We narrowed to 14,168 results for: eng

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pRGEB34

Plasmid

#158152

Purpose

Construction of inPTG-Cas9 plasmids with a truncated 5'-UTR intron for plant genome editing

Depositor

Kabin Xie

Publication

Ding et al Mol Plant. 2018 Apr 2;11(4):542-552. doi

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available since

Nov. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pRSET his-mRFP

Plasmid

#113553

Purpose

For bacterial expression and purification of mRFP

Depositor

Jeanne Stachowiak

Publication

DeGroot et al Biophys J. 2018 Mar 27;114(6):1377-1388.

Insert

his-mRFP

Expression

Bacterial

Available since

July 25, 2018

Availability

Academic Institutions and Nonprofits only

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pDM1018

Plasmid

#108975

Purpose

shuttle vector, N-terminal mCherry tagging

Depositor

Rob Kay

Publication

Paschke et al PLoS One. 2018 May 30;13(5):e0196809. do

Type

Empty backbone

Use

Dictyostelium discoideum shuttle vector

Tags

mCherry

Available since

July 18, 2018

Availability

Academic Institutions and Nonprofits only

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pDM1021

Plasmid

#108978

Purpose

shuttle vector, C-terminal GFP tagging

Depositor

Rob Kay

Publication

Paschke et al PLoS One. 2018 May 30;13(5):e0196809. do

Type

Empty backbone

Use

Dictyostelium discoideum shuttle vector

Tags

GFP

Available since

June 22, 2018

Availability

Academic Institutions and Nonprofits only

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pMOD_C3013

Plasmid

#91100

Purpose

Module C, Promoter: PvUbi2, Gene: GFP (+ BaeI site for GT donor cloning), Terminator: pea rbcsE9

Depositor

Daniel Voytas

Publication

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

GFP (+ BaeI site for GT donor cloning)

Use

Unspecified

Promoter

PvUbi2

Available since

July 14, 2017

Availability

Academic Institutions and Nonprofits only

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pMOD_A0201

Plasmid

#91002

Purpose

Module A, Promoter: 35S, Gene: AtCas9_D10A nickase, Terminator: HSP

Depositor

Daniel Voytas

Publication

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

AtCas9_D10A nickase

Use

CRISPR

Mutation

D10A

Promoter

35S

Available since

July 7, 2017

Availability

Academic Institutions and Nonprofits only

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pMOD_A0202

Plasmid

#91003

Purpose

Module A, Promoter: AtUbi10, Gene: AtCas9_D10A nickase, Terminator: HSP

Depositor

Daniel Voytas

Publication

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

AtCas9_D10A nickase

Use

CRISPR

Mutation

D10A

Promoter

AtUbi10

Available since

July 7, 2017

Availability

Academic Institutions and Nonprofits only

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pNBU2_erm_P1T_DP-rpiL*

Plasmid

#90320

Purpose

Integration vector at an attB site in Bacteroides; contains the P1T_DP aTC-inducible promoter with the RBS rpiL*

Depositor

Andrew Goodman

Publication

Lim et al Cell. 2017 Apr 20;169(3):547-558.e15. do

Type

Empty backbone

Expression

Bacterial

Available since

May 23, 2017

Availability

Academic Institutions and Nonprofits only

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pMJ915v2 + sfGFP

Plasmid

#88918

Purpose

For expression of modified SpyCas9 in e.coli.

Depositor

Jennifer Doudna

Publication

Staahl et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

Inserts

Cas9
T7

Use

CRISPR

Expression

Bacterial

Available since

May 2, 2017

Availability

Academic Institutions and Nonprofits only

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pRVV651

Plasmid

#87631

Purpose

Donor vector for creation of CRISPR/Cas9-mediated Bxb1 attP-flanked alleles.

Depositor

Richard Mann

Publication

Voutev et al Biotechniques. 2017 Jan 1;62(1):37-38. d

Insert

Bxb1 attP

Use

CRISPR

Available since

March 21, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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Rb379-928/C706F-pGex2TK

Plasmid

#21750

Depositor

William Kaelin

Publication

Kaelin et al Cell. 1992 Jul 24. 70(2):351-64.

Insert

Rb (RB1 Human)

Tags

GST

Expression

Bacterial

Mutation

deletion mutant ORF contains aa 379-928 of wt Rb …

Available since

Sept. 30, 2009

Availability

Academic Institutions and Nonprofits only

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pCMV-haA3A-CBE-VA

Plasmid

#220893

Purpose

Express haA3A-CBE-VA (with SpCas9) in mammalian cells

Depositor

Dali Li

Publication

Yang et al Nat Chem Biol. 2024 Mar 29. doi: 10.1038

Insert

haA3A-CBE-VA

Expression

Mammalian

Available since

July 26, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-haA3A-CBE-G-NG

Plasmid

#220897

Purpose

Express haA3A-CBE-G-NG (with SpCas9-NG variant) in mammalian cells

Depositor

Dali Li

Publication

Yang et al Nat Chem Biol. 2024 Mar 29. doi: 10.1038

Insert

haA3A-CBE-G-NG

Expression

Mammalian

Available since

July 26, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-haA3A-CBE-A-NG

Plasmid

#220895

Purpose

Express haA3A-CBE-A-NG (with SpCas9-NG variant) in mammalian cells

Depositor

Dali Li

Publication

Yang et al Nat Chem Biol. 2024 Mar 29. doi: 10.1038

Insert

haA3A-CBE-A-NG

Expression

Mammalian

Available since

July 26, 2024

Availability

Academic Institutions and Nonprofits only

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C331

Plasmid

#141123

Purpose

RhlI expressing plasmid

Depositor

Matthew Bennett

Publication

Alnahhas et al Nat Commun. 2020 Jul 21;11(1):3659. doi:

Insert

RhlI (rhlI )

Tags

ssrA degradation tag

Expression

Bacterial

Promoter

engineered lac promoter

Available since

July 24, 2020

Availability

Academic Institutions and Nonprofits only

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pXM03

Plasmid

#121150

Purpose

This plamid was used for biosynthesis of Prenylated 4‑Hydroxybenzoate in E.coli

Depositor

Minjuan Xu

Publication

He et al ACS Synth Biol. 2018 Sep 21;7(9):2094-21

Insert

XimB, IDS2

Use

Synthetic Biology

Available since

April 1, 2019

Availability

Academic Institutions and Nonprofits only

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pET51b(+)_MBP-nLucCP-67/68

Plasmid

#113922

Purpose

Plasmid encoding the bacterial expression of the MBP fused NanoLuc circular permuted between the position 67 and 68

Depositor

Kai Johnsson

Publication

Hiblot et al Angew Chem Int Ed Engl. 2017 Nov 13;56(4

Insert

MBP-NanoLucCP-67/68

Use

Luciferase

Expression

Bacterial

Promoter

T7

Available since

Sept. 14, 2018

Availability

Academic Institutions and Nonprofits only

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TfRΔEctoR1-mRFP-GFPnb

Plasmid

#113555

Purpose

Transmembrane model receptor to probe receptor internalization via clathrin mediated pathway

Depositor

Jeanne Stachowiak

Publication

DeGroot et al Biophys J. 2018 Mar 27;114(6):1377-1388.

Insert

TfRΔEctoR1-mRFP-GFPnb

Expression

Mammalian

Available since

July 25, 2018

Availability

Academic Institutions and Nonprofits only

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pTHSSe_31

Plasmid

#109235

Purpose

PUPsp1 GFP reporter

Depositor

Christopher Voigt

Publication

Segall-Shapiro et al Nat Biotechnol. 2018 Mar 19. pii: nbt.41

Insert

sfGFP

Expression

Bacterial

Promoter

PUPsp1

Available since

July 23, 2018

Availability

Academic Institutions and Nonprofits only

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J23114-mRFP1-331Bb

Plasmid

#78279

Purpose

mRFP1 behind constitutive promoter J23114 in pSEVA331Bb backbone

Depositor

Tom Ellis

Publication

Florea et al Proc Natl Acad Sci U S A. 2016 Jun 14;11

Insert

J23114-mRFP1

Available since

Nov. 10, 2016

Availability

Academic Institutions and Nonprofits only

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