We narrowed to 22,195 results for: his

1. pQE60 P123G wgMDH

   Plasmid

   #204256

   Purpose

   Bacterial expression of P123G wgMDH with IPTG induction - Active site loop mutant

   Depositor

   Malate dehydrogenase CUREs Community (MCC)

   Article

   Cox et al FEBS J. 2005 Feb;272(3):643-54. doi: 10.

   Insert

   MDHG_CITLA
   

   Use

   Tags

   6X His

   Expression

   Bacterial

   Mutation

   P123G

   Promoter

   Available Since

   Oct. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pQE60 P123F wgMDH

   Plasmid

   #204255

   Purpose

   Bacterial expression of P123F wgMDH with IPTG induction - Active site loop mutant

   Depositor

   Malate dehydrogenase CUREs Community (MCC)

   Article

   Cox et al FEBS J. 2005 Feb;272(3):643-54. doi: 10.

   Insert

   MDHG_CITLA
   

   Use

   Tags

   6X His

   Expression

   Bacterial

   Mutation

   P123F

   Promoter

   Available Since

   Oct. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pcS-RDG-C1C2

   Plasmid

   #200163

   Purpose

   This plasmid encodes non-binder control monobody (RDG) fused to the extracellular vesicle binding domain (C1C2) of lactadherin for surface engineering extracellular vesicles.

   Depositor

   Masako Harada

   Article

   Komuro et al Bioengineering (Basel). 2022 Jan 29;9(2)

   Inserts

   RDG-C1C2
   C1C2 domain of lactadherin
   

   Use

   Tags

   HA and HIS

   Expression

   Mammalian

   Mutation

   sequence adopted from doi: 10.1371/journal.pone.0…

   Promoter

   Available Since

   Sept. 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pET28 bleach.2

   Plasmid

   #191878

   Purpose

   Encodes a GFP design, bleach.2

   Depositor

   Sarel Fleishman

   Article

   Weinstein et al Nat Commun. 2023 May 20;14(1):2890. doi:

   Insert

   bleach.2
   

   Use

   Tags

   His Tag

   Expression

   Bacterial

   Mutation

   V61L T65S V68A S72A V112I Y145M

   Promoter

   T7

   Available Since

   Sept. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pET28 mid.8

   Plasmid

   #191902

   Purpose

   Encodes a GFP design, mid.8

   Depositor

   Sarel Fleishman

   Article

   Weinstein et al Nat Commun. 2023 May 20;14(1):2890. doi:

   Insert

   mid.8
   

   Use

   Tags

   His Tag

   Expression

   Bacterial

   Mutation

   V61A S72T Y145M L220V

   Promoter

   T7

   Available Since

   Sept. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pET28 sf:bleach.1

   Plasmid

   #191929

   Purpose

   Encodes a GFP design, sf:bleach.1

   Depositor

   Sarel Fleishman

   Article

   Weinstein et al Nat Commun. 2023 May 20;14(1):2890. doi:

   Insert

   sf:bleach.1
   

   Use

   Tags

   His Tag

   Expression

   Bacterial

   Mutation

   T108L Y145M T167V L220V

   Promoter

   T7

   Available Since

   Dec. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pET28 mid.13

   Plasmid

   #191915

   Purpose

   Encodes a GFP design, mid.13

   Depositor

   Sarel Fleishman

   Article

   Weinstein et al Nat Commun. 2023 May 20;14(1):2890. doi:

   Insert

   mid.13
   

   Use

   Tags

   His Tag

   Expression

   Bacterial

   Mutation

   Q69L S72V Y145S

   Promoter

   T7

   Available Since

   Dec. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pET28 mid.6

   Plasmid

   #191899

   Purpose

   Encodes a GFP design, mid.6

   Depositor

   Sarel Fleishman

   Article

   Weinstein et al Nat Commun. 2023 May 20;14(1):2890. doi:

   Insert

   mid.6
   

   Use

   Tags

   His Tag

   Expression

   Bacterial

   Mutation

   V68M S72T Y145M V224I

   Promoter

   T7

   Available Since

   Dec. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pET28 mid.5

   Plasmid

   #191896

   Purpose

   Encodes a GFP design, mid.5

   Depositor

   Sarel Fleishman

   Article

   Weinstein et al Nat Commun. 2023 May 20;14(1):2890. doi:

   Insert

   mid.5
   

   Use

   Tags

   His Tag

   Expression

   Bacterial

   Mutation

   Y145M T167V H181Y L220V V224I

   Promoter

   T7

   Available Since

   Dec. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET28 mid.4

    Plasmid

    #191895

    Purpose

    Encodes a GFP design, mid.4

    Depositor

    Sarel Fleishman

    Article

    Weinstein et al Nat Commun. 2023 May 20;14(1):2890. doi:

    Insert

    mid.4
    

    Use

    Tags

    His Tag

    Expression

    Bacterial

    Mutation

    V68A S72A Y145M L220V

    Promoter

    T7

    Available Since

    Dec. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pET28 sf:bleach.3

    Plasmid

    #191925

    Purpose

    Encodes a GFP design, sf:bleach.3

    Depositor

    Sarel Fleishman

    Article

    Weinstein et al Nat Commun. 2023 May 20;14(1):2890. doi:

    Insert

    sf:bleach.3
    

    Use

    Tags

    His Tag

    Expression

    Bacterial

    Mutation

    S72A V112I Y145M T167V H181I V224I

    Promoter

    T7

    Available Since

    Dec. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pET28 bleach.3

    Plasmid

    #191914

    Purpose

    Encodes a GFP design, bleach.3

    Depositor

    Sarel Fleishman

    Article

    Weinstein et al Nat Commun. 2023 May 20;14(1):2890. doi:

    Insert

    bleach.3
    

    Use

    Tags

    His Tag

    Expression

    Bacterial

    Mutation

    S72A V112I Y145M T167V H181I V224I

    Promoter

    T7

    Available Since

    Dec. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pET28 mid.12

    Plasmid

    #191913

    Purpose

    Encodes a GFP design, mid.12

    Depositor

    Sarel Fleishman

    Article

    Weinstein et al Nat Commun. 2023 May 20;14(1):2890. doi:

    Insert

    mid.12
    

    Use

    Tags

    His Tag

    Expression

    Bacterial

    Mutation

    T65S S72V Y145M T167V H181I V224I

    Promoter

    T7

    Available Since

    Dec. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pET28 acid.5

    Plasmid

    #191903

    Purpose

    Encodes a GFP design, acid.5

    Depositor

    Sarel Fleishman

    Article

    Weinstein et al Nat Commun. 2023 May 20;14(1):2890. doi:

    Insert

    acid.5
    

    Use

    Tags

    His Tag

    Expression

    Bacterial

    Mutation

    S72T Y145M T167V H181V

    Promoter

    T7

    Available Since

    Dec. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pET28 acid.4

    Plasmid

    #191898

    Purpose

    Encodes a GFP design, acid.4

    Depositor

    Sarel Fleishman

    Article

    Weinstein et al Nat Commun. 2023 May 20;14(1):2890. doi:

    Insert

    acid.4
    

    Use

    Tags

    His Tag

    Expression

    Bacterial

    Mutation

    V61L Q69L Y145M L220V

    Promoter

    T7

    Available Since

    Dec. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pET28 acid.3

    Plasmid

    #191897

    Purpose

    Encodes a GFP design, acid.3

    Depositor

    Sarel Fleishman

    Article

    Weinstein et al Nat Commun. 2023 May 20;14(1):2890. doi:

    Insert

    acid.3
    

    Use

    Tags

    His Tag

    Expression

    Bacterial

    Mutation

    T65S Q69L S72A T108V Y145M V224I

    Promoter

    T7

    Available Since

    Dec. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pET28 sf:mid.10

    Plasmid

    #191934

    Purpose

    Encodes a GFP design, sf:mid.10

    Depositor

    Sarel Fleishman

    Article

    Weinstein et al Nat Commun. 2023 May 20;14(1):2890. doi:

    Insert

    sf:mid.10
    

    Use

    Tags

    His Tag

    Expression

    Bacterial

    Mutation

    L42V Q69L S72A Y145F T167I L220V V224I

    Promoter

    T7

    Available Since

    Dec. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pET28 bleach.1

    Plasmid

    #191885

    Purpose

    Encodes a GFP design, bleach.1

    Depositor

    Sarel Fleishman

    Article

    Weinstein et al Nat Commun. 2023 May 20;14(1):2890. doi:

    Insert

    bleach.1
    

    Use

    Tags

    His Tag

    Expression

    Bacterial

    Mutation

    T108L Y145M T167V L220V

    Promoter

    T7

    Available Since

    Dec. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET28 mid.3

    Plasmid

    #191884

    Purpose

    Encodes a GFP design, mid.3

    Depositor

    Sarel Fleishman

    Article

    Weinstein et al Nat Commun. 2023 May 20;14(1):2890. doi:

    Insert

    mid.3
    

    Use

    Tags

    His Tag

    Expression

    Bacterial

    Mutation

    S72A T108V Y145M T167V H181I V224I

    Promoter

    T7

    Available Since

    Dec. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET28 thermo.9

    Plasmid

    #191882

    Purpose

    Encodes a GFP design, thermo.9

    Depositor

    Sarel Fleishman

    Article

    Weinstein et al Nat Commun. 2023 May 20;14(1):2890. doi:

    Insert

    thermo.9
    

    Use

    Tags

    His Tag

    Expression

    Bacterial

    Mutation

    T167V T203H E222Q

    Promoter

    T7

    Available Since

    Dec. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pET28 basic.3

    Plasmid

    #191881

    Purpose

    Encodes a GFP design, basic.3

    Depositor

    Sarel Fleishman

    Article

    Weinstein et al Nat Commun. 2023 May 20;14(1):2890. doi:

    Insert

    basic.3
    

    Use

    Tags

    His Tag

    Expression

    Bacterial

    Mutation

    T65S V68A S72A Y145S L220V V224I

    Promoter

    T7

    Available Since

    Dec. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pET28 mid.2

    Plasmid

    #191880

    Purpose

    Encodes a GFP design, mid.2

    Depositor

    Sarel Fleishman

    Article

    Weinstein et al Nat Commun. 2023 May 20;14(1):2890. doi:

    Insert

    mid.2
    

    Use

    Tags

    His Tag

    Expression

    Bacterial

    Mutation

    T108I Y145I

    Promoter

    T7

    Available Since

    Dec. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pET28 bleach.6

    Plasmid

    #191879

    Purpose

    Encodes a GFP design, bleach.6

    Depositor

    Sarel Fleishman

    Article

    Weinstein et al Nat Commun. 2023 May 20;14(1):2890. doi:

    Insert

    bleach.6
    

    Use

    Tags

    His Tag

    Expression

    Bacterial

    Mutation

    V61L S72C Y145M H181L V224I

    Promoter

    T7

    Available Since

    Dec. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pET28 slow.3

    Plasmid

    #191877

    Purpose

    Encodes a GFP design, slow.3

    Depositor

    Sarel Fleishman

    Article

    Weinstein et al Nat Commun. 2023 May 20;14(1):2890. doi:

    Insert

    slow.3
    

    Use

    Tags

    His Tag

    Expression

    Bacterial

    Mutation

    Q69L S72V Y145S T167V L220V V224I

    Promoter

    T7

    Available Since

    Dec. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pET28 basic.2

    Plasmid

    #191911

    Purpose

    Encodes a GFP design, basic.2

    Depositor

    Sarel Fleishman

    Article

    Weinstein et al Nat Commun. 2023 May 20;14(1):2890. doi:

    Insert

    basic.2
    

    Use

    Tags

    His Tag

    Expression

    Bacterial

    Mutation

    S72A V112I Y145V L220V V224I

    Promoter

    T7

    Available Since

    Nov. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pET-PLS-KP3-C93A-C96A_rpoA-U

    Plasmid

    #190962

    Purpose

    Test the U-to-C editing activity of KP3:C93A/C96A on AtrpoA editing site, in E. coli

    Depositor

    Bernard Gutmann

    Article

    Ichinose et al Commun Biol. 2022 Sep 15;5(1):968. doi:

    Insert

    PLS-KP3
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    DYW-KP:C93A/C96A

    Promoter

    T7

    Available Since

    Oct. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pET-PLS-KP6-C93A-C96A_rpoA-C

    Plasmid

    #190970

    Purpose

    Test the C-to-U editing activity of KP6:C93A/C96A on AtrpoA editing site, in E. coli

    Depositor

    Bernard Gutmann

    Article

    Ichinose et al Commun Biol. 2022 Sep 15;5(1):968. doi:

    Insert

    PLS-KP6
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    DYW-KP:C93A/C96A

    Promoter

    T7

    Available Since

    Oct. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. PM18033-PLS-KP6-C93A-C96A_rpoA-U

    Plasmid

    #190991

    Purpose

    Test the U-to-C editing activity of KP6:C93A/C96A on AtrpoA editing site, in human cells

    Depositor

    Bernard Gutmann

    Article

    Ichinose et al Commun Biol. 2022 Sep 15;5(1):968. doi:

    Insert

    PLS-KP6
    

    Use

    Tags

    His

    Expression

    Mammalian

    Mutation

    DYW-KP:C93A/C96A

    Promoter

    CMV

    Available Since

    Oct. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pET-PLS-KP2-C93A-C96A_rpoA-U

    Plasmid

    #190958

    Purpose

    Test the U-to-C editing activity of KP2:C93A/C96A on AtrpoA editing site, in E. coli

    Depositor

    Bernard Gutmann

    Article

    Ichinose et al Commun Biol. 2022 Sep 15;5(1):968. doi:

    Insert

    PLS-KP2
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    DYW-KP:C93A/C96A

    Promoter

    T7

    Available Since

    Oct. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. PM18033-PLS-KP3-C93A-C96A_rpoA-U

    Plasmid

    #190982

    Purpose

    Test the U-to-C editing activity of KP3:C93A/C96A on AtrpoA editing site, in human cells

    Depositor

    Bernard Gutmann

    Article

    Ichinose et al Commun Biol. 2022 Sep 15;5(1):968. doi:

    Insert

    PLS-KP3
    

    Use

    Tags

    His

    Expression

    Mammalian

    Mutation

    DYW-KP:C93A/C96A

    Promoter

    CMV

    Available Since

    Oct. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pET-PLS-KP6-C93A-C96A_rpoA-U

    Plasmid

    #190971

    Purpose

    Test the U-to-C editing activity of KP6:C93A/C96A on AtrpoA editing site, in E. coli

    Depositor

    Bernard Gutmann

    Article

    Ichinose et al Commun Biol. 2022 Sep 15;5(1):968. doi:

    Insert

    PLS-KP6
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    DYW-KP:C93A/C96A

    Promoter

    T7

    Available Since

    Oct. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. PM18033-PLS-KP6-C93A-C96A_rpoA-C

    Plasmid

    #190990

    Purpose

    Test the C-to-U editing activity of KP6:C93A/C96A on AtrpoA editing site, in human cells

    Depositor

    Bernard Gutmann

    Article

    Ichinose et al Commun Biol. 2022 Sep 15;5(1):968. doi:

    Insert

    PLS-KP6
    

    Use

    Tags

    His

    Expression

    Mammalian

    Mutation

    DYW-KP:C93A/C96A

    Promoter

    CMV

    Available Since

    Oct. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. PM18033-PLS-KP2-C93A-C96A_rpoA-U

    Plasmid

    #190978

    Purpose

    Test the U-to-C editing activity of KP2:C93A/C96A on AtrpoA editing site, in human cells

    Depositor

    Bernard Gutmann

    Article

    Ichinose et al Commun Biol. 2022 Sep 15;5(1):968. doi:

    Insert

    PLS-KP2
    

    Use

    Tags

    His

    Expression

    Mammalian

    Mutation

    DYW-KP:C93A/C96A

    Promoter

    CMV

    Available Since

    Oct. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. ACE2-IgFc probe

    Plasmid

    #184787

    Purpose

    Expression of His-tagged, soluble ACE2-IgFc microbody

    Depositor

    Ajit Chande

    Article

    Mishra et al Front Cell Infect Microbiol. 2021 Apr 23

    Insert

    hACE2-IgFc (ACE2 Synthetic)
    

    Use

    Tags

    6xHis Tag

    Expression

    Mammalian

    Mutation

    Promoter

    EF1-alpha

    Available Since

    Oct. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pET-15b(+) Drosophila melanogaster PRL (Δprenylation motif)

    Plasmid

    #166418

    Purpose

    Express pET-15b(+) Drosophila melanogaster PRL (Δprenylation motif)

    Depositor

    Kalle Gehring

    Article

    Fakih et al J Biol Chem. 2023 Apr;299(4):103055. doi

    Insert

    Drosophila melanogaster PRL (Prl Fly)
    

    Use

    Tags

    N-terminal His-tag

    Expression

    Bacterial

    Mutation

    Deleted nucleotides coding for 4 C-terminal amino…

    Promoter

    AmpR, lac1

    Available Since

    Sept. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pET303-ColE1-T-P110A

    Plasmid

    #180237

    Purpose

    Expresses T domain of Colicin E1 (residues 1-190) with proline to alanine mutation at residue 110

    Depositor

    Joanna Slusky

    Article

    Budiardjo et al Elife. 2022 Feb 24;11. pii: 73297. doi:

    Insert

    Colicin E1
    

    Use

    Tags

    6x His-tag

    Expression

    Bacterial

    Mutation

    Truncation of ColE1 containing T domain of ColE1 …

    Promoter

    T7

    Available Since

    Aug. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pEUK028

    Plasmid

    #180826

    Purpose

    T7-inducible expression construct to produce SwGdmA (SWIT_RS16490) in E. coli

    Depositor

    Gregg Beckham

    Article

    Bleem et al Chem Catalysis, 2022

    Insert

    SwGdmA (SWIT_RS16490 Sphingomonas wittichii RW1, Synthetic)
    

    Use

    Tags

    6X His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    June 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pEUK037

    Plasmid

    #180829

    Purpose

    T7-inducible expression construct to produce CnGdmB (CNE_RS35790) in E. coli

    Depositor

    Gregg Beckham

    Article

    Bleem et al Chem Catalysis, 2022

    Insert

    CnGdmB (CNE_RS35790 Cupriavidus necator N-1)
    

    Use

    Tags

    6X His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    June 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pET-UTX(420-633)-H6

    Plasmid

    #183773

    Purpose

    Express pET-UTX(420-633)-H6 in E.coli. Purified pET-UTX(420-633)-H6 was used for generating antibodies in rabbits.

    Depositor

    Peter Harte

    Article

    Tie et al Mol Cell Biol. 2012 Jun;32(12):2323-34.

    Insert

    UTX
    

    Use

    Tags

    6 His tag at the C-terminus

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    April 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. CTAG_eGFP-GHtag-HDEL

    Plasmid

    #136096

    Purpose

    FP with ER retention signal for C-term fusion with CDS12 with ER targeting signal peptide. Contains Gly-His tag

    Depositor

    Jim Haseloff

    Article

    Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

    Insert

    CTAG_eGFP(noATG,noStop)-3xGly5xHys-HDEL
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    BsaI/ SapI domesticated

    Promoter

    Available Since

    Nov. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge