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pDONR223_EGFR_p.T790M_p.L858R

Plasmid

#82914

Purpose

Gateway Donor vector containing EGFR, part of the Target Accelerator Plasmid Collection.

Depositor

David Root, Matthew Meyerson, Jesse Boehm

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

EGFR (EGFR Human)

Use

Gateway entry vector

Tags

Expression

Mutation

T790M, L858R

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pX458-Ef1a-Cas9-H2B-mCherry

Plasmid

#159655

Purpose

Empty backbone for cloning gRNA sequences for DNA targeting. Contains an T2A linked H2B mCherry nuclear reporter.

Depositor

Joseph Brzezinski

Article

Kaufman et al Development. 2021 Jun 15;148(12). pii: 2

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

March 19, 2021

Availability

Academic Institutions and Nonprofits only

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EGFP-Talin head-SpyCatcher003

Plasmid

#133566

Purpose

Expresses EGFP-Talin head-SpyCatcher003 in mammalian cells

Depositor

Mark Howarth

Article

Keeble et al Proc Natl Acad Sci U S A. 2019 Dec 10. p

Insert

EGFP-Talin head-SpyCatcher003

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pShuttle-CMV-iRFP

Plasmid

#31856

Purpose

a phytochrome-based near-infrared fluorescent protein, also known as iRFP713, used for in vivo imaging

Depositor

Vladislav Verkhusha

Article

Filonov et al Nat Biotechnol. 2011 Jul 17. doi: 10.103

Insert

iRFP

Use

Adenoviral

Tags

Expression

Mammalian

Mutation

T2A, S13L, A92T, V104I, V114I, E161K, Y193K, F198…

Promoter

CMV

Available since

Sept. 6, 2011

Availability

Academic Institutions and Nonprofits only

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pCMV/SB11

Plasmid

#26552

Depositor

Perry Hackett

Article

Ivics et al Cell. 1997 Nov 14. 91(4):501-10.

Insert

Sleeping Beauty

Use

Tags

Expression

Bacterial

Mutation

Methionine 243 to Glutamine Threonine 136 to Arg…

Promoter

Available since

Oct. 25, 2010

Availability

Academic Institutions and Nonprofits only

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CL20_mEGFP_PAX3_FOXO1

Plasmid

#205875

Purpose

Express mEGFP-tagged fusion protein, PAX3_FOXO1 from patient-derived sequence

Depositor

Richard Kriwacki

Article

Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

Insert

PAX3_FOXO1 (PAX3, FOXO1 Human)

Use

Tags

mEGFP

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

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xCas9 3.7

Plasmid

#108379

Purpose

Mammalian expression vector for xCas9 3.7

Depositor

David Liu

Article

Hu et al Nature. 2018 Feb 28. pii: nature26155. d

Insert

xCas9 3.7

Use

Tags

NLS

Expression

Mammalian

Mutation

A262T, R324L, S409I, E480K, E543D, M694I, E1219V

Promoter

Available since

April 17, 2018

Availability

Academic Institutions and Nonprofits only

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pPV-EF1a-EGxxFP(DMDex45_10)-iP-A

Plasmid

#204621

Purpose

Single-strand annealing (SSA) vector for monitoring crRNA(DMDex45)'s activity. DMD intron 44 region (upstream of exon 45)

Depositor

Akitsu Hotta

Article

Kita et al Stem Cell Reports. 2023 Sep 12;18(9):175

Insert

EGFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

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Crop-Seq-BFP-WPRE-TS-hU6-BsmbI

Plasmid

#137993

Purpose

Backbone for generation of ClonMapper gRNA barcode library for use in droplet-based sequencing

Depositor

Amy Brock

Article

Gardner et al Methods Mol Biol. 2022;2394:109-131. doi

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pTK5

Plasmid

#37391

Depositor

Iain Cheeseman

Article

Kiyomitsu et al Nat Cell Biol. 2012 Feb 12;14(3):311-7.

Insert

ACTR1A (ACTR1A Human)

Use

Tags

S-tag, TEV, and mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 19, 2012

Availability

Academic Institutions and Nonprofits only

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YIP sHRPa-Aga1P

Plasmid

#73151

Purpose

Large fragment of split HRP fused to the N-terminus of the yeast mating protein Aga1P. When linearized, this vector incorporates into the yeast genome.

Depositor

Alice Ting

Article

Martell et al Nat Biotechnol. 2016 May 30. doi: 10.103

Insert

sHRPa-Aga1P

Use

Tags

V5 tag

Expression

Yeast

Mutation

Promoter

glyceraldehyde-3-phosphate dehydrogenase GPD prom…

Available since

June 15, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV-FLEX-SaCas9-U6-sgGria1

Plasmid

#124853

Purpose

Mutagenesis of Gria1

Depositor

Larry Zweifel

Article

Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

Insert

Gria1 (Gria1 Mouse)

Use

AAV, CRISPR, and Mouse Targeting

Tags

Expression

Mutation

Promoter

Available since

May 6, 2019

Availability

Academic Institutions and Nonprofits only

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pCHW110035

Plasmid

#222444

Purpose

Level 0, N-terminal tag module (CCAT-AATG) containing LoxP-mCherry-stop.

Depositor

Chih-Hang Wu

Article

Chiang et al bioRxiv 2024.03.23.586378

Insert

LoxP-mCherry-stop

Use

Synthetic Biology

Tags

Expression

Plant

Mutation

no

Promoter

Available since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR223_NRAS_WT

Plasmid

#82151

Purpose

Gateway Donor vector containing NRAS , part of the Target Accelerator Plasmid Collection.

Depositor

William Hahn, David Root, Jesse Boehm

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

NRAS (NRAS Human)

Use

Gateway entry vector

Tags

Expression

Mutation

WT

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223-CAMK2D

Plasmid

#23814

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

CAMK2D (CAMK2D Human)

Use

Gateway donor vector

Tags

Expression

Mutation

Promoter

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pPD95_75

Plasmid

#1494

Depositor

Andrew Fire

Type

Empty backbone

Use

Tags

IVSA, gfpN, IVSR, gfpS65C, IVSS, IVST, gfpC, IVSL…

Expression

Worm

Mutation

Promoter

Available since

March 7, 2005

Availability

Academic Institutions and Nonprofits only

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pZac2.1-GfaABC1D-mCherry-hPMCA2w/b

Plasmid

#111568

Purpose

Expresses a plasma membrane calcium pump hPMCA2w/b

Depositor

Baljit Khakh

Article

Yu et al Neuron. 2018 Aug 27. pii: S0896-6273(18)

Has Service

AAV5

Insert

Plasma membrane calcium pump 2, variant w/b (ATP2B2 Human)

Use

AAV

Tags

mCherry

Expression

Mutation

Promoter

Available since

Sept. 25, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti_EF1a_Flag_TP53_R248W

Plasmid

#183114

Purpose

Expresses flag-tagged p53 with R248W mutation in mammalian cells

Depositor

William Kaelin

Article

Stransky et al Proc Natl Acad Sci U S A. 2022 Apr 5;119

Insert

TP53 (TP53 Human)

Use

CRISPR and Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

changed arginine 248 to tryptophan

Promoter

EF1a

Available since

April 25, 2023

Availability

Academic Institutions and Nonprofits only

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pN565

Plasmid

#49990

Purpose

expresses an attenuated T7 RNAP under an isopropyl β-d-1-thiogalactopyranoside (IPTG) inducible control

Depositor

Christopher Voigt

Article

Rhodius et al Mol Syst Biol. 2013 Oct 29;9:702. doi: 1

Insert

T7 RNAP*

Use

Synthetic Biology

Tags

umuD degradation tag

Expression

Bacterial

Mutation

R632S

Promoter

pTac-symmetric

Available since

Jan. 31, 2014

Availability

Academic Institutions and Nonprofits only

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pFAST-BAC BAF155/SMARCC1-His

Plasmid

#177863

Purpose

Transfer vector to generate recombinant baculovirus expressing BAF155/SMARCC1-His

Depositor

Yoshihiro Izumiya

Article

Shimoda et al Commun Biol. 2021 Dec 2;4(1):1330. doi:

Insert

SMARCC1 (SMARCC1 Human)

Use

Tags

His and His tag at C-terminus with GGGGS linker

Expression

Insect

Mutation

Promoter

polyhedrin

Available since

Dec. 3, 2021

Availability

Academic Institutions and Nonprofits only

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