161,805 results

1. FUCas9Cherry

   Plasmid

   #70182

   Purpose

   Expresses Cas9 with fluorescent Cherry reporter

   Depositor

   Marco Herold

   Article

   Aubrey et al Cell Rep. 2015 Mar 3;10(8):1422-32. doi:

   Insert

   humanised S.pyogenes cas9
   

   Use

   CRISPR and Lentiviral

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   hUbC

   Available since

   Nov. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. TetO-Ascl1-puro

   Plasmid

   #97329

   Purpose

   Generating lentiviruses to express Ascl1 and puromycin resistance

   Depositor

   Marius Wernig

   Article

   Yang et al Nat Methods. 2017 May 15. doi: 10.1038/n

   Insert

   Ascl1 (Ascl1 Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   TET-ON

   Available since

   July 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBJ FLAG-hPar1

   Plasmid

   #53226

   Purpose

   mammalian expression of human Par1 with N-terminal FLAG tag

   Depositor

   Shaun Coughlin

   Article

   Ishii et al J Biol Chem. 1993 May 5. 268(13):9780-6.

   Insert

   Par1 (F2R Human)
   

   Use

   Tags

   FLAG and bovine prolactin signal sequence (MDSKGS…

   Expression

   Mammalian

   Mutation

   Deletion of aa1-39 (native signal peptide)

   Promoter

   SV40

   Available since

   Aug. 6, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pACYC-Skp-GroEL/ES

   Plasmid

   #83922

   Purpose

   Co-expresses cytoplasmic copies of the Skp and Gro EL/ES protein folding chaperones.

   Depositor

   Karl Griswold

   Article

   Lamppa et al J Biotechnol. 2013 Mar 10;164(1):1-8. do

   Inserts

   Skp
   Gro EL/ES
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Nov. 28, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHAGE2-EF1a-FOATPMLS-2xFLAG-T4Lig-IRES-Neo

   Plasmid

   #234954

   Purpose

   Human codon optimized T4 DNA Ligase with N-terminal F0ATP MLS expressing plasmid

   Depositor

   Agnel Sfeir

   Article

   Fu et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

   Insert

   human codon optimized T4 DNA Ligase with N-terminal mitochondrial localization signal of subunit 9 of F0-ATP synthase
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   April 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHAGE2-EF1a-E1MLS-2xFLAG-LigD-IRES-tdTomato

   Plasmid

   #234951

   Purpose

   Human codon optimized LigD (Mycobacterium) with N-terminal E1 MLS expressing plasmid

   Depositor

   Agnel Sfeir

   Article

   Fu et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

   Insert

   Human codon optimized LigD with N-terminal mitochondrial localization signal of pyruvate dehydrogenase E1 subunit
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   EF1A

   Available since

   April 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pPtPuc3

   Plasmid

   #62863

   Purpose

   Episome vector for Phaeodactylum tricornutum (pUC-based)

   Depositor

   Hamilton Smith

   Article

   Karas et al Nat Commun. 2015 Apr 21;6:6925. doi: 10.

   Type

   Empty backbone

   Use

   Synthetic Biology; Episomal vector for phaeodacty…

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 13, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. AiP11402-pAAV-AiE0058h-minBG-SYFP2-WPRE3-BGHpA (Alias: CN1402)

   Plasmid

   #163494

   Purpose

   AiE0058h is an enhancer sequence designed to drive AAV-mediated transgene expression in L4/5_IT cortical neurons

   Depositor

   Jonathan Ting, The Allen Institute for Brain Science

   Article

   Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

   Insert

   SYFP2
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   minBetaGlobin

   Available since

   Feb. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. AAVS1-TRE3G-EGFP

   Plasmid

   #52343

   Purpose

   AAVS1 donor vector for inducible gene expression

   Depositor

   Su-Chun Zhang

   Article

   Qian et al Stem Cells. 2014 Feb 4. doi: 10.1002/ste

   Insert

   EGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TRE3G

   Available since

   Nov. 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-hSynapsin1-axon-GCaMP6s-P2A-mRuby3

    Plasmid

    #112005

    Purpose

    For enriched expression of GCaMP6s in axons with cytosolic expression of mRuby3

    Depositor

    Lin Tian

    Article

    Broussard et al Nat Neurosci. 2018 Sep;21(9):1272-1280.

    Has Service

    AAV5 and AAV9

    Insert

    axon-GCaMP6s-P2A-mRuby3
    

    Use

    AAV

    Tags

    GAP43 palmitoylation domain

    Expression

    Mammalian

    Mutation

    Promoter

    hSynapsin1

    Available since

    June 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. Myosin-IIA-GFP

    Plasmid

    #38297

    Depositor

    Matthew Krummel

    Article

    Jacobelli et al J Immunol. 2009 Feb 15;182(4):2041-50.

    Insert

    Myosin II A (Myh9 Mouse)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 21, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLenti_EF1a_Flag_EGFP

    Plasmid

    #183117

    Purpose

    Expresses flag-tagged EGFP in mammalian cells

    Depositor

    William Kaelin

    Article

    Stransky et al Proc Natl Acad Sci U S A. 2022 Apr 5;119

    Insert

    EGFP
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Aug. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLminP_Luc2P_RE23

    Plasmid

    #90365

    Purpose

    ATF4 - Amino acid deprivation gene reporter

    Depositor

    Ramnik Xavier

    Article

    O'Connell et al Cell Syst. 2016 May 25;2(5):323-34. doi:

    Insert

    Firefly luciferase
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    AARE

    Available since

    Aug. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMRXIB-mRuby3-LC3

    Plasmid

    #221738

    Purpose

    Stable expression of mRuby3-LC3 in mammalian cells

    Depositor

    Noboru Mizushima

    Article

    Shinoda et al Elife. 2024 Jun 4;12:RP92189. doi: 10.75

    Insert

    LC3B (Map1lc3b Rat)
    

    Use

    Retroviral

    Tags

    mRuby3

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. 4xCSL-luciferase

    Plasmid

    #41726

    Depositor

    Raphael Kopan

    Article

    Saxena et al J Biol Chem. 2001 Oct 26;276(43):40268-7

    Insert

    4xCSL binding sites
    

    Use

    Luciferase

    Tags

    Firefly luciferase

    Expression

    Mammalian

    Mutation

    Contains a multimerized high affinity CSL binding…

    Promoter

    Available since

    Dec. 13, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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16. mCherry-Kinesin11-N-18

    Plasmid

    #55067

    Purpose

    Localization: Motor Protein/Microtubule Filaments, Excitation: 587, Emission: 610

    Depositor

    Michael Davidson

    Insert

    Kinesin11 (KIF11 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-hSYN-miRFP670nano3-WPRE-hGHpA

    Plasmid

    #184688

    Purpose

    Mammalian expression, miRFP670nano3

    Depositor

    Vladislav Verkhusha, Axel Nimmerjahn

    Article

    Oliinyk et al Nat Methods. 2022 May 23. pii: 10.1038/s

    Insert

    miRFP670nano3
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    hSYN

    Available since

    June 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. LentiGuide-Neo

    Plasmid

    #139449

    Purpose

    Lentiviral vector with gRNA scaffold and neomycin selectable marker

    Depositor

    Caroline Goujon

    Article

    Doyle et al Nat Microbiol. 2018 Dec;3(12):1369-1376.

    Insert

    no sgRNA inserted; resistance gene: neoR
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a / U6

    Available since

    Aug. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCB-SC101-Spe

    Plasmid

    #133765

    Purpose

    shuttle vector backbone

    Depositor

    Himadri Pakrasi

    Article

    Liu et al Microb Cell Fact. 2018 Mar 26;17(1):48.

    Type

    Empty backbone

    Use

    Synthetic Biology; Shuttle vector for e.coli and …

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pNIR-FbmCherry

    Plasmid

    #184676

    Purpose

    NIR-Fb to mCherry

    Depositor

    Vladislav Verkhusha

    Article

    Oliinyk et al Nat Methods. 2022 May 23. pii: 10.1038/s

    Insert

    NIR-FbmCherry
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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