We narrowed to 10,454 results for: ESP

1. DKC1 F2.3 gRNA

   Plasmid

   #90653

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human DKC1

   Depositor

   Iain Cheeseman

   Article

   McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

   Insert

   DKC1 (Guide Designation F2.3)
   

   Use

   CRISPR and Lentiviral

   Promoter

   U6

   Available Since

   May 31, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA5FRT/TO-UBE2QL1-Strep/HA

   Plasmid

   #124666

   Purpose

   Expresses UBE2QL1 with C-terminal Strep-HA tag in mammalian cells

   Depositor

   Hemmo Meyer

   Article

   Koerver et al EMBO Rep. 2019 Aug 21:e48014. doi: 10.15

   Insert

   UBE2QL1 (UBE2QL1 Human)
   

   Tags

   2xStrep/HA

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. mEos3.2GPI

   Plasmid

   #203759

   Purpose

   Encodes the fluorescent protein mEos3.2 followed by the C-terminal sequence derived from CD58 encoding a GPI-attachment signal. Used as a fluorescent plasma membrane probe.

   Depositor

   Sarah Veatch

   Article

   Shelby et al Nat Chem Biol. 2023 Jun;19(6):750-758. d

   Insert

   CD58 c-term (CD58 Human)
   

   Tags

   mEos3.2

   Expression

   Mammalian

   Available Since

   Aug. 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. AAVS1_puro_CAG_HexaPro

   Plasmid

   #164077

   Purpose

   Targeted integration of SARS-CoV-2 spike HexaPro variant into the AAVS1 genomic safe harbor locus

   Depositor

   Yannick Doyon

   Article

   Rivest et al protocols.io 2021

   Insert

   SARS-CoV-2 S HexaPro (S Severe acute respiratory syndrome coronavirus 2)
   

   Use

   CRISPR

   Tags

   2X Strep-Tag II, 8X His tag, and HRV 3C cleavage …

   Expression

   Mammalian

   Mutation

   Ectodomain only (AAs 1-1208); 682-685 (furin site…

   Promoter

   CAG

   Available Since

   Jan. 4, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pcDNA5FRT/TO-p37-Strep-HA

   Plasmid

   #113485

   Purpose

   Expression of human p37 with C-terminal strep-HA tag

   Depositor

   Hemmo Meyer

   Article

   Hulsmann et al Mol Cell Proteomics. 2018 Mar 29. pii: R

   Insert

   p37 (UBXN2B Human)
   

   Tags

   2xstrep-HA

   Expression

   Mammalian

   Mutation

   wildtype without stop codon

   Promoter

   CMV

   Available Since

   Aug. 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. SARS_COV2_ORF9B_HA_BASU

   Plasmid

   #153981

   Purpose

   Express Orf9b gene along with HA and BASU

   Depositor

   Paul Khavari

   Article

   Meyers et al PLoS Pathog. 2021 Oct 1;17(10):e1009412.

   Insert

   orf9b
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Oct. 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. FEN1 C10.3 gRNA

   Plasmid

   #90689

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human FEN1

   Depositor

   Iain Cheeseman

   Article

   McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

   Insert

   FEN1 (Guide Designation C10.3)
   

   Use

   CRISPR and Lentiviral

   Promoter

   U6

   Available Since

   July 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA5FRT/TO-UBE2QL1-CS-Strep/HA

   Plasmid

   #129513

   Purpose

   Expresses UBE2QL1 C88S (catalytically inactive mutant) with C-terminal Strep-HA tag in mammalian cells

   Depositor

   Hemmo Meyer

   Article

   Koerver et al EMBO Rep. 2019 Aug 21:e48014. doi: 10.15

   Insert

   UBE2QL1 C88S (UBE2QL1 Human)
   

   Tags

   2xStrep/HA

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA_SZ1-RGG-GFP-RGG

   Plasmid

   #124932

   Purpose

   Expression of SZ1-RGG-GFP-RGG

   Depositor

   Matthew Good, Daniel Hammer, Benjamin Schuster

   Article

   Schuster et al Nat Commun. 2018 Jul 30;9(1):2985. doi:

   Insert

   SYNZIP1-RGG-GFP-RGG (using LAF-1 RGG domain) (laf-1 Nematode)
   

   Tags

   GFP

   Expression

   Mammalian

   Available Since

   June 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMIR-Dsred-IRES-His-Halo-Tev-Keap C288S

    Plasmid

    #62459

    Purpose

    expresses his-tagged C288S human Keap1 protein

    Depositor

    Yimon Aye

    Article

    Parvez et al J Am Chem Soc. 2014 Dec 31.

    Insert

    Keap1 (KEAP1 Human)
    

    Tags

    His-Halo-Tev

    Expression

    Mammalian

    Mutation

    Changed Cysteine 288 of Keap1 to serine

    Promoter

    CMV

    Available Since

    March 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pFRT_TO_eGFP_ELAVL1

    Plasmid

    #106105

    Purpose

    Inducible gene expression of EGFP ELAVL1

    Depositor

    Thomas Tuschl

    Article

    Meyer et al Mol Cell. 2018 Feb 15;69(4):622-635.e6.

    Insert

    NP_001410.2 (ELAVL1 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Available Since

    Feb. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. SMC6 G4.4 gRNA

    Plasmid

    #90899

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human SMC6

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

    Insert

    SMC6 (Guide Designation G4.4)
    

    Use

    CRISPR and Lentiviral

    Promoter

    U6

    Available Since

    Aug. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. SMC6 G3.4 gRNA

    Plasmid

    #90898

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human SMC6

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

    Insert

    SMC6 (Guide Designation G3.4)
    

    Use

    CRISPR and Lentiviral

    Promoter

    U6

    Available Since

    July 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLX302-JSP1-V5 puro

    Plasmid

    #87773

    Purpose

    Expresses V5 tagged human JSP1 (DUSP22)

    Depositor

    Kevin Janes

    Article

    Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

    Insert

    JSP1 (DUSP22 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Available Since

    March 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLHCX-Flag-mPKM2(C358S)

    Plasmid

    #42513

    Depositor

    Dimitrios Anastasiou

    Article

    Anastasiou et al Science. 2011 Dec 2;334(6060):1278-83. d

    Insert

    Pyruvate Kinase M2 Cys358->Ser
    

    Use

    Retroviral

    Tags

    Flag

    Mutation

    Changed Cysteine 358 to Serine

    Promoter

    CMV

    Available Since

    March 19, 2013

    Availability

    Academic Institutions and Nonprofits only

16. 1.5 BV

    Plasmid

    #16048

    Depositor

    Robert Benezra

    Article

    Tournay et al Mol Cell Biol. 1996 May . 16(5):2418-30.

    Insert

    Id1 promoter region (Id1 Mouse)
    

    Use

    Luciferase

    Tags

    Luciferase

    Expression

    Mammalian

    Mutation

    -1585 to +88 of promoter

    Available Since

    Nov. 2, 2007

    Availability

    Academic Institutions and Nonprofits only

17. IRAK1mut-UTR

    Plasmid

    #15096

    Depositor

    David Baltimore

    Article

    Taganov et al Proc Natl Acad Sci U S A. 2006 Aug 15. 1

    Insert

    IRAK1 3'UTR (IRAK1 Human)
    

    Use

    Luciferase

    Expression

    Mammalian

    Mutation

    4-bp substitutions in the miR-146 target sites

    Available Since

    June 6, 2007

    Availability

    Academic Institutions and Nonprofits only

18. pET30b jGCaMP8f F366H

    Plasmid

    #204139

    Purpose

    Production of recombinant calcium sensor jGCaMP8f F366H high dynamic range variant in E.coli

    Depositor

    Katalin Torok

    Article

    Tran et al Front Cell Neurosci. 2023 May 18;17:1155

    Insert

    jGCaMP8f F366H
    

    Tags

    N-terminal His tag

    Expression

    Bacterial

    Mutation

    Phenylalanine 366 changed to histidine

    Promoter

    T7

    Available Since

    Dec. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pRB117.2

    Plasmid

    #181949

    Purpose

    aTc-inducible expression of NarL with N-terminal mNeonGreen fusion. Also contains mCherry under NarL-controlled promoter PdcuS

    Depositor

    Jeffrey Tabor

    Article

    Butcher et al Proc Natl Acad Sci U S A. 2022 Aug 30;11

    Insert

    mNG-NarL; mCherry; tetR (narL, AX04_RS23485 narL - E. coli, Synthetic)
    

    Tags

    mNeonGreen

    Expression

    Bacterial

    Mutation

    See Depositor Comments Below

    Promoter

    mNG-NarL-PLtetO-1; mCherry-PdcuS; tetR-J23106

    Available Since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCDNAIIIB cdc42 QL

    Plasmid

    #61636

    Purpose

    mammalian expression of cdc42 constitutively active mutant

    Depositor

    JS Gutkind

    Article

    Coso et al Cell. 1995 Jun 30;81(7):1137-46.

    Insert

    Cdc42 (CDC42 Human)
    

    Expression

    Mammalian

    Mutation

    Q61L, constitutively active

    Promoter

    CMV

    Available Since

    Jan. 26, 2015

    Availability

    Academic Institutions and Nonprofits only