We narrowed to 32,111 results for: eng

1. pX260-U6-DR-BB-DR-Cbh-NLS-hSpCas9-NLS-H1-shorttracr-PGK-puro

   Plasmid

   #42229

   Purpose

   This plasmid separately encodes a human codon-optimized SpCas9, a tracrRNA and customizable crRNA.

   Depositor

   Feng Zhang

   Article

   Cong et al Science. 2013 Jan 3.

   Insert

   humanized S. pyogenes Cas9
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Available Since

   March 7, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. pFW2500

   Plasmid

   #224213

   Purpose

   Bacteroides genomic editing tool; IPTG inducible-Fncpf1 and recT

   Depositor

   Ophelia Venturelli

   Article

   Feng et al Cell Host Microbe. 2022 Feb 9;30(2):200-

   Insert

   Fncpf1 and recT
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Bacterial

   Available Since

   Sept. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pre_IV_CS10_1545_v1

   Plasmid

   #126861

   Purpose

   4x 1545 b long scaffolds divided by Zn-dependent self-cleaving DNAzyme cassettes, from compatibility group 10, made by method IV, yields linear 1033 b long scaffolds after Zn incubation

   Depositor

   Hendrik Dietz

   Article

   Engelhardt et al ACS Nano. 2019 Apr 22. doi: 10.1021/acsn

   Insert

   IV_CS10_1545_v1
   

   Use

   Synthetic Biology

   Available Since

   July 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSFFV-SpyTag-sfCherry2(11)-TagBFP

   Plasmid

   #117485

   Purpose

   Expresses tandem tag (SpyTag002-GG-sfCherry2(11) fused to N-terminal of TagBFP) in mammalian cells

   Depositor

   Bo Huang

   Article

   Feng et al Commun Biol. 2019 Sep 17;2:344. doi: 10.

   Insert

   SpyTag002-GG-sfCherry2(11)
   

   Use

   Lentiviral

   Tags

   TagBFP

   Expression

   Mammalian

   Promoter

   SFFV

   Available Since

   July 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pre_IV_CS10_1033_v1

   Plasmid

   #126860

   Purpose

   4x 1033 b long scaffolds divided by Zn-dependent self-cleaving DNAzyme cassettes, from compatibility group 10, made by method IV, yields linear 1033 b long scaffolds after Zn incubation

   Depositor

   Hendrik Dietz

   Article

   Engelhardt et al ACS Nano. 2019 Apr 22. doi: 10.1021/acsn

   Insert

   IV_CS10_1033_v1
   

   Use

   Synthetic Biology

   Available Since

   July 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pETDuet_sfCherry2(1-10)_sfCherry2(11)-SpyCatcher

   Plasmid

   #117656

   Purpose

   Bacterial expression vector for split sfCherry3 screening. The “sfCherry2(1-10)” and “sfCherry2(11)-SpyCatcher002” are expressed from two different T7 promoters.

   Depositor

   Bo Huang

   Article

   Feng et al Commun Biol. 2019 Sep 17;2:344. doi: 10.

   Inserts

   sfCherry2(1-10)
   sfCherry2(11)-SpyCatcher002
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   July 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.1(+)_SpyCatcher-6aa-sfCherry2(1-10)

   Plasmid

   #117484

   Purpose

   Expresses tandem binder (SpyCatcher002-6aa-sfCherry2(1-10)) in mammalian cells

   Depositor

   Bo Huang

   Article

   Feng et al Commun Biol. 2019 Sep 17;2:344. doi: 10.

   Insert

   SpyCatcher002-6aa-sfCherry2(1-10)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pJH4783

   Plasmid

   #200785

   Purpose

   Pnpr-4 snb-1::pHluorin unc-54 3' UTR C.elegans AVA and other neurons expression of snb pHluorin

   Depositor

   Mei Zhen

   Article

   Meng et al Sci Adv. 2024 Apr 12;10(15):eadk0002. do

   Insert

   snb-1 (snb-1 Nematode)
   

   Tags

   pHiuorin

   Expression

   Worm

   Promoter

   Pnpr-4

   Available Since

   May 7, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. pcDNA3.1-hAsCpf1(TATV) (pY220)

   Plasmid

   #89353

   Purpose

   Expresses humanized AsCpf1 variant that recognizes TATV PAMs

   Depositor

   Feng Zhang

   Article

   Gao et al Nat Biotechnol. 2017 Jun 5. doi: 10.1038

   Insert

   Cpf1, RVR variant
   

   Use

   CRISPR

   Tags

   NLS and NLS-3xHA

   Expression

   Mammalian

   Mutation

   S542R/K548V/N552R

   Promoter

   CMV

   Available Since

   June 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBiFC-bJunVN173

    Plasmid

    #22012

    Depositor

    Chang-Deng Hu

    Article

    Shyu et al Biotechniques. 2006 Jan . 40(1):61-6.

    Insert

    bJunVN173 (Jun Aequorea Victoria, Rat)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    c-Jun(257-338)-Venus(1-172)

    Available Since

    Sept. 28, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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11. pILGFPB5A

    Plasmid

    #83556

    Purpose

    Used to evaluate the expression output of GAL1 promoter with yEGFP used as the reporter gene.

    Depositor

    Claudia Vickers

    Article

    Peng et al Microb Cell Fact. 2015 Jun 26;14:91. doi

    Insert

    GAL1 promoter-yEGFP
    

    Expression

    Yeast

    Promoter

    GAL1

    Available Since

    Dec. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGL4.R_TRE_minPro

    Plasmid

    #211517

    Purpose

    Dual-luciferase reporter plasmid backbone for insertion of your TRE of interest driving minPro-luc2P expression in addition to constitutive SV-40-driven Renilla luciferase expression

    Depositor

    Justin English

    Article

    Zahm et al Nat Commun. 2024 Nov 28;15(1):10353. doi

    Type

    Empty backbone

    Expression

    Bacterial

    Promoter

    SV40 and minPro

    Available Since

    Jan. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. 3544 pMIG Bcl-2

    Plasmid

    #8793

    Depositor

    Stanley Korsmeyer

    Article

    Cheng et al Mol Cell 2001 Sep;8(3):705-11.

    Insert

    Bcl-2 (BCL2 Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    Nov. 2, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCAGGS_cAMPFIRE-H

    Plasmid

    #182285

    Purpose

    Mammalian expression of the cAMP sensor cAMPFIRE-H under a CAG promotor w/ WPRE translation enhancer.

    Depositor

    Haining Zhong

    Article

    Massengill et al Nat Methods. 2022 Nov;19(11):1461-1471.

    Insert

    cAMPFIRE-H (cAMP sensor)
    

    Expression

    Mammalian

    Available Since

    March 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pILGFP92

    Plasmid

    #83549

    Purpose

    Used to evaluate the expression output of TDH3 promoter with yEGFP used as the reporter gene.

    Depositor

    Claudia Vickers

    Article

    Peng et al Microb Cell Fact. 2015 Jun 26;14:91. doi

    Insert

    TDH3 promoter-yEGFP
    

    Expression

    Yeast

    Promoter

    TDH3

    Available Since

    Oct. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLS-CMV-mP-Rluc

    Plasmid

    #213507

    Purpose

    CMV-driven Renilla luciferase expression vector for luciferase lentivirus-based reporter assay

    Depositor

    Nadav Ahituv

    Article

    Deng et al Science. 2024 May 24;384(6698):eadh0559.

    Insert

    Luciferase
    

    Use

    Lentiviral

    Available Since

    April 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGL4.R_TRE_minTK

    Plasmid

    #211516

    Purpose

    Dual-luciferase reporter plasmid backbone for insertion of your TRE of interest driving minTK-luc2P expression in addition to constitutive SV-40-driven Renilla luciferase expression

    Depositor

    Justin English

    Article

    Zahm et al Nat Commun. 2024 Nov 28;15(1):10353. doi

    Type

    Empty backbone

    Expression

    Bacterial

    Promoter

    SV40 and minTK

    Available Since

    Jan. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. tetO7_Ef1a_SOX2

    Plasmid

    #18919

    Depositor

    Linzhao Cheng

    Article

    Mali et al Stem Cells. 2008 Aug . 26(8):1998-2005.

    Insert

    SOX2 (SOX2 Human)
    

    Use

    Cre/Lox and Lentiviral

    Expression

    Mammalian

    Available Since

    Sept. 4, 2008

    Availability

    Academic Institutions and Nonprofits only

19. pEGFP hsp70 K71E

    Plasmid

    #15216

    Depositor

    Lois Greene

    Article

    Zeng et al J Cell Sci. 2004 Oct 1. 117(Pt 21):4991-

    Insert

    hsp70 K71E (HSPA1A Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    K71E, ATPase-deficient mutant.

    Available Since

    June 15, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSMOX-mNG::LacI-V3

    Plasmid

    #210224

    Purpose

    Integrates a T7 RNA polymerase, tetracycline repressor and an mNeongreen Lac repressor fusion protein with a puromycin drug selection marker into the tubulin locus.

    Depositor

    Calvin Tiengwe

    Article

    Budzak et al Mol Biochem Parasitol. 2023 Dec;256:1115

    Insert

    mNeongreen Lac repressor fusion protein
    

    Use

    Trypanosoma brucei expression

    Promoter

    Pol II readthrough transcription after integration

    Available Since

    Nov. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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