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pLentiSIV hSyn HA-NLS-SpCas9-NLS WPRE

Plasmid

#236242

Purpose

Lentiviral expression of SpCas9

Depositor

Jennifer Lippincott-Schwartz

Article

Benedetti et al Cell. 2025 Jan 23;188(2):484-500.e22. do

Insert

SpCas9

Use

Lentiviral

Tags

Expression

Mutation

Promoter

human Synapsin 1

Available since

May 1, 2025

Availability

Academic Institutions and Nonprofits only

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PC1575

Plasmid

#232088

Purpose

Mammalian expression plasmid for ubiquibody (uAb) cloning backbone. Includes an Esp3I restriction site directly upstream of GSGSG linker and CHIPΔTPR CDS and a C-terminal IRES-mCherry cassette.

Depositor

Pranam Chatterjee

Article

Bhat et al Science Advances, 22 Jan 2025. Vol 11, I

Insert

uAb with cloning Esp3I restriction site

Use

Tags

IRES-mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 24, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-mGold2s-Lysosome-N-20

Plasmid

#231770

Purpose

Visualization of lysosome in mammalian cells using mGold2s

Depositor

Francois St-Pierre

Article

Lee et al Nat Commun. 2025 Apr 4;16(1):3241. doi:

Insert

mGold2s-Lysosome-N-20 (Lamp1 Rat)

Use

Tags

mGold2s

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-mGold2t-Lysosome-N-20

Plasmid

#231778

Purpose

Visualization of lysosome in mammalian cells using mGold2t

Depositor

Francois St-Pierre

Article

Lee et al Nat Commun. 2025 Apr 4;16(1):3241. doi:

Insert

mGold2t-Lysosome-N-20 (Lamp1 Rat)

Use

Tags

mGold2s

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-mGold2t-Rab4a-C-7

Plasmid

#231781

Purpose

Visualization of endosome in mammalian cells using mGold2t

Depositor

Francois St-Pierre

Article

Lee et al Nat Commun. 2025 Apr 4;16(1):3241. doi:

Insert

mGold2t-Rab4a-C-7 (RAB4A Human)

Use

Tags

mGold2s

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-mGold2s-Rab4a-C-7

Plasmid

#231773

Purpose

Visualization of endosome in mammalian cells using mGold2s

Depositor

Francois St-Pierre

Article

Lee et al Nat Commun. 2025 Apr 4;16(1):3241. doi:

Insert

mGold2s-Rab4a-C-7 (RAB4A Human)

Use

Tags

mGold2s

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPR v2 puro mGSDME gRNA2

Plasmid

#223522

Purpose

Knocking out mGSDME in mouse cells

Depositor

Judy Lieberman

Article

Zhang et al Nature. 2020 Mar;579(7799):415-420. doi:

Insert

GSDME (GSDME Mouse)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 9, 2025

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPR v2 puro mGSDME gRNA1

Plasmid

#223521

Purpose

Knocking out mGSDME in mouse cells

Depositor

Judy Lieberman

Article

Zhang et al Nature. 2020 Mar;579(7799):415-420. doi:

Insert

GSDME (GSDME Mouse)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 9, 2025

Availability

Academic Institutions and Nonprofits only

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pLenti-CMV-GFP-Puro_TAL1-long

Plasmid

#210642

Purpose

Overexpression of TAL1-long

Depositor

Maayan Salton

Article

Sharma et al PLoS Biol. 2023 Jun 28;21(6):e3002175. d

Insert

TAL1-long (TAL1 Human)

Use

Lentiviral

Tags

GFP

Expression

Mutation

Promoter

Available since

Jan. 9, 2025

Availability

Academic Institutions and Nonprofits only

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pET3a.H2B-K120C

Plasmid

#224706

Purpose

Expresses Human H2B K120C mutant in bacterial cells for fluorescent labelling of histone octamers

Depositor

Chen Davidovich

Article

Uckelmann et al bioRxiv [Preprint]. 2024 Feb 12:2023.05.

Insert

H2B-K120C (H2B Human)

Use

Tags

Expression

Bacterial

Mutation

K120C

Promoter

T7

Available since

Oct. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pX330_DAZL_cterm2

Plasmid

#222919

Purpose

Cas9/sgRNA plasmid for targeting DAZL

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2022.07.11.499564

Insert

Cas9, DAZL sgRNA 2 (DAZL Synthetic, Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Aug. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pX330_DAZL_cterm3

Plasmid

#222920

Purpose

Cas9/sgRNA plasmid for targeting DAZL

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2022.07.11.499564

Insert

Cas9, DAZL sgRNA 3 (DAZL Synthetic, Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Aug. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pX330_TFAP2C_cterm

Plasmid

#222916

Purpose

Cas9/sgRNA plasmid for targeting TFAP2C

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2022.07.11.499564

Insert

Cas9, TFAP2C sgRNA (TFAP2C Synthetic, Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Aug. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pX330_FIGLA_cterm4

Plasmid

#222909

Purpose

Cas9/sgRNA plasmid for targeting FIGLA

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2022.07.11.499564

Insert

Cas9, FIGLA sgRNA 4 (FIGLA Synthetic, Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

July 22, 2024

Availability

Academic Institutions and Nonprofits only

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pX330_FIGLA_cterm3

Plasmid

#222908

Purpose

Cas9/sgRNA plasmid for targeting FIGLA

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2022.07.11.499564

Insert

Cas9, FIGLA sgRNA 3 (FIGLA Synthetic, Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

July 22, 2024

Availability

Academic Institutions and Nonprofits only

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pX330_FIGLA_cterm2

Plasmid

#222907

Purpose

Cas9/sgRNA plasmid for targeting FIGLA

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2022.07.11.499564

Insert

Cas9, FIGLA sgRNA 2 (FIGLA Synthetic, Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

July 22, 2024

Availability

Academic Institutions and Nonprofits only

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pX330_FIGLA_cterm1

Plasmid

#222906

Purpose

Cas9/sgRNA plasmid for targeting FIGLA

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2022.07.11.499564

Insert

Cas9, FIGLA sgRNA 1 (FIGLA Synthetic, Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

July 22, 2024

Availability

Academic Institutions and Nonprofits only

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pX330_NANOS3_cterm

Plasmid

#222904

Purpose

Cas9/sgRNA plasmid for targeting NANOS3

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2022.07.11.499564

Insert

Cas9, NANOS3 sgRNA (NANOS3 Synthetic, Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

July 22, 2024

Availability

Academic Institutions and Nonprofits only

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pPB-cT3G-cERP2-FIGLA

Plasmid

#222929

Purpose

PiggyBac transposon plasmid for doxycycline inducible expression of FIGLA

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2022.07.11.499564

Insert

FIGLA (FIGLA Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 22, 2024

Availability

Academic Institutions and Nonprofits only

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pPB-cT3G-cERP2-HHEX

Plasmid

#222928

Purpose

PiggyBac transposon plasmid for doxycycline inducible expression of HHEX

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2022.07.11.499564

Insert

HHEX (HHEX Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 22, 2024

Availability

Academic Institutions and Nonprofits only

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