Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 23,191 results for: his

Showing: 10481 - 10520 of 23191 results

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pFR-mTq2-antiFRET-P2A-R-GECO1

Plasmid

#191471

Purpose

Compare the intensity of the genetically encoded calcium sensor (GECI) R-GECO1 to mTurquoise2 fluorescent protein

Depositor

Dorus Gadella

Article

van der Linden et al eLife 14:RP105086; doi: https://doi.org/

Insert

R-GECO1

Tags

His-mTurquoise2-antiFRETlinker-P2A

Expression

Bacterial and Mammalian

Available Since

Dec. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pDRESS-mScarletI-antiFRET-P2A2-linker_noSacI

Plasmid

#191473

Purpose

Negative control for ratiometric comparison between green fluorescent proteins and sensors

Depositor

Dorus Gadella

Article

van der Linden et al eLife 14:RP105086; doi: https://doi.org/

Insert

none

Tags

His-mScarletI-antiFRETlinker-P2A

Expression

Bacterial and Mammalian

Available Since

Nov. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pDRESS-mTq2-antiFRET-P2A2-linker_noSacI

Plasmid

#191472

Purpose

Negative control for ratiometric comparison between red fluorescent proteins and sensors

Depositor

Dorus Gadella

Article

van der Linden et al eLife 14:RP105086; doi: https://doi.org/

Insert

none

Tags

His-mTurquoise2-antiFRETlinker-P2A

Expression

Bacterial and Mammalian

Available Since

Nov. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pFR-mTq2-antiFRET-P2A-CH-GECO2.1

Plasmid

#191470

Purpose

Compare the intensity of the genetically encoded calcium sensor (GECI) CH-GECO2.1 to mTurquoise2 fluorescent protein

Depositor

Dorus Gadella

Article

van der Linden et al eLife 14:RP105086; doi: https://doi.org/

Insert

CH-GECO2.1

Tags

His-mTurquoise2-antiFRETlinker-P2A

Expression

Bacterial and Mammalian

Available Since

Nov. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pFR-mScarletI-antiFRET-P2A-jGCaMP7c

Plasmid

#191461

Purpose

Compare the intensity of the genetically encoded calcium sensor (GECI) jGCaMP7c to mScarlet-I fluorescent protein

Depositor

Dorus Gadella

Article

van der Linden et al eLife 14:RP105086; doi: https://doi.org/

Insert

jGCaMP7c

Tags

His-mScarletI-antiFRETlinker-P2A

Expression

Bacterial and Mammalian

Available Since

Nov. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pET-PLS-KP7_rpoA-U

Plasmid

#190974

Purpose

Test the U-to-C editing activity of KP7 on AtrpoA editing site, in E. coli

Depositor

Bernard Gutmann

Article

Ichinose et al Commun Biol. 2022 Sep 15;5(1):968. doi:

Insert

PLS-KP7

Tags

His

Expression

Bacterial

Promoter

T7

Available Since

Oct. 31, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
PM18033-PLS-KP6-E67A_rpoA-C

Plasmid

#190992

Purpose

Test the C-to-U editing activity of KP6:E67A on AtrpoA editing site, in human cells

Depositor

Bernard Gutmann

Article

Ichinose et al Commun Biol. 2022 Sep 15;5(1):968. doi:

Insert

PLS-KP6

Tags

His

Expression

Mammalian

Mutation

DYW-KP:E67A

Promoter

CMV

Available Since

Oct. 31, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pET-PLS-KP2-E67A_rpoA-U

Plasmid

#190959

Purpose

Test the U-to-C editing activity of KP2:E67A on AtrpoA editing site, in E. coli

Depositor

Bernard Gutmann

Article

Ichinose et al Commun Biol. 2022 Sep 15;5(1):968. doi:

Insert

PLS-KP2

Tags

His

Expression

Bacterial

Mutation

DYW-KP:E67A

Promoter

T7

Available Since

Oct. 31, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pET-PLS-KP6-A66S_rpoA-C

Plasmid

#190968

Purpose

Test the C-to-U editing activity of KP6:A66S on AtrpoA editing site, in E. coli

Depositor

Bernard Gutmann

Article

Ichinose et al Commun Biol. 2022 Sep 15;5(1):968. doi:

Insert

PLS-KP6

Tags

His

Expression

Bacterial

Mutation

DYW-KP:A66S

Promoter

T7

Available Since

Oct. 31, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
PM18033-PLS-KP6-A66S_rpoA-C

Plasmid

#190988

Purpose

Test the C-to-U editing activity of KP6:A66S on AtrpoA editing site, in human cells

Depositor

Bernard Gutmann

Article

Ichinose et al Commun Biol. 2022 Sep 15;5(1):968. doi:

Insert

PLS-KP6

Tags

His

Expression

Mammalian

Mutation

DYW-KP:A66S

Promoter

CMV

Available Since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
PM18033-PLS-KP5_rpoA-U

Plasmid

#190985

Purpose

Test the U-to-C editing activity of KP5 on AtrpoA editing site, in human cells

Depositor

Bernard Gutmann

Article

Ichinose et al Commun Biol. 2022 Sep 15;5(1):968. doi:

Insert

PLS-KP5

Tags

His

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pET-PLS-KP1_rpoA-U

Plasmid

#190955

Purpose

Test the U-to-C editing activity of KP1 on AtrpoA editing site, in E. coli

Depositor

Bernard Gutmann

Article

Ichinose et al Commun Biol. 2022 Sep 15;5(1):968. doi:

Insert

PLS-KP1

Tags

His

Expression

Bacterial

Promoter

T7

Available Since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pET-PLS-KP2-A66S_rpoA-U

Plasmid

#190957

Purpose

Test the U-to-C editing activity of KP2:A66S on AtrpoA editing site, in E. coli

Depositor

Bernard Gutmann

Article

Ichinose et al Commun Biol. 2022 Sep 15;5(1):968. doi:

Insert

PLS-KP2

Tags

His

Expression

Bacterial

Mutation

DYW-KP:A66S

Promoter

T7

Available Since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pET-PLS-KP3-A66S_rpoA-U

Plasmid

#190961

Purpose

Test the U-to-C editing activity of KP3:A66S on AtrpoA editing site, in E. coli

Depositor

Bernard Gutmann

Article

Ichinose et al Commun Biol. 2022 Sep 15;5(1):968. doi:

Insert

PLS-KP3

Tags

His

Expression

Bacterial

Mutation

DYW-KP:A66S

Promoter

T7

Available Since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
PM18033-PLS-KP7_rpoA-U

Plasmid

#190994

Purpose

Test the U-to-C editing activity of KP7 on AtrpoA editing site, in human cells

Depositor

Bernard Gutmann

Article

Ichinose et al Commun Biol. 2022 Sep 15;5(1):968. doi:

Insert

PLS-KP7

Tags

His

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pET-PLS-KP3-E67A_rpoA-U

Plasmid

#190963

Purpose

Test the U-to-C editing activity of KP3:E67A on AtrpoA editing site, in E. coli

Depositor

Bernard Gutmann

Article

Ichinose et al Commun Biol. 2022 Sep 15;5(1):968. doi:

Insert

PLS-KP3

Tags

His

Expression

Bacterial

Mutation

DYW-KP:E67A

Promoter

T7

Available Since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
PM18033-PLS-KP2-E67A_rpoA-U

Plasmid

#190979

Purpose

Test the U-to-C editing activity of KP2:E67A on AtrpoA editing site, in human cells

Depositor

Bernard Gutmann

Article

Ichinose et al Commun Biol. 2022 Sep 15;5(1):968. doi:

Insert

PLS-KP2

Tags

His

Expression

Mammalian

Mutation

DYW-KP:E67A

Promoter

CMV

Available Since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pET-PLS-KP4_rpoA-U

Plasmid

#190964

Purpose

Test the U-to-C editing activity of KP4 on AtrpoA editing site, in E. coli

Depositor

Bernard Gutmann

Article

Ichinose et al Commun Biol. 2022 Sep 15;5(1):968. doi:

Insert

PLS-KP4

Tags

His

Expression

Bacterial

Promoter

T7

Available Since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pET-PLS-KP6-E67A_rpoA-C

Plasmid

#190972

Purpose

Test the C-to-U editing activity of KP6:E67A on AtrpoA editing site, in E. coli

Depositor

Bernard Gutmann

Article

Ichinose et al Commun Biol. 2022 Sep 15;5(1):968. doi:

Insert

PLS-KP6

Tags

His

Expression

Bacterial

Mutation

DYW-KP:E67A

Promoter

T7

Available Since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
PM18033-PLS-KP3-E67A_rpoA-U

Plasmid

#190983

Purpose

Test the U-to-C editing activity of KP3:E67A on AtrpoA editing site, in human cells

Depositor

Bernard Gutmann

Article

Ichinose et al Commun Biol. 2022 Sep 15;5(1):968. doi:

Insert

PLS-KP3

Tags

His

Expression

Mammalian

Mutation

DYW-KP:E67A

Promoter

CMV

Available Since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
PM18033-PLS-KP4_rpoA-U

Plasmid

#190984

Purpose

Test the U-to-C editing activity of KP4 on AtrpoA editing site, in human cells

Depositor

Bernard Gutmann

Article

Ichinose et al Commun Biol. 2022 Sep 15;5(1):968. doi:

Insert

PLS-KP4

Tags

His

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pET-PLS-KP5_rpoA-U

Plasmid

#190965

Purpose

Test the U-to-C editing activity of KP5 on AtrpoA editing site, in E. coli

Depositor

Bernard Gutmann

Article

Ichinose et al Commun Biol. 2022 Sep 15;5(1):968. doi:

Insert

PLS-KP5

Tags

His

Expression

Bacterial

Promoter

T7

Available Since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
PM18033-PLS-KP3-A66S_rpoA-U

Plasmid

#190981

Purpose

Test the U-to-C editing activity of KP3:A66S on AtrpoA editing site, in human cells

Depositor

Bernard Gutmann

Article

Ichinose et al Commun Biol. 2022 Sep 15;5(1):968. doi:

Insert

PLS-KP3

Tags

His

Expression

Mammalian

Mutation

DYW-KP:A66S

Promoter

CMV

Available Since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
PM18033-PLS-KP6-E67A_rpoA-U

Plasmid

#190993

Purpose

Test the U-to-C editing activity of KP6:E67A on AtrpoA editing site, in human cells

Depositor

Bernard Gutmann

Article

Ichinose et al Commun Biol. 2022 Sep 15;5(1):968. doi:

Insert

PLS-KP6

Tags

His

Expression

Mammalian

Mutation

DYW-KP:E67A

Promoter

CMV

Available Since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pETM20-9S-S2 [rpoA]

Plasmid

#176217

Purpose

Expression of TRX-9S-S2 [rpoA]

Depositor

Ian Small

Article

Bernath-Levin et al Synth Biol (Oxf). 2021 Dec 23;7(1):ysab0

Insert

TRX-9S-S2 [rpoA]

Use

Synthetic Biology

Tags

HIS and TRX

Promoter

T7

Available Since

Oct. 24, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pETM20-9P-S2 [rpoA]

Plasmid

#176218

Purpose

Expression of TRX-9P-S2 [rpoA]

Depositor

Ian Small

Article

Bernath-Levin et al Synth Biol (Oxf). 2021 Dec 23;7(1):ysab0

Insert

TRX-9P-S2 [rpoA]

Use

Synthetic Biology

Tags

TRX-His

Promoter

T7

Available Since

Oct. 24, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pETM20-9S-DYW [rpoA]

Plasmid

#176220

Purpose

Expression of TRX-9S-DYW [rpoA]

Depositor

Ian Small

Article

Bernath-Levin et al Synth Biol (Oxf). 2021 Dec 23;7(1):ysab0

Insert

TRX-9S-DYW [rpoA]

Use

Synthetic Biology

Tags

TRX-His

Promoter

T7

Available Since

Oct. 24, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pETM20-avi-9S-S2 [rpoA]

Plasmid

#176223

Purpose

Expression of TRX-AviTag-9S-S2 [rpoA]

Depositor

Ian Small

Article

Bernath-Levin et al Synth Biol (Oxf). 2021 Dec 23;7(1):ysab0

Insert

TRX-Avi-9S-S2 [rpoA]

Use

Synthetic Biology

Tags

TRX-His-AviTag

Promoter

T7

Available Since

Oct. 24, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pBXPHM3-NB-D6

Plasmid

#167989

Purpose

expression of a nanobody against rat PepT2 as a n terminal MBP fusion

Depositor

Simon Newstead

Article

Parker et al Sci Adv. 2021 Aug 25;7(35). pii: 7/35/ea

Insert

NanobodyD6

Tags

His-MBP

Expression

Bacterial

Promoter

pBAD

Available Since

Oct. 7, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pET-15b(+) Pythium oligandrum PRL (Δprenylation motif)

Plasmid

#166423

Purpose

Express pET-15b(+) Pythium oligandrum PRL (Δprenylation motif)

Depositor

Kalle Gehring

Article

Fakih et al J Biol Chem. 2023 Apr;299(4):103055. doi

Insert

Pythium oligandrum PRL

Tags

N-terminal His-tag

Expression

Bacterial

Mutation

Deleted nucleotides coding for 4 C-terminal amino…

Promoter

AmpR, lac1

Available Since

Sept. 15, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pET-15b(+) Reticulomyxa filosa PRL (Δprenylation motif)

Plasmid

#166424

Purpose

Express pET-15b(+) Reticulomyxa filosa PRL (Δprenylation motif)

Depositor

Kalle Gehring

Article

Fakih et al J Biol Chem. 2023 Apr;299(4):103055. doi

Insert

Reticulomyxa filosa PRL

Tags

N-terminal His-tag

Expression

Bacterial

Mutation

Deleted nucleotides coding for 4 C-terminal amino…

Promoter

AmpR, lac1

Available Since

Sept. 15, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pET-15b(+) Sphaeforma arctica PRL (Δprenylation motif)

Plasmid

#166425

Purpose

Express pET-15b(+) Sphaeforma arctica PRL (Δprenylation motif)

Depositor

Kalle Gehring

Article

Fakih et al J Biol Chem. 2023 Apr;299(4):103055. doi

Insert

Sphaeforma arctica PRL

Tags

N-terminal His-tag

Expression

Bacterial

Mutation

Deleted nucleotides coding for 4 C-terminal amino…

Promoter

AmpR, lac1

Available Since

Sept. 15, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pET-15b(+) Acytostelium subglobosum PRL (Δprenylation motif)

Plasmid

#166415

Purpose

Express pET-15b(+) Acytostelium subglobosum PRL (Δprenylation motif)

Depositor

Kalle Gehring

Article

Fakih et al J Biol Chem. 2023 Apr;299(4):103055. doi

Insert

Acytostelium subglobosum PRL

Tags

N-terminal His-tag

Expression

Bacterial

Mutation

Deleted nucleotides coding for 4 C-terminal amino…

Promoter

AmpR, lac1

Available Since

Sept. 15, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pET-15b(+) Caenorhabditis elegans PRL (Δprenylation motif)

Plasmid

#166416

Purpose

Express pET-15b(+) Caenorhabditis elegans PRL (Δprenylation motif)

Depositor

Kalle Gehring

Article

Fakih et al J Biol Chem. 2023 Apr;299(4):103055. doi

Insert

caenorhabditis elegans PRL

Tags

N-terminal His-tag

Expression

Bacterial

Mutation

Deleted nucleotides coding for 4 C-terminal amino…

Promoter

AmpR, lac1

Available Since

Sept. 15, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pET-15b(+) Galdieria sulphuraria PRL (Δprenylation motif)

Plasmid

#166419

Purpose

Express pET-15b(+) Galdieria sulphuraria PRL (Δprenylation motif)

Depositor

Kalle Gehring

Article

Fakih et al J Biol Chem. 2023 Apr;299(4):103055. doi

Insert

Galdieria sulphuraria PRL

Tags

N-terminal His-tag

Expression

Bacterial

Mutation

Deleted nucleotides coding for 4 C-terminal amino…

Promoter

AmpR, lac1

Available Since

Sept. 15, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pET-15b(+) Monosiga brevicollis PRL (Δprenylation motif)

Plasmid

#166420

Purpose

Express pET-15b(+) Monosiga brevicollis PRL (Δprenylation motif)

Depositor

Kalle Gehring

Article

Fakih et al J Biol Chem. 2023 Apr;299(4):103055. doi

Insert

Monosiga brevicollis PRL

Tags

N-terminal His-tag

Expression

Bacterial

Mutation

Deleted nucleotides coding for 4 C-terminal amino…

Promoter

AmpR, lac1

Available Since

Sept. 15, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28a-eGFP-P(11)4

Plasmid

#185786

Purpose

Encodes enhanced green fluorescent protein linked to P(11)4 self-assembling peptide via a GS-linker sequence

Depositor

Lizhong He

Article

Shanbhag et al Adv. Funct. Mater. 2022, 32, 2108039

Insert

eGFP-P(11)4

Tags

His-tag

Expression

Bacterial

Promoter

T7

Available Since

Aug. 10, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28a-ATA-P(11)4

Plasmid

#185787

Purpose

Encodes enzyme ω-aminotransferase linked to P(11)4 self-assembling peptide via a GS-linker sequence

Depositor

Lizhong He

Article

Shanbhag et al Adv. Funct. Mater. 2022, 32, 2108039

Insert

ATA-P(11)4

Tags

His-tag

Expression

Bacterial

Promoter

T7

Available Since

Aug. 8, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28a-GST-P(11)4

Plasmid

#185789

Purpose

Encodes glutathione S-transferase linked to P(11)4 self-assembling peptide via a GS-linker sequence

Depositor

Lizhong He

Article

Shanbhag et al Adv. Funct. Mater. 2022, 32, 2108039

Insert

GST-P(11)4

Tags

His-tag

Expression

Bacterial

Promoter

T7

Available Since

July 22, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pEUK035

Plasmid

#180827

Purpose

T7-inducible expression construct to produce SwGdmB (HY78_09235) in E. coli

Depositor

Gregg Beckham

Article

Bleem et al Chem Catalysis, 2022

Insert

SwGdmB

Tags

6X His

Expression

Bacterial

Promoter

T7

Available Since

June 28, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge

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