding yeast

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

His6-CUbo(K63R)-GFP

Use

Integrative vector

Expression

Yeast

Mutation

CUb(K63R)

Promoter

CUP1

Available Since

Jan. 29, 2024

Availability

Academic Institutions and Nonprofits only

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YIp211-ADH-NUbo-E3(48) aa24-150-VSV

Plasmid

#212747

Purpose

Constitutive expression of NUbo-E3(48)ΔU7BD-VSV in budding yeast, inactive E3(48)

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

E3(48) aa24-150

Use

Integrative vector

Tags

NUbo and VSV

Expression

Yeast

Mutation

Cue1 ΔU7BD

Promoter

ADH1

Available Since

Jan. 24, 2024

Availability

Academic Institutions and Nonprofits only

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