167,150 results

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pAAV-hsyn-GRAB_ATP1.0

Plasmid

#167577

Purpose

Express the ATP sensor GRAB_ATP1.0 in neurons

Depositor

Yulong Li

Article

Wu et al Neuron. 2021 Dec 17. pii: S0896-6273(21)

Insert

ATP sensor GRAB_ATP1.0 (P2RY1 Human)

Use

AAV

Promoter

hSyn

Available Since

April 13, 2021

Availability

Academic Institutions and Nonprofits only

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SpyTag003-mKate2

Plasmid

#133452

Purpose

Expresses SpyTag003-mKate2 (a far-red fluorescent protein) in bacterial cytoplasm

Depositor

Mark Howarth

Article

Keeble et al Proc Natl Acad Sci U S A. 2019 Dec 10. p

Insert

SpyTag003-mKate2

Tags

GSGSGS linker and His6

Expression

Bacterial

Promoter

T7

Available Since

Dec. 19, 2019

Availability

Academic Institutions and Nonprofits only

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GFP-hPIKfyve

Plasmid

#121148

Purpose

Expresses GFP-tagged human PIKfyve in mammalian cells.

Depositor

Geert van den Bogaart

Article

Baranov et al iScience. 2019 Jan 25;11:160-177. doi: 1

Insert

1-phosphatidylinositol 3-phosphate 5-kinase isoform 2 (PIKFYVE Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

March 8, 2019

Availability

Academic Institutions and Nonprofits only

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VPS13D^mScarlet

Plasmid

#174110

Purpose

The insert is a codon optimized DNA sequence encoding VPS13D with an internal mScarlet protein.

Depositor

Pietro De Camilli

Article

Guillen-Samander et al J Cell Biol. 2021 May 3;220(5). pii: 212

Insert

Codon optimized VPS13D

Tags

mScarlet

Expression

Mammalian

Mutation

With an internal mScarlet tag in aminoacid positi…

Promoter

CMV

Available Since

Dec. 3, 2021

Availability

Academic Institutions and Nonprofits only

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pMK1334

Plasmid

#127965

Purpose

sgRNA expression vector compatible with CROP-Seq and imaging screens

Depositor

Martin Kampmann

Article

Tian et al Neuron. 2019 Aug 5. pii: S0896-6273(19)3

Insert

EF1a-Puro-T2A-2xmycNLS-WPRE-mU6-sgRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

mU6

Available Since

Aug. 24, 2019

Availability

Academic Institutions and Nonprofits only

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CCR7-DuET

Plasmid

#213203

Purpose

The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

Depositor

Thomas Sakmar

Article

Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

Insert

CCR7 (CCR7 Human)

Tags

1D4 and FLAG

Expression

Mammalian

Promoter

CMV, T7

Available Since

April 23, 2024

Availability

Academic Institutions and Nonprofits only

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pHAGE-IDH1-R132C

Plasmid

#116410

Purpose

Lentiviral expression of IDH1 R132C

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

IDH1 (IDH1 Human)

Use

Lentiviral

Mutation

R132C

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pSEVA3b61

Plasmid

#58310

Purpose

Shuttle vector: pUC ori, pTHT15 ori for use in E. coli/B. subtilis. Cannot modify orientation of cat gene; promoter too weak in E. coli, probably transcribed via repB promoter in bicistronic manner.

Depositor

Tom Ellis

Article

Wright et al ACS Synth Biol. 2014 May 13.

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available Since

July 29, 2015

Availability

Academic Institutions and Nonprofits only

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pINDUCER21 (ORF-EG)

Plasmid

#46948

Purpose

Inducible lentiviral gene expression vector. Chloramphenicol cm + ccdB cassette may be removed to clone in gene of interest

Depositor

Stephen Elledge, Thomas Westbrook

Article

Meerbrey et al Proc Natl Acad Sci U S A. 2011 Mar 1;108

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Lentiviral

Available Since

Sept. 26, 2013

Availability

Academic Institutions and Nonprofits only

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pFX-EGFP-Rab7

Plasmid

#174457

Purpose

Express EGFP with late endosome-targeting sequence in mammalian cells

Depositor

Yusuke Ohba

Article

Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

Insert

EGFP with late endosome-targeting sequence

Tags

small GTPase (Rab7)

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR223_JAK2_WT

Plasmid

#81756

Purpose

Gateway Donor vector containing JAK2 , part of the Target Accelerator Plasmid Collection. *Please note this is not a true WT JAK2 sequence and contains a V617F mutation.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

JAK2 (JAK2 Human)

Use

Gateway entry vector

Mutation

V617F

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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SIRT6 Flag

Plasmid

#13817

Purpose

Mammalian expression of human SIRT6 with flag tag

Depositor

Eric Verdin

Article

North et al Mol Cell. 2003 Feb . 11(2):437-44.

Insert

SIRT6 (SIRT6 Human)

Tags

flag

Expression

Mammalian

Available Since

Feb. 19, 2007

Availability

Academic Institutions and Nonprofits only

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pLVX-NPC1(WT)-FLAG

Plasmid

#164972

Purpose

Lentiviral vector for NPC1 expression in mammalian cells

Depositor

Roberto Zoncu

Article

Davis et al Dev Cell. 2020 Dec 7. pii: S1534-5807(20

Insert

NPC1 (NPC1 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Promoter

EF1a

Available Since

Jan. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pBlueSKMimRNA

Plasmid

#102535

Purpose

Template for synthesis of Minos transposase mRNA

Depositor

Michalis Averof

Article

Pavlopoulos et al Genetics. 2004 Jun;167(2):737-46.

Insert

Minos transposase mRNA

Available Since

April 13, 2018

Availability

Academic Institutions and Nonprofits only

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Halo-mitoTag

Plasmid

#240424

Purpose

Visualization of mitochondria

Depositor

Anna Akhmanova

Article

Pasolli et al J Cell Biol. 2025 Sep 1;224(9):e20250400

Insert

actA

Tags

Halotag

Expression

Mammalian

Available Since

Aug. 1, 2025

Availability

Academic Institutions and Nonprofits only

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pZT-AAVS1-R1

Plasmid

#52638

Purpose

TALEN-mediated gene editing at human PPP1R12C/AAVS1 locus

Depositor

Mahendra Rao, Jizhong Zou

Article

Luo et al Stem Cells Transl Med. 2014 May 15. pii:

Insert

Right AAVS1 TALEN

Use

TALEN

Expression

Mammalian

Promoter

CMV

Available Since

May 12, 2014

Availability

Academic Institutions and Nonprofits only

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pBID-UASC

Plasmid

#35200

Depositor

Brian McCabe

Article

Wang et al PLoS One. 2012;7(7):e42102. Epub 2012 Ju

Type

Empty backbone

Use

Drosophila transgene expression

Promoter

Drosophila Synthetic Core Promoter (DSCP)

Available Since

July 26, 2012

Availability

Academic Institutions and Nonprofits only

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GFP-Rab27B

Plasmid

#89447

Purpose

Expression of GFP-tagged human Rab27B in mammalian cells

Depositor

Wendy Westbroek

Article

Hendrix et al J Natl Cancer Inst. 2010 Jun 16;102(12):

Insert

human Rab27B (RAB27B Human)

Tags

eGFP

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pFX-EGFP-Rab5

Plasmid

#174454

Purpose

Express EGFP with early endosome-targeting sequence in mammalian cells

Depositor

Yusuke Ohba

Article

Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

Insert

EGFP with early endosome-targeting sequence

Tags

small GTPase (Rab5)

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pZT-AAVS1-L1

Plasmid

#52637

Purpose

TALEN-mediated gene editing at human PPP1R12C/AAVS1 locus

Depositor

Mahendra Rao, Jizhong Zou

Article

Luo et al Stem Cells Transl Med. 2014 May 15. pii:

Insert

Left AAVS1 TALEN

Use

TALEN

Expression

Mammalian

Promoter

CMV

Available Since

April 28, 2014

Availability

Academic Institutions and Nonprofits only

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