We narrowed to 14,045 results for: crispr grnas

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pGL3-U6-sgRNA-EGFP

Plasmid

#107721

Purpose

For expression of sgRNA from the U6 promoter with EGFP expression simultaneously

Depositor

Xingxu Huang

Article

Liu et al Nat Commun. 2018 Jun 14;9(1):2338. doi:

Type

Empty backbone

Use

CRISPR

Available Since

April 18, 2018

Availability

Academic Institutions and Nonprofits only

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pGL3-U6-sgRNA-BFP

Plasmid

#107722

Purpose

For expression of sgRNA from the U6 promoter with BFP expression simultaneously

Depositor

Xingxu Huang

Article

Liu et al Nat Commun. 2018 Jun 14;9(1):2338. doi:

Type

Empty backbone

Use

CRISPR

Available Since

April 18, 2018

Availability

Academic Institutions and Nonprofits only

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gRNA-HEK3-Puro

Plasmid

#136282

Purpose

Expresses a guide RNA targeting HEK3 site

Depositor

Zhaohui Ye

Article

McGrath et al Nat Commun. 2019 Nov 25;10(1):5353. doi:

Insert

sgRNA-HEK3

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Jan. 31, 2020

Availability

Academic Institutions and Nonprofits only

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px459-Hira KO sgRNA

Plasmid

#186938

Purpose

Hira KO in mouse ES cells

Depositor

Zhiguo Zhang

Article

Xu et al Nat Commun. 2022 May 6;13(1):2514. doi:

Insert

Hira KO sgRNA (Hira Mouse)

Use

CRISPR and Mouse Targeting

Expression

Mammalian

Promoter

U6

Available Since

Aug. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pX459-HypaCas9-TUBA1B_sgRNA

Plasmid

#183889

Purpose

pX459V2.0-HypaCas9 plasmid with TUBA1B sgRNA for N-terminal tagging of alpha-tubulin in human cells.

Depositor

Alisa Piekny

Article

Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

Insert

TUBA1B sgRNA spacer (TUBA1B Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

May 20, 2022

Availability

Academic Institutions and Nonprofits only

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px330-UFSP2 sgRNA2

Plasmid

#134637

Purpose

contains sgRNA targeting human UFSP2 for gene knockout

Depositor

Yihong Ye

Article

Wang et al Cell Res. 2020 Jan;30(1):5-20. doi: 10.1

Insert

UFSP2 sgRNA2 (UFSP2 Human)

Expression

Mammalian

Available Since

Nov. 19, 2019

Availability

Academic Institutions and Nonprofits only

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px459-H3f3a KO sgRNA

Plasmid

#186940

Purpose

H3f3a KO in mouse ES cells

Depositor

Zhiguo Zhang

Article

Xu et al Nat Commun. 2022 May 6;13(1):2514. doi:

Insert

H3f3a KO sgRNA (H3f3a Mouse)

Use

CRISPR and Mouse Targeting

Expression

Mammalian

Promoter

U6

Available Since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

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px459-H3f3b KO sgRNA

Plasmid

#186941

Purpose

H3f3b KO in mouse ES cells

Depositor

Zhiguo Zhang

Article

Xu et al Nat Commun. 2022 May 6;13(1):2514. doi:

Insert

H3f3b KO sgRNA (H3f3b Mouse)

Use

CRISPR and Mouse Targeting

Expression

Mammalian

Promoter

U6

Available Since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

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px330-UFSP2 sgRNA1

Plasmid

#134638

Purpose

contains sgRNA targeting human UFSP2 for gene knockout

Depositor

Yihong Ye

Article

Wang et al Cell Res. 2020 Jan;30(1):5-20. doi: 10.1

Insert

UFSP2 sgRNA1 (UFSP2 Human)

Expression

Mammalian

Available Since

Nov. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pLEW100v5-NEO-T7-sgRNA

Plasmid

#245118

Purpose

Plasmid backbone to for inserting a T7 promoter driven guide RNA into the rDNA spacer of T. brucei

Depositor

Monica Mugnier

Article

Smith et al Nature (2026)

Type

Empty backbone

Use

CRISPR

Available Since

April 8, 2026

Availability

Academic Institutions and Nonprofits only

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pLenti_DualsgRNA_TIMM23_ER-dAPEX-BFP2

Plasmid

#236734

Purpose

Dual sgRNA targeting TIMM23 expressing dAPEX-BFP targeting to ER

Depositor

Derek Narendra

Article

Thayer et al EMBO J. 2025 Nov 20. doi: 10.1038/s44318

Insert

TIMM23 (TIMM23 Human)

Use

CRISPR and Lentiviral

Available Since

Dec. 10, 2025

Availability

Academic Institutions and Nonprofits only

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px459-Daxx KO sgRNA

Plasmid

#186939

Purpose

Daxx KO in mouse ES cells

Depositor

Zhiguo Zhang

Article

Xu et al Nat Commun. 2022 May 6;13(1):2514. doi:

Insert

Daxx KO sgRNA (Daxx Mouse)

Use

CRISPR and Mouse Targeting

Expression

Mammalian

Promoter

U6

Available Since

Aug. 8, 2022

Availability

Academic Institutions and Nonprofits only

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L2_NOP1gRNA-Cas9-CsA

Plasmid

#136139

Purpose

Plasmid with NOP1-gRNA to tranform Mp as CRISPR control. Contains Cas9 and HygR

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

p5-35Sx2:HygR p5-MpU6:NOP1gRNA1 p5-MpEF1a:Cas9-NLS

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available Since

Nov. 30, 2021

Availability

Academic Institutions and Nonprofits only

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EZH2-Promoter-gRNA

Plasmid

#131329

Purpose

gRNA to generate endogenously tagged -tetR-EZH2

Depositor

Danny Reinberg

Article

Oksuz et al Mol Cell. 2018 Jun 21;70(6):1149-1162.e5

Insert

EZH2-Promoter-gRNA

Use

CRISPR

Expression

Mammalian

Available Since

March 6, 2020

Availability

Academic Institutions and Nonprofits only

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pLV hU6-sgRNA Nestin-dCas9-KRAB-T2a-GFP

Plasmid

#196988

Purpose

Derived from pLV hU6-sgRNA hUbC-dCas9-KRAB-T2a-GFP, custom sgRNA cloning vector

Depositor

Elizabeth Kirby

Article

Dause et al Mol Neurobiol. 2025 Mar 25. doi: 10.1007

Insert

Nestin-dCas9-KRAB-T2a-GFP

Use

CRISPR and Lentiviral

Available Since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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hU6 promoter PspCas13b gRNA dual luciferase reporter 3’UTR

Plasmid

#119859

Purpose

gRNA plasmid with hU6 promoter PspCas13b gRNA dual luciferase reporter

Depositor

Bryan Dickinson

Article

Rauch et al J Am Chem Soc. 2018 Sep 26;140(38):11974

Insert

gRNA dual luciferase reporter

Use

CRISPR

Expression

Mammalian

Available Since

Dec. 19, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti-dSaCas9-KRAB-gRNA-TRE-blast

Plasmid

#201151

Purpose

Lentiviral expression of S. aureus dead Cas9 (dCas9/dSaCas9/SadCas9) fused to the KRAB domain as well as a gRNA targeting the tight TRE promoter and the blasticidin resistance gene.

Depositor

Moshe Szyf

Inserts

SadCas9-KRAB
gRNA-TRE

Use

CRISPR and Lentiviral

Tags

HA

Mutation

gRNA sequence: ATCAGTGATAGAGAACGTATG

Available Since

July 25, 2023

Availability

Academic Institutions and Nonprofits only

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7b sgRNA for EJ7-GFP reporter

Plasmid

#113624

Purpose

sgRNA/CAS9 expression plasmid to induce the 3’ double-strand break in the EJ7-GFP reporter

Depositor

Jeremy Stark

Article

Bhargava et al Nat Commun. 2018 Jun 27;9(1):2484. doi:

Insert

7b sgRNA

Use

CRISPR

Expression

Mammalian

Available Since

Aug. 13, 2018

Availability

Academic Institutions and Nonprofits only
pPB_mU6_enh-gRNA_Puro-T2A-BFP

Plasmid

#235571

Purpose

Enhanced guide RNA expression & genomic integration. Target gRNA sequences are cloned in via the BstXI and BlpI sites.

Depositor

Jamie Hackett

Article

Policarpi et al Nat Genet. 2024 Jun;56(6):1168-1180. doi

Insert

sgRNA backbone

Use

CRISPR

Available Since

April 29, 2025

Availability

Academic Institutions and Nonprofits only

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pLenti-U6-epegRNA-SV40-puro

Plasmid

#240539

Purpose

Lentiviral backbone plasmid to insert epegRNAs (tevopreq1) of interest with puromycin resistance. The cloning site is BsmBI.

Depositor

Wensheng Wei

Article

Niu et al Nat Biotechnol. 2025 Jun 24. doi: 10.103

Insert

epegRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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