We narrowed to 11,787 results for: nsf

1. pAAV-hSyn-fDIO-hM3D(Gq)-mCherry-WPREpA

   Plasmid

   #154868

   Purpose

   Human synapsin-1 promoter; Flp-dependent hM3D(Gq) DREADD-mCherry

   Depositor

   Ulrik Gether

   Has Service

   AAV Retrograde and AAV8

   Insert

   hM3D(Gq)-mCherry
   

   Use

   AAV; Flp-frt

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   Human Synapsin-1

   Available Since

   Aug. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-hSyn-hM4D(Gi)-mCherry

   Plasmid

   #50475

   Purpose

   Gi-coupled hM4D DREADD fused with mCherry under the control of human synapsin promoter.

   Depositor

   Bryan Roth

   Has Service

   AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9

   Insert

   hM4D(Gi)-mCherry
   

   Use

   AAV

   Tags

   mCherry

   Mutation

   See supplemental documents for DREADD mutations

   Promoter

   human Synapsin 1

   Available Since

   April 16, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBiFCt-2in1-CC

   Plasmid

   #105114

   Purpose

   ratiometric BiFC analysis in plants (transient transformation)

   Depositor

   Christopher Grefen

   Article

   Grefen et al Biotechniques. 2012 Oct 2;53(5):311-14.

   Type

   Empty backbone

   Tags

   cYFP-Myc/nYFP-HA

   Expression

   Plant

   Available Since

   Feb. 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-hSyn-hChR2(H134R)-EYFP

   Plasmid

   #26973

   Purpose

   AAV expression of humanized ChR2 with H134R mutation fused to EYFP driven by human Synapsin I promoter for optogenetic excitation

   Depositor

   Karl Deisseroth

   Has Service

   AAV PHP.eB, AAV Retrograde, AAV1, AAV2, AAV5, and AAV9

   Insert

   hChR2(H134R)
   

   Use

   AAV

   Tags

   EYFP

   Expression

   Mammalian

   Mutation

   H134R

   Promoter

   hSyn

   Available Since

   Feb. 4, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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5. pL97

   Plasmid

   #78576

   Purpose

   Over-expression shuttle vector for gene cloning and transfer from E. coli to Streptomyces. Contains strong constitutive ermEp promoter

   Depositor

   Xuming Mao

   Article

   Sun et al Annals of Microbiology Dec 2012, Volume

   Type

   Empty backbone

   Use

   Streptomyces expression

   Expression

   Bacterial

   Promoter

   ermEp* promoter from S. erythraea

   Available Since

   April 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-Dlx5/6-Chronos-eGFP-p2a-nls-mScarlet

   Plasmid

   #121543

   Purpose

   Chronos with red nucleus-targeted fluorescence for inhibitory neurons

   Depositor

   Matthias Prigge

   Insert

   blue-absorbing channelrhodopsin Chronos with eGFP and nls-mScarlet
   

   Use

   AAV

   Promoter

   dlx5/6

   Available Since

   Feb. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pL99

   Plasmid

   #78577

   Purpose

   Over-expression shuttle vector for gene cloning and transfer from E. coli to Streptomyces. Contains inducible PnitA-NitR system

   Depositor

   Xuming Mao

   Article

   Sun et al Annals of Microbiology Dec 2012, Volume

   Type

   Empty backbone

   Use

   Streptomyces expression

   Expression

   Bacterial

   Promoter

   inducible PnitA-NitR system

   Available Since

   May 27, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMPRA1

   Plasmid

   #49349

   Purpose

   Basic backbone for transient transfection of Massively Parallel Reporter Assay (MPRA) libraries

   Depositor

   Tarjei Mikkelsen

   Article

   Melnikov et al J Vis Exp. 2014 Aug 17;(90). doi: 10.379

   Type

   Empty backbone

   Expression

   Mammalian

   Available Since

   Feb. 4, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. 7TFP

   Plasmid

   #24308

   Depositor

   Roel Nusse

   Article

   Fuerer et al PLoS One. 2010 . 5(2):e9370.

   Insert

   7xTcf-FFluc // SV40-PuroR
   

   Use

   Lentiviral

   Available Since

   April 5, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBiFCt-2in1-NC

    Plasmid

    #105112

    Purpose

    ratiometric BiFC analysis in plants (transient transformation)

    Depositor

    Christopher Grefen

    Article

    Grefen et al Biotechniques. 2012 Oct 2;53(5):311-14.

    Type

    Empty backbone

    Tags

    cYFP-Myc and nYFP-HA

    Expression

    Plant

    Available Since

    Feb. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-hSyn-con/fon DREADD Gq-mCherry

    Plasmid

    #200661

    Purpose

    Construct for chemogenetically activating and visualizing subpopulations of neurons in the retina

    Depositor

    Samer Hattar

    Insert

    DREADD Gq-mCherry
    

    Use

    AAV, Cre/Lox, and Mouse Targeting ; Intrsect

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    hSyn

    Available Since

    Nov. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAKgfplux3

    Plasmid

    #199304

    Purpose

    Fluorescence and bioluminescence expression in Gram-negative bacteria

    Depositor

    Attila Karsi

    Article

    Ozdemir et al Pathogens. 2023 May 2;12(5):670. doi: 10

    Insert

    Chloramphenicol acetyltransferase
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV hSyn GFP-Cre

    Plasmid

    #175381

    Purpose

    expresses GFP-Cre under the control of a human synapsin promoter

    Depositor

    Jason Aoto

    Insert

    GFP-Cre recombinase
    

    Use

    AAV

    Tags

    Cre is fused to GFP. GFP is fused to additional n…

    Promoter

    human synapsin

    Available Since

    Nov. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCDH-CB-iCre-P2A-tdTomato-T2A-Puro

    Plasmid

    #72255

    Purpose

    Express iCre, tdTomato and puromycin resistance gene (puromycin N-acetyl-transferase) under CB (chicken beta-actin + CMV enhancer) promoter

    Depositor

    Kazuhiro Oka

    Insert

    iCre-P2A-tdTomato-T2A-Puro
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    CB and chicken beta-actin + CMV enhancer

    Available Since

    March 1, 2017

    Availability

    Academic Institutions and Nonprofits only

15. AAV-CaMKIIa-GCaMP6f-P2A-nls-dTomato

    Plasmid

    #51087

    Purpose

    Forebrain principle neuron specific expression of GCaMP6f Ca sensor and physically separate nuclear localized dTomato fluorophore

    Depositor

    Jonathan Ting

    Insert

    GCaMP6f
    

    Use

    AAV

    Tags

    P2A-nls-dTomato

    Expression

    Mammalian

    Promoter

    CaMKIIa 1.3 kb

    Available Since

    Feb. 28, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLXSN16E7

    Plasmid

    #52396

    Purpose

    The plasmid, pLXSN16E7, is used to produce amphotropic recombinant retrovirus providing efficient gene transfers and expression of the HPV16 E7 gene.

    Depositor

    Denise Galloway

    Article

    Halbert et al J Virol. 1991 Jan;65(1):473-8.

    Insert

    HPV 16E7
    

    Use

    Retroviral

    Available Since

    May 27, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBiFCt-2in1-NN

    Plasmid

    #105111

    Purpose

    ratiometric BiFC analysis in plants (transient transformation)

    Depositor

    Christopher Grefen

    Article

    Grefen et al Biotechniques. 2012 Oct 2;53(5):311-14.

    Type

    Empty backbone

    Tags

    nYFP/cYFP

    Expression

    Plant

    Available Since

    Feb. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-hSyn-con/fon DREADD Gi-mCherry

    Plasmid

    #200660

    Purpose

    Construct for chemogenetically silencing and visualizing subpopulations of neurons in the retina

    Depositor

    Samer Hattar

    Insert

    DREADD Gi-mCherry
    

    Use

    AAV, Cre/Lox, and Mouse Targeting ; Intrsect

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    hSyn

    Available Since

    Nov. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLXSN16E6

    Plasmid

    #52395

    Purpose

    The plasmid, pLXSN16E6, is used to produce amphotropic recombinant retrovirus providing efficient gene transfers and expression of the HPV16 E6 gene.

    Depositor

    Denise Galloway

    Article

    Halbert et al J Virol. 1991 Jan;65(1):473-8.

    Insert

    HPV 16 E6
    

    Use

    Retroviral

    Available Since

    May 27, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. AAV-CaMKIIa-GCaMP6s-P2A-nls-dTomato

    Plasmid

    #51086

    Purpose

    Forebrain principle neuron specific expression of GCaMP6s Ca sensor and physically separate nuclear localized dTomato fluorophore

    Depositor

    Jonathan Ting

    Insert

    GCaMP6s
    

    Use

    AAV

    Tags

    P2A-nls-dTomato

    Expression

    Mammalian

    Promoter

    CaMKIIa 1.3 kb

    Available Since

    March 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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