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ABE-RA3.3

Plasmid

#208304

Purpose

Expresses ABE-RA3.3 in mammalian cells

Depositor

Weixin Tang

Article

Xiao et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

Insert

TadA-RA3.3-SpCas9 D10A

Expression

Mammalian

Mutation

E27D, R47K, P48A, R51H, I76F, D108G, K110R, A114V…

Available Since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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ABE-RA4.0

Plasmid

#208305

Purpose

Expresses ABE-RA4.0 in mammalian cells

Depositor

Weixin Tang

Article

Xiao et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

Insert

TadA-RA4.0-SpCas9 D10A

Expression

Mammalian

Mutation

P48A, R51H, I76F, A106V, D108G, K110R, T111H, D11…

Available Since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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PlayBack-CMV-Nhe1

Plasmid

#203304

Purpose

For expression of eukaryotic genes of interest with CMV promoter with NheI as a PlayBack Restriction Enzyme

Depositor

Aleksandar Necakov

Article

Foran et al Biotechniques. 2023 Oct;75(4):168-178. d

Type

Empty backbone

Expression

Mammalian

Available Since

Oct. 16, 2023

Availability

Academic Institutions and Nonprofits only

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PlayBack-CMV-Bgl11

Plasmid

#203306

Purpose

For expression of eukaryotic genes of interest with CMV promoter with BglII as a PlayBack Restriction Enzyme

Depositor

Aleksandar Necakov

Article

Foran et al Biotechniques. 2023 Oct;75(4):168-178. d

Type

Empty backbone

Expression

Mammalian

Available Since

Oct. 16, 2023

Availability

Academic Institutions and Nonprofits only

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PlayBack-Ef1a-Nhe1

Plasmid

#203310

Purpose

For expression of eukaryotic genes of interest with Ef1a promoter with NheI as a PlayBack Restriction Enzyme

Depositor

Aleksandar Necakov

Article

Foran et al Biotechniques. 2023 Oct;75(4):168-178. d

Type

Empty backbone

Expression

Mammalian

Available Since

Oct. 16, 2023

Availability

Academic Institutions and Nonprofits only

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pATT-Dest-Cre

Plasmid

#202714

Purpose

Cre recombinase staging plasmid to make transposon insertion libraries.

Depositor

Mary Dunlop

Article

Tague et al ACS Synth Biol. 2023 Oct 3. doi: 10.1021

Insert

Cre

Use

Staging plasmid to make transposon insertion libr…

Available Since

Oct. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pPOTv7-blast-moxNG

Plasmid

#190222

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Samuel Dean

Article

Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

Insert

moxNeonGreen

Use

Unspecified

Promoter

read through

Available Since

Sept. 8, 2023

Availability

Academic Institutions and Nonprofits only

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1BR9

Plasmid

#193862

Purpose

E. coli expression vector for N-ter GFP11 and C-ter R9 tagged proteins. Empty backbone for LIC cloning.

Depositor

Markita Landry

Article

Wang et al Commun Biol. 2023 Aug 12;6(1):840. doi:

Type

Empty backbone

Tags

GFP11, Hisx6, and R9

Expression

Bacterial

Available Since

Aug. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pPOTv7-blast-Ppyr9eh

Plasmid

#201443

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Samuel Dean

Article

Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

Insert

Ppyr9eh

Use

Unspecified

Promoter

read through

Available Since

July 14, 2023

Availability

Academic Institutions and Nonprofits only

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pPOTv7-blast-mNG:Ppyr9eh

Plasmid

#201441

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Samuel Dean

Article

Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

Insert

mNG:Ppyr9eh

Use

Unspecified

Promoter

read through

Available Since

July 14, 2023

Availability

Academic Institutions and Nonprofits only

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pPOTv6-puro-3Ty:mScarletI:3Ty

Plasmid

#201093

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Samuel Dean

Article

Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

Insert

mScaret-I

Use

Unspecified

Promoter

read through

Available Since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pPOTv6-hygro-3Ty:mScarletI:3Ty

Plasmid

#201092

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Samuel Dean

Article

Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

Insert

mScaret-I

Use

Unspecified

Promoter

read through

Available Since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pPOTv6-g418-His:mScarletI:His

Plasmid

#201098

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Samuel Dean

Article

Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

Insert

mScarlet-I

Use

Unspecified

Promoter

read through

Available Since

July 11, 2023

Availability

Academic Institutions and Nonprofits only

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pPOTv6-g418-His:mNG:His

Plasmid

#201095

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Samuel Dean

Article

Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

Insert

mNeonGreen

Use

Unspecified

Promoter

read through

Available Since

July 11, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pPOTv6-g418-3Ty:mScarletI:3Ty

Plasmid

#201091

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Samuel Dean

Article

Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

Insert

mScaret-I

Use

Unspecified

Promoter

read through

Available Since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pPOTv6-hygro-3Ty:tagRFPt:3Ty

Plasmid

#201088

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Samuel Dean

Article

Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

Insert

tagRFPt

Use

Unspecified

Promoter

read through

Available Since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pPOTv6-g418-3Ty:tagRFPt:3Ty

Plasmid

#201087

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Samuel Dean

Article

Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

Insert

tagRFPt

Use

Unspecified

Promoter

read through

Available Since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pPOTv6-blast-3Ty:tagRFPt:3Ty

Plasmid

#201086

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Samuel Dean

Article

Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

Insert

tagRFPt

Use

Unspecified

Promoter

read through

Available Since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pPOTv7-g418-tagRFPt

Plasmid

#201085

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Samuel Dean

Article

Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

Insert

tagRFPt

Use

Unspecified

Promoter

read through

Available Since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pPOTv7-hygro-tagRFPt

Plasmid

#201084

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Samuel Dean

Article

Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

Insert

tagRFPt

Use

Unspecified

Promoter

read through

Available Since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge