We narrowed to 11,734 results for: Hal

1. pSB700 lenti gRNA Puro_zhang2.0

   Plasmid

   #167919

   Purpose

   Lentiviral vector for expressing U6 MS2-sgRNA cloning plasmid

   Depositor

   Nan Cher Yeo, Alejandro Chavez

   Article

   Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pV1F

   Plasmid

   #205084

   Purpose

   Prokaryotic expressing of AtVIP1 fused with a sequence encoding a T4SS translocation tag in A. tumefaciens.

   Depositor

   Suomeng Dong

   Article

   Wang et al PLoS Pathog. 2023 Apr 21;19(4):e1011346.

   Insert

   AtVIP1
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   agorbacterium virB operon promoter

   Available since

   Aug. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. NAX_CAGGS_CAS9_Puro

   Plasmid

   #167863

   Purpose

   PiggyBac compatible plasmid expressing spCas9

   Depositor

   Nan Cher Yeo, Alejandro Chavez

   Article

   Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

   Insert

   Cas9
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pcDNA3.1/Puro-CAG-VSFP-CR

   Plasmid

   #40257

   Depositor

   Michael Lin

   Article

   Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

   Insert

   VSFP-CR
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Oct. 18, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. PE_CO

   Plasmid

   #190335

   Purpose

   Prime editor (PE2) with codon-optimized Reverse transcriptase for prime editing in mammalian cells

   Depositor

   Rasmus Bak

   Article

   Gao et al Mol Ther. 2022 Jul 8. pii: S1525-0016(22

   Insert

   PE2 with codon-optimized reverse transcriptase
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pLVX-IRES Hyg SMAD4

   Plasmid

   #107128

   Purpose

   Constitutive Flag-tagged SMAD4 expression in Mammalian cells

   Depositor

   Joan Massague

   Article

   David et al Cell. 2016 Feb 25;164(5):1015-30. doi: 1

   Insert

   SMAD4 (SMAD4 Human)
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mutation

   Promoter

   Available since

   Feb. 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. hRIP1 HA GFP K45A

   Plasmid

   #41389

   Depositor

   Francis Chan

   Article

   Cho et al Cell. 2009 Jun 12;137(6):1112-23.

   Insert

   RIP1 (RIPK1 Human)
   

   Use

   Tags

   GFP and HA

   Expression

   Mammalian

   Mutation

   Kinase Domain Mutant (K45A)

   Promoter

   CMV

   Available since

   Dec. 3, 2012

   Availability

   Academic Institutions and Nonprofits only

8. EGFP-P2A-APEX2-csChBP

   Plasmid

   #108877

   Purpose

   Expression of APEX2 fused to a conditionally stable nanobody against mCherry (includes green cytoplasmic reporter)

   Depositor

   Rob Parton

   Article

   Ariotti et al PLoS Biol. 2018 Apr 5;16(4):e2005473. do

   Insert

   EGFP-P2A-APEX2-csChBP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pSL2620 (pTniQ_Vch)

   Plasmid

   #200892

   Purpose

   Expresses human codon-optimized V. cholerae TniQ from a CMV promoter.

   Depositor

   Samuel H. Sternberg

   Article

   Lampe et al Nat Biotechnol. 2023 Mar 29. doi: 10.103

   Insert

   VchTniQ
   

   Use

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. Fragment 1 Null

    Plasmid

    #198729

    Purpose

    Gateway-compatible gRNA backbone . Template for the addition of target sequences to produce 2x gRNA.

    Depositor

    Friedrich Kragler

    Article

    Yang et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    gRNA expression cassette in pENTR4
    

    Use

    Gateway-compatible entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pAct:dCas9

    Plasmid

    #78903

    Purpose

    Expresses dCas9 under actin promoter for "Scaffold" CRISPRa in Drosophila cells

    Depositor

    Norbert Perrimon

    Article

    Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

    Insert

    dCas9
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    pActin (Drosophila)

    Available since

    Aug. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. U6c

    Plasmid

    #117223

    Purpose

    Expresses gRNA in Aedes aegypti

    Depositor

    Omar Akbari

    Article

    Li et al Elife. 2020 Jan 21;9. pii: 51701. doi: 1

    Insert

    HR U6c promoter-gRNA
    

    Use

    Synthetic Biology

    Tags

    Expression

    Insect

    Mutation

    Promoter

    U6

    Available since

    Jan. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pMOD_C'5802

    Plasmid

    #176879

    Purpose

    High constitutive expression of Firefly Luciferase in plants

    Depositor

    Daniel Voytas

    Article

    Cody et al Nat Protoc. 2022 Oct 17. pii: 10.1038/s4

    Insert

    AtUbi10:Luc+:RbcsE9
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. U6b

    Plasmid

    #117222

    Purpose

    Expresses gRNA in Aedes aegypti

    Depositor

    Omar Akbari

    Article

    Li et al Elife. 2020 Jan 21;9. pii: 51701. doi: 1

    Insert

    HR U6b promoter-gRNA
    

    Use

    Synthetic Biology

    Tags

    Expression

    Insect

    Mutation

    Promoter

    U6

    Available since

    Jan. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pVP2A-PIF6

    Plasmid

    #73369

    Purpose

    Expresses Adeno-associated virus VP2 with PIF6 insert.

    Depositor

    Junghae Suh

    Article

    Gomez et al ACS Nano. 2015 Nov 30.

    Insert

    PIF6 (PIL2 Mustard Weed)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. MTK Lentivirus Single Cassette Destination Vector (pAN2414)

    Plasmid

    #194220

    Purpose

    Backbone for constructing single casette expression plasmids using the Mammalian Tookit

    Depositor

    Hana El-Samad

    Article

    Weinberg et al bioRxiv 2021.04.20.440678

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. mOtop2_pGEMHE

    Plasmid

    #114675

    Purpose

    This construct was used to express mouse Otop2 in Xenopus laevis oocytes

    Depositor

    Emily Liman

    Article

    Tu et al Science. 2018 Mar 2;359(6379):1047-1050.

    Insert

    Otopetrin 2 (Otop2 Mouse)
    

    Use

    For generating the mrna, which can highly express…

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    July 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pHR_PGK_SNIPR_ADAM17 site

    Plasmid

    #188349

    Purpose

    Lentiviral vector - constitutive expression of an anti-CD19 SNIPR with a ADAM17site, a Notch1-TMD, a Notch1-JMD, and a Gal4VP64 transcriptional factor

    Depositor

    Kole Roybal

    Article

    Zhu et al Cell. 2022 Apr 14;185(8):1431-1443.e16.

    Insert

    PGK_antiCD19_ADAM17site_Notch1-TMD_Notch1-JMD_Gal4VP64
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. NAX_CAGGS_CAS9_m4_Blasto

    Plasmid

    #167864

    Purpose

    PiggyBac compatible plasmid expressing dCas9

    Depositor

    Nan Cher Yeo, Alejandro Chavez

    Article

    Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

    Insert

    dCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    D10A, D839A, H840A, N863A

    Promoter

    Available since

    Feb. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pNK869

    Plasmid

    #219737

    Purpose

    MoClo-compatible Level 0 - promoter + 5U vector carrying promoter AtPD7

    Depositor

    Alexander Mishin

    Article

    Shakhova et al Int J Mol Sci. 2022 Dec 6;23(23):15441.

    Insert

    promoter of serine carboxypeptidase-like gene AtSCPL30, AtPD7
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    AtPD7

    Available since

    July 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge