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pJL1-mRFP1

Plasmid

#102630

Purpose

In vitro expression of mRFP1 from the T7 promoter

Depositor

Michael Jewett

Article

Stark et al Sci Adv. 2018 Aug 1;4(8):eaat5107. doi:

Insert

mRFP1

Expression

Bacterial

Promoter

T7

Available Since

Aug. 14, 2018

Availability

Academic Institutions and Nonprofits only

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TRE-UBC-Qa1 SCT-T2A-GFP

Plasmid

#205453

Purpose

lentiviral plasmid for expression of mouse Qa1 SCT

Depositor

Deepta Bhattacharya

Article

Pizzato et al Stem Cell Reports. 2023 Dec 22:S2213-671

Insert

Qa1 SCT (H2-T23 Mouse)

Expression

Mammalian

Promoter

UBC

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pME-Lifeact

Plasmid

#109545

Purpose

Multisite gateway vector for 5' tagging with the actin binding motif Lifeact

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

actin binding motif Lifeact

Use

Zebrafish plasmids

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pAG35

Plasmid

#35123

Depositor

John McCusker

Article

Goldstein et al Yeast. 1999 Oct;15(14):1541-53.

Insert

natMX3

Use

Yeast replicating

Available Since

Sept. 27, 2012

Availability

Academic Institutions and Nonprofits only

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HDR-HaloPOLQ

Plasmid

#211637

Purpose

halo donor

Depositor

Agnel Sfeir

Article

Brambati et al Science. 2023 Aug 11;381(6658):653-660.

Insert

Puro-LoxHaloTag

Tags

flag

Expression

Mammalian

Promoter

SV40

Available Since

Jan. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pATetO 6XHis T5 Exonuclease

Plasmid

#206209

Purpose

Inducible expression of T5 exonuclease

Depositor

Andrew Ellington

Article

Bhadra et al PLoS One. 2018 Aug 15;13(8):e0201681. do

Insert

T5 exonuclease

Expression

Bacterial

Available Since

Dec. 14, 2023

Availability

Academic Institutions and Nonprofits only

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L2_lacZgRNA-Cas9-CsA

Plasmid

#136138

Purpose

Plasmid to accept gRNA target with SapI, lacZ blue-white screening. Contains Cas9 and HygR.

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

p5-35Sx2:HygR p5-MpU6:lacZgRNA p5-MpEF1a:Cas9-NLS

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available Since

Nov. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti CMV GFP Zeo (637-7)

Plasmid

#17449

Purpose

3rd gen lentiviral eGFP expression vector, CMV promoter, Zeo

Depositor

Eric Campeau, Paul Kaufman

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Insert

GFP

Use

Lentiviral

Expression

Mammalian

Available Since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

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pT7-VP2SA11

Plasmid

#89163

Purpose

Express Rotavirus SA11 VP2 in mammalian cells

Depositor

Takeshi Kobayashi

Article

Kanai et al Proc Natl Acad Sci U S A. 2017 Jan 30. p

Insert

Rotavirus SA11 VP2

Expression

Mammalian

Promoter

T7 promotor

Available Since

April 10, 2017

Availability

Academic Institutions and Nonprofits only

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pFA6a-mNeonGreen-HIS3MX6

Plasmid

#129100

Purpose

For tagging genes at 3'end (C-terminal end of protein) in S. cerevisiae with mNeonGreen

Depositor

Wei-Lih Lee

Insert

mNeonGreen

Expression

Yeast

Available Since

July 31, 2019

Availability

Academic Institutions and Nonprofits only

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pNEU-hMbPylRS-4xU6M15

Plasmid

#105830

Purpose

Plasmid encoding for the Pyrrolysine translational pair that enables efficent incoporation of Pyl-like click amino acids in response to the amber codon into proteins in mammalian cells

Depositor

Irene Coin

Article

Serfling et al Nucleic Acids Res. 2017 Nov 21. pii: 464

Inserts

Humanized MbPylRS
tRNAM15

Expression

Mammalian

Mutation

(Y271A, Y349F)MbPylRS

Promoter

CMV and U6

Available Since

March 15, 2018

Availability

Academic Institutions and Nonprofits only

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R777-E229 Hs.RASA2

Plasmid

#70513

Purpose

Gateway ORF clone of human RASA2 [NM_006506.3] with stop codon (for native or N-terminal fusions)

Depositor

Dominic Esposito

Insert

RASA2 (RASA2 Human)

Use

Gateway Cloning

Available Since

March 1, 2016

Availability

Academic Institutions and Nonprofits only

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pET22b RNase A

Plasmid

#58903

Purpose

Bacterial expression of bovine pancreatic ribonuclease A

Depositor

Ronald Raines

Article

delCardayre et al Protein Eng. 1995 Mar;8(3):261-73.

Insert

RNase A (RNASE1 Bovine)

Tags

pelB sequence

Expression

Bacterial

Mutation

See Depositor Comments below

Promoter

T7

Available Since

Feb. 12, 2015

Availability

Academic Institutions and Nonprofits only

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pYPQAT56

Plasmid

#223428

Purpose

T-DNA vector for dSpCas9 mediated gene activation for dicot plants; NGG PAM; dSpCas9-Act3.0 was driven by ZmUbi1 and the sgRNA was driven by AtU3 promoter; BASTA for plants selection.

Depositor

Yiping Qi

Article

Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

Insert

ZmUbi-dSpCas9-Act3.0-AtU3-gRNA scaffold 2.0

Use

CRISPR

Tags

3x FLAG and NLS

Expression

Plant

Available Since

April 10, 2025

Availability

Academic Institutions and Nonprofits only

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FAB(PX-LD)

Plasmid

#214426

Purpose

Detection of peroxisome-lipid droplet contact site

Depositor

Chi-Lun Chang

Article

Li et al J Cell Biol. 2024 Sep 2;223(9):e20231112

Insert

FKBP-V5

Tags

CFAST-Halo and FKBP-V5-RspAN

Expression

Mammalian

Promoter

CMV

Available Since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-GINKO1

Plasmid

#113112

Purpose

Mammalian expression of genetically encoded green fluorescent potassium ion indicator GINKO1

Depositor

Robert Campbell

Article

Shen et al Commun Biol. 2019 Jan 14;2:18. doi: 10.1

Insert

GINKO1

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 23, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV-FLEx-TetOn3G-3xHA-FLEx

Plasmid

#133729

Purpose

Plasmid contains the floxed TetOn3G-transactivator sequence for expression of TetOn3G in the presence of Cre recombinase.

Depositor

Erin Schuman

Article

Heumuller et al Nat Methods. 2019 Jul 15. pii: 10.1038/s

Insert

TetOn3G-3xHA (floxed & inverted)

Use

AAV

Tags

3xHA-tag

Expression

Mammalian

Promoter

pCMV

Available Since

Dec. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pLKO.1 3xHA-KillerRed-OMP25

Plasmid

#174544

Purpose

localizes KillerRed to mitochondria

Depositor

Minna Roh-Johnson

Article

Kidwell et al eLife. 2023 Mar 6;12:e85494. doi: 10.755

Insert

3xHA-KillerRed-OMP25

Use

Lentiviral

Expression

Mammalian

Available Since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pTagBFP-C1-FKBP-PIP5K1C-HD-EE

Plasmid

#220090

Purpose

Recruitable PIP5K to make PIP2

Depositor

Gerry Hammond

Article

Weckerly et al J Cell Biol. 2025 Nov 3;224(11):e2024051

Insert

PIP5K1C (PIP5K1C Human)

Tags

TagBFP-FKBP1A(3-108)

Expression

Mammalian

Mutation

amino acids 1-635; D101R, R304D, R445E, K446E

Promoter

CMV

Available Since

May 21, 2024

Availability

Academic Institutions and Nonprofits only

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pEN396 - pCAGGS-Tir1-V5-2A-PuroR TIGRE donor

Plasmid

#92142

Purpose

Targeting vector to introduce an osTir1 expressing cassette at the mouse TIGRE acceptor locus using Puromycin selection (2A-fusion). Auxin-inducible degron system.

Depositor

Benoit Bruneau

Article

Nora et al Cell. 2017 May 18;169(5):930-944.e22. do

Insert

osTir1

Use

Mouse Targeting

Tags

V5 tag

Expression

Mammalian

Available Since

June 26, 2017

Availability

Academic Institutions and Nonprofits only

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